Journal: Pathology International

Article Title: High expression of eukaryotic elongation factor 1‐alpha‐2 in lung adenocarcinoma is associated with poor prognosis

doi: 10.1111/pin.13457

Figure Lengend Snippet: (a) Western blot of recombinant protein, protein extracts from the A549 cell line, and from normal human organs. reEF1A1 and reEF1A2 are recombinant proteins. Caption under organ name “1” and “2” mean two cases were examined for heart, lung and liver. (b) Western blot of A549 cells treated with si‐eEF1A2. Scrambled RNA was used for the negative control (NC). LUAD indicates lung adenocarcinoma tissues.

Article Snippet: To verify the specificity of the antibodies against eEF1A1 and eEF1A2, Western blotting was performed using monoclonal anti‐eEF1A1 antibody (ab157455; Abcam), polyclonal anti‐eEF1A2 antibody (GTX102326, GeneTex), and mouse monoclonal anti‐GAPDH antibody (sc‐32233, Santa Cruz Biotechnology). eEF1A1 and eEF1A2 recombinant proteins (Abnova) were used as positive controls.

Techniques: Western Blot, Recombinant, Negative Control

Journal: Pathology International

Article Title: High expression of eukaryotic elongation factor 1‐alpha‐2 in lung adenocarcinoma is associated with poor prognosis

doi: 10.1111/pin.13457

Figure Lengend Snippet: Immunohistochemistry of eukaryotic elongation factor 1 alpha 2 (eEF1A2) in normal lung tissue and in lung adenocarcinoma. (a) Normal lung tissue. (b) Adenocarcinoma in situ. (c) Solid adenocarcinoma with eEF1A2 expression.

Article Snippet: To verify the specificity of the antibodies against eEF1A1 and eEF1A2, Western blotting was performed using monoclonal anti‐eEF1A1 antibody (ab157455; Abcam), polyclonal anti‐eEF1A2 antibody (GTX102326, GeneTex), and mouse monoclonal anti‐GAPDH antibody (sc‐32233, Santa Cruz Biotechnology). eEF1A1 and eEF1A2 recombinant proteins (Abnova) were used as positive controls.

Techniques: Immunohistochemistry, In Situ, Expressing

Journal: Pathology International

Article Title: High expression of eukaryotic elongation factor 1‐alpha‐2 in lung adenocarcinoma is associated with poor prognosis

doi: 10.1111/pin.13457

Figure Lengend Snippet: Kaplan‐Meier curve of disease‐free survival of patients with eukaryotic elongation factor 1 alpha 2 (eEF1A2).

Article Snippet: To verify the specificity of the antibodies against eEF1A1 and eEF1A2, Western blotting was performed using monoclonal anti‐eEF1A1 antibody (ab157455; Abcam), polyclonal anti‐eEF1A2 antibody (GTX102326, GeneTex), and mouse monoclonal anti‐GAPDH antibody (sc‐32233, Santa Cruz Biotechnology). eEF1A1 and eEF1A2 recombinant proteins (Abnova) were used as positive controls.

Techniques:

Journal: Pathology International

Article Title: High expression of eukaryotic elongation factor 1‐alpha‐2 in lung adenocarcinoma is associated with poor prognosis

doi: 10.1111/pin.13457

Figure Lengend Snippet: Univariate and multivariate analysis of adenocarcinoma ( n = 175) using the Cox proportional hazards model.

Article Snippet: To verify the specificity of the antibodies against eEF1A1 and eEF1A2, Western blotting was performed using monoclonal anti‐eEF1A1 antibody (ab157455; Abcam), polyclonal anti‐eEF1A2 antibody (GTX102326, GeneTex), and mouse monoclonal anti‐GAPDH antibody (sc‐32233, Santa Cruz Biotechnology). eEF1A1 and eEF1A2 recombinant proteins (Abnova) were used as positive controls.

Techniques:

Journal: Pathology International

Article Title: High expression of eukaryotic elongation factor 1‐alpha‐2 in lung adenocarcinoma is associated with poor prognosis

doi: 10.1111/pin.13457

Figure Lengend Snippet: (a) Quantitative genomic PCR analysis of eukaryotic elongation factor 1 alpha 2 (eEF1A2) in lung adenocarcinoma. Five tumors immunohistochemically positive for eEF1A2 and two tumors immunohistochemically negative for eEF1A2 were subjected to the analysis. Each dot represents one tumor. A ratio (tumor/normal) of ≥1.5 was defined as representing gene amplification. (b) Fluorescence in situ hybridization of eEF1A2 in lung adenocarcinoma specimen. eEF1A2 amplification was detected in the tumor with increased expression of eEF1A2 DNA. Amplification of eEF1A2 was visualized as an increased number of red signals (eEF1A2) relative to green signals (Chromosome 20 centromeric probe: CEN20p).

Article Snippet: To verify the specificity of the antibodies against eEF1A1 and eEF1A2, Western blotting was performed using monoclonal anti‐eEF1A1 antibody (ab157455; Abcam), polyclonal anti‐eEF1A2 antibody (GTX102326, GeneTex), and mouse monoclonal anti‐GAPDH antibody (sc‐32233, Santa Cruz Biotechnology). eEF1A1 and eEF1A2 recombinant proteins (Abnova) were used as positive controls.

Techniques: Amplification, Fluorescence, In Situ Hybridization, Expressing