ecorv  (Thermo Fisher)


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    Name:
    EcoRI 10 U µL
    Description:
    5 G ↓A A T T C 3 3 C T T A A ↑G 5 Thermo Scientific EcoRI restriction enzyme recognizes G AATTC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    ER0271
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Cloning|Restriction Enzyme Cloning
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    Structured Review

    Thermo Fisher ecorv
    Restriction enzyme-digested fragments of the genomic <t>DNA</t> of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with <t>EcoRV;</t> EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.
    5 G ↓A A T T C 3 3 C T T A A ↑G 5 Thermo Scientific EcoRI restriction enzyme recognizes G AATTC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/ecorv/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Protective Effects of Bacteriophages against Aeromonas hydrophila Causing Motile Aeromonas Septicemia (MAS) in Striped Catfish"

    Article Title: Protective Effects of Bacteriophages against Aeromonas hydrophila Causing Motile Aeromonas Septicemia (MAS) in Striped Catfish

    Journal: Antibiotics

    doi: 10.3390/antibiotics7010016

    Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.
    Figure Legend Snippet: Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.

    Techniques Used: Marker

    2) Product Images from "Absence of XRCC4 and its paralogs in human cells reveal differences in outcomes for DNA repair and V(D)J recombination"

    Article Title: Absence of XRCC4 and its paralogs in human cells reveal differences in outcomes for DNA repair and V(D)J recombination

    Journal: DNA repair

    doi: 10.1016/j.dnarep.2019.102738

    Confirmation of microhomology-mediated end-joining in XRCC4-deficient cells.(A) A microhomology-directed NHEJ (aEJ) biased reporter substrate plasmid has been engineered so that digestion with Eco47III and EcoRV yields a blunt-ended linear substrate with 6-bp repeats (boxes) at each end. Repair of this plasmid by C-NHEJ joining will result in the retention of both repeats while aEJ should yield a single repeat, which conforms to a BstXI restriction enzyme recognition site. (B) Experimental strategy for analysis of repair events produced in transfected cells. The recovered plasmids are subjected to PCR amplification using primers flanking the repair junction. These ~ 180 bp PCR products are then subjected to BstXI restriction enzyme digestion that will yield 120 bp and 60 bp products if successfully cleaved. The restriction fragments are visualized with SYBR Gold following polyacrylamide gel electrophoresis. (C) The results of one such experiment. (D) Two independent experiments including the one shown in (C) were quantitated using ImageJ software and averaged.
    Figure Legend Snippet: Confirmation of microhomology-mediated end-joining in XRCC4-deficient cells.(A) A microhomology-directed NHEJ (aEJ) biased reporter substrate plasmid has been engineered so that digestion with Eco47III and EcoRV yields a blunt-ended linear substrate with 6-bp repeats (boxes) at each end. Repair of this plasmid by C-NHEJ joining will result in the retention of both repeats while aEJ should yield a single repeat, which conforms to a BstXI restriction enzyme recognition site. (B) Experimental strategy for analysis of repair events produced in transfected cells. The recovered plasmids are subjected to PCR amplification using primers flanking the repair junction. These ~ 180 bp PCR products are then subjected to BstXI restriction enzyme digestion that will yield 120 bp and 60 bp products if successfully cleaved. The restriction fragments are visualized with SYBR Gold following polyacrylamide gel electrophoresis. (C) The results of one such experiment. (D) Two independent experiments including the one shown in (C) were quantitated using ImageJ software and averaged.

