Structured Review

Thermo Fisher ecorv
Effect of CV-B4 E2 on Igf2 P3 promoter activity in MTE4-14 and on Igf2 transcript stability. (A) Sequence of the murine Igf2 P3 promoter sequence (−168/+175) https://epd.epfl.ch . The restriction site <t>NheI</t> and <t>EcoRV</t> are indicated above in italic. The transcription start site is represented by an arrow at +1. (B) Nanoluciferase relative activity of Igf2 P3 promoter (−168/+175) after 1 (left panel) and 2 days P.I. (right panel). Analysis was realized as described in methods. Mean of relative dual-luciferase activity normalized to mock are represented ± SEM. (C) mRNA half-life of Igf2 V3 transcripts in CV-B4 E2 MOI = 0.05 or mock uninfected cells at day 2 P.I. followed by 2-10 hours of treatment with actinomycin D ( n = 4), vehicle control was used for data normalization for each time point. (B-C) ratio paired t test, *** p
Ecorv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Mechanisms of Igf2 inhibition in thymic epithelial cells infected by coxsackievirus CV-B4"

Article Title: Mechanisms of Igf2 inhibition in thymic epithelial cells infected by coxsackievirus CV-B4

Journal: bioRxiv

doi: 10.1101/2020.01.10.902684

Effect of CV-B4 E2 on Igf2 P3 promoter activity in MTE4-14 and on Igf2 transcript stability. (A) Sequence of the murine Igf2 P3 promoter sequence (−168/+175) https://epd.epfl.ch . The restriction site NheI and EcoRV are indicated above in italic. The transcription start site is represented by an arrow at +1. (B) Nanoluciferase relative activity of Igf2 P3 promoter (−168/+175) after 1 (left panel) and 2 days P.I. (right panel). Analysis was realized as described in methods. Mean of relative dual-luciferase activity normalized to mock are represented ± SEM. (C) mRNA half-life of Igf2 V3 transcripts in CV-B4 E2 MOI = 0.05 or mock uninfected cells at day 2 P.I. followed by 2-10 hours of treatment with actinomycin D ( n = 4), vehicle control was used for data normalization for each time point. (B-C) ratio paired t test, *** p
Figure Legend Snippet: Effect of CV-B4 E2 on Igf2 P3 promoter activity in MTE4-14 and on Igf2 transcript stability. (A) Sequence of the murine Igf2 P3 promoter sequence (−168/+175) https://epd.epfl.ch . The restriction site NheI and EcoRV are indicated above in italic. The transcription start site is represented by an arrow at +1. (B) Nanoluciferase relative activity of Igf2 P3 promoter (−168/+175) after 1 (left panel) and 2 days P.I. (right panel). Analysis was realized as described in methods. Mean of relative dual-luciferase activity normalized to mock are represented ± SEM. (C) mRNA half-life of Igf2 V3 transcripts in CV-B4 E2 MOI = 0.05 or mock uninfected cells at day 2 P.I. followed by 2-10 hours of treatment with actinomycin D ( n = 4), vehicle control was used for data normalization for each time point. (B-C) ratio paired t test, *** p

Techniques Used: Activity Assay, Sequencing, Luciferase

2) Product Images from "Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments"

Article Title: Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments

Journal: Scientific Reports

doi: 10.1038/s41598-018-30371-3

Restriction enzyme profiles of representative Lactococcus phages analysed in this study. DNA samples were cut overnight with EcoRV at 37 °C and treated with 50% formamide to dissociate cos ends prior to electrophoresis. M: 1-kb DNA Ladder (Fermentas). Unique phage DNA restriction patterns are marked by an asterisk (*).
Figure Legend Snippet: Restriction enzyme profiles of representative Lactococcus phages analysed in this study. DNA samples were cut overnight with EcoRV at 37 °C and treated with 50% formamide to dissociate cos ends prior to electrophoresis. M: 1-kb DNA Ladder (Fermentas). Unique phage DNA restriction patterns are marked by an asterisk (*).

Techniques Used: Electrophoresis

3) Product Images from "Protective Effects of Bacteriophages against Aeromonas hydrophila Causing Motile Aeromonas Septicemia (MAS) in Striped Catfish"

Article Title: Protective Effects of Bacteriophages against Aeromonas hydrophila Causing Motile Aeromonas Septicemia (MAS) in Striped Catfish

Journal: Antibiotics

doi: 10.3390/antibiotics7010016

Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.
Figure Legend Snippet: Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.

