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Sarstedt edta tubes
Edta Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edta tubes/product/Sarstedt
Average 86 stars, based on 1 article reviews
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edta tubes - by Bioz Stars, 2021-06
86/100 stars

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Mouse Assay:

Article Title: Novel evidence that crosstalk between the complement, coagulation and fibrinolysis proteolytic cascades is involved in mobilization of hematopoietic stem/progenitor cells (HSPCs)
Article Snippet: Plasma concentration of C5b-C9 (MAC complex) The concentration of C5b-C9 was measured by employing the commercially available, highly sensitive enzyme-linked immunosorbent assay (ELISA) kit K-ASSAY (Kamiya Biomedical Company, Seattle, WA, USA), according to the manufacturer's protocol. .. For analysis, PB from C3−/− mice was collected on day 6 of G-CSF-induced mobilization by retro-orbital plexus bleeding into cold microvette EDTA-coated tubes (Sarstedt Inc.). .. Activation of coagulation cascade PT and APTT were evaluated by employing coagulometry.

Article Title: PLASMA SPHINGOSINE 1-PHOSPHATE IS A MAJOR CHEMOATTRACTANT THAT DIRECTS EGRESS OF HEMATOPOIETIC STEM PROGENITOR CELLS FROM BONE MARROW AND ITS LEVEL IN PERIPHERAL BLOOD INCREASES DURING MOBILIZATION DUE TO ACTIVATION OF COMPLEMENT CASCADE/MEMBRANE ATTACK COMPLEX
Article Snippet: For G-CSF mobilization mice were injected subcutaneously (s.c.) with 250 μg/kg of human G-CSF (Amgen, Thousand Oaks, CA; http://www.amgen.com ) daily for 6 days. .. For zymosan mobilization, mice were injected intravenously (i.v.) with 0.5 mg/mouse and for AMD3100 mobilization with a dose of 5 mg/kg s.c. At 6 h after the last G-CSF administration or 1 h after zymosan or AMD3100 injection, mice were bled into microvette EDTA-coated tubes (Sarstedt Inc., Newton, NC; http://www.sarstedt.com) from the retro-orbital plexus to obtain a complete blood count (CBC) and 450 μl of PB was obtained from the vena cava with a 25-gauge needle and 1 ml syringe containing 50 μl of 100 mM ethylenediaminetetraacetic acid (EDTA). .. Some mice were treated with deoxypyridoxin (DOP, 30 mg/L) with glucose (10 g/L) in drinking water, as described elsewhere, for 14 hrs before BM harvest or AMD3100 administration.

Article Title: Effects of Fumaric Acids on Cuprizone Induced Central Nervous System De- and Remyelination in the Mouse
Article Snippet: The concentration of nitrite was determined by linear regression from a standard curve using known concentrations of sodium nitrite (Sigma-Aldrich Inc., St. Louis, MO) in culture medium. .. Induction of apoptosis of mouse PBMC To measure the effect of MMF and DMF on PBMC, blood from mice killed by cervical dislocation was collected by heart puncture into EDTA vacutainer tubes (Sarstedt, Newton, NC). ..

Flow Cytometry:

Article Title: Murine LRBA deficiency causes CTLA-4 deficiency in Tregs without progression to immune dysregulation
Article Snippet: .. Haematology and flow cytometry Red blood cells and platelet numbers were determined in blood collected from the tail-vein into EDTA (Sarstedt, North Rhine-Westphalia, Nümbrecht, Germany) tubes and analysed on a Sysmex XT-2000iV automated haematology analyser, Kobe, Hyōgo Prefecture, Japan. ..

Cytometry:

Article Title: Murine LRBA deficiency causes CTLA-4 deficiency in Tregs without progression to immune dysregulation
Article Snippet: .. Haematology and flow cytometry Red blood cells and platelet numbers were determined in blood collected from the tail-vein into EDTA (Sarstedt, North Rhine-Westphalia, Nümbrecht, Germany) tubes and analysed on a Sysmex XT-2000iV automated haematology analyser, Kobe, Hyōgo Prefecture, Japan. ..