    Techniques Used: Non-Homologous End Joining, Plasmid Preparation, Produced, Transfection, Polymerase Chain Reaction, Amplification, Polyacrylamide Gel Electrophoresis, Software

    3) Product Images from "Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172"

    Article Title: Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

    Journal: Molecular Microbiology

    doi: 10.1111/j.1365-2958.2010.07474.x

    DNA inversion of the UU172 element in U. parvum serotype 3 clonal variant V892-K2. A and B. (A) Schematic illustration of the DNA inversion event and (B) Southern blot analysis of V892-K2 before (P0) and after (P3) selective pressure with Pab α-U172C. Genomic DNA was digested with BglII and EcoRV and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots. C. PCR analysis of subclones A and B with primer pairs 17/20, 17/22, 23/24 and 23/25 (see Table S3 ).
    Figure Legend Snippet: DNA inversion of the UU172 element in U. parvum serotype 3 clonal variant V892-K2. A and B. (A) Schematic illustration of the DNA inversion event and (B) Southern blot analysis of V892-K2 before (P0) and after (P3) selective pressure with Pab α-U172C. Genomic DNA was digested with BglII and EcoRV and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots. C. PCR analysis of subclones A and B with primer pairs 17/20, 17/22, 23/24 and 23/25 (see Table S3 ).

    Techniques Used: Variant Assay, Southern Blot, Polymerase Chain Reaction

    4) Product Images from "Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments"

    Article Title: Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-30371-3

    Restriction enzyme profiles of representative Lactococcus phages analysed in this study. DNA samples were cut overnight with EcoRV at 37 °C and treated with 50% formamide to dissociate cos ends prior to electrophoresis. M: 1-kb DNA Ladder (Fermentas). Unique phage DNA restriction patterns are marked by an asterisk (*).
    Figure Legend Snippet: Restriction enzyme profiles of representative Lactococcus phages analysed in this study. DNA samples were cut overnight with EcoRV at 37 °C and treated with 50% formamide to dissociate cos ends prior to electrophoresis. M: 1-kb DNA Ladder (Fermentas). Unique phage DNA restriction patterns are marked by an asterisk (*).

    Techniques Used: Electrophoresis

    5) Product Images from "Evolution of long centromeres in fire ants"

    Article Title: Evolution of long centromeres in fire ants

    Journal: BMC Evolutionary Biology

    doi: 10.1186/s12862-016-0760-7

    a Localization of the first and the second most abundant satellites on the metaphase chromosomes of S. invicta and S. geminata . FISH analysis on haploid cells combining the CenSol probe (green) with the second most abundant satellite ( Solmin , blue); chromosomes counterstained with DAPI (gray). b Summary of the length distributions of the CenSol repeat in the S. invicta and S. geminata draft genomes. BLASTN hits of the consensus CenSol sequence against the S. invicta and S. geminata genomes were binned by sequence length. The 109 bp unit was the dominant repeat length for both ants. c Sequence logos generated with 10,469 and 5423 unique 109 bp CenSol sequences from S. invicta and S. geminata , respectively. The height of each letter is proportional to the frequency of four nucleotides, adenine (A), thymine (T), guanine (G), and cytosine (C). The total height of each stack, measured in bits, is related to the binding energy [ 57 ]. EcoRV and BsaAI restriction sites are indicated with arrowheads. The BsaAI in parenthesis indicates a less common polymorphic cutting site in the CenSol monomers. The locations of the A1repV1 primers are shown
    Figure Legend Snippet: a Localization of the first and the second most abundant satellites on the metaphase chromosomes of S. invicta and S. geminata . FISH analysis on haploid cells combining the CenSol probe (green) with the second most abundant satellite ( Solmin , blue); chromosomes counterstained with DAPI (gray). b Summary of the length distributions of the CenSol repeat in the S. invicta and S. geminata draft genomes. BLASTN hits of the consensus CenSol sequence against the S. invicta and S. geminata genomes were binned by sequence length. The 109 bp unit was the dominant repeat length for both ants. c Sequence logos generated with 10,469 and 5423 unique 109 bp CenSol sequences from S. invicta and S. geminata , respectively. The height of each letter is proportional to the frequency of four nucleotides, adenine (A), thymine (T), guanine (G), and cytosine (C). The total height of each stack, measured in bits, is related to the binding energy [ 57 ]. EcoRV and BsaAI restriction sites are indicated with arrowheads. The BsaAI in parenthesis indicates a less common polymorphic cutting site in the CenSol monomers. The locations of the A1repV1 primers are shown

