edta  (Millipore)

 
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    Name:
    Ethylenediaminetetraacetic dianhydride
    Description:

    Catalog Number:
    332046
    Price:
    None
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    Structured Review

    Millipore edta
    Ethylenediaminetetraacetic dianhydride

    https://www.bioz.com/result/edta/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2021-04
    97/100 stars

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    Related Articles

    Transgenic Assay:

    Article Title: Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk
    Article Snippet: After quantification, only the milk with rhGH > 50 μg/mL was collected from the respective transgenic sows. .. Pretreatment of transgenic sow milk (sample preparation) The transgenic milk samples were diluted with a 2-fold volume of EDTA (Sigma, St. Louis, MO, USA) at a final concentration of 20 mM to extract rhGH within casein micelles. .. Defatted milk samples were acidified by slowly adding 50% acetic acid (Merck Millipore, Billerica, MA, USA) with constant stirring until the milk reached pH 4.25, which precipitated casein.

    Sample Prep:

    Article Title: Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk
    Article Snippet: After quantification, only the milk with rhGH > 50 μg/mL was collected from the respective transgenic sows. .. Pretreatment of transgenic sow milk (sample preparation) The transgenic milk samples were diluted with a 2-fold volume of EDTA (Sigma, St. Louis, MO, USA) at a final concentration of 20 mM to extract rhGH within casein micelles. .. Defatted milk samples were acidified by slowly adding 50% acetic acid (Merck Millipore, Billerica, MA, USA) with constant stirring until the milk reached pH 4.25, which precipitated casein.

    Concentration Assay:

    Article Title: Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk
    Article Snippet: After quantification, only the milk with rhGH > 50 μg/mL was collected from the respective transgenic sows. .. Pretreatment of transgenic sow milk (sample preparation) The transgenic milk samples were diluted with a 2-fold volume of EDTA (Sigma, St. Louis, MO, USA) at a final concentration of 20 mM to extract rhGH within casein micelles. .. Defatted milk samples were acidified by slowly adding 50% acetic acid (Merck Millipore, Billerica, MA, USA) with constant stirring until the milk reached pH 4.25, which precipitated casein.

    other:

    Article Title: Tumour cell blebbing and extracellular vesicle shedding: key role of matrikines and ribosomal protein SA
    Article Snippet: Y27632, blebbistatin, U0126, PD150606, lactose, chondroitin sulphate, nifedipine and EDTA were from Sigma-Aldrich.

    Article Title: Zinc binding regulates amyloid-like aggregation of GAPR-1
    Article Snippet: Reagents Heparin was purchased from Santa Cruz Biotechnology (Heidelberg, Germany); mouse CRISP2 (cysteine-rich secretory protein) (UniProtKD ID: P16563), human CRISP3 (UniProtKD ID: P54108), and mouse CRISP4 (UniProtKD ID: Q9D259) from R & D Systems (Minneapolis, U.S.A.); trypsin from Thermo Fisher Scientific (Eindhoven, the Netherlands); Thioflavin T (ThT), ZnCl2 , ZnSO4 , and EDTA from Sigma–Aldrich (St. Louis, U.S.A.).

    Incubation:

    Article Title: Coagulation Factors IX and X Enhance Binding and Infection of Adenovirus Types 5 and 31 in Human Epithelial Cells ▿
    Article Snippet: Study of the effects of plasma components on the binding of Ad5 and Ad31 to target cells was performed essentially as described previously ( ). .. In some experiments, virions were preincubated with increasing concentrations of FIX or FX, with EDTA (10 mM), and/or with increasing concentrations of heparin (Sigma) before incubation with cells. .. In some experiments, cells were preincubated with increasing concentrations of heparinase I (Sigma) before incubation with virions.

    Protease Inhibitor:

    Article Title: Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System
    Article Snippet: .. The protease inhibitor cocktail for bacterial use and the protease inhibitors, including AEBSF, bestatin, pepstatin A, E-64 and EDTA, were purchased from Sigma-Aldrich (Milwaukee, WI, USA). .. An E. coli strain and plasmids, used for the construction of the E. coli secretion system, are listed in , and Top10 (an E. coli competent cell) and the T-vector were purchased from Invitrogen (Carlsbad, CA, USA) and Promega (Madison, WI, USA), respectively.

