egta tetra acetoxymethyl ester egta am  (Thermo Fisher)


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    Name:
    EGTA Tetra acetoxymethyl Ester EGTA AM
    Description:
    The cell permeant EGTA AM can be passively loaded into cells to generate intracellular EGTA This compound is intrinsically a liquid or an oil at room temperature
    Catalog Number:
    e1219
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Calcium Detection|Cell Analysis|Ionic Homeostasis & Signaling|Cell Viability, Proliferation & Function
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    Thermo Fisher egta tetra acetoxymethyl ester egta am
    The cell permeant EGTA AM can be passively loaded into cells to generate intracellular EGTA This compound is intrinsically a liquid or an oil at room temperature
    https://www.bioz.com/result/egta tetra acetoxymethyl ester egta am/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta tetra acetoxymethyl ester egta am - by Bioz Stars, 2021-03
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    Article Title: L-Type Ca2+ Channel Sparklets Revealed by TIRF Microscopy in Mouse Urinary Bladder Smooth Muscle
    Article Snippet: All drugs were purchased from Sigma-Aldrich Japan (Tokyo, Japan) except for Oregon Green 488 BAPTA-1 AM, EGTA-AM (Life Technologies, Tokyo, Japan) and R-(+)-Bay K 8644 (Tocris Biosciences, Bristol, UK).

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    Thermo Fisher egta am
    (A) Effect of calcium chelators and K channel antagonist, ChTX on fEPSP during OGD. 1μM <t>BAPTA-AM</t> increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). Similarly, the amplitude of fEPSPs remaining after 2 min, 4 min, 6 min of OGD (n = 6) is increased after administration of 10 nM ChTX. Combining ChTX and BAPTA-AM led to almost no change in fEPSP amplitude up to 6 min of OGD (n = 6). ( B) Effect of calcium chelators, and BK channel antagonist on recovery of fEPSP after OGD. BAPTA-AM (1 μM) decreases recovery time from 2 min (n = 7), 4 min (n = 6) and 6 min (n = 3) of in vitro OGD compared to control (n = 7, 5, 5, respectively). <t>EGTA-AM</t> (50μM) shows similar effects to BAPTA-AM (1μM) by decreasing time needed for electrophysiological recovery after 4 min of OGD (n = 6) but not after 6 min (n = 7). ChTX (10 nM) did not significantly decrease recovery time after 6 min of ischemia (n = 4) but a combination of BAPTA-AM (1 μM) and ChTX(10 nM) significantly decreased recovery time after 6 min of OGD (n = 6). ( C) BAPTA-AM and BAPTA-AM + ChTX promote increased tissue resistance to a long ischemic episode. BAPTA-AM (1 μM) increases the amount of fEPSP remaining after 8 min of OGD and leads to full recovery after 40 min of reperfusion post ischemia (n = 3) when control tissue has surpassed the point of functional recovery (n = 4). A combination of BAPTA-AM (1 μM) and ChTX (10 nM) leads to almost no change in fEPSP amplitude after prolonged OGD and leads to almost full recovery after 40 min of reperfusion (n = 5). Data plotted as mean ± SE. * p
    Egta Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Effect of calcium chelators and K channel antagonist, ChTX on fEPSP during OGD. 1μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). Similarly, the amplitude of fEPSPs remaining after 2 min, 4 min, 6 min of OGD (n = 6) is increased after administration of 10 nM ChTX. Combining ChTX and BAPTA-AM led to almost no change in fEPSP amplitude up to 6 min of OGD (n = 6). ( B) Effect of calcium chelators, and BK channel antagonist on recovery of fEPSP after OGD. BAPTA-AM (1 μM) decreases recovery time from 2 min (n = 7), 4 min (n = 6) and 6 min (n = 3) of in vitro OGD compared to control (n = 7, 5, 5, respectively). EGTA-AM (50μM) shows similar effects to BAPTA-AM (1μM) by decreasing time needed for electrophysiological recovery after 4 min of OGD (n = 6) but not after 6 min (n = 7). ChTX (10 nM) did not significantly decrease recovery time after 6 min of ischemia (n = 4) but a combination of BAPTA-AM (1 μM) and ChTX(10 nM) significantly decreased recovery time after 6 min of OGD (n = 6). ( C) BAPTA-AM and BAPTA-AM + ChTX promote increased tissue resistance to a long ischemic episode. BAPTA-AM (1 μM) increases the amount of fEPSP remaining after 8 min of OGD and leads to full recovery after 40 min of reperfusion post ischemia (n = 3) when control tissue has surpassed the point of functional recovery (n = 4). A combination of BAPTA-AM (1 μM) and ChTX (10 nM) leads to almost no change in fEPSP amplitude after prolonged OGD and leads to almost full recovery after 40 min of reperfusion (n = 5). Data plotted as mean ± SE. * p