    Techniques Used: Fluorescence In Situ Hybridization, Sequencing, Generated, Binding Assay

    6) Product Images from "Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods"

    Article Title: Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods

    Journal: Journal of Food Science and Technology

    doi: 10.1007/s13197-017-2964-2

    Phage vB_SflS-ISF001 genomic DNA size. Lane M: X-large DNA marker (SinaClon, Iran); lane 1: Untreated genomic DNA; lane 2: EcoRI; lane 3: EcoRV; lane 4: HindIII; lane 5: BamHI
    Figure Legend Snippet: Phage vB_SflS-ISF001 genomic DNA size. Lane M: X-large DNA marker (SinaClon, Iran); lane 1: Untreated genomic DNA; lane 2: EcoRI; lane 3: EcoRV; lane 4: HindIII; lane 5: BamHI

    Techniques Used: Marker

    7) Product Images from "Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment"

    Article Title: Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2021.619323

    (Left side) The electron micrograph of phages vB _SflM_004 (A,B) , vB_SdyM_006 (C,D) , and vB_SsoS_008 (E,F) . The samples were negatively stained with 2% phosphotungstic acid (PTA). Scale bars 100nm. (Right side) The DNA fingerprinting analysis of the genomic DNA of phage vB _SflM_004, vB_SdyM_006, and vB_SsoS_008. The genome was digested with EcoRI (line1), EcoRV (line2), and HindIII (line3). M line represents the DNA marker.
    Figure Legend Snippet: (Left side) The electron micrograph of phages vB _SflM_004 (A,B) , vB_SdyM_006 (C,D) , and vB_SsoS_008 (E,F) . The samples were negatively stained with 2% phosphotungstic acid (PTA). Scale bars 100nm. (Right side) The DNA fingerprinting analysis of the genomic DNA of phage vB _SflM_004, vB_SdyM_006, and vB_SsoS_008. The genome was digested with EcoRI (line1), EcoRV (line2), and HindIII (line3). M line represents the DNA marker.

    Techniques Used: Staining, DNA Profiling, Marker

    8) Product Images from "Mechanisms of Igf2 inhibition in thymic epithelial cells infected by coxsackievirus CV-B4"

    Article Title: Mechanisms of Igf2 inhibition in thymic epithelial cells infected by coxsackievirus CV-B4

    Journal: bioRxiv

    doi: 10.1101/2020.01.10.902684

    Effect of CV-B4 E2 on Igf2 P3 promoter activity in MTE4-14 and on Igf2 transcript stability. (A) Sequence of the murine Igf2 P3 promoter sequence (−168/+175) https://epd.epfl.ch . The restriction site NheI and EcoRV are indicated above in italic. The transcription start site is represented by an arrow at +1. (B) Nanoluciferase relative activity of Igf2 P3 promoter (−168/+175) after 1 (left panel) and 2 days P.I. (right panel). Analysis was realized as described in methods. Mean of relative dual-luciferase activity normalized to mock are represented ± SEM. (C) mRNA half-life of Igf2 V3 transcripts in CV-B4 E2 MOI = 0.05 or mock uninfected cells at day 2 P.I. followed by 2-10 hours of treatment with actinomycin D ( n = 4), vehicle control was used for data normalization for each time point. (B-C) ratio paired t test, *** p
    Figure Legend Snippet: Effect of CV-B4 E2 on Igf2 P3 promoter activity in MTE4-14 and on Igf2 transcript stability. (A) Sequence of the murine Igf2 P3 promoter sequence (−168/+175) https://epd.epfl.ch . The restriction site NheI and EcoRV are indicated above in italic. The transcription start site is represented by an arrow at +1. (B) Nanoluciferase relative activity of Igf2 P3 promoter (−168/+175) after 1 (left panel) and 2 days P.I. (right panel). Analysis was realized as described in methods. Mean of relative dual-luciferase activity normalized to mock are represented ± SEM. (C) mRNA half-life of Igf2 V3 transcripts in CV-B4 E2 MOI = 0.05 or mock uninfected cells at day 2 P.I. followed by 2-10 hours of treatment with actinomycin D ( n = 4), vehicle control was used for data normalization for each time point. (B-C) ratio paired t test, *** p

