egta  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher egta
    Receptor-potential assays of tip-link integrity. ( A ) The top trace represents the 100-nm, 120-ms displacement pulse delivered to the base of a fiber of stiffness 184 μN⋅m − 1 . The bundle’s mechanical response is shown in the middle trace; the cell’s receptor potential is displayed in the bottom trace. The preparation was bathed in standard saline solution containing 4 mM Ca 2+ . Note the twitch in the mechanical record and the corresponding depolarizing spike in the receptor-potential record. The bundle’s average instantaneous stiffness was 611 μN⋅m − 1 . ( B ) Comparable measurements were taken from a hair cell in the sacculus contralateral to that of A , which had been incubated for 15 min in NACSS that had been passed over a <t>BAPTA–polystyrene</t> column to remove Ca 2+ . The bundle produced a twitch and the cell displayed a healthy receptor potential in spite of the prior exposure to a Ca 2+ concentration of only ≈300 nM. The bundle’s average instantaneous stiffness was 724 μN⋅m − 1 ; the stimulus fiber and pulse size used were identical to those for A . Receptor potentials indicative of sensitive transduction were recorded from an additional 14 cells exposed to BAPTA–polystyrene-treated NACSS. ( C ) Receptor potentials were eliminated after a 5-min exposure to a solution containing 5 mM <t>EGTA.</t> This treatment also reduced the bundle’s stiffness to 81 μN⋅m − 1 . Transduction was eliminated and bundle stiffness reduced in each of 8 other cells treated with 5 mM EGTA; similar results were obtained for each of 29 cells treated with 5 mM BAPTA for 1–10 min. The bundle was displaced with a 200-nm stimulus of the same duration as in A and B ; the probe’s stiffness was 446 μN⋅m − 1 . The 100-nm displacement calibration applies only to the middle trace of C .
    Egta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Effects of extracellular Ca2+ concentration on hair-bundle stiffness and gating-spring integrity in hair cells"

    Article Title: Effects of extracellular Ca2+ concentration on hair-bundle stiffness and gating-spring integrity in hair cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    Receptor-potential assays of tip-link integrity. ( A ) The top trace represents the 100-nm, 120-ms displacement pulse delivered to the base of a fiber of stiffness 184 μN⋅m − 1 . The bundle’s mechanical response is shown in the middle trace; the cell’s receptor potential is displayed in the bottom trace. The preparation was bathed in standard saline solution containing 4 mM Ca 2+ . Note the twitch in the mechanical record and the corresponding depolarizing spike in the receptor-potential record. The bundle’s average instantaneous stiffness was 611 μN⋅m − 1 . ( B ) Comparable measurements were taken from a hair cell in the sacculus contralateral to that of A , which had been incubated for 15 min in NACSS that had been passed over a BAPTA–polystyrene column to remove Ca 2+ . The bundle produced a twitch and the cell displayed a healthy receptor potential in spite of the prior exposure to a Ca 2+ concentration of only ≈300 nM. The bundle’s average instantaneous stiffness was 724 μN⋅m − 1 ; the stimulus fiber and pulse size used were identical to those for A . Receptor potentials indicative of sensitive transduction were recorded from an additional 14 cells exposed to BAPTA–polystyrene-treated NACSS. ( C ) Receptor potentials were eliminated after a 5-min exposure to a solution containing 5 mM EGTA. This treatment also reduced the bundle’s stiffness to 81 μN⋅m − 1 . Transduction was eliminated and bundle stiffness reduced in each of 8 other cells treated with 5 mM EGTA; similar results were obtained for each of 29 cells treated with 5 mM BAPTA for 1–10 min. The bundle was displaced with a 200-nm stimulus of the same duration as in A and B ; the probe’s stiffness was 446 μN⋅m − 1 . The 100-nm displacement calibration applies only to the middle trace of C .
    Figure Legend Snippet: Receptor-potential assays of tip-link integrity. ( A ) The top trace represents the 100-nm, 120-ms displacement pulse delivered to the base of a fiber of stiffness 184 μN⋅m − 1 . The bundle’s mechanical response is shown in the middle trace; the cell’s receptor potential is displayed in the bottom trace. The preparation was bathed in standard saline solution containing 4 mM Ca 2+ . Note the twitch in the mechanical record and the corresponding depolarizing spike in the receptor-potential record. The bundle’s average instantaneous stiffness was 611 μN⋅m − 1 . ( B ) Comparable measurements were taken from a hair cell in the sacculus contralateral to that of A , which had been incubated for 15 min in NACSS that had been passed over a BAPTA–polystyrene column to remove Ca 2+ . The bundle produced a twitch and the cell displayed a healthy receptor potential in spite of the prior exposure to a Ca 2+ concentration of only ≈300 nM. The bundle’s average instantaneous stiffness was 724 μN⋅m − 1 ; the stimulus fiber and pulse size used were identical to those for A . Receptor potentials indicative of sensitive transduction were recorded from an additional 14 cells exposed to BAPTA–polystyrene-treated NACSS. ( C ) Receptor potentials were eliminated after a 5-min exposure to a solution containing 5 mM EGTA. This treatment also reduced the bundle’s stiffness to 81 μN⋅m − 1 . Transduction was eliminated and bundle stiffness reduced in each of 8 other cells treated with 5 mM EGTA; similar results were obtained for each of 29 cells treated with 5 mM BAPTA for 1–10 min. The bundle was displaced with a 200-nm stimulus of the same duration as in A and B ; the probe’s stiffness was 446 μN⋅m − 1 . The 100-nm displacement calibration applies only to the middle trace of C .

