Structured Review

GE Healthcare edta plasma supernatant
Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol <t>(PEG)-precipitated</t> activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to <t>EDTA</t> plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.
Edta Plasma Supernatant, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edta plasma supernatant/product/GE Healthcare
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
edta plasma supernatant - by Bioz Stars, 2020-05
92/100 stars

Related Products / Commonly Used Together

superose
peg
gpc
gel permeation chromatography gpc

Images

1) Product Images from "The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus"

Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2018.00581

Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.
Figure Legend Snippet: Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.

Techniques Used: GPC Assay, Gel Permeation Chromatography, Activation Assay, Concentration Assay, Western Blot, SDS Page

2) Product Images from "The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus"

Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2018.00581

Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.
Figure Legend Snippet: Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.

Techniques Used: GPC Assay, Gel Permeation Chromatography, Activation Assay, Concentration Assay, Western Blot, SDS Page

Related Articles

Gel Permeation Chromatography:

Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus
Article Snippet: .. Samples of serum, supernatant of PEG precipitated activated serum and EDTA-plasma supernatant after precipitation with either 11 or 16% PEG (w/v), were subjected to GPC on a Superose 6 10/300 GL column (GE Healthcare). ..

Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus
Article Snippet: .. Gel Permeation Chromatography (GPC) Samples of serum, supernatant of PEG precipitated activated serum and EDTA-plasma supernatant after precipitation with either 11 or 16% PEG (w/v), were subjected to GPC on a Superose 6 10/300 GL column (GE Healthcare). ..

GPC Assay:

Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus
Article Snippet: .. Gel Permeation Chromatography (GPC) Samples of serum, supernatant of PEG precipitated activated serum and EDTA-plasma supernatant after precipitation with either 11 or 16% PEG (w/v), were subjected to GPC on a Superose 6 10/300 GL column (GE Healthcare). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    GE Healthcare edta plasma supernatant
    Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol <t>(PEG)-precipitated</t> activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to <t>EDTA</t> plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.
    Edta Plasma Supernatant, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta plasma supernatant/product/GE Healthcare
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    edta plasma supernatant - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.

    Journal: Frontiers in Immunology

    Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus

    doi: 10.3389/fimmu.2018.00581

    Figure Lengend Snippet: Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.

    Article Snippet: Samples of serum, supernatant of PEG precipitated activated serum and EDTA-plasma supernatant after precipitation with either 11 or 16% PEG (w/v), were subjected to GPC on a Superose 6 10/300 GL column (GE Healthcare).

    Techniques: GPC Assay, Gel Permeation Chromatography, Activation Assay, Concentration Assay, Western Blot, SDS Page

    Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.

    Journal: Frontiers in Immunology

    Article Title: The C3dg Fragment of Complement Is Superior to Conventional C3 as a Diagnostic Biomarker in Systemic Lupus Erythematosus

    doi: 10.3389/fimmu.2018.00581

    Figure Lengend Snippet: Separation of C3dg from the larger C3 fragments. Measurements of C3 molecules encompassing the determinants of the segment C3dg, i.e., C3 and all degradation molecules containing this part of C3. (A) The figure illustrates C3dg measurement on gel permeation chromatography (GPC) fractions of serum before activation (red line) and supernatant of polyethylene glycol (PEG)-precipitated activated serum (blue line). (B) Test for the optimal concentration of PEG for precipitation. Increasing concentrations of PEG were added to EDTA plasma and C3dg was estimated in the supernatants. (C) GPC of supernatant after precipitation of EDTA plasma with 11% (red) and 16% (blue) PEG. C3dg in the fractions was measured. The 11% supernatant shows two major peaks, the first corresponding to C3 and larger C3 components, and the second corresponding to free C3dg. After precipitation with 16% PEG the first was significantly reduced. Panel (D) shows the results of western blotting of supernatants of EDTA-plasma precipitated with 16% (lane 1) or 11% (lane 2) PEG. The samples (corresponding to 0.1 µl plasma) were run non-reduced on the SDS-PAGE. The blot was developed with anti-C3d antibody. It can be seen that the 11% PEG supernatant still contains appreciable amounts of larger C3dg-encompassing molecules. This was repeated three times with similar results.

    Article Snippet: Gel Permeation Chromatography (GPC) Samples of serum, supernatant of PEG precipitated activated serum and EDTA-plasma supernatant after precipitation with either 11 or 16% PEG (w/v), were subjected to GPC on a Superose 6 10/300 GL column (GE Healthcare).

    Techniques: GPC Assay, Gel Permeation Chromatography, Activation Assay, Concentration Assay, Western Blot, SDS Page