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    Thermo Fisher ed 1 positive macrophages
    Macrophage activation induces axon regeneration in the rat optic nerve. Sections through the retina ( a , c , e ) or the optic nerve ( b , d , f ) were stained with antibodies to detect GAP-43 ( a – f , green fluorescence) or the macrophage marker <t>ED-1</t> ( a , c , e , red fluorescence) 2 weeks after optic nerve surgery. a , GAP-43 is not detected in the GCL ( open arrowheads ) of animals that had received control PBS injections after optic nerve damage; ED-1 + macrophages are absent. b , Few GAP-43-positive axons extend past the injury site ( asterisk ) in the optic nerve. c , Zymosan injected into the vitreous the same day as nerve injury stimulates ED-1 + macrophages to infiltrate the eye and distribute near the GCL ( arrows ). GAP-43 expression is intense in RGC somata ( arrowheads ), and many GAP-43-positive axons extend into the distal optic nerve ( d ). e , Zymosan injections made 3 d after nerve injury result in high levels of GAP-43 in RGCs ( arrowheads ) and greater numbers of GAP-43-positive axons extending distal to the injury site ( f ). Scale bars: a , c , e , 250 μm; b , d , f , 200 μm.
    Ed 1 Positive Macrophages, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ed 1 positive macrophages/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ed 1 positive macrophages - by Bioz Stars, 2021-06
    95/100 stars

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    1) Product Images from "Macrophage-Derived Factors Stimulate Optic Nerve Regeneration"

    Article Title: Macrophage-Derived Factors Stimulate Optic Nerve Regeneration

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.23-06-02284.2003

    Macrophage activation induces axon regeneration in the rat optic nerve. Sections through the retina ( a , c , e ) or the optic nerve ( b , d , f ) were stained with antibodies to detect GAP-43 ( a – f , green fluorescence) or the macrophage marker ED-1 ( a , c , e , red fluorescence) 2 weeks after optic nerve surgery. a , GAP-43 is not detected in the GCL ( open arrowheads ) of animals that had received control PBS injections after optic nerve damage; ED-1 + macrophages are absent. b , Few GAP-43-positive axons extend past the injury site ( asterisk ) in the optic nerve. c , Zymosan injected into the vitreous the same day as nerve injury stimulates ED-1 + macrophages to infiltrate the eye and distribute near the GCL ( arrows ). GAP-43 expression is intense in RGC somata ( arrowheads ), and many GAP-43-positive axons extend into the distal optic nerve ( d ). e , Zymosan injections made 3 d after nerve injury result in high levels of GAP-43 in RGCs ( arrowheads ) and greater numbers of GAP-43-positive axons extending distal to the injury site ( f ). Scale bars: a , c , e , 250 μm; b , d , f , 200 μm.
    Figure Legend Snippet: Macrophage activation induces axon regeneration in the rat optic nerve. Sections through the retina ( a , c , e ) or the optic nerve ( b , d , f ) were stained with antibodies to detect GAP-43 ( a – f , green fluorescence) or the macrophage marker ED-1 ( a , c , e , red fluorescence) 2 weeks after optic nerve surgery. a , GAP-43 is not detected in the GCL ( open arrowheads ) of animals that had received control PBS injections after optic nerve damage; ED-1 + macrophages are absent. b , Few GAP-43-positive axons extend past the injury site ( asterisk ) in the optic nerve. c , Zymosan injected into the vitreous the same day as nerve injury stimulates ED-1 + macrophages to infiltrate the eye and distribute near the GCL ( arrows ). GAP-43 expression is intense in RGC somata ( arrowheads ), and many GAP-43-positive axons extend into the distal optic nerve ( d ). e , Zymosan injections made 3 d after nerve injury result in high levels of GAP-43 in RGCs ( arrowheads ) and greater numbers of GAP-43-positive axons extending distal to the injury site ( f ). Scale bars: a , c , e , 250 μm; b , d , f , 200 μm.