Techniques Used: Marker

4) Product Images from "Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172"

Article Title: Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

Journal: Molecular Microbiology

doi: 10.1111/j.1365-2958.2010.07474.x

DNA inversion of the UU172 element in U. parvum serotype 3 clonal variant V892-K2. A and B. (A) Schematic illustration of the DNA inversion event and (B) Southern blot analysis of V892-K2 before (P0) and after (P3) selective pressure with Pab α-U172C. Genomic DNA was digested with BglII and EcoRV and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots. C. PCR analysis of subclones A and B with primer pairs 17/20, 17/22, 23/24 and 23/25 (see Table S3 ).
Figure Legend Snippet: DNA inversion of the UU172 element in U. parvum serotype 3 clonal variant V892-K2. A and B. (A) Schematic illustration of the DNA inversion event and (B) Southern blot analysis of V892-K2 before (P0) and after (P3) selective pressure with Pab α-U172C. Genomic DNA was digested with BglII and EcoRV and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots. C. PCR analysis of subclones A and B with primer pairs 17/20, 17/22, 23/24 and 23/25 (see Table S3 ).

Techniques Used: Variant Assay, Southern Blot, Polymerase Chain Reaction

5) Product Images from "Evolution of long centromeres in fire ants"

Article Title: Evolution of long centromeres in fire ants

Journal: BMC Evolutionary Biology

doi: 10.1186/s12862-016-0760-7

a Localization of the first and the second most abundant satellites on the metaphase chromosomes of S. invicta and S. geminata . FISH analysis on haploid cells combining the CenSol probe (green) with the second most abundant satellite ( Solmin , blue); chromosomes counterstained with DAPI (gray). b Summary of the length distributions of the CenSol repeat in the S. invicta and S. geminata draft genomes. BLASTN hits of the consensus CenSol sequence against the S. invicta and S. geminata genomes were binned by sequence length. The 109 bp unit was the dominant repeat length for both ants. c Sequence logos generated with 10,469 and 5423 unique 109 bp CenSol sequences from S. invicta and S. geminata , respectively. The height of each letter is proportional to the frequency of four nucleotides, adenine (A), thymine (T), guanine (G), and cytosine (C). The total height of each stack, measured in bits, is related to the binding energy [ 57 ]. EcoRV and BsaAI restriction sites are indicated with arrowheads. The BsaAI in parenthesis indicates a less common polymorphic cutting site in the CenSol monomers. The locations of the A1repV1 primers are shown
Figure Legend Snippet: a Localization of the first and the second most abundant satellites on the metaphase chromosomes of S. invicta and S. geminata . FISH analysis on haploid cells combining the CenSol probe (green) with the second most abundant satellite ( Solmin , blue); chromosomes counterstained with DAPI (gray). b Summary of the length distributions of the CenSol repeat in the S. invicta and S. geminata draft genomes. BLASTN hits of the consensus CenSol sequence against the S. invicta and S. geminata genomes were binned by sequence length. The 109 bp unit was the dominant repeat length for both ants. c Sequence logos generated with 10,469 and 5423 unique 109 bp CenSol sequences from S. invicta and S. geminata , respectively. The height of each letter is proportional to the frequency of four nucleotides, adenine (A), thymine (T), guanine (G), and cytosine (C). The total height of each stack, measured in bits, is related to the binding energy [ 57 ]. EcoRV and BsaAI restriction sites are indicated with arrowheads. The BsaAI in parenthesis indicates a less common polymorphic cutting site in the CenSol monomers. The locations of the A1repV1 primers are shown

Techniques Used: Fluorescence In Situ Hybridization, Sequencing, Generated, Binding Assay

6) Product Images from "Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods"

Article Title: Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods

Journal: Journal of Food Science and Technology

doi: 10.1007/s13197-017-2964-2

Phage vB_SflS-ISF001 genomic DNA size. Lane M: X-large DNA marker (SinaClon, Iran); lane 1: Untreated genomic DNA; lane 2: EcoRI; lane 3: EcoRV; lane 4: HindIII; lane 5: BamHI
Figure Legend Snippet: Phage vB_SflS-ISF001 genomic DNA size. Lane M: X-large DNA marker (SinaClon, Iran); lane 1: Untreated genomic DNA; lane 2: EcoRI; lane 3: EcoRV; lane 4: HindIII; lane 5: BamHI

Techniques Used: Marker

Related Articles

Agarose Gel Electrophoresis:

Article Title: Development of Low Phytate Rice by RNAi Mediated Seed-Specific Silencing of Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase Gene (IPK1)
Article Snippet: .. Genomic DNA (10 µg) was digested separately with EcoR I and Hind III (Fermentas), separated on a 1% agarose gel, and transferred to a nylon membrane (Hybond N+, Amersham, GE Healthcare). .. The RGA2 intron (PCR product) labeled with [α-32 P]-dCTP radioisotope (BARC, India) using the Decalabel DNA labeling kit (Fermentas) was used as the probe for hybridization.