    Techniques Used: Mass Spectrometry, Incubation, Produced, Concentration Assay, Transduction

    Related Articles

    other:

    Article Title: Presynaptic inhibition of spontaneous acetylcholine release induced by adenosine at the mouse neuromuscular junction
    Article Snippet: In order to decrease [Ca2+ ]i , nerve terminals were loaded with EGTA-AM (Molecular Probes), a cell-permeant buffer, as follows.

    Article Title: L1-dependent neuritogenesis involves ankyrinB that mediates L1-CAM coupling with retrograde actin flow
    Article Snippet: In brief, DRGs were treated with 0.25% trypsin-EGTA (Life Technologies) at 37°C for 30 min, and triturated using the p20 micropipetman (∼150 strokes) in 20 μl of 10 μM Alexa® 594 phalloidin in Leibovitz's L-15 medium.

    Article Title: The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro
    Article Snippet: After confluence, HBDCs were detached using collagenase and trypsin/EGTA (Gibco) treatment.

    Article Title: Parallel Mechanisms Encode Direction in the Retina
    Article Snippet: Voltage-clamp whole-cell recordings were made using 4–6 MΩ electrodes containing: 112.5 mM CsCH3 SO3 , 9.7 mM KCl, 1 mM MgCl2 , 1.5 mM EGTA, 10 mM HEPES, 4 mM ATP Mg2 , 0.5 mM GTP Na3 , and 0.2 mM Alexa 594 (Invitrogen, Burlington, Ontario, Canada).

    Article Title: Satellite Cells and Markers of Muscle Regeneration during Unloading and Reloading: Effects of Treatment with Resveratrol and Curcumin
    Article Snippet: The resultant pellets, which contained the nuclei, were resuspended in 200 µL of extraction buffer containing 50 mM HEPES potassium hydroxide (HEPES KOH, pH 7.5), 420 mM NaCl, 0.5 mM EDTA, 0.1 mM Ethylene glycol tetraacetic acid (EGTA) and 10% glycerol.

    Article Title: A Novel Type of Neuron Within the Dorsal Striatum
    Article Snippet: Recording pipettes (6–8 MΩ resistance) were filled with an internal solution containing (in mM, unless otherwise stated): 130 potassium gluconate, 10 KOH, 2.5 MgCl2 , 10 HEPES, 4 Na2 ATP, 0.4 Na3 GTP, 5 EGTA, 5 disodium phosphocreatine, 0.025 Alexa fluor 594 (Invitrogen) and 0.2% (w/v) neurobiotin (Vector Labs).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher egta am
    (A) Effect of calcium chelators and K channel antagonist, ChTX on fEPSP during OGD. 1μM <t>BAPTA-AM</t> increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). Similarly, the amplitude of fEPSPs remaining after 2 min, 4 min, 6 min of OGD (n = 6) is increased after administration of 10 nM ChTX. Combining ChTX and BAPTA-AM led to almost no change in fEPSP amplitude up to 6 min of OGD (n = 6). ( B) Effect of calcium chelators, and BK channel antagonist on recovery of fEPSP after OGD. BAPTA-AM (1 μM) decreases recovery time from 2 min (n = 7), 4 min (n = 6) and 6 min (n = 3) of in vitro OGD compared to control (n = 7, 5, 5, respectively). <t>EGTA-AM</t> (50μM) shows similar effects to BAPTA-AM (1μM) by decreasing time needed for electrophysiological recovery after 4 min of OGD (n = 6) but not after 6 min (n = 7). ChTX (10 nM) did not significantly decrease recovery time after 6 min of ischemia (n = 4) but a combination of BAPTA-AM (1 μM) and ChTX(10 nM) significantly decreased recovery time after 6 min of OGD (n = 6). ( C) BAPTA-AM and BAPTA-AM + ChTX promote increased tissue resistance to a long ischemic episode. BAPTA-AM (1 μM) increases the amount of fEPSP remaining after 8 min of OGD and leads to full recovery after 40 min of reperfusion post ischemia (n = 3) when control tissue has surpassed the point of functional recovery (n = 4). A combination of BAPTA-AM (1 μM) and ChTX (10 nM) leads to almost no change in fEPSP amplitude after prolonged OGD and leads to almost full recovery after 40 min of reperfusion (n = 5). Data plotted as mean ± SE. * p
    Egta Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta am/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta am - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

