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Affibody egfr binding
Egfr Binding, supplied by Affibody, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 79 stars, based on 2 article reviews
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egfr binding - by Bioz Stars, 2020-02
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Concentration Assay:

Article Title: Influence of composition of cysteine-containing peptide-based chelators on biodistribution of 99mTc-labeled anti-EGFR affibody molecules
Article Snippet: Since internalization of affibody molecules after binding to EGFR is slow (Tolmachev et al. ; Garousi et al. , ), radiolabeled affibody molecules remain reversibly bound to the surface of hepatocytes and dissociate when blood concentration is decreased because of renal clearance. .. The second mechanism is independent on EGFR-binding, but depends on both charge and charge distribution in the C-terminus of affibody molecules (Garousi et al. ).

Binding Assay:

Article Title: Influence of composition of cysteine-containing peptide-based chelators on biodistribution of 99mTc-labeled anti-EGFR affibody molecules
Article Snippet: Since internalization of affibody molecules after binding to EGFR is slow (Tolmachev et al. ; Garousi et al. , ), radiolabeled affibody molecules remain reversibly bound to the surface of hepatocytes and dissociate when blood concentration is decreased because of renal clearance. .. The second mechanism is independent on EGFR-binding, but depends on both charge and charge distribution in the C-terminus of affibody molecules (Garousi et al. ).

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    Affibody egfr binding affibody molecule
    Specificity of 89 Zr-DFO-ZEGFR:2377 uptake in A431 xenografts and <t>EGFR-expressing</t> organs in mice at 3 h after injection. In the blocked group, receptors were saturated by pre-injection of large excess of non-labelled <t>affibody</t> molecules.
    Egfr Binding Affibody Molecule, supplied by Affibody, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr binding affibody molecule/product/Affibody
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    egfr binding affibody molecule - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    83
    Affibody compact egfr binding affibody zegfr
    Modular capacity of probes for labeling <t>EGFR</t> on the cell surface. (A) Structures of the fluorogens used. The synthetic and analytical details were shown in Supporting Information or described previously. 26 A431 cell labeled with <t>FAP–affibody</t> fusions and various malachite green derivatives were analyzed by flow cytometry (B) and live-cell fluorescence microscopy (C). 5 × 10 5 /mL of cells were labeled with 250 nM of AFA or F followed by incubation with 100 nM of fluorogens for 5 min. Cells were then either analyzed by flow cytometry for mean fluorescence measurement or cell imaging. Scale bar 20 μm.
    Compact Egfr Binding Affibody Zegfr, supplied by Affibody, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/compact egfr binding affibody zegfr/product/Affibody
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    compact egfr binding affibody zegfr - by Bioz Stars, 2020-02
    83/100 stars
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    78
    Affibody egfr binding fibronectin
    The effect of washing on enrichment ratio and recovery of yeast displaying <t>fibronectin</t> domain ligands panned on <t>EGFR</t> mid cells Yeast displaying E6.2.6′, E6.2.6′ N78S and WT′ (affinities indicated) mixed 1:1,000 with non-displaying yeast were panned against MDA-MB-231. The enrichment (A) and yield (B) of binding ligands is presented as the mean ± standard deviation of 3–9 replicates.
    Egfr Binding Fibronectin, supplied by Affibody, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr binding fibronectin/product/Affibody
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Specificity of 89 Zr-DFO-ZEGFR:2377 uptake in A431 xenografts and EGFR-expressing organs in mice at 3 h after injection. In the blocked group, receptors were saturated by pre-injection of large excess of non-labelled affibody molecules.

    Journal: International Journal of Oncology

    Article Title: PET imaging of epidermal growth factor receptor expression in tumours using 89Zr-labelled ZEGFR:2377 affibody molecules

    doi: 10.3892/ijo.2016.3369

    Figure Lengend Snippet: Specificity of 89 Zr-DFO-ZEGFR:2377 uptake in A431 xenografts and EGFR-expressing organs in mice at 3 h after injection. In the blocked group, receptors were saturated by pre-injection of large excess of non-labelled affibody molecules.

    Article Snippet: In the present study, an EGFR-binding affibody molecule (ZEGFR:2377) was site-specifically conjugated with a deferoxamine (DFO) chelator and labelled under mild conditions (room temperature and neutral pH) with a positron-emitting radionuclide 89 Zr.

    Techniques: Expressing, Mouse Assay, Injection

    Specific binding and uptake of IRDye800CW-labeled Affibody molecules. (A) The protein expression levels of EGFR and HER2 in MDA-MB-231 (MDA231), A431, SKOV3, and SKBR3 cells. Actin served as an internal control. The relative expression levels were calculated

    Journal: Neoplasia (New York, N.Y.)