Injection:

Article Title: PLASMA SPHINGOSINE 1-PHOSPHATE IS A MAJOR CHEMOATTRACTANT THAT DIRECTS EGRESS OF HEMATOPOIETIC STEM PROGENITOR CELLS FROM BONE MARROW AND ITS LEVEL IN PERIPHERAL BLOOD INCREASES DURING MOBILIZATION DUE TO ACTIVATION OF COMPLEMENT CASCADE/MEMBRANE ATTACK COMPLEX
Article Snippet: For G-CSF mobilization mice were injected subcutaneously (s.c.) with 250 μg/kg of human G-CSF (Amgen, Thousand Oaks, CA; http://www.amgen.com ) daily for 6 days. .. For zymosan mobilization, mice were injected intravenously (i.v.) with 0.5 mg/mouse and for AMD3100 mobilization with a dose of 5 mg/kg s.c. At 6 h after the last G-CSF administration or 1 h after zymosan or AMD3100 injection, mice were bled into microvette EDTA-coated tubes (Sarstedt Inc., Newton, NC; http://www.sarstedt.com) from the retro-orbital plexus to obtain a complete blood count (CBC) and 450 μl of PB was obtained from the vena cava with a 25-gauge needle and 1 ml syringe containing 50 μl of 100 mM ethylenediaminetetraacetic acid (EDTA). .. Some mice were treated with deoxypyridoxin (DOP, 30 mg/L) with glucose (10 g/L) in drinking water, as described elsewhere, for 14 hrs before BM harvest or AMD3100 administration.

Centrifugation:

Article Title: Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood
Article Snippet: Collection into Sarstedt S-Monovette serum-gel 7.5 ml tubes for serum was left at RT for 30 minutes for coagulation before centrifugation at 1,800 g for 15 minutes. .. Collection into Sarstedt S-Monovette EDTA 7.5 ml tubes for plasma was maintained at 4°C for 10–15 minutes before centrifugation at 3,000 g for 10 minutes. .. The resulting plasma and serum was transferred into 1.5 ml Lo-bind DNA tubes (Eppendorf) and snap frozen immediately.

Neutralization:

Article Title: Early decreased TLR2 expression on monocytes is associated with their reduced phagocytic activity and impaired maturation in a porcine polytrauma model
Article Snippet: .. TLR2 neutralization followed by phagocytosis assay Blood samples (EDTA tubes, Sarstedt) from ten male Pietrain pigs (Sus scrofa , 6 months old, 100 ± 5 kg) from a disease-free barrier breeding facility Bundes Hybrid Zucht Programm (BHZP) were drawn immediately after slaughtering for mechanistic studies. .. 50 μl were transferred into polystyrene FACS tubes (BD PharmingenTM ).

Phagocytosis Assay:

Article Title: Early decreased TLR2 expression on monocytes is associated with their reduced phagocytic activity and impaired maturation in a porcine polytrauma model
Article Snippet: .. TLR2 neutralization followed by phagocytosis assay Blood samples (EDTA tubes, Sarstedt) from ten male Pietrain pigs (Sus scrofa , 6 months old, 100 ± 5 kg) from a disease-free barrier breeding facility Bundes Hybrid Zucht Programm (BHZP) were drawn immediately after slaughtering for mechanistic studies. .. 50 μl were transferred into polystyrene FACS tubes (BD PharmingenTM ).

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  • 86
    Sarstedt dipotassium edta blood tube
    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in <t>EDTA</t> blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood <t>RNA</t> tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.
    Dipotassium Edta Blood Tube, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dipotassium edta blood tube/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dipotassium edta blood tube - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Sarstedt k3 edta coated tubes
    Comparison of the matrix effect of <t>K3</t> EDTA and lithium-heparin. Extraction of 60 μL plasma drawn with a K3 EDTA coated tube (A) and lithium-heparin coated tube (B); 5-CH 3 -H 4 folate m/z 460.20 → 313.20 (black), 460.20 → 180.15 (pink), and [ 13 C 5 ]-5-CH 3 -H 4 folate m/z 465.30 → 313.20 (blue), 460.30 → 180.15 (brown).
    K3 Edta Coated Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k3 edta coated tubes/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k3 edta coated tubes - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Sarstedt edta coated tubes
    Five-week-old ApoE −/− mice were exposed to two different concentrations of arsenic (200 or 1000 ppb) for 8 weeks or maintained on tap water. For <t>cytokine</t> measurements, blood was collected by terminal cardiac puncture into <t>EDTA-coated</t> tube
    Edta Coated Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta coated tubes/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta coated tubes - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Sarstedt s monovette edta gel tubes
    Five-week-old ApoE −/− mice were exposed to two different concentrations of arsenic (200 or 1000 ppb) for 8 weeks or maintained on tap water. For <t>cytokine</t> measurements, blood was collected by terminal cardiac puncture into <t>EDTA-coated</t> tube
    S Monovette Edta Gel Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s monovette edta gel tubes/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s monovette edta gel tubes - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Journal: PLoS ONE