    Techniques Used: Activation Assay, Staining, Fluorescence, Marker, Injection, Expressing

    Effect of Zymosan dosage on RGC survival. a , Zymosan injections, either on the day of nerve crush ( D0 ) or 3 d later ( D3 ), increased the number of TUJ1 + cells in the retina 3 weeks after a peripheral nerve graft. Zymosan resulted in more surviving TUJ1 + cells when injected at a low concentration (1.25 μg/μl) than at a high concentration (12.5 μg/μl). b , The higher dosage of Zymosan diminished retinal size. c , Normal retina (flat mounted). No macrophages appear in the vitreous or around the GCL, although some ED-1 + microglia are seen ( arrowhead ). d , Axotomy followed by a PN graft increases the number of ED-1 + monocytes ( arrow, arrowheads ) in the retina only slightly. e , Zymosan injections (1.25 μg/μl) result in accumulation of ED-1 + macrophages ( arrows ) in the vitreous and around the GCL. * p
    Figure Legend Snippet: Effect of Zymosan dosage on RGC survival. a , Zymosan injections, either on the day of nerve crush ( D0 ) or 3 d later ( D3 ), increased the number of TUJ1 + cells in the retina 3 weeks after a peripheral nerve graft. Zymosan resulted in more surviving TUJ1 + cells when injected at a low concentration (1.25 μg/μl) than at a high concentration (12.5 μg/μl). b , The higher dosage of Zymosan diminished retinal size. c , Normal retina (flat mounted). No macrophages appear in the vitreous or around the GCL, although some ED-1 + microglia are seen ( arrowhead ). d , Axotomy followed by a PN graft increases the number of ED-1 + monocytes ( arrow, arrowheads ) in the retina only slightly. e , Zymosan injections (1.25 μg/μl) result in accumulation of ED-1 + macrophages ( arrows ) in the vitreous and around the GCL. * p

    Techniques Used: Injection, Concentration Assay

    Related Articles

    Next-Generation Sequencing:

    Article Title: Controlled release of neurotrophin-3 and platelet derived growth factor from fibrin scaffolds containing neural progenitor cells enhances survival and differentiation into neurons in a subacute model of SCI †
    Article Snippet: Primary antibodies against glial fibrillary acidic protein (GFAP, rabbit polyclonal, recognizing astrocytes, 1:4, ImmunoStar, Hudson, WI), neuronal class III β-tubulin (Tuj1, mouse monoclonal, recognizing neurons, 1:200, Covance Research Products, Berkeley, CA), ED-1 (mouse monoclonal, recognizing macrophages/microglia, 1:100, Serotec, Oxford, UK), anti-oligodendrocyte marker O4 (O4, mouse monoclonal, recognizes oligodendrocytes, 1:200, Millipore ), anti-nestin (Nestin, mouse monoclonal, recognizes NPCs, 1:200, Millipore), anti-neuronal nuclei (NeuN, mouse monoclonal, recognizes mature neurons, 1:500, Millipore) and anti-SSEA-1 (SSEA-1, mouse monoclonal, recognizes mouse ESCs, 1:50, Millipore) were used to evaluate each section. .. Finally, appropriate secondary antibodies (Alexa Fluor 555, and 647 conjugated, 1:300 Invitrogen, Carlsbad, CA) diluted with 2% NGS were used and each section was stained with Hoechst nuclear stain (1:1000 Molecular Probes). ..

    Staining:

    Article Title: Controlled release of neurotrophin-3 and platelet derived growth factor from fibrin scaffolds containing neural progenitor cells enhances survival and differentiation into neurons in a subacute model of SCI †
    Article Snippet: Primary antibodies against glial fibrillary acidic protein (GFAP, rabbit polyclonal, recognizing astrocytes, 1:4, ImmunoStar, Hudson, WI), neuronal class III β-tubulin (Tuj1, mouse monoclonal, recognizing neurons, 1:200, Covance Research Products, Berkeley, CA), ED-1 (mouse monoclonal, recognizing macrophages/microglia, 1:100, Serotec, Oxford, UK), anti-oligodendrocyte marker O4 (O4, mouse monoclonal, recognizes oligodendrocytes, 1:200, Millipore ), anti-nestin (Nestin, mouse monoclonal, recognizes NPCs, 1:200, Millipore), anti-neuronal nuclei (NeuN, mouse monoclonal, recognizes mature neurons, 1:500, Millipore) and anti-SSEA-1 (SSEA-1, mouse monoclonal, recognizes mouse ESCs, 1:50, Millipore) were used to evaluate each section. .. Finally, appropriate secondary antibodies (Alexa Fluor 555, and 647 conjugated, 1:300 Invitrogen, Carlsbad, CA) diluted with 2% NGS were used and each section was stained with Hoechst nuclear stain (1:1000 Molecular Probes). ..