    Article Title: In Vivo Imaging of Xenograft Tumors Using an Epidermal Growth Factor Receptor-Specific Affibody Molecule Labeled with a Near-infrared Fluorophore 1

    doi:

    Figure Lengend Snippet: Specific binding and uptake of IRDye800CW-labeled Affibody molecules. (A) The protein expression levels of EGFR and HER2 in MDA-MB-231 (MDA231), A431, SKOV3, and SKBR3 cells. Actin served as an internal control. The relative expression levels were calculated

    Article Snippet: Cellular studies of binding, internalization and retention of a radiolabeled EGFR-binding Affibody molecule.

    Techniques: Binding Assay, Labeling, Expressing, Multiple Displacement Amplification

    The effect of EGF and EGFR-specific Affibody (Eaff) on EGFR-mediated phosphorylation of EGFR and ERK1/2 (P44/42 MAPK) proteins. A431 cells were treated with either Eaff or EGF. Two concentrations (5 and 20 nM) for both Eaff (Eaff5 and Eaff20) and EGF

    Journal: Neoplasia (New York, N.Y.)

    Article Title: In Vivo Imaging of Xenograft Tumors Using an Epidermal Growth Factor Receptor-Specific Affibody Molecule Labeled with a Near-infrared Fluorophore 1

    doi:

    Figure Lengend Snippet: The effect of EGF and EGFR-specific Affibody (Eaff) on EGFR-mediated phosphorylation of EGFR and ERK1/2 (P44/42 MAPK) proteins. A431 cells were treated with either Eaff or EGF. Two concentrations (5 and 20 nM) for both Eaff (Eaff5 and Eaff20) and EGF

    Article Snippet: Cellular studies of binding, internalization and retention of a radiolabeled EGFR-binding Affibody molecule.

    Techniques:

    Modular capacity of probes for labeling EGFR on the cell surface. (A) Structures of the fluorogens used. The synthetic and analytical details were shown in Supporting Information or described previously. 26 A431 cell labeled with FAP–affibody fusions and various malachite green derivatives were analyzed by flow cytometry (B) and live-cell fluorescence microscopy (C). 5 × 10 5 /mL of cells were labeled with 250 nM of AFA or F followed by incubation with 100 nM of fluorogens for 5 min. Cells were then either analyzed by flow cytometry for mean fluorescence measurement or cell imaging. Scale bar 20 μm.

    Journal: Bioconjugate Chemistry

    Article Title: Fluorogen Activating Protein–Affibody Probes: Modular, No-Wash Measurement of Epidermal Growth Factor Receptors

    doi: 10.1021/bc500525b

    Figure Lengend Snippet: Modular capacity of probes for labeling EGFR on the cell surface. (A) Structures of the fluorogens used. The synthetic and analytical details were shown in Supporting Information or described previously. 26 A431 cell labeled with FAP–affibody fusions and various malachite green derivatives were analyzed by flow cytometry (B) and live-cell fluorescence microscopy (C). 5 × 10 5 /mL of cells were labeled with 250 nM of AFA or F followed by incubation with 100 nM of fluorogens for 5 min. Cells were then either analyzed by flow cytometry for mean fluorescence measurement or cell imaging. Scale bar 20 μm.

    Article Snippet: A protein domain (FAP dL5** ) that binds to malachite-green (MG) derivatives for fluorescence activation was expressed as a recombinant fusion to one or two copies of the compact EGFR binding affibody ZEGFR:1907 .

    Techniques: Labeling, Flow Cytometry, Cytometry, Fluorescence, Microscopy, Incubation, Imaging

    Characterization of probes binding on A431 cell surface. (A) Dissociation constant analysis of probes on cell surface. 5 × 10 5 /mL quantities of cells were incubated with probes for 1 h at 37 °C followed by 2 μM MG-B-tau for 5 min. Then cells were kept on ice for flow cytometry. The mean fluorescence intensity was corrected with background of cells incubating with F/MG and then normalized to mean fluorescence at 250 nM of probes. (B) Competition assay of nonfluorescent affibody A binding to the cell surface. Cells were labeled with 250 nM AFA or F and a serial dilution of A followed by 100 nM of MG-B-tau added prior to measurement. (C) Detection of receptor activation by Western blots. Starved cells were labeled with 250 nM probes followed by 100 nM of MG-B-tau and then cells were treated with 100 ng/mL EGF. Then cells were lysed for Western blot in order to detect phosphorylated EGFR and total EGFR. (D) Live-cell fluorescence microscopy of A431 cells labeled by various probes. Cells were labeled with 250 nM of probe and 100 nM of MG-B-tau prior to imaging or 100 nM of Cy5 conjugated affibody dimer. Scale bar 20 μm.