    Isolation:

    Article Title: Functional interaction of human Ssu72 with RNA polymerase II complexes
    Article Snippet: If complexes were to be isolated, see EC-EMSA protocol. .. If RNA was to be isolated, elongation complexes were stopped with stop solution containing 100 mM Tris pH 7.6, 0.2 mg/ml Torula Yeast RNA, 20 mM EDTA, and 1% Sarkosyl (Sigma, R6625). .. The RNA was then isolated by phenol extraction and precipitated by addition of 3 volumes of 95% ethanol containing 0.5 M ammonium acetate.

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  • 99
    Millipore tris edta
    ( a ) Concentration-dependent SERS spectra using AgNPs and <t>Tris-EDTA</t> in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.
    Tris Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris edta - by Bioz Stars, 2021-04
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    97
    Millipore edta
    Zn 2+ induces heparin-mediated GAPR-1 amyloid-like aggregation ( A ) Kinetics of <t>ThT</t> fluorescence enhancement of 15 μM GAPR-1 incubated with 37.5 μM heparin and 100 μM Zn 2+ . GAPR-1 incubated in the absence of heparin and/or Zn 2+ and in the presence of 1 mM <t>EDTA</t> are shown as controls. ( B ) ThT fluorescence enhancement of 15 μM GAPR-1 incubated with 37.5 μM heparin in the presence of increasing concentrations of Zn 2+ (0–1000 μM).
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    97
    Millipore edta or nta solution
    Characterization of binding interactions by ITC. (A,B) ITC titration of <t>EDTA</t> (1 mM) with Ca 2+ ions (10 mM) at 25°C in pH 7 (A) and pH 6.5 (B) . (C,D) <t>NTA</t> (1 mM) with Ca 2+ ions (10 mM) at 25°C in pH 7 (C) and pH 6.5 (D) . The reaction schemes for the formation of the Ca-EDTA complex (E) and Ca-NTA complex (F) .
    Edta Or Nta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta or nta solution/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta or nta solution - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    doi: 10.3390/s150510088

    Figure Lengend Snippet: ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Article Snippet: Chemicals Cr(III) and the other ionic substances of NaCl, KNO3 , Mg(NO3 )2 , Ca(NO3 )2 , Cr(NO3 )3 , Mn(NO3 )2 , FeC2 O4 , Fe(NO3 )3 , Co(NO3 )2 , Ni(NO3 )2 , Cu(NO3 )2 , Zn(NO3 )2 , NH4 NO3 , Cd(NO3 )2 , Hg(NO3 )2 , Pb(NO3 )2 , and K2 Cr2 O7 (or Na2 CrO4 ) along with silver nitrate, sodium citrate, Tris-EDTA (pH 8.0), and EDTA acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Concentration Assay

    ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    doi: 10.3390/s150510088

    Figure Lengend Snippet: ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Article Snippet: Chemicals Cr(III) and the other ionic substances of NaCl, KNO3 , Mg(NO3 )2 , Ca(NO3 )2 , Cr(NO3 )3 , Mn(NO3 )2 , FeC2 O4 , Fe(NO3 )3 , Co(NO3 )2 , Ni(NO3 )2 , Cu(NO3 )2 , Zn(NO3 )2 , NH4 NO3 , Cd(NO3 )2 , Hg(NO3 )2 , Pb(NO3 )2 , and K2 Cr2 O7 (or Na2 CrO4 ) along with silver nitrate, sodium citrate, Tris-EDTA (pH 8.0), and EDTA acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Raman Spectroscopy

    Zn 2+ induces heparin-mediated GAPR-1 amyloid-like aggregation ( A ) Kinetics of ThT fluorescence enhancement of 15 μM GAPR-1 incubated with 37.5 μM heparin and 100 μM Zn 2+ . GAPR-1 incubated in the absence of heparin and/or Zn 2+ and in the presence of 1 mM EDTA are shown as controls. ( B ) ThT fluorescence enhancement of 15 μM GAPR-1 incubated with 37.5 μM heparin in the presence of increasing concentrations of Zn 2+ (0–1000 μM).

