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ef 63  (ATCC)


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    Structured Review

    ATCC ef 63
    Ef 63, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ef+63/pmocysc-pUC18/pmc05880108-103-11-19
    Average 90 stars, based on 2 article reviews
    ef 63 - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation
    Article Snippet: 100 μg proteins from each sample were used for iTRAQ 1 labeling. . iTRAQ Labeling and Sample Cleanup 100 μg of proteins extracted from the E. faecalis isolates 63, 64, 51299, and 29212 were labeled using iTRAQ Reagent 8-plex kit (SCIEX) according to the manufacturer's instructions with slight modifications.

    Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation
    Article Snippet: Protein estimation was carried out using Bradford assay (Bio-Rad).

    Labeling:

    Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation
    Article Snippet: Experimental workflow for iTRAQ labeling and analysis: iTRAQ 8-plex labeling was performed for two sets of biological replicates of the strains Ef 63, Ef 64, ATCC 51299, and ATCC 29212. .. Each strain was labeled as follows for the two biological replicates: Ef 63 (113, 117), Ef 64 (114, 118), ATCC 51299 (115, 119), and ATCC 29212 (116, 121). .. The eluted fractions were pooled into ten fractions and desalted with Sep-Pak® tC18 _Elution Plate (186002318, Waters Corp.) using a vacuum manifold (Millipore) before second dimension reversed-phase chromatography.

    Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation
    Article Snippet: 100 μg proteins from each sample were used for iTRAQ 1 labeling. . iTRAQ Labeling and Sample Cleanup 100 μg of proteins extracted from the E. faecalis isolates 63, 64, 51299, and 29212 were labeled using iTRAQ Reagent 8-plex kit (SCIEX) according to the manufacturer's instructions with slight modifications. .. The first set of biological replicates for E. faecalis isolates Ef 63, Ef 64, ATCC 51299, and ATCC 29212 was labeled with labeling reagents 113, 114, 115, and 116, respectively. .. The second set of biological replicates for E. faecalis isolates Ef 63, Ef 64, ATCC 51299, and ATCC 29212 was labeled with labeling reagents 117, 118, 119, and 121, respectively.

    Expressing:

    Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation
    Article Snippet: The differential expression analysis of the proteome was performed by comparing the strong and the weak biofilm-forming E. faecalis isolates Ef 63 and Ef 64 against each of the ATCC strains individually. .. Analysis of fold changes of Ef 63 versus ATCC 51299 (113:115, 113:119, 117:115, 117:119), Ef 63 versus ATCC 29212 (113:116, 113: 121, 117:116, 117:121), Ef 64 versus ATCC 51299 (114:115, 114:119, 118:115, 118:119), and Ef 64 versus ATCC 29212 (114:116, 114:121, 118:116, 118:121) was carried out to get the differential expression profiles. .. Proteins downregulated in 63 Accession # Protein names Gene names Unused protein score Peptides (95%) Fold change wrt 29212 Fold change wrt 51299 UPI000005C830 Dps family protein; DNA protection during starvation protein dps; DR75_1942 25.85 74 0.158975959 0.141553384 UPI0001B1DD9C Galactose mutarotase -aldose epimerase conserved domain galM 19.17 13 0.649019509 0.549455743 UPI0000059961 Replication-associated protein RepB; cobQ/CobB/MinD/ParA nucleotide binding domain protein (plasmid) DR75_2966 10.19 7 0.505973488 0.505026395 UPI00019C6B36 PTS system ascorbate-specific IIB component; PTS system, lactose/cellobiose specific IIB subunit DR75_1696 8 8 0.242902381 0.518277773 UPI000005C334 Aspartate 1-decarboxylase (EC4.1.1.11) (aspartate alpha-decarboxylase) panD; DR75_827 6.65 9 0.405554528 0.274218075 UPI000005C853 7,8-dihydroneopterin aldolase (EC4.1.2.25); dihydroneopterin aldolase folB; DR75_1970 2.47 2 0.631259615 0.438989396 UPI0000059960 Replication-associated protein B; putative replication-associated protein (plasmid) DR75_2967 2.08 1 0.388184377 0.292597088 UPI000005C269 Ethanolamine two-component response regulator; ANTAR domain protein DR75_634 2 1 0.546653837 0.60537555 UPI0001B2588A Glycerophosphoryl diester phosphodiesterase family protein DR75_1000 2 1 0.57470862 0.297523575 UPI000005BCA4 Serine O-acetyltransferase cysE; DR75_2093 2 1 0.399293872 0.34637114 Open in a separate window Functional classification of the significantly down-regulated proteins of Ef 63 in comparison with both ATCC 29212 and 51299 strains meeting both p value cutoff and log 2 fold-change cutoff criteria . (d) Down-regulated Proteins in the Weak Biofilm Former Ef 64 The proteins that are down-regulated in the weak biofilm former Ef 64 are listed in .



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    ef 63  (ATCC)
    90
    ATCC ef 63
    Ef 63, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ef+63/pmocysc-pUC18/pmc05880108-103-11-19
    Average 90 stars, based on 1 article reviews
    ef 63 - by Bioz Stars, 2026-10
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