    Article Title: Plasmid Releasing Multiple Channel Bridges for Transgene Expression After Spinal Cord Injury
    Article Snippet: A Hoechst stain was coincidently performed with the stain for EGFP to validate the position of the cells. .. Every 6th collected section was double stained for a specific cell stain with primary monoclonal mouse IgG1 antibodies [(i) anti-S100 (β-subunit) for Schwann cells (Sigma); (ii) anti-oligodendrocytes (RIP) (Millipore, Billerica, MA); (iii) anti-rat monocytes/macrophages (CD68) (ED-1) (Millipore, Billerica, MA); (iv) anti-glial fibrillary acidic protein (GFAP) for reactive astrocytes (Sigma, St. Louis, MO); (v) anti-rat prolyl 4-hydroxylase (rPH) for fibroblasts (Acris Antibodies, Herford, Germany); and (vi) anti-rat RECA-1 for endothelial cells (AbD Serotec, Raleigh, NC)] and Alexa Fluor 546 goat anti-mouse (red) (Invitrogen, Carlsbad, CA) as a secondary antibody. .. Pictures were taken of three random fields in the bridge and three random fields in the adjacent tissue (×20) with red and green fluorescent filter sets using Metaview software.

    Activity Assay:

    Article Title: Pharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injury
    Article Snippet: Immunohistochemistry Caspase-3, nitrotyrosine, macrophages (ED-1, CD68 positive cells), GAL-1 and ICAM-1 (intercellular adhesion molecule-1) staining was performed in 3 µm sections of paraffin-embedded kidneys. .. After an antigen-retrieval step using citrate buffer pH 6.0, the endogenous peroxide activity was blocked, and the sections were incubated overnight at 4 °C with a primary rabbit polyclonal anti-caspase-3 Ab (1:1000, 9662, Cell Signaling Technology, Danvers, MA, USA), anti-GAL-1 Ab (1:200, Zymed Laboratories, Cambridge, UK) or mouse monoclonal anti-ICAM-1 Ab (1:100, NB500–318, Novus Biological, Littleton, USA). .. For macrophage and nitrotyrosine detection, the sections were incubated for 1 h at room temperature with primary mouse monoclonal anti-CD68 Ab (1:500, MCA341R, Serotec, Oxford, UK) or anti-nitrotyrosine Ab (1:400, SC-32757, Santa Cruz Biotechnology, CA, USA).

    Incubation:

    Article Title: Pharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injury
    Article Snippet: Immunohistochemistry Caspase-3, nitrotyrosine, macrophages (ED-1, CD68 positive cells), GAL-1 and ICAM-1 (intercellular adhesion molecule-1) staining was performed in 3 µm sections of paraffin-embedded kidneys. .. After an antigen-retrieval step using citrate buffer pH 6.0, the endogenous peroxide activity was blocked, and the sections were incubated overnight at 4 °C with a primary rabbit polyclonal anti-caspase-3 Ab (1:1000, 9662, Cell Signaling Technology, Danvers, MA, USA), anti-GAL-1 Ab (1:200, Zymed Laboratories, Cambridge, UK) or mouse monoclonal anti-ICAM-1 Ab (1:100, NB500–318, Novus Biological, Littleton, USA). .. For macrophage and nitrotyrosine detection, the sections were incubated for 1 h at room temperature with primary mouse monoclonal anti-CD68 Ab (1:500, MCA341R, Serotec, Oxford, UK) or anti-nitrotyrosine Ab (1:400, SC-32757, Santa Cruz Biotechnology, CA, USA).