    Journal: Bioscience Reports

    Article Title: Zinc binding regulates amyloid-like aggregation of GAPR-1

    doi: 10.1042/BSR20182345

    Figure Lengend Snippet: Zn 2+ induces heparin-mediated GAPR-1 amyloid-like aggregation ( A ) Kinetics of ThT fluorescence enhancement of 15 μM GAPR-1 incubated with 37.5 μM heparin and 100 μM Zn 2+ . GAPR-1 incubated in the absence of heparin and/or Zn 2+ and in the presence of 1 mM EDTA are shown as controls. ( B ) ThT fluorescence enhancement of 15 μM GAPR-1 incubated with 37.5 μM heparin in the presence of increasing concentrations of Zn 2+ (0–1000 μM).

    Article Snippet: Reagents Heparin was purchased from Santa Cruz Biotechnology (Heidelberg, Germany); mouse CRISP2 (cysteine-rich secretory protein) (UniProtKD ID: P16563), human CRISP3 (UniProtKD ID: P54108), and mouse CRISP4 (UniProtKD ID: Q9D259) from R & D Systems (Minneapolis, U.S.A.); trypsin from Thermo Fisher Scientific (Eindhoven, the Netherlands); Thioflavin T (ThT), ZnCl2 , ZnSO4 , and EDTA from Sigma–Aldrich (St. Louis, U.S.A.).

    Techniques: Fluorescence, Incubation

    EDP-induced blebbing depends on calcium influx and on RhoA/ROCK/MLC signalling pathway. a Measure of cytosolic calcium concentration using FURA-2 loaded HT-1080 cells in response to EDP stimulations. Calcium flux is reported as ratio 350 nm/380 nm fluorescence of FURA-2 in twenty HT-1080 cells. Data from one experiment, representative of five experiments are shown. Calcium-free media (0 Ca) were used to confirm the key role of the extracellular calcium. b Measure of cytosolic calcium concentration using FURA-2 loaded HT-1080 cells in presence of nifedipine after stimulation with EDPs. Calcium flux is reported as ratio 340 nm/380 nm fluorescence of FURA-2 in HT-1080 cells. The results shown are representative experiments ( n = 3). Nifedipine was used at 10 µM. c Blebbing inhibition in HT-1080 cells in the presence of EDTA, evaluated by counting 10 fields per well under a phase contrast optical microscope after EDP stimulation for 40 min. Data from one experiment, representative of three independent experiments, are shown. d Immunolocalisation by confocal microscopy of RhoA, ROCK2, ROCK1, P-LIMK1/2, cofilin, P-ERM, calpain1, actin and snapshot of mCherry-MLC transfected cells after treatment with EDPs versus untreated control HT-1080 cells (Ctl). Snapshot of 3D data was done using Imaris. Scale bar: 10 µm. e Schematic representation of the signalling pathways leading to EDP-induced blebbing and extracellular vesicle shedding . f Images from confocal microscopy analysis of cleaved caspase-3 and annexin-5 distributions after treatment with doxorubicin and EDPs. Cells were cultured on glass slides, fixed with paraformaldehyde and labelled with an anti-cleaved caspase-3 antibody (green) and annexin5-AF568 (red). Scale bar: 10 µm

    Journal: British Journal of Cancer

    Article Title: Tumour cell blebbing and extracellular vesicle shedding: key role of matrikines and ribosomal protein SA

