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Becton Dickinson edta
Edta, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edta/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
edta - by Bioz Stars, 2021-04
86/100 stars

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Article Title: Determining the validity of hospital laboratory reference intervals for healthy young adults participating in early clinical trials of candidate vaccines
Article Snippet: Biochemistry samples were taken in lithium heparin and hematology samples in potassium EDTA (EDTA) using the Beckton Dickinson (BD) vacutainer collection system.

Article Title: EDTA-modified carbapenem inactivation method (eCIM) for detecting IMP Metallo-β-lactamase–producing Pseudomonas aeruginosa: an assessment of increasing EDTA concentrations
Article Snippet: Briefly, 2 mL of trypticase soy broth were inoculated with a 10-μL loopful of P. aeruginosa , vortexed, and a 10 μg meropenem disk (Becton Dickinson, Sparks, MD, LOT: 9065664 and 9186033) was placed into the mixture.

Article Title: Multiple Reaction Monitoring-based, Multiplexed, Absolute Quantitation of 45 Proteins in Human Plasma *
Article Snippet: Blood was collected by venous puncture using a 21-gauge BD Vacutainer Multiple Sample Needle (reference number 367213, BD Biosciences).

Article Title: Measurement of Hemostatic Factors in EDTA Plasma
Article Snippet: For the study, 30 mL of blood was collected from each volunteer and divided into aliquots for 2 EDTA tubes and 2 citrate Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ).

Concentration Assay:

Article Title: Comparison of phenotypic methods for the detection of carbapenem non-susceptible Enterobacteriaceae
Article Snippet: EDTA inhibition test This combination phenotypic detection method was performed using meropenem and meropenem-EDTA (292 μg). .. The stock solution of EDTA was prepared by dissolving anhydrous EDTA (Sigma-Aldrich, Milan, Italy) in distilled water at a concentration of 0.1 M. 10 μL of this solution were dispensed onto meropenem discs (BD BB™ Sensi-Disk™, Becton Dickinson Italia, Milan, Italy). .. The test was performed as a standard diffusion method, using one disk of meropenem and one containing meropenem and EDTA.

Incubation:

Article Title: EDTA-modified carbapenem inactivation method (eCIM) for detecting IMP Metallo-β-lactamase–producing Pseudomonas aeruginosa: an assessment of increasing EDTA concentrations
Article Snippet: .. All isolates were stored in skim milk (Becton Dickinson, Sparks, MD) at − 80 °C and subcultured to Trypticase soy agar with 5% sheep blood plates (Becton Dickinson, Sparks, MD) prior to incubation. .. Isolates were incubated, without selective disc pressure, at 37 °C for 18–20 h prior to second subculture before testing.

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  • 86
    Becton Dickinson edta tubes
    Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with <t>CPT</t> tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with <t>EDTA</t> tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.
    Edta Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta tubes/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta tubes - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson edta microtube
    Scatter plots and Bland-Altman plots comparing the reference CD4 counts versus Pima CD4. Scatter and Bland-Altman plots for capillary blood directly applied to CD4 cartridges, Pima-D (1A and 1B), capillary blood collected in <t>EDTA</t> <t>microtube,</t> Pima-M (2A and 2B), venous blood,Pima-V (3A and 3B), or venous blood tested with the Pima at the reference laboratory, Pima-Lab (4A and 4B) with reference CD4 assay being BD Multitest reagent and BD Trucount Tubes using a BD FACSCalibur.
    Edta Microtube, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta microtube/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta microtube - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson k3 edta vacutainer tubes
    Workstation setup. a Liquid handling workstation. b Tube rack workstation setup. The P1 rack contains centrifuged PB (Peripheral Blood) in BD <t>vacutainer</t> <t>EDTA</t> tubes. The S1 rack holds 50 mL conical tubes containing density gradient media, DPBS, DPBS + 2% FBS and an empty tube for waste. P3 contains the 15 mL conical tubes where the DPBS-diluted blood will be stratified onto 3 mL of the previously dispensed density gradient media. After centrifugation, the 15 mL conical tubes containing the stratified PB will be relocated to rack P3, and PBMCs will be transferred and diluted in the 15 mL conical tubes located in rack P4 containing 5 mL of DPBS + 2% FBS solution. DiTi disposable tips; LiHa liquid handling arms
    K3 Edta Vacutainer Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k3 edta vacutainer tubes/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k3 edta vacutainer tubes - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with CPT tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with EDTA tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.

