Structured Review

Fisher Scientific egta
Egta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egta/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
egta - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Transferring:

Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Because this assay does not need biochemical quantities of purified MAPs, we believe that it represents a significant advantage over the traditional microtubule co-sedimentation assay. .. Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, ¾ in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) THE™ alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss Immersol™ 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see ) Blocking buffer (see ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391)

Microscopy:

Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Because this assay does not need biochemical quantities of purified MAPs, we believe that it represents a significant advantage over the traditional microtubule co-sedimentation assay. .. Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, ¾ in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) THE™ alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss Immersol™ 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see ) Blocking buffer (see ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391)

Labeling:

Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Because this assay does not need biochemical quantities of purified MAPs, we believe that it represents a significant advantage over the traditional microtubule co-sedimentation assay. .. Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, ¾ in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) THE™ alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss Immersol™ 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see ) Blocking buffer (see ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391)

Purification:

Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Because this assay does not need biochemical quantities of purified MAPs, we believe that it represents a significant advantage over the traditional microtubule co-sedimentation assay. .. Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, ¾ in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) THE™ alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss Immersol™ 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see ) Blocking buffer (see ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391)

End-sequence Profiling:

Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Because this assay does not need biochemical quantities of purified MAPs, we believe that it represents a significant advantage over the traditional microtubule co-sedimentation assay. .. Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, ¾ in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) THE™ alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss Immersol™ 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see ) Blocking buffer (see ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391)

Blocking Assay:

Article Title: An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Article Snippet: Because this assay does not need biochemical quantities of purified MAPs, we believe that it represents a significant advantage over the traditional microtubule co-sedimentation assay. .. Avant 1.7 ml microcentrifuge tubes (MIDSCI, catalog number: AVSS1700) Pipette tips, 20 μl (Rainin Instrument, LLC, catalog number: 17005091) Pipette tips, 250 μl (Rainin Instrument, LLC, catalog number: 17005093) Razor blades (Fisher Scientific, catalog number: 12640) Microcentrifuge tube rack (Fisher Scientific, catalog number: 22–313630) Scotch double-sided tape, ¾ in. x 400 in. (3M) Microscope slides, 3 in. x 1 in. (Fisher Scientific, catalog number: 12518100A3) Cover glass, No. 1.5, 30 mm x 22 mm (Fisher Scientific, catalog number: 12544A) Filter forcep (EMD Millipore, catalog number: XX6200006P) Kimwipe (Fisher Scientific, catalog number: 06666A) Microscope lens paper, 4 in. x 6 in. sheets (Fisher Scientific, catalog number: 11996) Unlabeled porcine tubulin, > 99% pure, 1 mg (Cytoskeleton, Inc., catalog number: T240) (store at −80 °C) HiLyte Fluor 488 labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL488M) (store at −80 °C) Rhodamine labeled porcine tubulin, 20 μg (Cytoskeleton, Inc., catalog number: TL590M) (store at −80 °C) 100 mM GTP (Cytoskeleton, Inc., catalog number: BST06–010) (store at −20 °C) 10 mM Taxol (Cytoskeleton, Inc., catalog number: TXD01) (store at −20 °C) Precision Red Advanced Protein Assay Reagent (Cytoskeleton, Inc., catalog number: ADV02) (store at room temperature) PIPES (Sigma-Aldrich, catalog number: P1851) (store at room temperature) EGTA (Fisher Scientific, catalog number: O2783–100) (store at room temperature) Magnesium Chloride Hexahydrate (Fisher Scientific, catalog number: BP214–500) Ultrapure water from Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) THE™ alpha Tubulin Antibody, mAb, mouse (GenScript, catalog number: A01410) (store at −20 °C) Pluronic F-127, 100 g (Anatrace, catalog number: P305) (store at room temperature) Carl Zeiss Immersol™ 518 F Immersion Oil (Fisher Scientific, catalog number: 1262466A) (store at room temperature) PEM Buffer (see ) Blocking buffer (see ) .. Ice bucket (Fisher Scientific, catalog number: 07210123) Milli-Q® Advantage A10 Water Purification System (Millipore Sigma) Sorvall centrifuge equipped with S120-AT2 rotor (Thermo Fisher Scientific, model: Discovery) 37 °C incubator (Fisher Scientific, catalog number: 11690525D) Microcentrifuge (Eppendorf, model: 5424) Inverted fluorescence microscope equipped with Perfect Focus System and a 100×/1.49 NA objective (Nikon, model: Ti-E) Laser launch system with 405/488/561/640 nm wavelengths at 15 mW per line (Nikon, model: LUN4) Electron multiplying CCD camera (Andor Technology, model: iXon 888) GFP filter cube set for imaging HiLyte Fluor 488 or Alexa Fluor 488 fluorescence (Chroma Technology Corp., catalog number: 49002) TRITC filter cube set for imaging rhodamine or TMR fluorescence (Chroma Technology Corp., catalog number: 49008) HP workstation with 3.5 GHz 6 core Xeon processor, 64 GB memory, 1 TB hard drive, and 64bit Windows 7 (Micro Video Instruments, Inc., catalog number: 99909) Pipet-Lite LTS pipette L-20XLS+ (Rainin Instrument, LLC, catalog number: 17014392) Pipet-Lite LTS pipette L-200XLS+ (Rainin Instrument, LLC, catalog number: 17014391)

other:

Article Title: Nitric Oxide–Dependent Feedback Loop Regulates Transient Receptor Potential Vanilloid 4 ( TRPV4) Channel Cooperativity and Endothelial Function in Small Pulmonary Arteries
Article Snippet: DAF‐FM diacetate, Fluo‐4AM (Ca2+ indicator), and EGTA‐AM (Ca2+ chelator) were purchased from Fischer Scientific (Pittsburgh, PA).