    Journal: PLoS ONE

    Article Title: Raised Intracellular Calcium Contributes to Ischemia-Induced Depression of Evoked Synaptic Transmission

    doi: 10.1371/journal.pone.0148110

    Figure Lengend Snippet: (A) Effect of calcium chelators and K channel antagonist, ChTX on fEPSP during OGD. 1μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). Similarly, the amplitude of fEPSPs remaining after 2 min, 4 min, 6 min of OGD (n = 6) is increased after administration of 10 nM ChTX. Combining ChTX and BAPTA-AM led to almost no change in fEPSP amplitude up to 6 min of OGD (n = 6). ( B) Effect of calcium chelators, and BK channel antagonist on recovery of fEPSP after OGD. BAPTA-AM (1 μM) decreases recovery time from 2 min (n = 7), 4 min (n = 6) and 6 min (n = 3) of in vitro OGD compared to control (n = 7, 5, 5, respectively). EGTA-AM (50μM) shows similar effects to BAPTA-AM (1μM) by decreasing time needed for electrophysiological recovery after 4 min of OGD (n = 6) but not after 6 min (n = 7). ChTX (10 nM) did not significantly decrease recovery time after 6 min of ischemia (n = 4) but a combination of BAPTA-AM (1 μM) and ChTX(10 nM) significantly decreased recovery time after 6 min of OGD (n = 6). ( C) BAPTA-AM and BAPTA-AM + ChTX promote increased tissue resistance to a long ischemic episode. BAPTA-AM (1 μM) increases the amount of fEPSP remaining after 8 min of OGD and leads to full recovery after 40 min of reperfusion post ischemia (n = 3) when control tissue has surpassed the point of functional recovery (n = 4). A combination of BAPTA-AM (1 μM) and ChTX (10 nM) leads to almost no change in fEPSP amplitude after prolonged OGD and leads to almost full recovery after 40 min of reperfusion (n = 5). Data plotted as mean ± SE. * p

    Article Snippet: Bis-(o -aminophenoxy)ethane-N ,N ,N ′,N ′-tetraacetic acid acetoxymethyl ester (BAPTA-AM) and EGTA-AM (Molecular Probes, Eugene, OR) were initially dissolved in DMSO, and then diluted to their final concentrations in the ACSF.

    Techniques: In Vitro, Functional Assay

    Cell-permeant calcium chelators reduce ischemia-induced depression of fEPSP amplitudes. (A) Time course of the depression and subsequent recovery of fEPSP amplitudes in drug (left) and control (right) condition. Left Calcium chelator data. 4min OGD in the presence EGTA-AM (grey triangle, n = 6) or BAPTA-AM (black circle, n = 6) leads to a smaller depression of fEPSP amplitudes relative to control, along with faster recovery of the response (approximately 5–6 min). Right Control data. 4min of OGD produces a large depression of fEPSP amplitude, which then takes approximately 9 min to recover (n = 5). (B) Amount of fEPSP amplitude remaining after oxygen-glucose deprivation. 1 μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). 50 μM EGTA-AM (n = 6) shows similar effects to BAPTA-AM (1 μM) at 4 min of OGD, with both chelators increasing the fEPSP amplitude remaining after OGD. At 6 min however, EGTA-AM does not significantly reduce OGD-induced depression of fEPSP amplitude relative to control (n = 6) Data plotted as mean ± SE. * p

    Journal: PLoS ONE

    Article Title: Raised Intracellular Calcium Contributes to Ischemia-Induced Depression of Evoked Synaptic Transmission

    doi: 10.1371/journal.pone.0148110

    Figure Lengend Snippet: Cell-permeant calcium chelators reduce ischemia-induced depression of fEPSP amplitudes. (A) Time course of the depression and subsequent recovery of fEPSP amplitudes in drug (left) and control (right) condition. Left Calcium chelator data. 4min OGD in the presence EGTA-AM (grey triangle, n = 6) or BAPTA-AM (black circle, n = 6) leads to a smaller depression of fEPSP amplitudes relative to control, along with faster recovery of the response (approximately 5–6 min). Right Control data. 4min of OGD produces a large depression of fEPSP amplitude, which then takes approximately 9 min to recover (n = 5). (B) Amount of fEPSP amplitude remaining after oxygen-glucose deprivation. 1 μM BAPTA-AM increases the amount of evoked neurotransmission remaining after 2 min (n = 7), 4 min (n = 6) and 6 min (n = 5) of ischemia relative to control (n = 7, 5, 5, respectively). 50 μM EGTA-AM (n = 6) shows similar effects to BAPTA-AM (1 μM) at 4 min of OGD, with both chelators increasing the fEPSP amplitude remaining after OGD. At 6 min however, EGTA-AM does not significantly reduce OGD-induced depression of fEPSP amplitude relative to control (n = 6) Data plotted as mean ± SE. * p

