edta free protease inhibitor cocktail (Millipore)
99
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Name:
cOmplete EDTA free Protease Inhibitor Cocktail
Description:
cOmplete EDTA free Protease Inhibitor Tablets inhibit a broad spectrum of serine and cysteine proteases In contrast to other cOmplete tablets they do not contain EDTA no other chelating agents e g EGTA as well leaving the stability and the function of metal dependant proteins unaffected The affinity purification of Poly His tagged fusion proteins using IMAC Immobilized Metal Affinity Chromatography is also facilitated no dialysis necessary Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases and are well suited for the protection of proteins isolated from animal tissues plants yeast and bacteria cOmplete EDTA free tablets contains both irreversible and reversible protease inhibitors Metallo and aspartic proteases are not inhibited cOmplete EDTA free Tablets are identical to cOmplete Tablets the only difference being that no EDTA or other chelating e g EGTA agents are included
Catalog Number:
04693132001
Price:
None
Applications:
cOmplete(TM), EDTA-free, efficiently inhibits serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts. Metallo- and aspartic proteases are not inhibited.cOmplete, EDTA-free Tablets, are used for the inhibition of proteolytic activity in large volumes (up to 50ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, EDTA-free is preferentially used in the isolation process of Poly-His-tagged fusion proteins.If it is necessary to inhibit proteolytic activity in a smaller volume (up to 10ml), we recommend to use cOmplete, Mini, EDTA-free.One cOmplete, EDTA-free tablet was added per 50 ml incubation solution. Proteolytic activity was determined with the Roche Universal Protease Substrate (casein, resorufin-labeled). When extractions or single-step isolations are necessary in the acid pH range, simply include pepstatin along with cOmplete, EDTA-free tablets to ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25C.
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Structured Review
Millipore
edta free protease inhibitor cocktail
cOmplete EDTA free Protease Inhibitor Tablets inhibit a broad spectrum of serine and cysteine proteases In contrast to other cOmplete tablets they do not contain EDTA no other chelating agents e g EGTA as well leaving the stability and the function of metal dependant proteins unaffected The affinity purification of Poly His tagged fusion proteins using IMAC Immobilized Metal Affinity Chromatography is also facilitated no dialysis necessary Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases and are well suited for the protection of proteins isolated from animal tissues plants yeast and bacteria cOmplete EDTA free tablets contains both irreversible and reversible protease inhibitors Metallo and aspartic proteases are not inhibited cOmplete EDTA free Tablets are identical to cOmplete Tablets the only difference being that no EDTA or other chelating e g EGTA agents are included
https://www.bioz.com/result/edta free protease inhibitor cocktail/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cOmplete EDTA free Protease Inhibitor Tablets inhibit a broad spectrum of serine and cysteine proteases In contrast to other cOmplete tablets they do not contain EDTA no other chelating agents e g EGTA as well leaving the stability and the function of metal dependant proteins unaffected The affinity purification of Poly His tagged fusion proteins using IMAC Immobilized Metal Affinity Chromatography is also facilitated no dialysis necessary Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases and are well suited for the protection of proteins isolated from animal tissues plants yeast and bacteria cOmplete EDTA free tablets contains both irreversible and reversible protease inhibitors Metallo and aspartic proteases are not inhibited cOmplete EDTA free Tablets are identical to cOmplete Tablets the only difference being that no EDTA or other chelating e g EGTA agents are included
https://www.bioz.com/result/edta free protease inhibitor cocktail/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
edta free protease inhibitor cocktail - by Bioz Stars,
2021-03
99/100 stars
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