Egta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Effects of extracellular Ca2+ concentration on hair-bundle stiffness and gating-spring integrity in hair cells"
Article Title: Effects of extracellular Ca2+ concentration on hair-bundle stiffness and gating-spring integrity in hair cells
Journal: Proceedings of the National Academy of Sciences of the United States of America
Figure Legend Snippet: Receptor-potential assays of tip-link integrity. ( A ) The top trace represents the 100-nm, 120-ms displacement pulse delivered to the base of a fiber of stiffness 184 μN⋅m − 1 . The bundle’s mechanical response is shown in the middle trace; the cell’s receptor potential is displayed in the bottom trace. The preparation was bathed in standard saline solution containing 4 mM Ca 2+ . Note the twitch in the mechanical record and the corresponding depolarizing spike in the receptor-potential record. The bundle’s average instantaneous stiffness was 611 μN⋅m − 1 . ( B ) Comparable measurements were taken from a hair cell in the sacculus contralateral to that of A , which had been incubated for 15 min in NACSS that had been passed over a BAPTA–polystyrene column to remove Ca 2+ . The bundle produced a twitch and the cell displayed a healthy receptor potential in spite of the prior exposure to a Ca 2+ concentration of only ≈300 nM. The bundle’s average instantaneous stiffness was 724 μN⋅m − 1 ; the stimulus fiber and pulse size used were identical to those for A . Receptor potentials indicative of sensitive transduction were recorded from an additional 14 cells exposed to BAPTA–polystyrene-treated NACSS. ( C ) Receptor potentials were eliminated after a 5-min exposure to a solution containing 5 mM EGTA. This treatment also reduced the bundle’s stiffness to 81 μN⋅m − 1 . Transduction was eliminated and bundle stiffness reduced in each of 8 other cells treated with 5 mM EGTA; similar results were obtained for each of 29 cells treated with 5 mM BAPTA for 1–10 min. The bundle was displaced with a 200-nm stimulus of the same duration as in A and B ; the probe’s stiffness was 446 μN⋅m − 1 . The 100-nm displacement calibration applies only to the middle trace of C .
Techniques Used: Mass Spectrometry, Incubation, Produced, Concentration Assay, Transduction
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