    Article Title: Edaravone, an ROS Scavenger, Ameliorates Photoreceptor Cell Death after Experimental Retinal Detachment
    Article Snippet: For immunostaining of macrophages, the sections were incubated with mouse anti-rat monocytes/macrophage (ED-1) monoclonal antibody (1:100; Millipore, Billerica, MA) overnight at 4°C. .. Alexa Flour 488-conjugated goat anti-mouse IgG (Invitrogen, Carlsbad, CA) was used as the secondary antibody and incubated at room temperature for 1 hour. .. Images of the retina were taken with an upright fluorescence microscope (DM RXA; Leica, Solms, Germany), and the number of ED-1-positive cells was counted.

    Immunofluorescence:

    Article Title: Macrophage-Derived Factors Stimulate Optic Nerve Regeneration
    Article Snippet: Immunohistochemistry to visualize GAP-43-positive axons was performed as described ( ) using an anti-GAP-43 antibody prepared in sheep [IgG fraction, 1:50,000 ( )] followed by a biotinylated secondary antibody, avidin–biotin complex (Vector Labs, Burlingame, CA), and diaminobenzidine enhanced with NiCl2 (Vector Labs). .. In cases in which GAP-43 was visualized by immunofluorescence, the primary antibody was diluted 1:2500, and the secondary antibody was a fluorescein-conjugated anti-sheep IgG made in donkey (1:500, Alexa Fluor 488; Molecular Probes, Eugene, OR). .. Reactive macrophages were visualized with the ED-1 monoclonal antibody (1:200 dilution; Serotec, Raleigh, NC) in all cases.

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    Thermo Fisher ed 1 positive macrophages
    Macrophage activation induces axon regeneration in the rat optic nerve. Sections through the retina ( a , c , e ) or the optic nerve ( b , d , f ) were stained with antibodies to detect GAP-43 ( a – f , green fluorescence) or the macrophage marker <t>ED-1</t> ( a , c , e , red fluorescence) 2 weeks after optic nerve surgery. a , GAP-43 is not detected in the GCL ( open arrowheads ) of animals that had received control PBS injections after optic nerve damage; ED-1 + macrophages are absent. b , Few GAP-43-positive axons extend past the injury site ( asterisk ) in the optic nerve. c , Zymosan injected into the vitreous the same day as nerve injury stimulates ED-1 + macrophages to infiltrate the eye and distribute near the GCL ( arrows ). GAP-43 expression is intense in RGC somata ( arrowheads ), and many GAP-43-positive axons extend into the distal optic nerve ( d ). e , Zymosan injections made 3 d after nerve injury result in high levels of GAP-43 in RGCs ( arrowheads ) and greater numbers of GAP-43-positive axons extending distal to the injury site ( f ). Scale bars: a , c , e , 250 μm; b , d , f , 200 μm.
    Ed 1 Positive Macrophages, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ed 1 positive macrophages/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ed 1 positive macrophages - by Bioz Stars, 2021-06
    95/100 stars
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    Macrophage activation induces axon regeneration in the rat optic nerve. Sections through the retina ( a , c , e ) or the optic nerve ( b , d , f ) were stained with antibodies to detect GAP-43 ( a – f , green fluorescence) or the macrophage marker ED-1 ( a , c , e , red fluorescence) 2 weeks after optic nerve surgery. a , GAP-43 is not detected in the GCL ( open arrowheads ) of animals that had received control PBS injections after optic nerve damage; ED-1 + macrophages are absent. b , Few GAP-43-positive axons extend past the injury site ( asterisk ) in the optic nerve. c , Zymosan injected into the vitreous the same day as nerve injury stimulates ED-1 + macrophages to infiltrate the eye and distribute near the GCL ( arrows ). GAP-43 expression is intense in RGC somata ( arrowheads ), and many GAP-43-positive axons extend into the distal optic nerve ( d ). e , Zymosan injections made 3 d after nerve injury result in high levels of GAP-43 in RGCs ( arrowheads ) and greater numbers of GAP-43-positive axons extending distal to the injury site ( f ). Scale bars: a , c , e , 250 μm; b , d , f , 200 μm.