    Techniques Used: Activity Assay, Sequencing, Luciferase

    Related Articles

    Plasmid Preparation:

    Article Title: The ETS Transcription Factor ESE-1 Transforms MCF-12A Human Mammary Epithelial Cells via a Novel Cytoplasmic Mechanism
    Article Snippet: The ESE-1 coding sequence in the adenovirus genome was verified by dideoxy sequencing at the UCHSC Cancer Center DNA Sequencing Core Facility. .. An EcoRI ESE-1 cDNA fragment excised from pCR2.1-ESE-1 ( ) was ligated to EcoRI-cut plasmid pEGFP-C3 (Invitrogen, Inc., Carlsbad, Calif.), in frame and downstream of the green fluorescent protein (GFP) gene (see Fig. ), to produce pEGFP-ESE-1. .. Internal deletions in GFP-ESE-1 were generated by using two PCRs, one generating the amino-terminal regions and one generating the carboxy-terminal regions flanking each internal deletion.

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Targeting Activation Induced Cytidine Deaminase Overcome Tumor Evasion of Immunotherapy by Cytotoxic T Lymphocytes
    Article Snippet: ApaI and EcoRI restriction sites were added to the AID targeting sequence (5'-AATTGTTGTTCCTACGCTACA-3') at 5'- and 3'-ends. .. We then ligated the DNA nucleotide sequence into the ApaI and EcoRI sites of pSilencer™1.0 vector (U6 siRNA Expression Vector, Ambion) and used it to co-transfect with a selection vector containing the Neo gene into J558 tumor cells. .. For control, we used empty targeting vectors and Neo vector co-transfected J558 cells.

    Article Title: Design, Construction and Immunogenicity Assessment of pEGFP-N1-KMP11-GP96 (Fusion) as a DNA Vaccine Candidate against Leishmania major Infection in BALB/c Mice
    Article Snippet: For confirmation, PCR amplifications were performed in these colonies using primers specific for KMP-11and NT-GP96 genes and colonies containing the recombinant plasmid were selected. .. Recombinant plasmids were extracted by Vivantis plasmid extraction kit and digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes (Fermentas Co.®) for digestion confirmation. .. Subcloning of KMP-11and NT-GP96 in pEGFP-N1expression vector pJET- KMP-11, pJET- NT-GP96 and pEGFP-N1 were digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes and the purified gene fragment of KMP-11and NT-GP96 KMP-11 ligated into digested pEGFPN1expression vector by using of T4 DNA ligase enzyme.

    Ligation:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Polymerase Chain Reaction:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Clone Assay:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Sequencing:

    Article Title: Targeting Activation Induced Cytidine Deaminase Overcome Tumor Evasion of Immunotherapy by Cytotoxic T Lymphocytes
    Article Snippet: ApaI and EcoRI restriction sites were added to the AID targeting sequence (5'-AATTGTTGTTCCTACGCTACA-3') at 5'- and 3'-ends. .. We then ligated the DNA nucleotide sequence into the ApaI and EcoRI sites of pSilencer™1.0 vector (U6 siRNA Expression Vector, Ambion) and used it to co-transfect with a selection vector containing the Neo gene into J558 tumor cells. .. For control, we used empty targeting vectors and Neo vector co-transfected J558 cells.