    Article Title: Towards Clinical Applications of Blood-Borne miRNA Signatures: The Influence of the Anticoagulant EDTA on miRNA Abundance

    doi: 10.1371/journal.pone.0143321

    Figure Lengend Snippet: Microarray present call analysis. A) Average number (Y-axis) and standard deviation of miRNA detected in blood samples of six healthy individuals under four different blood sampling conditions (X-axis). Blood was collected in EDTA blood collection tubes and subsequently transferred into PAXgene TM tubes at three different time points, i.e. directly (0 min), 10 min, and 2 h after phlebotomy. As control blood was also directly collected in PAXgene TM blood RNA tubes. B) Venn diagram of miRNAs that were present in all six individuals under different blood sampling conditions. There were 201 miRNAs detected in all individuals under all conditions. No miRNA was detected both in blood that was directly collected in PAXgene TM blood RNA tubes and in blood that was transferred into PAXgene TM tubes 2 h after phlebotomy without also being detected in blood directly transferred or transferred 10 min after phlebotomy. C) Balloon plot of miRNAs that show a difference in frequency under the different blood sampling conditions. The X-axis shows the minimal number of individuals that are positive for a miRNA under one condition. The Y-axis shows the maximum number of individuals that are positive for a miRNA under one condition. The top left balloon denotes the 806 miRNAs that were not detected in any of the 24 samples (minimum and maximum of 0). The lower right balloon denotes the 201 miRNAs that were detected in all samples (minimum and maximum of 6). The orange shaded area presents 12 miRNAs that show a difference in frequency of at least three under one of the four conditions as compared to the other conditions. The largest difference was found for miR-769-3p indicated in the lower left corner that was positive in 6 samples under the 2h EDTA condition and not in any sample of the PAXgene condition.

    Article Snippet: Sample collection Blood from 6 healthy individuals was collected in one PAXgeneTM blood RNA tube (Becton Dickinson, 2.5 ml blood) and one dipotassium EDTA blood tube (EDTA-KE Monovette, Sarstedt, 9 ml blood) per individual.

    Techniques: Microarray, Standard Deviation, Sampling

    Comparison of the matrix effect of K3 EDTA and lithium-heparin. Extraction of 60 μL plasma drawn with a K3 EDTA coated tube (A) and lithium-heparin coated tube (B); 5-CH 3 -H 4 folate m/z 460.20 → 313.20 (black), 460.20 → 180.15 (pink), and [ 13 C 5 ]-5-CH 3 -H 4 folate m/z 465.30 → 313.20 (blue), 460.30 → 180.15 (brown).

    Journal: PLoS ONE

    Article Title: Quantitation of 5-methyltetraydrofolic acid in plasma for determination of folate status and clinical studies by stable isotope dilution assays

    doi: 10.1371/journal.pone.0212255

    Figure Lengend Snippet: Comparison of the matrix effect of K3 EDTA and lithium-heparin. Extraction of 60 μL plasma drawn with a K3 EDTA coated tube (A) and lithium-heparin coated tube (B); 5-CH 3 -H 4 folate m/z 460.20 → 313.20 (black), 460.20 → 180.15 (pink), and [ 13 C 5 ]-5-CH 3 -H 4 folate m/z 465.30 → 313.20 (blue), 460.30 → 180.15 (brown).

    Article Snippet: Venous blood samples (9 mL, 60 μL were used for extraction) were drawn in K3 EDTA-coated tubes (Sarstedt, Nürnbrecht, Germany) at defined time intervals: before the administration of the test food, every 15 min for 2 h after the administration of the test food, every hour up to 10 h after the initial 2 h. Blood was immediately centrifuged (10 min, 2.500 rpm, 4°C), and plasma was stored with a saturated amount of ascorbic acid at −20°C in the dark until extraction.

    Techniques:

    Five-week-old ApoE −/− mice were exposed to two different concentrations of arsenic (200 or 1000 ppb) for 8 weeks or maintained on tap water. For cytokine measurements, blood was collected by terminal cardiac puncture into EDTA-coated tube

    Journal: Toxicological Sciences

    Article Title: Exposure to Moderate Arsenic Concentrations Increases Atherosclerosis in ApoE−/− Mouse Model

    doi: 10.1093/toxsci/kfr097

    Figure Lengend Snippet: Five-week-old ApoE −/− mice were exposed to two different concentrations of arsenic (200 or 1000 ppb) for 8 weeks or maintained on tap water. For cytokine measurements, blood was collected by terminal cardiac puncture into EDTA-coated tube

    Article Snippet: For cytokine measurements, blood was collected in EDTA-coated tubes (Sarstedt, Germany) and the cytokine levels (macrophage inflammatory protein-1 [MIP1α] and interferon-γ [IFNγ]) were measured in the plasma using an immunoassay kit (multiplex bead-based) on a Bio-Plex 200 (Bio-Rad Laboratories, ON, Canada).

    Techniques: Mouse Assay