    93
    Thermo Fisher np egta
    Release of <t>ATP</t> is unaltered in dnSNARE mice. (a) ATP could be detected in a concentration‐dependent manner based on resorufin fluorescence in the modified enzymatic assay as determined by cell‐free measurements on a microplate reader. No or significantly smaller signals were detected in the absence of ATP or ATP‐specific enzymes (EC = enzyme control). Values represent mean ± SEM from 4 independent experiments. (b) Increase in ATP release following UV‐induced photolysis of <t>NP‐EGTA</t> in Müller cells from wildtype (Wt, n = 7 cells) and dnSNARE mice ( n = 7 cells). No ATP signal was observed after the removal of the ATP‐specific enzymes. Insets: micrographs showing resorufin fluorescence above a Müller cell endfoot (demarcated by dashed line) before and after the UV pulse. (c) Calcium‐induced ATP release in Müller cells from wildtype (Wt) and dnSNARE mice. No signal was detected in the absence of NP‐EGTA or ATP‐specific enzymes (EC = enzyme control) ( n = 7–11 cells from 2 animals of each genotype). Compared with measurements in the absence of NP‐EGTA, but with all enzymes included: *** p
    Np Egta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/np egta/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    np egta - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) Effect of calcium chelators and K channel antagonist, ChTX on fEPSP during OGD. 1μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). Similarly, the amplitude of fEPSPs remaining after 2 min, 4 min, 6 min of OGD (n = 6) is increased after administration of 10 nM ChTX. Combining ChTX and BAPTA-AM led to almost no change in fEPSP amplitude up to 6 min of OGD (n = 6). ( B) Effect of calcium chelators, and BK channel antagonist on recovery of fEPSP after OGD. BAPTA-AM (1 μM) decreases recovery time from 2 min (n = 7), 4 min (n = 6) and 6 min (n = 3) of in vitro OGD compared to control (n = 7, 5, 5, respectively). EGTA-AM (50μM) shows similar effects to BAPTA-AM (1μM) by decreasing time needed for electrophysiological recovery after 4 min of OGD (n = 6) but not after 6 min (n = 7). ChTX (10 nM) did not significantly decrease recovery time after 6 min of ischemia (n = 4) but a combination of BAPTA-AM (1 μM) and ChTX(10 nM) significantly decreased recovery time after 6 min of OGD (n = 6). ( C) BAPTA-AM and BAPTA-AM + ChTX promote increased tissue resistance to a long ischemic episode. BAPTA-AM (1 μM) increases the amount of fEPSP remaining after 8 min of OGD and leads to full recovery after 40 min of reperfusion post ischemia (n = 3) when control tissue has surpassed the point of functional recovery (n = 4). A combination of BAPTA-AM (1 μM) and ChTX (10 nM) leads to almost no change in fEPSP amplitude after prolonged OGD and leads to almost full recovery after 40 min of reperfusion (n = 5). Data plotted as mean ± SE. * p

