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TaKaRa egfp fused kctd5 encoding plasmid
Retention of <t>KCTD5</t> in the Ni 2+ -NTA column during H. pylori infection. (A) HEK293T and (B) AGS cells were transfected with plasmids encoding <t>EGFP,</t> EGFP-KCTD5 and His-Ubi, lysates from these cells were processed for pull-down with Ni 2+ -NTA column, and the presence of KCTD5 was determined by immunoblot with antibody to KCTD5 (upper panel) and ubiquitinated proteins were shown as retention control in the column (lower panel). AGS transfected cells were infected by 8 h with H. pylori (MOI = 300).
Egfp Fused Kctd5 Encoding Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egfp fused kctd5 encoding plasmid/product/TaKaRa
Average 91 stars, based on 1 article reviews
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egfp fused kctd5 encoding plasmid - by Bioz Stars, 2020-08
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1) Product Images from "KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence"

Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence

Journal: Frontiers in Cellular and Infection Microbiology

doi: 10.3389/fcimb.2017.00450

Retention of KCTD5 in the Ni 2+ -NTA column during H. pylori infection. (A) HEK293T and (B) AGS cells were transfected with plasmids encoding EGFP, EGFP-KCTD5 and His-Ubi, lysates from these cells were processed for pull-down with Ni 2+ -NTA column, and the presence of KCTD5 was determined by immunoblot with antibody to KCTD5 (upper panel) and ubiquitinated proteins were shown as retention control in the column (lower panel). AGS transfected cells were infected by 8 h with H. pylori (MOI = 300).
Figure Legend Snippet: Retention of KCTD5 in the Ni 2+ -NTA column during H. pylori infection. (A) HEK293T and (B) AGS cells were transfected with plasmids encoding EGFP, EGFP-KCTD5 and His-Ubi, lysates from these cells were processed for pull-down with Ni 2+ -NTA column, and the presence of KCTD5 was determined by immunoblot with antibody to KCTD5 (upper panel) and ubiquitinated proteins were shown as retention control in the column (lower panel). AGS transfected cells were infected by 8 h with H. pylori (MOI = 300).

Techniques Used: Infection, Transfection

Related Articles

Clone Assay:

Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence
Article Snippet: .. EGFP-fused KCTD5 encoding plasmid (pEGFP-C1-KCTD5) was generated by cloning the human KCTD5 cDNA from the pMaxKoz-HA-KCTD5 plasmid (Dementieva et al., ) (kindly donated by Dr. Steve Goldstein) into the XhoI/BamHI sites of the pEGFP-C1 plasmid (Catalog number: 6084, Clontech Laboratories, Mountain View, CA, USA). .. KCTD5 shRNA (shRNA#Scramble and shRNA#KCTD5 ) were obtained from Origene Inc. (Origene, Rockville, MD, USA).

Generated:

Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence
Article Snippet: .. EGFP-fused KCTD5 encoding plasmid (pEGFP-C1-KCTD5) was generated by cloning the human KCTD5 cDNA from the pMaxKoz-HA-KCTD5 plasmid (Dementieva et al., ) (kindly donated by Dr. Steve Goldstein) into the XhoI/BamHI sites of the pEGFP-C1 plasmid (Catalog number: 6084, Clontech Laboratories, Mountain View, CA, USA). .. KCTD5 shRNA (shRNA#Scramble and shRNA#KCTD5 ) were obtained from Origene Inc. (Origene, Rockville, MD, USA).

Plasmid Preparation:

Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence
Article Snippet: .. EGFP-fused KCTD5 encoding plasmid (pEGFP-C1-KCTD5) was generated by cloning the human KCTD5 cDNA from the pMaxKoz-HA-KCTD5 plasmid (Dementieva et al., ) (kindly donated by Dr. Steve Goldstein) into the XhoI/BamHI sites of the pEGFP-C1 plasmid (Catalog number: 6084, Clontech Laboratories, Mountain View, CA, USA). .. KCTD5 shRNA (shRNA#Scramble and shRNA#KCTD5 ) were obtained from Origene Inc. (Origene, Rockville, MD, USA).

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    TaKaRa egfp fused kctd5 encoding plasmid
    Retention of <t>KCTD5</t> in the Ni 2+ -NTA column during H. pylori infection. (A) HEK293T and (B) AGS cells were transfected with plasmids encoding <t>EGFP,</t> EGFP-KCTD5 and His-Ubi, lysates from these cells were processed for pull-down with Ni 2+ -NTA column, and the presence of KCTD5 was determined by immunoblot with antibody to KCTD5 (upper panel) and ubiquitinated proteins were shown as retention control in the column (lower panel). AGS transfected cells were infected by 8 h with H. pylori (MOI = 300).
    Egfp Fused Kctd5 Encoding Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfp fused kctd5 encoding plasmid/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egfp fused kctd5 encoding plasmid - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Retention of KCTD5 in the Ni 2+ -NTA column during H. pylori infection. (A) HEK293T and (B) AGS cells were transfected with plasmids encoding EGFP, EGFP-KCTD5 and His-Ubi, lysates from these cells were processed for pull-down with Ni 2+ -NTA column, and the presence of KCTD5 was determined by immunoblot with antibody to KCTD5 (upper panel) and ubiquitinated proteins were shown as retention control in the column (lower panel). AGS transfected cells were infected by 8 h with H. pylori (MOI = 300).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence

    doi: 10.3389/fcimb.2017.00450

    Figure Lengend Snippet: Retention of KCTD5 in the Ni 2+ -NTA column during H. pylori infection. (A) HEK293T and (B) AGS cells were transfected with plasmids encoding EGFP, EGFP-KCTD5 and His-Ubi, lysates from these cells were processed for pull-down with Ni 2+ -NTA column, and the presence of KCTD5 was determined by immunoblot with antibody to KCTD5 (upper panel) and ubiquitinated proteins were shown as retention control in the column (lower panel). AGS transfected cells were infected by 8 h with H. pylori (MOI = 300).

    Article Snippet: EGFP-fused KCTD5 encoding plasmid (pEGFP-C1-KCTD5) was generated by cloning the human KCTD5 cDNA from the pMaxKoz-HA-KCTD5 plasmid (Dementieva et al., ) (kindly donated by Dr. Steve Goldstein) into the XhoI/BamHI sites of the pEGFP-C1 plasmid (Catalog number: 6084, Clontech Laboratories, Mountain View, CA, USA).

    Techniques: Infection, Transfection