Egta, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 86 stars, based on 1 article reviews
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1) Product Images from "Role of Calmodulin-Calmodulin Kinase II, cAMP/Protein Kinase A and ERK 1/2 on Aeromonas hydrophila-Induced Apoptosis of Head Kidney Macrophages"
Article Title: Role of Calmodulin-Calmodulin Kinase II, cAMP/Protein Kinase A and ERK 1/2 on Aeromonas hydrophila-Induced Apoptosis of Head Kidney Macrophages
Journal: PLoS Pathogens
Figure Legend Snippet: A. hydrophila infection leads to increased CaM expression in HKM. (A) HKM transfected separately with CaM-siRNA, scrambled siRNA or pre-treated with CMZ, Vp, Nf, EGTA, BAPTA/AM for different time periods were infected with A. hydrophila and CaM protein content measured in the lysates 2 h p.i. using EIA kit. HKM pre-treated with CMZ or transfected with CaM-siRNA or scrambled siRNA were infected with A. hydrophila and checked for (B) Hoechst 33342 positive cells and caspase-3 activity and (C) AV-PI staining 24 h p.i. (D) HKM transfected with CaM-siRNA or scrambled siRNA were infected with A. hydrophila and CaM mRNA expression detected by real time PCR 2 h p.i. Vertical bars represent mean ± SE (n = 6). * P
Techniques Used: Infection, Chick Chorioallantoic Membrane Assay, Expressing, Transfection, Enzyme-linked Immunosorbent Assay, Activity Assay, Staining, Real-time Polymerase Chain Reaction
2) Product Images from "Herpes simplex virus triggers activation of calcium-signaling pathways"
Article Title: Herpes simplex virus triggers activation of calcium-signaling pathways
Journal: The Journal of Cell Biology
Figure Legend Snippet: 2-APB and BAPTA-AM prevent viral-induced FAK phosphorylation. CaSki cells were serum starved overnight, and a synchronized infection with HSV-2(G) was conducted as described in Materials and methods. 100 μM 2-APB, 10 μM nifedipine, or control buffer (5% DMSO) was added to cells at the time of binding or penetration (temperature shift, 15 min, 37°C). Cells were treated with low pH citrate buffer, washed, and cell lysates were prepared 10 min after citrate treatment. Western blots were prepared, probed with anti-FAK pY 397 , and scanned by optical densitometry. Blots were then stripped and reprobed with mAb to total FAK. A representative blot is shown in A. Alternatively, cells were pretreated for 2 h with BAPTA-AM, EGTA, or 5% methanol in PBS (control), and then exposed to HSV-2(G). (B) Lysates were prepared 5 min after infection and analyzed by Western blotting for FAK phosphorylation. (C) Results obtained from three independent experiments are summarized graphically as odu of phosphorylated FAK:odu total FAK as a percentage of control (HSV-2(G) in the absence of any inhibitors). Results are the mean ± SD obtained from three independent experiments; asterisks indicate P
Techniques Used: Infection, Binding Assay, Western Blot
Figure Legend Snippet: The ER Ca 2+ release is required for HSV infection. The effects of pretreating cells for 1 h with 50 μM BAPTA-AM, 0.5 mM EGTA, or adding 100 μM 2-APB, 10 μM nifedipine, or 10 μM verapamil during viral penetration on HSV-1(KOS), HSV-2(G), or VSV infection of Vero cells (top) or CaSki cells (bottom) were examined. Results are presented as pfu formed in the presence of the indicated concentration of drug as a percentage of pfu formed in cells treated with control buffer (5% DMSO for pharmacological inhibitors or 5% methanol for BAPTA), and are means of at least three independent experiments conducted in duplicate. Cytotoxicity of pharmacological agents was determined in parallel after a 2-h exposure to drug and quantifying viable, proliferating cells at 24 h by MTS assay. Cell viability results are expressed as odu reading in the presence of the drug as a percentage of odu reading in the presence of control buffer, and are means of two independent experiments conducted in triplicate. Asterisks denote P
Techniques Used: Infection, Concentration Assay, MTS Assay
3) Product Images from "Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3?: An Implication for Cytopathic Effects in Betel Quid Chewers"
Article Title: Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3?: An Implication for Cytopathic Effects in Betel Quid Chewers
Journal: PLoS ONE
Figure Legend Snippet: Calcium signaling was involved in ANE-induced pyknotic necrosis. (A) Calcium flux in SAS cells treated with the indicated doses of ANE was measured as described in experimental procedures. (B) Similar to the condition in (A), calcium flux was evaluated in the presence or absence of NAC. (C) Cells co-treated with ANE and either 5 mM EGTA or 5 µM BAPTA-AM were photographed at the indicated time points.
Article Title: Spontaneous miniature outward currents in mechanically dissociated rat Meynert neurons
Article Snippet: However, the SMOC amplitude was never increased by applying EGTA-AM or BAPTA-AM ( ).
Article Title: HSP70 Acetylation Prevents Combined mTORC1/2 Inhibitor and Curcumin Treatment-Induced Apoptosis
Article Title: Myosin V functions as a vesicle tether at the plasma membrane to control neurotransmitter release in central synapses
Article Snippet: Pharmacology MyoVin-1 (Millipore), Pentabromopseudalin (PBP, Fisher Scientific) or EGTA-AM (Millipore) were diluted in
Article Title: Activity-Dependent Maintenance of Long-Term Potentiation at Visual Cortical Inhibitory Synapses
Article Snippet: The compounds used were obtained from the following sources: APV, DNQX, and from Tocris Cookson (Bristol, UK); iberiotoxin, tetrodotoxin (TTX),
Article Title: Salt-Induced Expression of the Vacuolar H+-ATPase in the Common Ice Plant Is Developmentally Controlled and Tissue Specific 1
Article Snippet: The solution was supplemented with 400 m m NaCl or 400 m m NaCl and effectors, i.e. 20 m m EGTA/800 μ m EGTA/AM (Calbiochem, La Jolla, CA), 50 μ m neomycin sulfate (Calbiochem), 400 μ m
Article Title: Calbindin-D28K Limits Dopamine Release in Ventral but Not Dorsal Striatum by Regulating Ca2+ Availability and Dopamine Transporter Function
Article Snippet: Drugs and Solutions BAPTA-AM and DHβE were purchased from Ascent Scientific or Tocris UK; pluronic acid was from Life Technologies;
Article Title: Characterizing the Site and Mode of Action of Dynorphin at Hippocampal Mossy Fiber Synapses in the Guinea Pig
Article Snippet: .. Although
Article Title: Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKK?
Article Snippet: Accordingly, incubation of L6 myotubes with BMT-17 significantly increased the AMPK activity. .. Pre-incubation with