    doi: 10.1038/s41416-019-0382-0

    Figure Lengend Snippet: EDP-induced blebbing depends on calcium influx and on RhoA/ROCK/MLC signalling pathway. a Measure of cytosolic calcium concentration using FURA-2 loaded HT-1080 cells in response to EDP stimulations. Calcium flux is reported as ratio 350 nm/380 nm fluorescence of FURA-2 in twenty HT-1080 cells. Data from one experiment, representative of five experiments are shown. Calcium-free media (0 Ca) were used to confirm the key role of the extracellular calcium. b Measure of cytosolic calcium concentration using FURA-2 loaded HT-1080 cells in presence of nifedipine after stimulation with EDPs. Calcium flux is reported as ratio 340 nm/380 nm fluorescence of FURA-2 in HT-1080 cells. The results shown are representative experiments ( n = 3). Nifedipine was used at 10 µM. c Blebbing inhibition in HT-1080 cells in the presence of EDTA, evaluated by counting 10 fields per well under a phase contrast optical microscope after EDP stimulation for 40 min. Data from one experiment, representative of three independent experiments, are shown. d Immunolocalisation by confocal microscopy of RhoA, ROCK2, ROCK1, P-LIMK1/2, cofilin, P-ERM, calpain1, actin and snapshot of mCherry-MLC transfected cells after treatment with EDPs versus untreated control HT-1080 cells (Ctl). Snapshot of 3D data was done using Imaris. Scale bar: 10 µm. e Schematic representation of the signalling pathways leading to EDP-induced blebbing and extracellular vesicle shedding . f Images from confocal microscopy analysis of cleaved caspase-3 and annexin-5 distributions after treatment with doxorubicin and EDPs. Cells were cultured on glass slides, fixed with paraformaldehyde and labelled with an anti-cleaved caspase-3 antibody (green) and annexin5-AF568 (red). Scale bar: 10 µm

    Article Snippet: Y27632, blebbistatin, U0126, PD150606, lactose, chondroitin sulphate, nifedipine and EDTA were from Sigma-Aldrich.

    Techniques: Concentration Assay, Fluorescence, Inhibition, Microscopy, Confocal Microscopy, Transfection, Cell Culture

    Characterization of binding interactions by ITC. (A,B) ITC titration of EDTA (1 mM) with Ca 2+ ions (10 mM) at 25°C in pH 7 (A) and pH 6.5 (B) . (C,D) NTA (1 mM) with Ca 2+ ions (10 mM) at 25°C in pH 7 (C) and pH 6.5 (D) . The reaction schemes for the formation of the Ca-EDTA complex (E) and Ca-NTA complex (F) .

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Microfluidic Encapsulation of Single Cells by Alginate Microgels Using a Trigger-Gellified Strategy

    doi: 10.3389/fbioe.2020.583065

    Figure Lengend Snippet: Characterization of binding interactions by ITC. (A,B) ITC titration of EDTA (1 mM) with Ca 2+ ions (10 mM) at 25°C in pH 7 (A) and pH 6.5 (B) . (C,D) NTA (1 mM) with Ca 2+ ions (10 mM) at 25°C in pH 7 (C) and pH 6.5 (D) . The reaction schemes for the formation of the Ca-EDTA complex (E) and Ca-NTA complex (F) .

    Article Snippet: To carry out the titration, the syringe was loaded with the CaCl2 solution, and the sample cuvette was filled with EDTA (or NTA) solution.

    Techniques: Binding Assay, Titration

    Gelation triggered by different calcium complexes. (A,B) Effect of water–oil flow rate ratio (Q aqu /Q oil ) and acetic acid concentration on the formation of alginate microgels when (A) Ca-NTA and (B) Ca-EDTA were used as the crosslinker, respectively. White and black areas indicated complete crosslink and uncrosslink, respectively. If the gelation process can be achieved on-chip, solid-like hydrogel particles can be obtained. Otherwise, only liquid can be collected since the gelation of the droplets cannot be realized within the short time window, for which we considered them as uncrosslinked microgels. (C) Gelation time in different acid concentrations where Ca 2+ ions were chelated in NTA or EDTA. (D) AutoCAD design of the microfluidic device for cell encapsulation. (E,F) Microscopic images of microchannels (E) for formation of alginate cell-laden microgels (F) . White arrows indicate cells. (G,H) Effect of Ca-NTA on microgel size and cell distribution. (G) Size distribution of alginate microgels. (H) Cell distribution in the microgels follows the Poisson distribution. Scale bar is 100 μm ( ***p

