Review





Similar Products

materials ell culture ek293 cells  (ATCC)
99
ATCC materials ell culture ek293 cells
Materials Ell Culture Ek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/materials ell culture ek293 cells/product/ATCC
Average 99 stars, based on 1 article reviews
materials ell culture ek293 cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
mouse vegf elisa kits  (Multi Sciences (Lianke) Biotech Co Ltd)
94
Multi Sciences (Lianke) Biotech Co Ltd mouse vegf elisa kits
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Mouse Vegf Elisa Kits, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse vegf elisa kits/product/Multi Sciences (Lianke) Biotech Co Ltd
Average 94 stars, based on 1 article reviews
mouse vegf elisa kits - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
recombinant ek293 cell line  (BPS Bioscience)
94
BPS Bioscience recombinant ek293 cell line
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Recombinant Ek293 Cell Line, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant ek293 cell line/product/BPS Bioscience
Average 94 stars, based on 1 article reviews
recombinant ek293 cell line - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
ek293  (Multi Sciences (Lianke) Biotech Co Ltd)
94
Multi Sciences (Lianke) Biotech Co Ltd ek293
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Ek293, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ek293/product/Multi Sciences (Lianke) Biotech Co Ltd
Average 94 stars, based on 1 article reviews
ek293 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
egf  (Multi Sciences (Lianke) Biotech Co Ltd)
94
Multi Sciences (Lianke) Biotech Co Ltd egf
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Egf, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egf/product/Multi Sciences (Lianke) Biotech Co Ltd
Average 94 stars, based on 1 article reviews
egf - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
dna transfection ek293 cells  (ATCC)
99
ATCC dna transfection ek293 cells
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Dna Transfection Ek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna transfection ek293 cells/product/ATCC
Average 99 stars, based on 1 article reviews
dna transfection ek293 cells - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
mi 100 ek293 derived mouse ifn beta protein r d systems  (R&D Systems)
97
R&D Systems mi 100 ek293 derived mouse ifn beta protein r d systems
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Mi 100 Ek293 Derived Mouse Ifn Beta Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mi 100 ek293 derived mouse ifn beta protein r d systems/product/R&D Systems
Average 97 stars, based on 1 article reviews
mi 100 ek293 derived mouse ifn beta protein r d systems - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
ek293 (human embryonic kidney) cell lines  (National Centre for Cell Science)
90
National Centre for Cell Science ek293 (human embryonic kidney) cell lines
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Ek293 (Human Embryonic Kidney) Cell Lines, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ek293 (human embryonic kidney) cell lines/product/National Centre for Cell Science
Average 90 stars, based on 1 article reviews
ek293 (human embryonic kidney) cell lines - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
ek283 ek293  (Multi Sciences (Lianke) Biotech Co Ltd)
93
Multi Sciences (Lianke) Biotech Co Ltd ek283 ek293
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Ek283 Ek293, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ek283 ek293/product/Multi Sciences (Lianke) Biotech Co Ltd
Average 93 stars, based on 1 article reviews
ek283 ek293 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
template h ek293  (ATCC)
99
ATCC template h ek293
Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) <t>ELISA</t> for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001
Template H Ek293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/template h ek293/product/ATCC
Average 99 stars, based on 1 article reviews
template h ek293 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

Image Search Results


Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) ELISA for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Molecular Medicine

Article Title: Metformin ameliorates osteoporosis by enhancing bone angiogenesis via the YAP1/TAZ-HIF1α axis

doi: 10.1186/s10020-025-01169-7

Figure Lengend Snippet: Metformin promotes type H vessel formation in OVX mice and elevates endothelial angiogenesis in vitro under hypoxic conditions. ( A-B ) Representative Ki67 and EMCN co-immunostaining images ( A ) with quantification of the number of Ki67 positive endothelial cells (Ki67 + ECs, B ). gp: growth plate. tb: trabecular bone. bm: bone marrow. Scale bar: 200 μm. n = 4 per group. ( C-D ) Representative CD31 and EMCN co-immunostaining images ( C ) with quantification of the ratio of CD31 hi EMCN hi vessel ( D ) in femoral metaphysis from Sham, Sham + Metf, OVX and OVX + Metf groups. Scale bar: 200 μm. n = 4–5 per group. ( E ) ELISA for the serum and bone marrow concentrations of VEGFA. n = 4–5 per group; ( F ) CCK-8 analysis for the proliferation of metformin-treated HMECs under different oxygen concentrations (1% and 21% O 2 ). n = 4 per group; ( G-H ) Representative images ( G ) of tube formation of HMECs on Matrigel with quantification of the total tube length, total loops and total branching points ( H ) in (G). Scale bar: 100 μm; n = 3 per group. Data are plotted as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: According to the manufacturer’s instructions, the concentrations of VEGFA were determined by commercial Mouse VEGF ELISA Kits (Multi Sciences LTD., Hangzhou, China).

Techniques: In Vitro, Immunostaining, Enzyme-linked Immunosorbent Assay, CCK-8 Assay