electrocompetent top10 cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher electrocompetent top10 cells
    Electrocompetent Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrocompetent top10 cells/product/Thermo Fisher
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    electrocompetent top10 cells - by Bioz Stars, 2020-03
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Extracting single genomes from heterogenous DNA samples: A test case with Carsonella ruddii, the bacterial symbiont of psyllids (Insecta)
    Article Snippet: .. TOPO cloning reactions were transformed into electrocompetent TOP10 cells (Invitrogen) to achieve a high yield of recombinant clones. .. DNA sequencing and data analysis Plasmid DNA inserts from the TOPO cloning reactions were sequenced at the University of Arizona sequencing facility, using M13 forward and reverse primers.

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: .. The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. DNA from colonies was amplified using M13 primers and labeled using BigDye V3.0 (Applied Biosystems, Foster City, CA).

    Amplification:

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: PCR amplification of Brahma was accomplished using High-Fidelity Platinum Taq Polymerase (Invitrogen, Carlsbad, CA). .. The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ).

    Agarose Gel Electrophoresis:

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: Fragments were separated by agarose gel electrophoresis, and DNA was extracted by using a QIAquick gel extraction kit (QIAGEN, Valencia, Calif.). .. Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.).

    Methylation:

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. The methylation status of CpG sites from each clone was analyzed against a Brahma reference sequence ( ) using CpG Viewer (Leeds Institute of Molecular Medicine, Leeds, England).

    Ethanol Precipitation:

    Article Title: Extracting single genomes from heterogenous DNA samples: A test case with Carsonella ruddii, the bacterial symbiont of psyllids (Insecta)
    Article Snippet: Topoisomerase-mediated cloning Restriction enzyme-digested DNA was purified by phenol extraction and concentrated by ethanol precipitation. .. TOPO cloning reactions were transformed into electrocompetent TOP10 cells (Invitrogen) to achieve a high yield of recombinant clones.

    Ligation:

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: .. Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.). .. Southern and Northern analyses were carried out as described previously ( ).

    Northern Blot:

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.). .. Southern and Northern analyses were carried out as described previously ( ).

    Labeling:

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. DNA from colonies was amplified using M13 primers and labeled using BigDye V3.0 (Applied Biosystems, Foster City, CA).

    Purification:

    Article Title: Extracting single genomes from heterogenous DNA samples: A test case with Carsonella ruddii, the bacterial symbiont of psyllids (Insecta)
    Article Snippet: Topoisomerase-mediated cloning Restriction enzyme-digested DNA was purified by phenol extraction and concentrated by ethanol precipitation. .. TOPO cloning reactions were transformed into electrocompetent TOP10 cells (Invitrogen) to achieve a high yield of recombinant clones.

    Polymerase Chain Reaction:

    Article Title: Extracting single genomes from heterogenous DNA samples: A test case with Carsonella ruddii, the bacterial symbiont of psyllids (Insecta)
    Article Snippet: After heat inactivating the phosphatase at 65 °C for 15 min, cloning was performed with the Zero-Blunt TOPO PCR Cloning Kit (Invitrogen, www.intergenco.com ), using 200 ng of dephosphorylated insert DNA, according to the manufacturer's instructions. .. TOPO cloning reactions were transformed into electrocompetent TOP10 cells (Invitrogen) to achieve a high yield of recombinant clones.

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: .. The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. DNA from colonies was amplified using M13 primers and labeled using BigDye V3.0 (Applied Biosystems, Foster City, CA).

    DNA Sequencing:

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.). .. DNA sequencing was performed by cycle sequencing with fluorescent-labeled dideoxynucleoside triphosphates.

    Sequencing:

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.). .. DNA sequencing was performed by cycle sequencing with fluorescent-labeled dideoxynucleoside triphosphates.

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. The methylation status of CpG sites from each clone was analyzed against a Brahma reference sequence ( ) using CpG Viewer (Leeds Institute of Molecular Medicine, Leeds, England).

    Gel Extraction:

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: Fragments were separated by agarose gel electrophoresis, and DNA was extracted by using a QIAquick gel extraction kit (QIAGEN, Valencia, Calif.). .. Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.).

    Transformation Assay:

    Article Title: Extracting single genomes from heterogenous DNA samples: A test case with Carsonella ruddii, the bacterial symbiont of psyllids (Insecta)
    Article Snippet: .. TOPO cloning reactions were transformed into electrocompetent TOP10 cells (Invitrogen) to achieve a high yield of recombinant clones. .. DNA sequencing and data analysis Plasmid DNA inserts from the TOPO cloning reactions were sequenced at the University of Arizona sequencing facility, using M13 forward and reverse primers.

    Article Title: Mitochondrial DNA Ligases of Trypanosoma brucei
    Article Snippet: .. Transformation of bacterial cells was performed by mixing 1 μl of ligation reaction mixture with 20 μl of electrocompetent TOP10 cells (Invitrogen, Inc.) and then electroporating the cells with a Cell-Porator Escherichia coli electroporator (Gibco BRL, Gaithersburg, Md.). .. Southern and Northern analyses were carried out as described previously ( ).

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: .. The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. DNA from colonies was amplified using M13 primers and labeled using BigDye V3.0 (Applied Biosystems, Foster City, CA).

    Recombinant:

    Article Title: Extracting single genomes from heterogenous DNA samples: A test case with Carsonella ruddii, the bacterial symbiont of psyllids (Insecta)
    Article Snippet: .. TOPO cloning reactions were transformed into electrocompetent TOP10 cells (Invitrogen) to achieve a high yield of recombinant clones. .. DNA sequencing and data analysis Plasmid DNA inserts from the TOPO cloning reactions were sequenced at the University of Arizona sequencing facility, using M13 forward and reverse primers.

    Plasmid Preparation:

    Article Title: Methylation Patterns of Brahma during Spermatogenesis and Oogenesis: Potential Implications
    Article Snippet: .. The PCR fragment ligated with TOPO cloning vector was transformed into electrocompetent TOP10 cells (Invitrogen, Carlsbad, CA) ( ). .. DNA from colonies was amplified using M13 primers and labeled using BigDye V3.0 (Applied Biosystems, Foster City, CA).

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  • 99
    Thermo Fisher top10 electrocompetent cells
    Top10 Electrocompetent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 electrocompetent cells/product/Thermo Fisher
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    top10 electrocompetent cells - by Bioz Stars, 2020-03
    99/100 stars
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