Structured Review

Fisher Scientific edta
Edta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edta/product/Fisher Scientific
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
edta - by Bioz Stars, 2021-04
86/100 stars

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Related Articles

Isolation:

Article Title: Obesity-Dependent Increases in Oocyte mRNAs Are Associated With Increases in Proinflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice
Article Snippet: .. Protein extracts from one whole ovary from each female mouse (ND, n = 6; 45HFD, n = 6; 60HFD, n = 6) were isolated using ice-cold radioimmunoprecipitation assay buffer (RIPA) (65mM Tris-HCl [pH 7.4], 115mM NaCl, 1mM Na2 EDTA, 1mM EGTA, and 1% Triton X-100; Fisher Scientific) containing Halt Protease inhibitor cocktail (Sigma-Aldrich). .. Protein concentration was determined using bicnichoninic acid assay reagent according to the manufacturer's directions (Thermo Fisher).

Radio Immunoprecipitation:

Article Title: Obesity-Dependent Increases in Oocyte mRNAs Are Associated With Increases in Proinflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice
Article Snippet: .. Protein extracts from one whole ovary from each female mouse (ND, n = 6; 45HFD, n = 6; 60HFD, n = 6) were isolated using ice-cold radioimmunoprecipitation assay buffer (RIPA) (65mM Tris-HCl [pH 7.4], 115mM NaCl, 1mM Na2 EDTA, 1mM EGTA, and 1% Triton X-100; Fisher Scientific) containing Halt Protease inhibitor cocktail (Sigma-Aldrich). .. Protein concentration was determined using bicnichoninic acid assay reagent according to the manufacturer's directions (Thermo Fisher).

Protease Inhibitor:

Article Title: Obesity-Dependent Increases in Oocyte mRNAs Are Associated With Increases in Proinflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice
Article Snippet: .. Protein extracts from one whole ovary from each female mouse (ND, n = 6; 45HFD, n = 6; 60HFD, n = 6) were isolated using ice-cold radioimmunoprecipitation assay buffer (RIPA) (65mM Tris-HCl [pH 7.4], 115mM NaCl, 1mM Na2 EDTA, 1mM EGTA, and 1% Triton X-100; Fisher Scientific) containing Halt Protease inhibitor cocktail (Sigma-Aldrich). .. Protein concentration was determined using bicnichoninic acid assay reagent according to the manufacturer's directions (Thermo Fisher).

Polymerase Chain Reaction:

Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

Staining:

Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

Gel Extraction:

Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

High Performance Liquid Chromatography:

Article Title: Mucus Hydration in Subjects with Stable Chronic Bronchitis: A Comparison of Spontaneous and Induced Sputum
Article Snippet: A portion (100–500 mg) of the mucus gel plugs were diluted with guanidine reduction buffer (0.5%) for mucin analysis by size exclusion chromatography combined with differential refractometry. .. The remaining mucus gel plugs were diluted into a solution of dithiothreitol (0.1%) in EDTA (1mM) for analyses of: 1) sputum adenosine (ADO) and adenine nucleotides (ATP, ADP and AMP), ethenoderivatized and quantified by HPLC analysis ; and 2) total cell counts and differentials (Hema-Stain-3; Fisher Scientific, Hampton, NH). ..

other:

Article Title: Analysis of long non-coding RNAs produced by a specialized RNA Polymerase in Arabidopsis thaliana
Article Snippet: 10mM Tris-HCl pH8, Fisher Scientific (Tris: BP152-5, HCl: A144-500) 1mM EDTA, Fisher Scientific (EDTA: , NaOH: BP359-500)

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  • 86
    Fisher Scientific tris acetate ethylenediaminetetraacetic acid edta buffer
    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM <t>bis-tris</t> HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Tris Acetate Ethylenediaminetetraacetic Acid Edta Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris acetate ethylenediaminetetraacetic acid edta buffer/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris acetate ethylenediaminetetraacetic acid edta buffer - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific edta
    GO as a hydroxyl-radical scavenger. A: Addition of 100 ppm GO protects phenol red from oxidation by Fenton-generated · OH over 8 hrs. B: Addition of GO protects the spin-trap DMPO from oxidation by Fenton-generated · OH detected by EPR spectroscopy. The protective effect is concentration dependent over the range 0.1-90 ppm GO. C: Both phenol red monitoring and EPR were used to test if the GO-mediated protective effect is due to radical scavenging or Fenton suppression by Fe-binding. The protective effect of GO is still present when GO-Fe binding is suppressed by <t>EDTA,</t> confirming the importance of radical scavenging. D: GO also protects DMPO when · OH is generated sonochemically, in a non-Fenton assay, providing additional evidence for true radical scavenging. All experiments were done in <t>PBS</t> (pH 3.5).
    Edta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Journal: International Journal of Molecular Sciences

