eda adp  (Jena Bioscience)


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  • 85
    Name:
    EDA ADP
    Description:

    Catalog Number:
    NU-802L
    Price:
    352.0
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 30 µl
    Buy from Supplier


    Structured Review

    Jena Bioscience eda adp

    https://www.bioz.com/result/eda adp/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eda adp - by Bioz Stars, 2021-06
    85/100 stars

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    other:

    Article Title: Isolation, Characterization and Lipid-Binding Properties of the Recalcitrant FtsA Division Protein from Escherichia coli
    Article Snippet: The retention of E. coli FtsA in EDA-ADP (Jena Biosciences) or ATP-agarose columns (ATP affinity test kit, Jena Biosciences) was tested following manufacturer’s specifications.

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  • 91
    Jena Bioscience n m eda adp atto 495
    P472L mutation does not disrupt the binding sites of p97 inhibitors. A, ITC was used to measure the binding of CB-5083 to p97 and the P472L mutant. Top panels are raw data, and lower panels show isotherms with fitted curves and representative K D values. B, effect of increasing NMS-873 concentrations on TR-FRET generated by p97 and the P472L mutant in the presence of BODIPY-FL–ATP, and a terbium-labeled anti-His tag antibody was measured ( n = 4, S.D.). Apparent K D values extrapolated from NMS-873–dependent increases in TR-FRET are shown. C, fluorescence polarization experiments were performed ( n = 2, S.D.) to measure the enhanced binding of <t>EDA–ADP–ATTO-495</t> to p97 and the P472L mutant with increasing NMS-873 concentrations. Apparent K D values with S.E. are shown.
    N M Eda Adp Atto 495, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n m eda adp atto 495/product/Jena Bioscience
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n m eda adp atto 495 - by Bioz Stars, 2021-06
    91/100 stars
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    85
    Jena Bioscience eda adp atto 590 probe
    P472L mutation does not disrupt the binding sites of p97 inhibitors. A, ITC was used to measure the binding of CB-5083 to p97 and the P472L mutant. Top panels are raw data, and lower panels show isotherms with fitted curves and representative K D values. B, effect of increasing NMS-873 concentrations on TR-FRET generated by p97 and the P472L mutant in the presence of BODIPY-FL–ATP, and a terbium-labeled anti-His tag antibody was measured ( n = 4, S.D.). Apparent K D values extrapolated from NMS-873–dependent increases in TR-FRET are shown. C, fluorescence polarization experiments were performed ( n = 2, S.D.) to measure the enhanced binding of <t>EDA–ADP–ATTO-495</t> to p97 and the P472L mutant with increasing NMS-873 concentrations. Apparent K D values with S.E. are shown.
    Eda Adp Atto 590 Probe, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eda adp atto 590 probe/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eda adp atto 590 probe - by Bioz Stars, 2021-06
    85/100 stars
      Buy from Supplier

    85
    Jena Bioscience eda adp
    P472L mutation does not disrupt the binding sites of p97 inhibitors. A, ITC was used to measure the binding of CB-5083 to p97 and the P472L mutant. Top panels are raw data, and lower panels show isotherms with fitted curves and representative K D values. B, effect of increasing NMS-873 concentrations on TR-FRET generated by p97 and the P472L mutant in the presence of BODIPY-FL–ATP, and a terbium-labeled anti-His tag antibody was measured ( n = 4, S.D.). Apparent K D values extrapolated from NMS-873–dependent increases in TR-FRET are shown. C, fluorescence polarization experiments were performed ( n = 2, S.D.) to measure the enhanced binding of <t>EDA–ADP–ATTO-495</t> to p97 and the P472L mutant with increasing NMS-873 concentrations. Apparent K D values with S.E. are shown.
    Eda Adp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eda adp/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eda adp - by Bioz Stars, 2021-06
    85/100 stars
      Buy from Supplier


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    P472L mutation does not disrupt the binding sites of p97 inhibitors. A, ITC was used to measure the binding of CB-5083 to p97 and the P472L mutant. Top panels are raw data, and lower panels show isotherms with fitted curves and representative K D values. B, effect of increasing NMS-873 concentrations on TR-FRET generated by p97 and the P472L mutant in the presence of BODIPY-FL–ATP, and a terbium-labeled anti-His tag antibody was measured ( n = 4, S.D.). Apparent K D values extrapolated from NMS-873–dependent increases in TR-FRET are shown. C, fluorescence polarization experiments were performed ( n = 2, S.D.) to measure the enhanced binding of EDA–ADP–ATTO-495 to p97 and the P472L mutant with increasing NMS-873 concentrations. Apparent K D values with S.E. are shown.

    Journal: The Journal of Biological Chemistry

    Article Title: Adapted ATPase domain communication overcomes the cytotoxicity of p97 inhibitors

    doi: 10.1074/jbc.RA118.004301

    Figure Lengend Snippet: P472L mutation does not disrupt the binding sites of p97 inhibitors. A, ITC was used to measure the binding of CB-5083 to p97 and the P472L mutant. Top panels are raw data, and lower panels show isotherms with fitted curves and representative K D values. B, effect of increasing NMS-873 concentrations on TR-FRET generated by p97 and the P472L mutant in the presence of BODIPY-FL–ATP, and a terbium-labeled anti-His tag antibody was measured ( n = 4, S.D.). Apparent K D values extrapolated from NMS-873–dependent increases in TR-FRET are shown. C, fluorescence polarization experiments were performed ( n = 2, S.D.) to measure the enhanced binding of EDA–ADP–ATTO-495 to p97 and the P472L mutant with increasing NMS-873 concentrations. Apparent K D values with S.E. are shown.

    Article Snippet: The 2-fold serial dilutions of p97 proteins (maximum 40 μ m , purified from bacteria) were mixed with 20 n m BODIPY-FL–ATP (ThermoFisher Scientific) or 10 n m EDA–ADP–ATTO-495 (Jena Bioscience) in black low volume 384-well plates.

    Techniques: Mutagenesis, Binding Assay, Generated, Labeling, Fluorescence