    Journal: PLoS ONE

    Article Title: Raised Intracellular Calcium Contributes to Ischemia-Induced Depression of Evoked Synaptic Transmission

    doi: 10.1371/journal.pone.0148110

    Figure Lengend Snippet: (A) Effect of calcium chelators and K channel antagonist, ChTX on fEPSP during OGD. 1μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). Similarly, the amplitude of fEPSPs remaining after 2 min, 4 min, 6 min of OGD (n = 6) is increased after administration of 10 nM ChTX. Combining ChTX and BAPTA-AM led to almost no change in fEPSP amplitude up to 6 min of OGD (n = 6). ( B) Effect of calcium chelators, and BK channel antagonist on recovery of fEPSP after OGD. BAPTA-AM (1 μM) decreases recovery time from 2 min (n = 7), 4 min (n = 6) and 6 min (n = 3) of in vitro OGD compared to control (n = 7, 5, 5, respectively). EGTA-AM (50μM) shows similar effects to BAPTA-AM (1μM) by decreasing time needed for electrophysiological recovery after 4 min of OGD (n = 6) but not after 6 min (n = 7). ChTX (10 nM) did not significantly decrease recovery time after 6 min of ischemia (n = 4) but a combination of BAPTA-AM (1 μM) and ChTX(10 nM) significantly decreased recovery time after 6 min of OGD (n = 6). ( C) BAPTA-AM and BAPTA-AM + ChTX promote increased tissue resistance to a long ischemic episode. BAPTA-AM (1 μM) increases the amount of fEPSP remaining after 8 min of OGD and leads to full recovery after 40 min of reperfusion post ischemia (n = 3) when control tissue has surpassed the point of functional recovery (n = 4). A combination of BAPTA-AM (1 μM) and ChTX (10 nM) leads to almost no change in fEPSP amplitude after prolonged OGD and leads to almost full recovery after 40 min of reperfusion (n = 5). Data plotted as mean ± SE. * p

    Article Snippet: Bis-(o -aminophenoxy)ethane-N ,N ,N ′,N ′-tetraacetic acid acetoxymethyl ester (BAPTA-AM) and EGTA-AM (Molecular Probes, Eugene, OR) were initially dissolved in DMSO, and then diluted to their final concentrations in the ACSF.

    Techniques: In Vitro, Functional Assay

    Cell-permeant calcium chelators reduce ischemia-induced depression of fEPSP amplitudes. (A) Time course of the depression and subsequent recovery of fEPSP amplitudes in drug (left) and control (right) condition. Left Calcium chelator data. 4min OGD in the presence EGTA-AM (grey triangle, n = 6) or BAPTA-AM (black circle, n = 6) leads to a smaller depression of fEPSP amplitudes relative to control, along with faster recovery of the response (approximately 5–6 min). Right Control data. 4min of OGD produces a large depression of fEPSP amplitude, which then takes approximately 9 min to recover (n = 5). (B) Amount of fEPSP amplitude remaining after oxygen-glucose deprivation. 1 μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). 50 μM EGTA-AM (n = 6) shows similar effects to BAPTA-AM (1 μM) at 4 min of OGD, with both chelators increasing the fEPSP amplitude remaining after OGD. At 6 min however, EGTA-AM does not significantly reduce OGD-induced depression of fEPSP amplitude relative to control (n = 6) Data plotted as mean ± SE. * p

    Journal: PLoS ONE

    Article Title: Raised Intracellular Calcium Contributes to Ischemia-Induced Depression of Evoked Synaptic Transmission

    doi: 10.1371/journal.pone.0148110

    Figure Lengend Snippet: Cell-permeant calcium chelators reduce ischemia-induced depression of fEPSP amplitudes. (A) Time course of the depression and subsequent recovery of fEPSP amplitudes in drug (left) and control (right) condition. Left Calcium chelator data. 4min OGD in the presence EGTA-AM (grey triangle, n = 6) or BAPTA-AM (black circle, n = 6) leads to a smaller depression of fEPSP amplitudes relative to control, along with faster recovery of the response (approximately 5–6 min). Right Control data. 4min of OGD produces a large depression of fEPSP amplitude, which then takes approximately 9 min to recover (n = 5). (B) Amount of fEPSP amplitude remaining after oxygen-glucose deprivation. 1 μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). 50 μM EGTA-AM (n = 6) shows similar effects to BAPTA-AM (1 μM) at 4 min of OGD, with both chelators increasing the fEPSP amplitude remaining after OGD. At 6 min however, EGTA-AM does not significantly reduce OGD-induced depression of fEPSP amplitude relative to control (n = 6) Data plotted as mean ± SE. * p

    Article Snippet: Bis-(o -aminophenoxy)ethane-N ,N ,N ′,N ′-tetraacetic acid acetoxymethyl ester (BAPTA-AM) and EGTA-AM (Molecular Probes, Eugene, OR) were initially dissolved in DMSO, and then diluted to their final concentrations in the ACSF.