    Journal: PLoS ONE

    Article Title: Evaluation of Peripheral Blood Mononuclear Cell Processing and Analysis for Survival Motor Neuron Protein

    doi: 10.1371/journal.pone.0050763

    Figure Lengend Snippet: Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with CPT tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with EDTA tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.

    Article Snippet: Whole blood samples at the Jasper Clinic were collected in BD CPT tubes (#362761, Studies 1–3), EDTA tubes (#22-040-67, Fisher, Pittsburgh, PA in Studies 3–5, and SMAF-001) or ACD tubes (#363083, BD, Franklin Lakes, NJ in Study 5).

    Techniques: Isolation, Cycling Probe Technology

    Scatter plots and Bland-Altman plots comparing the reference CD4 counts versus Pima CD4. Scatter and Bland-Altman plots for capillary blood directly applied to CD4 cartridges, Pima-D (1A and 1B), capillary blood collected in EDTA microtube, Pima-M (2A and 2B), venous blood,Pima-V (3A and 3B), or venous blood tested with the Pima at the reference laboratory, Pima-Lab (4A and 4B) with reference CD4 assay being BD Multitest reagent and BD Trucount Tubes using a BD FACSCalibur.

    Journal: PLoS ONE

    Article Title: Evaluation of Specimen Types for Pima CD4 Point-of-Care Testing: Advantages of Fingerstick Blood Collection into an EDTA Microtube

    doi: 10.1371/journal.pone.0202018

    Figure Lengend Snippet: Scatter plots and Bland-Altman plots comparing the reference CD4 counts versus Pima CD4. Scatter and Bland-Altman plots for capillary blood directly applied to CD4 cartridges, Pima-D (1A and 1B), capillary blood collected in EDTA microtube, Pima-M (2A and 2B), venous blood,Pima-V (3A and 3B), or venous blood tested with the Pima at the reference laboratory, Pima-Lab (4A and 4B) with reference CD4 assay being BD Multitest reagent and BD Trucount Tubes using a BD FACSCalibur.

    Article Snippet: They also showed that the drop-to-drip variation is reduced with cumulative volume, as would be see in using an EDTA microtube with fingerstick collection.

    Techniques:

    Workstation setup. a Liquid handling workstation. b Tube rack workstation setup. The P1 rack contains centrifuged PB (Peripheral Blood) in BD vacutainer EDTA tubes. The S1 rack holds 50 mL conical tubes containing density gradient media, DPBS, DPBS + 2% FBS and an empty tube for waste. P3 contains the 15 mL conical tubes where the DPBS-diluted blood will be stratified onto 3 mL of the previously dispensed density gradient media. After centrifugation, the 15 mL conical tubes containing the stratified PB will be relocated to rack P3, and PBMCs will be transferred and diluted in the 15 mL conical tubes located in rack P4 containing 5 mL of DPBS + 2% FBS solution. DiTi disposable tips; LiHa liquid handling arms

    Journal: Journal of Translational Medicine

    Article Title: Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation

    doi: 10.1186/s12967-019-2125-7

    Figure Lengend Snippet: Workstation setup. a Liquid handling workstation. b Tube rack workstation setup. The P1 rack contains centrifuged PB (Peripheral Blood) in BD vacutainer EDTA tubes. The S1 rack holds 50 mL conical tubes containing density gradient media, DPBS, DPBS + 2% FBS and an empty tube for waste. P3 contains the 15 mL conical tubes where the DPBS-diluted blood will be stratified onto 3 mL of the previously dispensed density gradient media. After centrifugation, the 15 mL conical tubes containing the stratified PB will be relocated to rack P3, and PBMCs will be transferred and diluted in the 15 mL conical tubes located in rack P4 containing 5 mL of DPBS + 2% FBS solution. DiTi disposable tips; LiHa liquid handling arms

    Article Snippet: Samples processing All samples were collected in 3 mL K3-EDTA vacutainer tubes (Becton–Dickinson, Ref. 368860), mixed immediately after the collection by inverting 10 times, and shipped to the lab in less than 30 min.

    Techniques: Centrifugation