Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: This modified ElectraSense reader has externalized leads on the case, which provide connections between electrodes on the array and a potentiostat or other external power source.

Article Snippet: TMB Conductivity 1 Component HRP Microwell Substrate (BioFX) was added to the array, and it was scanned immediately with an ElectraSense microarray reader (CombiMatrix).

Techniques: Modification

Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: Reagents are loaded in a 96-well plate, and a programming script is used to control their flow into a single chamber or into four chambers containing an ElectraSense microarray. Current or voltage can be applied to single or groups of electrodes for Ppy deposition, and current can be read from a single electrode or groups of electrodes for ECD.

Article Snippet: TMB Conductivity 1 Component HRP Microwell Substrate (BioFX) was added to the array, and it was scanned immediately with an ElectraSense microarray reader (CombiMatrix).

Techniques: Microarray

Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: A) Photomicrograph of an ElectraSense microarray under epi illumination and showing the deposition of polypyrrole with adsorbed murine IgG on a 5×5 set of electrodes. B) Fluorescence scanned image of the microarray treated with Cy5-labeled goat anti mouse IgG and showing the presence of murine Ab on the electrodes.

Article Snippet: TMB Conductivity 1 Component HRP Microwell Substrate (BioFX) was added to the array, and it was scanned immediately with an ElectraSense microarray reader (CombiMatrix).

Techniques: Microarray, Fluorescence, Labeling

Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: A ) Three concentrations (0.003, 0.01, and 0.03 pg/ml) were incubated on a microarray with either anti-SEB MAb (•-•) or casein (▪-▪) adsorbed onto Ppy that was deposited at 40 nA for 1 s. SEB binding was detected using biotinylated rabbit anti-SEB Ab and SA-HRP. Background (0 pg/ml SEB) was 1147±283. B ) Different concentrations of SEB were incubated on a microtiter plate with anti-SEB MAb adsorbed onto the surface of each well. SEB binding was detected using biotinylated rabbit anti-SEB Ab and SA-HRP. Background (0 pg/ml SEB) was OD 450–570 2.939±.026.

Article Snippet: TMB Conductivity 1 Component HRP Microwell Substrate (BioFX) was added to the array, and it was scanned immediately with an ElectraSense microarray reader (CombiMatrix).

Techniques: Incubation, Microarray, Binding Assay