    Techniques:

    Filamin A is locally translated in injured axons. A , C , E , and G , Western blots of control (− Ax ) and axotomized (+ Ax ) sciatic nerves treated with vehicle or cycloheximide ( CHX , A ), EGTA ( C ), Gö6976 ( E ), and H-89 ( G ). B , D , F , and H , quantifications of filamin A levels in A , C , E , and G . Data are mean ± S.E. n = 3; ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Filamin A Is Required in Injured Axons for HDAC5 Activity and Axon Regeneration *

    doi: 10.1074/jbc.M115.638445

    Figure Lengend Snippet: Filamin A is locally translated in injured axons. A , C , E , and G , Western blots of control (− Ax ) and axotomized (+ Ax ) sciatic nerves treated with vehicle or cycloheximide ( CHX , A ), EGTA ( C ), Gö6976 ( E ), and H-89 ( G ). B , D , F , and H , quantifications of filamin A levels in A , C , E , and G . Data are mean ± S.E. n = 3; ***, p

    Article Snippet: For sciatic nerve delivery of chemical compounds, the chemicals cycloheximide (1 mg/kg, Sigma), EGTA (10 m m , Life Technologies), Gö6976 (1 mg/kg, Tocris), and H-89 (1 mg/kg, Tocris) were dissolved in dimethyl sulfoxide.

    Techniques: Western Blot

    Release of ATP is unaltered in dnSNARE mice. (a) ATP could be detected in a concentration‐dependent manner based on resorufin fluorescence in the modified enzymatic assay as determined by cell‐free measurements on a microplate reader. No or significantly smaller signals were detected in the absence of ATP or ATP‐specific enzymes (EC = enzyme control). Values represent mean ± SEM from 4 independent experiments. (b) Increase in ATP release following UV‐induced photolysis of NP‐EGTA in Müller cells from wildtype (Wt, n = 7 cells) and dnSNARE mice ( n = 7 cells). No ATP signal was observed after the removal of the ATP‐specific enzymes. Insets: micrographs showing resorufin fluorescence above a Müller cell endfoot (demarcated by dashed line) before and after the UV pulse. (c) Calcium‐induced ATP release in Müller cells from wildtype (Wt) and dnSNARE mice. No signal was detected in the absence of NP‐EGTA or ATP‐specific enzymes (EC = enzyme control) ( n = 7–11 cells from 2 animals of each genotype). Compared with measurements in the absence of NP‐EGTA, but with all enzymes included: *** p

    Journal: Glia

    Article Title: Suppression of SNARE‐dependent exocytosis in retinal glial cells and its effect on ischemia‐induced neurodegeneration, et al. Suppression of SNARE‐dependent exocytosis in retinal glial cells and its effect on ischemia‐induced neurodegeneration

    doi: 10.1002/glia.23144

    Figure Lengend Snippet: Release of ATP is unaltered in dnSNARE mice. (a) ATP could be detected in a concentration‐dependent manner based on resorufin fluorescence in the modified enzymatic assay as determined by cell‐free measurements on a microplate reader. No or significantly smaller signals were detected in the absence of ATP or ATP‐specific enzymes (EC = enzyme control). Values represent mean ± SEM from 4 independent experiments. (b) Increase in ATP release following UV‐induced photolysis of NP‐EGTA in Müller cells from wildtype (Wt, n = 7 cells) and dnSNARE mice ( n = 7 cells). No ATP signal was observed after the removal of the ATP‐specific enzymes. Insets: micrographs showing resorufin fluorescence above a Müller cell endfoot (demarcated by dashed line) before and after the UV pulse. (c) Calcium‐induced ATP release in Müller cells from wildtype (Wt) and dnSNARE mice. No signal was detected in the absence of NP‐EGTA or ATP‐specific enzymes (EC = enzyme control) ( n = 7–11 cells from 2 animals of each genotype). Compared with measurements in the absence of NP‐EGTA, but with all enzymes included: *** p

    Article Snippet: Chloromethyl‐tetramethyl‐rosamine (Mitotracker Orange), NP‐EGTA (o‐nitrophenyl EGTA), NPE‐ATP (P(3)‐[1‐(2‐nitrophenyl)]ethyl ester of ATP), and Fluo‐4 AM were from Molecular Probes (Life Technologies, Carlsbad, CA).

    Techniques: Mouse Assay, Concentration Assay, Fluorescence, Modification, Enzymatic Assay