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Microfluidic Encapsulation of Single Cells by Alginate Microgels Using a Trigger-Gellified Strategy

    doi: 10.3389/fbioe.2020.583065

    Figure Lengend Snippet: Gelation triggered by different calcium complexes. (A,B) Effect of water–oil flow rate ratio (Q aqu /Q oil ) and acetic acid concentration on the formation of alginate microgels when (A) Ca-NTA and (B) Ca-EDTA were used as the crosslinker, respectively. White and black areas indicated complete crosslink and uncrosslink, respectively. If the gelation process can be achieved on-chip, solid-like hydrogel particles can be obtained. Otherwise, only liquid can be collected since the gelation of the droplets cannot be realized within the short time window, for which we considered them as uncrosslinked microgels. (C) Gelation time in different acid concentrations where Ca 2+ ions were chelated in NTA or EDTA. (D) AutoCAD design of the microfluidic device for cell encapsulation. (E,F) Microscopic images of microchannels (E) for formation of alginate cell-laden microgels (F) . White arrows indicate cells. (G,H) Effect of Ca-NTA on microgel size and cell distribution. (G) Size distribution of alginate microgels. (H) Cell distribution in the microgels follows the Poisson distribution. Scale bar is 100 μm ( ***p

    Article Snippet: To carry out the titration, the syringe was loaded with the CaCl2 solution, and the sample cuvette was filled with EDTA (or NTA) solution.

    Techniques: Concentration Assay, Chromatin Immunoprecipitation

    Evaluation of cell viability for microfluidic fabrication process. NIH3T3 fibroblasts were cultured in medium containing 1 wt% Na-alginate and 50 mM Ca-EDTA or Ca-NTA, incubated in a sealed syringe at 4°C and 37°C for 1 and 4 h, respectively. (A) The confocal microscopic images of live (green) and dead (red) cells stained by Calcein-AM and ethidium homodimer, respectively. The cells were incubated (B) 1 h and (C) 4 h. The numbers of live and dead cells were counted with the aid of ImageJ. The viability of NIH3T3 fibroblasts in microgels. To encapsulate cells, Ca-NTA and Ca-EDTA were used as the crosslinker. (D) Live (green) and dead (red) cells were stained by calcein-AM and ethidium homodimer, respectively. (E) The numbers of live and dead cells from microgels that were triggered by Ca-NTA or Ca-EDTA were counted with the aid of ImageJ. (F) Cell activity of encapsulated cells was assessed by CCK-8 assay after 24 h of culture. Scale bar is 200 μm ( ∗∗ p

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Microfluidic Encapsulation of Single Cells by Alginate Microgels Using a Trigger-Gellified Strategy

    doi: 10.3389/fbioe.2020.583065

    Figure Lengend Snippet: Evaluation of cell viability for microfluidic fabrication process. NIH3T3 fibroblasts were cultured in medium containing 1 wt% Na-alginate and 50 mM Ca-EDTA or Ca-NTA, incubated in a sealed syringe at 4°C and 37°C for 1 and 4 h, respectively. (A) The confocal microscopic images of live (green) and dead (red) cells stained by Calcein-AM and ethidium homodimer, respectively. The cells were incubated (B) 1 h and (C) 4 h. The numbers of live and dead cells were counted with the aid of ImageJ. The viability of NIH3T3 fibroblasts in microgels. To encapsulate cells, Ca-NTA and Ca-EDTA were used as the crosslinker. (D) Live (green) and dead (red) cells were stained by calcein-AM and ethidium homodimer, respectively. (E) The numbers of live and dead cells from microgels that were triggered by Ca-NTA or Ca-EDTA were counted with the aid of ImageJ. (F) Cell activity of encapsulated cells was assessed by CCK-8 assay after 24 h of culture. Scale bar is 200 μm ( ∗∗ p

    Article Snippet: To carry out the titration, the syringe was loaded with the CaCl2 solution, and the sample cuvette was filled with EDTA (or NTA) solution.

    Techniques: Cell Culture, Incubation, Staining, Activity Assay, CCK-8 Assay