    Article Snippet: Bis-(o -aminophenoxy)ethane-N ,N ,N ′,N ′-tetraacetic acid acetoxymethyl ester (BAPTA-AM) and EGTA-AM (Molecular Probes, Eugene, OR) were initially dissolved in DMSO, and then diluted to their final concentrations in the ACSF.

    Techniques:

    Filamin A is locally translated in injured axons. A , C , E , and G , Western blots of control (− Ax ) and axotomized (+ Ax ) sciatic nerves treated with vehicle or cycloheximide ( CHX , A ), EGTA ( C ), Gö6976 ( E ), and H-89 ( G ). B , D , F , and H , quantifications of filamin A levels in A , C , E , and G . Data are mean ± S.E. n = 3; ***, p

    Journal: The Journal of Biological Chemistry

    Article Title: Filamin A Is Required in Injured Axons for HDAC5 Activity and Axon Regeneration *

    doi: 10.1074/jbc.M115.638445

    Figure Lengend Snippet: Filamin A is locally translated in injured axons. A , C , E , and G , Western blots of control (− Ax ) and axotomized (+ Ax ) sciatic nerves treated with vehicle or cycloheximide ( CHX , A ), EGTA ( C ), Gö6976 ( E ), and H-89 ( G ). B , D , F , and H , quantifications of filamin A levels in A , C , E , and G . Data are mean ± S.E. n = 3; ***, p

    Article Snippet: For sciatic nerve delivery of chemical compounds, the chemicals cycloheximide (1 mg/kg, Sigma), EGTA (10 m m , Life Technologies), Gö6976 (1 mg/kg, Tocris), and H-89 (1 mg/kg, Tocris) were dissolved in dimethyl sulfoxide.

    Techniques: Western Blot

    Ca 2+ sparklets in DSM cells are confined to the cell membrane. Removal of extracellular Ca 2+ significantly reduced the Ca 2+ sparklet frequency without affecting the amplitude of the few remaining sparklets (A–B). In the presence of 10 mM external Ca 2+ , the amplitude and frequency of the Ca 2+ sparklets remained largely unaffected, compared with controls (C–D). When UBSM strips were co-loaded with the Ca 2+ chelator EGTA-AM (10 μM) together with the Ca 2+ indicator (Oregon Green BAPTA-1-AM), Ca 2+ sparklets could still be detected in the UBSM cells (E–F). Compared with controls, Ca 2+ sparklet amplitude remained unaffected by the presence of EGTA-AM (G), whereas Ca 2+ sparklet peak area was significantly reduced by the presence of EGTA-AM (H–I). The red plot denotes a sparklet under control conditions, and the black plot a sparklet in the presence of EGTA-AM (I). Note that when EGTA-AM was used, it was not possible to use paired controls, for the purpose of statistical comparisons; instead, the “controls” comprise 4 randomly selected control experiments from other datasets. Unpaired statistics were used for statistical comparisons of the data presented in panels E, G and H. An asterisk indicates a P value that is considered statistically significant ( P

    Journal: PLoS ONE

    Article Title: L-Type Ca2+ Channel Sparklets Revealed by TIRF Microscopy in Mouse Urinary Bladder Smooth Muscle

    doi: 10.1371/journal.pone.0093803

    Figure Lengend Snippet: Ca 2+ sparklets in DSM cells are confined to the cell membrane. Removal of extracellular Ca 2+ significantly reduced the Ca 2+ sparklet frequency without affecting the amplitude of the few remaining sparklets (A–B). In the presence of 10 mM external Ca 2+ , the amplitude and frequency of the Ca 2+ sparklets remained largely unaffected, compared with controls (C–D). When UBSM strips were co-loaded with the Ca 2+ chelator EGTA-AM (10 μM) together with the Ca 2+ indicator (Oregon Green BAPTA-1-AM), Ca 2+ sparklets could still be detected in the UBSM cells (E–F). Compared with controls, Ca 2+ sparklet amplitude remained unaffected by the presence of EGTA-AM (G), whereas Ca 2+ sparklet peak area was significantly reduced by the presence of EGTA-AM (H–I). The red plot denotes a sparklet under control conditions, and the black plot a sparklet in the presence of EGTA-AM (I). Note that when EGTA-AM was used, it was not possible to use paired controls, for the purpose of statistical comparisons; instead, the “controls” comprise 4 randomly selected control experiments from other datasets. Unpaired statistics were used for statistical comparisons of the data presented in panels E, G and H. An asterisk indicates a P value that is considered statistically significant ( P

