egta (Dojindo Labs)
Structured Review

Egta, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egta/product/Dojindo Labs
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions"
Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions
Journal: Scientific Reports
doi: 10.1038/srep08610

Figure Legend Snippet: ATP's antimicrobial effect is attributable to its iron-chelating activity. (a) Blocking effects of MgCl 2 and FeCl 3 on ATP's anti- M. intracellulare antimicrobial activity. (b) Anti- M. intracellulare activities of ATP and various metal-chelating agents (pyrophosphate (PPi), EDTA, EGTA) and blocking effects of MgCl 2 . (c) Anti- S. aureus activities of ATP and various metal-chelating agents. (d) Ferric ion-chelating activities of ATP and EDTA measured by CAS assay. (e) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against M. intracellulare . (f) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against P. aeruginosa . (g) Siderophore production by ATP-resistant E. coli during the course of cultivation. (h) Siderophore production by two ATP-resistant K. pneumoniae strains during 48-h cultivation in the presence of ATP. (i) Siderophore production by ATP-susceptible S. aureus and ATP-resistant E. coli during 24-h cultivation in the presence of ATP (5 mM). (j) Siderophore production by ATP-susceptible and ATP-resistant strains of P. aeruginosa during 24-h cultivation.
Techniques Used: Activity Assay, Blocking Assay
2) Product Images from "Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae"
Article Title: Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae
Journal: Biology Open
doi: 10.1242/bio.036590

Figure Legend Snippet: Effects of Ca 2+ on taxol-induced haptonematal bending. (A) A phase contrast image of a haptophyte treated with taxol (20 μM) in the absence of Ca 2+ (CFSW+EGTA+BAPTA-AM) showing a helical configuration of the haptonema (left). The plot to the right shows the curvature along the haptonema (see Fig. S5 ). The curvature of the haptonema was calculated from the microscopic image, which is a two-dimensionally projected image. The curvature of a helical structure calculated in this way shows oscillation with a repeat distance equivalent to that of the helix, and a shift in the pattern represents propagation of the wave form. Scale bar: 10 µm. (B) Ca 2+ -dependent propagation of a taxol-induced haptonematal helix. The helix is also converted to planar bending with the maximum curvature of bend propagating to the proximal region. Trace images of haptophytes are shown as insets.
Techniques Used:
3) Product Images from "Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae"
Article Title: Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae
Journal: Biology Open
doi: 10.1242/bio.036590

Figure Legend Snippet: Effects of Ca 2+ on taxol-induced haptonematal bending. (A) A phase contrast image of a haptophyte treated with taxol (20 μM) in the absence of Ca 2+ (CFSW+EGTA+BAPTA-AM) showing a helical configuration of the haptonema (left). The plot to the right shows the curvature along the haptonema (see Fig. S5 ). The curvature of the haptonema was calculated from the microscopic image, which is a two-dimensionally projected image. The curvature of a helical structure calculated in this way shows oscillation with a repeat distance equivalent to that of the helix, and a shift in the pattern represents propagation of the wave form. Scale bar: 10 µm. (B) Ca 2+ -dependent propagation of a taxol-induced haptonematal helix. The helix is also converted to planar bending with the maximum curvature of bend propagating to the proximal region. Trace images of haptophytes are shown as insets.
Techniques Used:
4) Product Images from "Specific Activation of the G Protein-coupled Receptor BNGR-A21 by the Neuropeptide Corazonin from the Silkworm, Bombyx mori, Dually Couples to the Gq and Gs Signaling Cascades *"
Article Title: Specific Activation of the G Protein-coupled Receptor BNGR-A21 by the Neuropeptide Corazonin from the Silkworm, Bombyx mori, Dually Couples to the Gq and Gs Signaling Cascades *
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M112.441675

Figure Legend Snippet: Intracellular Ca 2+ influx. HEK293 ( A ) and Sf21 ( B ) cells, respectively, transfected with FLAG-A21 and BmFlag-A21 were measured in response to different concentrations of corazonin peptide using the fluorescent Ca 2+ indicator fura-2. C , effect of pretreatment of the G q inhibitor on corazonin (1 μ m )-mediated Ca 2+ influx in HEK293 cells. D , effects of pretreatment of calcium chelators (EGTA, 5 m m , and BAPTA-AM, 50 μ m ) and l -calcium channel (nefidipine, 10 μ m ) inhibitors on corazonin-mediated Ca 2+ influx in HEK293 cells. Effect of pretreatment of PLC inhibitor (U73122, 10 μ m ) on corazonin-mediated Ca 2+ influx in HEK293 ( E ) and Sf21 ( F ) cells. The figures are representative of more than three independent experiments.
Techniques Used: Transfection, Planar Chromatography
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Polymerase Chain Reaction:Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions Article Snippet: .. Special agents The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), other:Article Title: Mechanisms of the thapsigargin-induced Ca2+ entry in in situ endothelial cells of the porcine aortic valve and the endothelium-dependent relaxation in the porcine coronary artery Article Snippet: Fura-2/AM and Article Title: RNG105 Deficiency Impairs the Dendritic Localization of mRNAs for Na+/K+ ATPase Subunit Isoforms and Leads to the Degeneration of Neuronal Networks Article Snippet: EGTA and Ligation:Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions Article Snippet: .. Special agents The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), |