    Expressing:

    Article Title: Targeting Activation Induced Cytidine Deaminase Overcome Tumor Evasion of Immunotherapy by Cytotoxic T Lymphocytes
    Article Snippet: ApaI and EcoRI restriction sites were added to the AID targeting sequence (5'-AATTGTTGTTCCTACGCTACA-3') at 5'- and 3'-ends. .. We then ligated the DNA nucleotide sequence into the ApaI and EcoRI sites of pSilencer™1.0 vector (U6 siRNA Expression Vector, Ambion) and used it to co-transfect with a selection vector containing the Neo gene into J558 tumor cells. .. For control, we used empty targeting vectors and Neo vector co-transfected J558 cells.

    Selection:

    Article Title: Targeting Activation Induced Cytidine Deaminase Overcome Tumor Evasion of Immunotherapy by Cytotoxic T Lymphocytes
    Article Snippet: ApaI and EcoRI restriction sites were added to the AID targeting sequence (5'-AATTGTTGTTCCTACGCTACA-3') at 5'- and 3'-ends. .. We then ligated the DNA nucleotide sequence into the ApaI and EcoRI sites of pSilencer™1.0 vector (U6 siRNA Expression Vector, Ambion) and used it to co-transfect with a selection vector containing the Neo gene into J558 tumor cells. .. For control, we used empty targeting vectors and Neo vector co-transfected J558 cells.

    Recombinant:

    Article Title: Design, Construction and Immunogenicity Assessment of pEGFP-N1-KMP11-GP96 (Fusion) as a DNA Vaccine Candidate against Leishmania major Infection in BALB/c Mice
    Article Snippet: For confirmation, PCR amplifications were performed in these colonies using primers specific for KMP-11and NT-GP96 genes and colonies containing the recombinant plasmid were selected. .. Recombinant plasmids were extracted by Vivantis plasmid extraction kit and digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes (Fermentas Co.®) for digestion confirmation. .. Subcloning of KMP-11and NT-GP96 in pEGFP-N1expression vector pJET- KMP-11, pJET- NT-GP96 and pEGFP-N1 were digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes and the purified gene fragment of KMP-11and NT-GP96 KMP-11 ligated into digested pEGFPN1expression vector by using of T4 DNA ligase enzyme.

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  • 99
    Thermo Fisher ecorv
    Restriction enzyme-digested fragments of the genomic <t>DNA</t> of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with <t>EcoRV;</t> EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.
    Ecorv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

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    Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.

    Journal: Antibiotics

    Article Title: Protective Effects of Bacteriophages against Aeromonas hydrophila Causing Motile Aeromonas Septicemia (MAS) in Striped Catfish

    doi: 10.3390/antibiotics7010016

    Figure Lengend Snippet: Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.

    Article Snippet: The genomic DNA phages were digested using the restriction enzymes: EcoRV, EcoRI, Ncol, SalI, MspI, XmnI, and KpnI, as per the manufacturer’s instruction (ThermoFisher Scientific).

    Techniques: Marker

    Restriction enzyme profiles of representative Lactococcus phages analysed in this study. DNA samples were cut overnight with EcoRV at 37 °C and treated with 50% formamide to dissociate cos ends prior to electrophoresis. M: 1-kb DNA Ladder (Fermentas). Unique phage DNA restriction patterns are marked by an asterisk (*).

    Journal: Scientific Reports

    Article Title: Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments

    doi: 10.1038/s41598-018-30371-3

    Figure Lengend Snippet: Restriction enzyme profiles of representative Lactococcus phages analysed in this study. DNA samples were cut overnight with EcoRV at 37 °C and treated with 50% formamide to dissociate cos ends prior to electrophoresis. M: 1-kb DNA Ladder (Fermentas). Unique phage DNA restriction patterns are marked by an asterisk (*).

    Article Snippet: Essentially, the genomic DNA of 90 phage isolates was cut separately with EcoRI , EcoRV or HindIII restriction endonucleases (Fermentas) overnight at 37 °C as recommended by the manufacturer.

    Techniques: Electrophoresis