    Journal: The Journal of Neuroscience

    Article Title: Macrophage-Derived Factors Stimulate Optic Nerve Regeneration

    doi: 10.1523/JNEUROSCI.23-06-02284.2003

    Figure Lengend Snippet: Macrophage activation induces axon regeneration in the rat optic nerve. Sections through the retina ( a , c , e ) or the optic nerve ( b , d , f ) were stained with antibodies to detect GAP-43 ( a – f , green fluorescence) or the macrophage marker ED-1 ( a , c , e , red fluorescence) 2 weeks after optic nerve surgery. a , GAP-43 is not detected in the GCL ( open arrowheads ) of animals that had received control PBS injections after optic nerve damage; ED-1 + macrophages are absent. b , Few GAP-43-positive axons extend past the injury site ( asterisk ) in the optic nerve. c , Zymosan injected into the vitreous the same day as nerve injury stimulates ED-1 + macrophages to infiltrate the eye and distribute near the GCL ( arrows ). GAP-43 expression is intense in RGC somata ( arrowheads ), and many GAP-43-positive axons extend into the distal optic nerve ( d ). e , Zymosan injections made 3 d after nerve injury result in high levels of GAP-43 in RGCs ( arrowheads ) and greater numbers of GAP-43-positive axons extending distal to the injury site ( f ). Scale bars: a , c , e , 250 μm; b , d , f , 200 μm.

    Article Snippet: Secondary antibodies conjugated to distinct fluorophores were used to visualize ED-1-positive macrophages (Texas Red-conjugated anti-mouse IgG made in goat, 1:500; Molecular Probes) and GAP-43-positive RGCs (Alexa Fluor 488-conjugated anti-sheep IgG made in donkey, 1:500; Molecular Probes) in the same sections.

    Techniques: Activation Assay, Staining, Fluorescence, Marker, Injection, Expressing

    Effect of Zymosan dosage on RGC survival. a , Zymosan injections, either on the day of nerve crush ( D0 ) or 3 d later ( D3 ), increased the number of TUJ1 + cells in the retina 3 weeks after a peripheral nerve graft. Zymosan resulted in more surviving TUJ1 + cells when injected at a low concentration (1.25 μg/μl) than at a high concentration (12.5 μg/μl). b , The higher dosage of Zymosan diminished retinal size. c , Normal retina (flat mounted). No macrophages appear in the vitreous or around the GCL, although some ED-1 + microglia are seen ( arrowhead ). d , Axotomy followed by a PN graft increases the number of ED-1 + monocytes ( arrow, arrowheads ) in the retina only slightly. e , Zymosan injections (1.25 μg/μl) result in accumulation of ED-1 + macrophages ( arrows ) in the vitreous and around the GCL. * p

    Journal: The Journal of Neuroscience

    Article Title: Macrophage-Derived Factors Stimulate Optic Nerve Regeneration

    doi: 10.1523/JNEUROSCI.23-06-02284.2003

    Figure Lengend Snippet: Effect of Zymosan dosage on RGC survival. a , Zymosan injections, either on the day of nerve crush ( D0 ) or 3 d later ( D3 ), increased the number of TUJ1 + cells in the retina 3 weeks after a peripheral nerve graft. Zymosan resulted in more surviving TUJ1 + cells when injected at a low concentration (1.25 μg/μl) than at a high concentration (12.5 μg/μl). b , The higher dosage of Zymosan diminished retinal size. c , Normal retina (flat mounted). No macrophages appear in the vitreous or around the GCL, although some ED-1 + microglia are seen ( arrowhead ). d , Axotomy followed by a PN graft increases the number of ED-1 + monocytes ( arrow, arrowheads ) in the retina only slightly. e , Zymosan injections (1.25 μg/μl) result in accumulation of ED-1 + macrophages ( arrows ) in the vitreous and around the GCL. * p

    Article Snippet: Secondary antibodies conjugated to distinct fluorophores were used to visualize ED-1-positive macrophages (Texas Red-conjugated anti-mouse IgG made in goat, 1:500; Molecular Probes) and GAP-43-positive RGCs (Alexa Fluor 488-conjugated anti-sheep IgG made in donkey, 1:500; Molecular Probes) in the same sections.

    Techniques: Injection, Concentration Assay