    Article Snippet: All drugs were purchased from Sigma-Aldrich Japan (Tokyo, Japan) except for Oregon Green 488 BAPTA-1 AM, EGTA-AM (Life Technologies, Tokyo, Japan) and R-(+)-Bay K 8644 (Tocris Biosciences, Bristol, UK).

    Techniques:

    Effects of BAPTA on A23187- and thapsigargin-dependent activation of calreticulin promoter. NCB1 cells were loaded with 10 μM BAPTA/AM for 30 min in a media containing different drugs for 16 h and lysed, and reporter gene analysis was carried out as described in Materials and Methods. ( Open bars ) Cells grown and incubated with 7 μM A23187 or 100 nM thapsigargin in DME supplemented with 100 μM EGTA; ( dotted bars ) cells loaded with BAPTA/AM followed by incubation with either A23187 or thapsigargin (control cells were not treated with BAPTA/AM); ( filled bars ) cells loaded with BAPTA/AM and incubated with either A23187 or thapsigargin in Ca 2+ -depleted DME. ( Cross-hatched bars ) Cells loaded with 10 μM EGTA/AM for 30 min followed by 16-h incubation with either A23187 or thaps igargin in DME (control cells were not treated with EGTA/ AM); data are shown as a mean ± SD.

    Journal: The Journal of Cell Biology

    Article Title: Regulation of Calreticulin Gene Expression by Calcium

    doi:

    Figure Lengend Snippet: Effects of BAPTA on A23187- and thapsigargin-dependent activation of calreticulin promoter. NCB1 cells were loaded with 10 μM BAPTA/AM for 30 min in a media containing different drugs for 16 h and lysed, and reporter gene analysis was carried out as described in Materials and Methods. ( Open bars ) Cells grown and incubated with 7 μM A23187 or 100 nM thapsigargin in DME supplemented with 100 μM EGTA; ( dotted bars ) cells loaded with BAPTA/AM followed by incubation with either A23187 or thapsigargin (control cells were not treated with BAPTA/AM); ( filled bars ) cells loaded with BAPTA/AM and incubated with either A23187 or thapsigargin in Ca 2+ -depleted DME. ( Cross-hatched bars ) Cells loaded with 10 μM EGTA/AM for 30 min followed by 16-h incubation with either A23187 or thaps igargin in DME (control cells were not treated with EGTA/ AM); data are shown as a mean ± SD.

    Article Snippet: Stock solutions of A23187, thapsigargin, BAPTA/AM (Molecular Probes, Inc., Eugene, OR), and EGTA/AM (Molecular Probes, Inc.) were prepared in 99.5% dimethyl sulfoxide and were added to the culture medium as specified in the text.

    Techniques: Activation Assay, Incubation

    Effects of bradykinin on activation of calreticulin promoter. NCB1 cells were incubated with either 100 nM thapsigargin ( TG ) or 200 nM bradykinin ( BK ) in Ca 2+ -depleted DME supplemented with EGTA. After 16 h the cells were lysed, and then reporter gene analysis was carried out as described in Materials and Methods. Data are shown as a mean ± SD.

    Journal: The Journal of Cell Biology

    Article Title: Regulation of Calreticulin Gene Expression by Calcium

    doi:

    Figure Lengend Snippet: Effects of bradykinin on activation of calreticulin promoter. NCB1 cells were incubated with either 100 nM thapsigargin ( TG ) or 200 nM bradykinin ( BK ) in Ca 2+ -depleted DME supplemented with EGTA. After 16 h the cells were lysed, and then reporter gene analysis was carried out as described in Materials and Methods. Data are shown as a mean ± SD.

    Article Snippet: Stock solutions of A23187, thapsigargin, BAPTA/AM (Molecular Probes, Inc., Eugene, OR), and EGTA/AM (Molecular Probes, Inc.) were prepared in 99.5% dimethyl sulfoxide and were added to the culture medium as specified in the text.

    Techniques: Activation Assay, Incubation