    Journal: Bioconjugate Chemistry

    Article Title: Fluorogen Activating Protein–Affibody Probes: Modular, No-Wash Measurement of Epidermal Growth Factor Receptors

    doi: 10.1021/bc500525b

    Figure Lengend Snippet: Characterization of probes binding on A431 cell surface. (A) Dissociation constant analysis of probes on cell surface. 5 × 10 5 /mL quantities of cells were incubated with probes for 1 h at 37 °C followed by 2 μM MG-B-tau for 5 min. Then cells were kept on ice for flow cytometry. The mean fluorescence intensity was corrected with background of cells incubating with F/MG and then normalized to mean fluorescence at 250 nM of probes. (B) Competition assay of nonfluorescent affibody A binding to the cell surface. Cells were labeled with 250 nM AFA or F and a serial dilution of A followed by 100 nM of MG-B-tau added prior to measurement. (C) Detection of receptor activation by Western blots. Starved cells were labeled with 250 nM probes followed by 100 nM of MG-B-tau and then cells were treated with 100 ng/mL EGF. Then cells were lysed for Western blot in order to detect phosphorylated EGFR and total EGFR. (D) Live-cell fluorescence microscopy of A431 cells labeled by various probes. Cells were labeled with 250 nM of probe and 100 nM of MG-B-tau prior to imaging or 100 nM of Cy5 conjugated affibody dimer. Scale bar 20 μm.

    Article Snippet: A protein domain (FAP dL5** ) that binds to malachite-green (MG) derivatives for fluorescence activation was expressed as a recombinant fusion to one or two copies of the compact EGFR binding affibody ZEGFR:1907 .

    Techniques: Binding Assay, Incubation, Flow Cytometry, Cytometry, Fluorescence, Competitive Binding Assay, Labeling, Serial Dilution, Activation Assay, Western Blot, Microscopy, Imaging

    The effect of washing on enrichment ratio and recovery of yeast displaying fibronectin domain ligands panned on EGFR mid cells Yeast displaying E6.2.6′, E6.2.6′ N78S and WT′ (affinities indicated) mixed 1:1,000 with non-displaying yeast were panned against MDA-MB-231. The enrichment (A) and yield (B) of binding ligands is presented as the mean ± standard deviation of 3–9 replicates.

    Journal: Biotechnology and bioengineering

    Article Title: Geometry and expression enhance enrichment of functional yeast-displayed ligands via cell panning

    doi: 10.1002/bit.26001

    Figure Lengend Snippet: The effect of washing on enrichment ratio and recovery of yeast displaying fibronectin domain ligands panned on EGFR mid cells Yeast displaying E6.2.6′, E6.2.6′ N78S and WT′ (affinities indicated) mixed 1:1,000 with non-displaying yeast were panned against MDA-MB-231. The enrichment (A) and yield (B) of binding ligands is presented as the mean ± standard deviation of 3–9 replicates.

    Article Snippet: EGFR-binding fibronectin clone E6.2.6′, affibody clone EA68, and Gp2 clone GαEGFR2.2.3 were tested.

    Techniques: Multiple Displacement Amplification, Binding Assay, Standard Deviation

    The effect of washing and incubation conditions on enrichment ratio and recovery of yeast displaying fibronectin domain ligands panned on EGFR high cells Yeast displaying E6.2.6′, E6.2.6′ N78S, and WT′ (affinities indicated) mixed 1:1,000 with non-displaying yeast were panned against EGFR-expressing MDA-MB-468. The enrichment and yield of binding ligands is presented as the mean ± standard deviation of 3–9 replicates. (A and B) Selections were performed under baseline conditions with the exception of varied number of washing steps. (C and D) Selections were performed with the indicated modulation of incubation conditions.

    Journal: Biotechnology and bioengineering

    Article Title: Geometry and expression enhance enrichment of functional yeast-displayed ligands via cell panning

    doi: 10.1002/bit.26001

    Figure Lengend Snippet: The effect of washing and incubation conditions on enrichment ratio and recovery of yeast displaying fibronectin domain ligands panned on EGFR high cells Yeast displaying E6.2.6′, E6.2.6′ N78S, and WT′ (affinities indicated) mixed 1:1,000 with non-displaying yeast were panned against EGFR-expressing MDA-MB-468. The enrichment and yield of binding ligands is presented as the mean ± standard deviation of 3–9 replicates. (A and B) Selections were performed under baseline conditions with the exception of varied number of washing steps. (C and D) Selections were performed with the indicated modulation of incubation conditions.

    Article Snippet: EGFR-binding fibronectin clone E6.2.6′, affibody clone EA68, and Gp2 clone GαEGFR2.2.3 were tested.

    Techniques: Incubation, Expressing, Multiple Displacement Amplification, Binding Assay, Standard Deviation