Injection:

Article Title: Neurons Lacking Huntingtin Differentially Colonize Brain and Survive in Chimeric Mice
Article Snippet: Each of these brains was collected as 6–12 separate series in 0.1 m PB and 0.02% sodium azide and stored until processed for histochemistry, histology, or immunohistochemistry. .. For four additional ES blastocyst injection chimeras the brains were removed and immersion fixed overnight in 0.2% paraformaldehyde in 0.1 m PIPES, pH 6.9, plus 2 m m MgCl2 and 5 m m EGTA, washed in PBS and MgCl2 , cryo-preserved in 30% sucrose plus MgCl2 , embedded in Tissue-Tek OCT compound (Fisher Scientific, Pittsburgh, PA), and frozen. ..

Centrifugation:

Article Title: The Localization of the Brain-Specific Inorganic Phosphate Transporter Suggests a Specific Presynaptic Role in Glutamatergic Transmission
Article Snippet: .. After its addition to 0.32 m sucrose and 10 m m HEPES, pH 7.4 [containing 5 m m Mg-EGTA, 0.4 μ m diisopropylfluorophosphate (DFP), and (in μg/ml) 2 aprotinin, 2 E64, 5 leupeptin, and 2 pepstatin], the mixture was homogenized at ∼750 rpm for 12 strokes in a Wheaton glass/Teflon homogenizer (clearance 0.1–0.15 mm; Fisher Scientific), and the cell debris was removed by centrifugation at 1600 × g for 10 min at 4°C. ..

Kinase Assay:

Article Title: Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms
Article Snippet: Stained tissues were examined by confocal microscopy and total number of BrdU positive RTECs were estimated by counting a minimum of 1000 cells per group. .. Kinase assay Renal tissues and cells were lysed with a buffer containing 150 mM NaCl (Fisher Scientific), 1 mM EDTA (Fisher Scientific), 1 mM EGTA (Fisher Scientific), 1% Triton X-100, 2.5 mM sodium pyrophosphate (Sigma), 1 mM β-glycerol phosphate (Sigma), 1 mM Na3 VO4 (Sigma), 10 μg/ml leupeptin (Sigma), 10 μg/ml aprotinin (Sigma), 1 mM phenylmethylsulfonyl fluoride (Sigma), 50 mM NaF (Sigma), 0.2% dodecyl β-d-maltoside (ThermoFisher Scientific), and 20 mM Tris (pH 7.5, Fisher Scientific) as described previously [ ]. .. The soluble extracts were then subjected to CDK4/6 immunoprecipitation.

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  • 86
    Fisher Scientific egta
    Typical force versus relative deformation profiles for <t>MDA-MB-468</t> cells labeled with various dyes: a control cell (1, red), a <t>EGTA</t> treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein
    Egta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific egta am
    NO ‐ GC ‐ PKG signaling impairs Ca 2+ ‐dependent cooperative openings of TRPV 4 channels in small PA s. TRPV 4 sparklets were recorded in PA s loaded with <t>fluo‐4</t> AM (10 μmol/L). The experiments were performed in the presence of CPA (20 μmol/L) to eliminate the interference from Ca 2+ release from intracellular stores. L‐ NNA (100 μmol/L) was used as an inhibitor of NOS and spermine NONO ate ( NONO ate, 10–30 μmol/L) was used as a NO donor. Coupling coefficients or κ values for coupling among TRPV 4 channels were determined using a coupled Markov Chain model in Matlab. Changes in internal diameter were recorded in cannulated fourth‐order PA s pressurized to 15 mm Hg. A, Experiments were performed in the presence of GSK 101 (6 nmol/L) and L‐ NNA (100 μmol/L). Representative F/F 0 traces indicate the effect of membrane‐permeable Ca 2+ chelator <t>EGTA</t> ‐ AM and NONO ate in the presence of EGTA ‐ AM on TRPV 4 sparklet activity. B, Averaged TRPV 4 sparklet activity per site indicating a decrease in sparklet activity with EGTA ‐ AM and lack of effect of NONO ate on TRPV 4 sparklet activity in the presence of EGTA ‐ AM (n=24 sites; P
    Egta Am, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta am/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta am - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific m egta
    NO ‐ GC ‐ PKG signaling impairs Ca 2+ ‐dependent cooperative openings of TRPV 4 channels in small PA s. TRPV 4 sparklets were recorded in PA s loaded with <t>fluo‐4</t> AM (10 μmol/L). The experiments were performed in the presence of CPA (20 μmol/L) to eliminate the interference from Ca 2+ release from intracellular stores. L‐ NNA (100 μmol/L) was used as an inhibitor of NOS and spermine NONO ate ( NONO ate, 10–30 μmol/L) was used as a NO donor. Coupling coefficients or κ values for coupling among TRPV 4 channels were determined using a coupled Markov Chain model in Matlab. Changes in internal diameter were recorded in cannulated fourth‐order PA s pressurized to 15 mm Hg. A, Experiments were performed in the presence of GSK 101 (6 nmol/L) and L‐ NNA (100 μmol/L). Representative F/F 0 traces indicate the effect of membrane‐permeable Ca 2+ chelator <t>EGTA</t> ‐ AM and NONO ate in the presence of EGTA ‐ AM on TRPV 4 sparklet activity. B, Averaged TRPV 4 sparklet activity per site indicating a decrease in sparklet activity with EGTA ‐ AM and lack of effect of NONO ate on TRPV 4 sparklet activity in the presence of EGTA ‐ AM (n=24 sites; P
    M Egta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Typical force versus relative deformation profiles for MDA-MB-468 cells labeled with various dyes: a control cell (1, red), a EGTA treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein

    Journal: The journal of physical chemistry. B

    Article Title: Cell tracing dyes significantly change single cell mechanics

    doi: 10.1021/jp8103358

    Figure Lengend Snippet: Typical force versus relative deformation profiles for MDA-MB-468 cells labeled with various dyes: a control cell (1, red), a EGTA treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein

    Article Snippet: To investigate role of calcium ions in cell stiffening MDA-MB-468 cells were incubated with 5 mM ethylene glycol tetraacetic acid, EGTA (Fisher Scientific, Fairlawn, NJ) for 30 min before compression.

    Techniques: Multiple Displacement Amplification, Labeling

    NO ‐ GC ‐ PKG signaling impairs Ca 2+ ‐dependent cooperative openings of TRPV 4 channels in small PA s. TRPV 4 sparklets were recorded in PA s loaded with fluo‐4 AM (10 μmol/L). The experiments were performed in the presence of CPA (20 μmol/L) to eliminate the interference from Ca 2+ release from intracellular stores. L‐ NNA (100 μmol/L) was used as an inhibitor of NOS and spermine NONO ate ( NONO ate, 10–30 μmol/L) was used as a NO donor. Coupling coefficients or κ values for coupling among TRPV 4 channels were determined using a coupled Markov Chain model in Matlab. Changes in internal diameter were recorded in cannulated fourth‐order PA s pressurized to 15 mm Hg. A, Experiments were performed in the presence of GSK 101 (6 nmol/L) and L‐ NNA (100 μmol/L). Representative F/F 0 traces indicate the effect of membrane‐permeable Ca 2+ chelator EGTA ‐ AM and NONO ate in the presence of EGTA ‐ AM on TRPV 4 sparklet activity. B, Averaged TRPV 4 sparklet activity per site indicating a decrease in sparklet activity with EGTA ‐ AM and lack of effect of NONO ate on TRPV 4 sparklet activity in the presence of EGTA ‐ AM (n=24 sites; P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Nitric Oxide–Dependent Feedback Loop Regulates Transient Receptor Potential Vanilloid 4 ( TRPV4) Channel Cooperativity and Endothelial Function in Small Pulmonary Arteries

    doi: 10.1161/JAHA.117.007157

    Figure Lengend Snippet: NO ‐ GC ‐ PKG signaling impairs Ca 2+ ‐dependent cooperative openings of TRPV 4 channels in small PA s. TRPV 4 sparklets were recorded in PA s loaded with fluo‐4 AM (10 μmol/L). The experiments were performed in the presence of CPA (20 μmol/L) to eliminate the interference from Ca 2+ release from intracellular stores. L‐ NNA (100 μmol/L) was used as an inhibitor of NOS and spermine NONO ate ( NONO ate, 10–30 μmol/L) was used as a NO donor. Coupling coefficients or κ values for coupling among TRPV 4 channels were determined using a coupled Markov Chain model in Matlab. Changes in internal diameter were recorded in cannulated fourth‐order PA s pressurized to 15 mm Hg. A, Experiments were performed in the presence of GSK 101 (6 nmol/L) and L‐ NNA (100 μmol/L). Representative F/F 0 traces indicate the effect of membrane‐permeable Ca 2+ chelator EGTA ‐ AM and NONO ate in the presence of EGTA ‐ AM on TRPV 4 sparklet activity. B, Averaged TRPV 4 sparklet activity per site indicating a decrease in sparklet activity with EGTA ‐ AM and lack of effect of NONO ate on TRPV 4 sparklet activity in the presence of EGTA ‐ AM (n=24 sites; P

    Article Snippet: DAF‐FM diacetate, Fluo‐4AM (Ca2+ indicator), and EGTA‐AM (Ca2+ chelator) were purchased from Fischer Scientific (Pittsburgh, PA).

    Techniques: Activity Assay