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eescs  (ATCC)
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ATCC eescs
Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal <t>cells</t> <t>(NESCs)</t> and ectopic endometrial stromal cells <t>(EESCs).</t> Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.
Eescs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eescs  (Dojindo Labs)
99
Dojindo Labs eescs
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
Eescs, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eescs  (MedChemExpress)
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MedChemExpress eescs
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
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ln nag lap 2 eesc  (Vector Laboratories)
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Vector Laboratories ln nag lap 2 eesc
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
Ln Nag Lap 2 Eesc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eescs  (Bio-Rad)
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Bio-Rad eescs
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
Eescs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eesc  (Makino Inc)
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Makino Inc eesc
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
Eesc, supplied by Makino Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eescs  (Thermo Fisher)
99
Thermo Fisher eescs
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
Eescs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary eescs  (Informa UK Limited)
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Informa UK Limited primary eescs
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
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expression and emotion scale for children (eesc)  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology expression and emotion scale for children (eesc)
Figure 3. The effects of IGF2BP1 silencing on <t>eESCs.</t> A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The <t>cell</t> <t>viability</t> of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.
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Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

Journal: Human Mutation

Article Title: Single‐Cell Transcriptomic Profiling and Machine Learning Integration Unveil Stromal Cell Heterogeneity in Endometriosis

doi: 10.1155/humu/5565366

Figure Lengend Snippet: Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

Article Snippet: NESCs (ATCC Cat# CRL‐4003, RRID:CVCL_D697) and EESCs (ATCC Cat# CRL‐7566, RRID:CVCL_IW41) were authenticated by short tandem repeat (STR) profiling, showing ≥ 95% match to the reference profile in the ATCC database.

Techniques: Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Gene Expression

Figure 3. The effects of IGF2BP1 silencing on eESCs. A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The cell viability of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.

Journal: Folia histochemica et cytobiologica

Article Title: Inhibition of IGF2BP1 attenuates the progression of endometriosis through PTBP1.

doi: 10.5603/fhc.98213

Figure Lengend Snippet: Figure 3. The effects of IGF2BP1 silencing on eESCs. A. The obser- vation of eESCs under an inverted microscope. B. The expressions of biomarkers of eESCs were detected by flow cytometry (FCM). C. The mRNA levels of IGF2BP1 in eESCs. D. The cell viability of eESCs was determined. E. The cell proliferation of eESCs was detected. F. The eESC migration and invasion were detected. G. FCM was conducted to identify the apoptotic eESC population. H. The expressions of PCNA, VEGF, and E-cadherin in eESCs were assessed. n = 3 per group. *P < 0.05 vs. si-NC group.

Article Snippet: To assess the viability of eESCs, a cell counting kit-8 (CCK-8, NU679, Dojindo, Kumamoto, Japan) assay was conducted. eESCs were seeded at 5 × 103/well in 96-well plates.

Techniques: Inverted Microscopy, Flow Cytometry, Migration

Figure 4. The effects of IGF2BP1/PTBP1 on eESCs. A. The mRNA levels of IGF2BP1 and PTBP1 in eESCs. B. The cell viability of eESCs was evaluated. C. The proliferation was detected using an EDU assay. D. eESC migration and invasion were detected using Transwell assay. E. Flow cytometry was conducted to identify the apoptotic eESC population. F. The expression levels of PCNA, VEGF, and E-cadherin in eESCs were assessed as described in Methods. n = 3 per group. *P < 0.05 vs. si-IGF2BP1+ +oe-NC group.

Journal: Folia histochemica et cytobiologica

Article Title: Inhibition of IGF2BP1 attenuates the progression of endometriosis through PTBP1.

doi: 10.5603/fhc.98213

Figure Lengend Snippet: Figure 4. The effects of IGF2BP1/PTBP1 on eESCs. A. The mRNA levels of IGF2BP1 and PTBP1 in eESCs. B. The cell viability of eESCs was evaluated. C. The proliferation was detected using an EDU assay. D. eESC migration and invasion were detected using Transwell assay. E. Flow cytometry was conducted to identify the apoptotic eESC population. F. The expression levels of PCNA, VEGF, and E-cadherin in eESCs were assessed as described in Methods. n = 3 per group. *P < 0.05 vs. si-IGF2BP1+ +oe-NC group.

Article Snippet: To assess the viability of eESCs, a cell counting kit-8 (CCK-8, NU679, Dojindo, Kumamoto, Japan) assay was conducted. eESCs were seeded at 5 × 103/well in 96-well plates.

Techniques: EdU Assay, Migration, Transwell Assay, Flow Cytometry, Expressing