    Article Title: Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami

    doi: 10.3390/ijms19092513

    Figure Lengend Snippet: AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Article Snippet: The DNA scaffolds and corresponding staples were mixed in a 1:10 molar ratio, in a 1× tris-acetate-ethylenediaminetetraacetic acid (EDTA) buffer (Fisher Scientific, Hampton, NH, USA), with a pH of 8.3, and a [MgCl2 ] of 12.5 mM.

    Techniques: Incubation

    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Journal: International Journal of Molecular Sciences

    Article Title: Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami

    doi: 10.3390/ijms19092513

    Figure Lengend Snippet: AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Article Snippet: The DNA scaffolds and corresponding staples were mixed in a 1:10 molar ratio, in a 1× tris-acetate-ethylenediaminetetraacetic acid (EDTA) buffer (Fisher Scientific, Hampton, NH, USA), with a pH of 8.3, and a [MgCl2 ] of 12.5 mM.

    Techniques: Incubation

    GO as a hydroxyl-radical scavenger. A: Addition of 100 ppm GO protects phenol red from oxidation by Fenton-generated · OH over 8 hrs. B: Addition of GO protects the spin-trap DMPO from oxidation by Fenton-generated · OH detected by EPR spectroscopy. The protective effect is concentration dependent over the range 0.1-90 ppm GO. C: Both phenol red monitoring and EPR were used to test if the GO-mediated protective effect is due to radical scavenging or Fenton suppression by Fe-binding. The protective effect of GO is still present when GO-Fe binding is suppressed by EDTA, confirming the importance of radical scavenging. D: GO also protects DMPO when · OH is generated sonochemically, in a non-Fenton assay, providing additional evidence for true radical scavenging. All experiments were done in PBS (pH 3.5).

    Journal: Nanoscale

    Article Title: Antioxidant Chemistry of Graphene-Based Materials and its Role in Oxidation Protection Technology

    doi: 10.1039/c4nr03275f

    Figure Lengend Snippet: GO as a hydroxyl-radical scavenger. A: Addition of 100 ppm GO protects phenol red from oxidation by Fenton-generated · OH over 8 hrs. B: Addition of GO protects the spin-trap DMPO from oxidation by Fenton-generated · OH detected by EPR spectroscopy. The protective effect is concentration dependent over the range 0.1-90 ppm GO. C: Both phenol red monitoring and EPR were used to test if the GO-mediated protective effect is due to radical scavenging or Fenton suppression by Fe-binding. The protective effect of GO is still present when GO-Fe binding is suppressed by EDTA, confirming the importance of radical scavenging. D: GO also protects DMPO when · OH is generated sonochemically, in a non-Fenton assay, providing additional evidence for true radical scavenging. All experiments were done in PBS (pH 3.5).

    Article Snippet: Hydrogen peroxide (H2 O2 ), phosphate buffered saline (PBS) (pH 7.4), and EDTA were purchased from Fisher Scientific Inc. (Pittsburgh, PA).

    Techniques: Generated, Electron Paramagnetic Resonance, Spectroscopy, Concentration Assay, Binding Assay

    The effect of EDTA (left) and EGTA (right) on barnacle cement polymerization. Unpolymerized cement was incubated with EDTA, EGTA or deionized water for 2 min and then run on SDS-PAGE. Mean intensity (+s.e.m.) for the protein bands labeled in

    Journal: The Journal of Experimental Biology

    Article Title: Barnacle cement: a polymerization model based on evolutionary concepts

    doi: 10.1242/jeb.029884

    Figure Lengend Snippet: The effect of EDTA (left) and EGTA (right) on barnacle cement polymerization. Unpolymerized cement was incubated with EDTA, EGTA or deionized water for 2 min and then run on SDS-PAGE. Mean intensity (+s.e.m.) for the protein bands labeled in

    Article Snippet: EGTA (Sigma-Aldrich no. E4378) and EDTA (Fisher Chemical, no. BP118) were used.

    Techniques: Incubation, SDS Page, Labeling