Synthesized:

Article Title: Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2
Article Snippet: .. The resulting modified genes were codon- and RNA-optimized and synthesized by DNA synthesis service (GeneArt, Thermo Fisher Scientific, Washington, NC, USA), digested with EcoR I and Not I and individually subcloned into the EcoR I-Not I site of pVax1 (Invitrogen Thermo Fisher Scientific, Grand Island, NY, USA) to construct heavy chain (pVax1-4D5-mIgG1-H or pVax1-4D5-mIgG2a-H) and light chain (pVax1-4D5-mIgG-L) expression plasmids, respectively. .. Purified plasmid DNA was formulated in water for administration into mice.

Construct:

Article Title: Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2
Article Snippet: .. The resulting modified genes were codon- and RNA-optimized and synthesized by DNA synthesis service (GeneArt, Thermo Fisher Scientific, Washington, NC, USA), digested with EcoR I and Not I and individually subcloned into the EcoR I-Not I site of pVax1 (Invitrogen Thermo Fisher Scientific, Grand Island, NY, USA) to construct heavy chain (pVax1-4D5-mIgG1-H or pVax1-4D5-mIgG2a-H) and light chain (pVax1-4D5-mIgG-L) expression plasmids, respectively. .. Purified plasmid DNA was formulated in water for administration into mice.

Expressing:

Article Title: Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2
Article Snippet: .. The resulting modified genes were codon- and RNA-optimized and synthesized by DNA synthesis service (GeneArt, Thermo Fisher Scientific, Washington, NC, USA), digested with EcoR I and Not I and individually subcloned into the EcoR I-Not I site of pVax1 (Invitrogen Thermo Fisher Scientific, Grand Island, NY, USA) to construct heavy chain (pVax1-4D5-mIgG1-H or pVax1-4D5-mIgG2a-H) and light chain (pVax1-4D5-mIgG-L) expression plasmids, respectively. .. Purified plasmid DNA was formulated in water for administration into mice.

Modification:

Article Title: Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2
Article Snippet: .. The resulting modified genes were codon- and RNA-optimized and synthesized by DNA synthesis service (GeneArt, Thermo Fisher Scientific, Washington, NC, USA), digested with EcoR I and Not I and individually subcloned into the EcoR I-Not I site of pVax1 (Invitrogen Thermo Fisher Scientific, Grand Island, NY, USA) to construct heavy chain (pVax1-4D5-mIgG1-H or pVax1-4D5-mIgG2a-H) and light chain (pVax1-4D5-mIgG-L) expression plasmids, respectively. .. Purified plasmid DNA was formulated in water for administration into mice.

DNA Synthesis:

Article Title: Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2
Article Snippet: .. The resulting modified genes were codon- and RNA-optimized and synthesized by DNA synthesis service (GeneArt, Thermo Fisher Scientific, Washington, NC, USA), digested with EcoR I and Not I and individually subcloned into the EcoR I-Not I site of pVax1 (Invitrogen Thermo Fisher Scientific, Grand Island, NY, USA) to construct heavy chain (pVax1-4D5-mIgG1-H or pVax1-4D5-mIgG2a-H) and light chain (pVax1-4D5-mIgG-L) expression plasmids, respectively. .. Purified plasmid DNA was formulated in water for administration into mice.

Recombinant:

Article Title: Induction of mucosal immune responses and protection of cattle against direct-contact challenge by intranasal delivery with foot-and-mouth disease virus antigen mediated by nanoparticles
Article Snippet: .. P12A was digested using BamH I and EcoR I, and 3C was digested with EcoR I and Xbal I, and fragments were subcloned into the corresponding sites of pcDNA3.1(+) (Invitrogen® ; Life Technologies) using T4 DNA ligase to generate recombinant plasmids pcDNA3.1/P12A3C. .. The sequence accuracy of recombinant plasmids was authenticated by specific polymerase chain reaction (PCR), double digestion, and DNA sequencing.

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  • 93
    Thermo Fisher eco32i
    Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), <t>Eco32I</t> (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.
    Eco32i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eco32i/product/Thermo Fisher
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      Buy from Supplier

    97
    Thermo Fisher ecorv
    Restriction enzyme-digested fragments of the genomic <t>DNA</t> of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with <t>EcoRV;</t> EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.
    Ecorv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv/product/Thermo Fisher
    Average 97 stars, based on 189 article reviews
    Price from $9.99 to $1999.99
    ecorv - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), Eco32I (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.

    Journal: PLoS ONE

    Article Title: Two Inducible Prophages of an Antarctic Pseudomonas sp. ANT_H14 Use the Same Capsid for Packaging Their Genomes – Characterization of a Novel Phage Helper-Satellite System

    doi: 10.1371/journal.pone.0158889

    Figure Lengend Snippet: Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), Eco32I (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.

    Article Snippet: The test for the presence of cohesive ends of the phage genome was performed as previously described [ ], using the following REases: HindIII, SalI, EcoRI, Eco32I and PstI (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Purification, Marker

    Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, Eco32I, HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler

    Journal: Journal of Virology

    Article Title: Molecular Characterization of a Novel Temperate Sinorhizobium Bacteriophage, ФLM21, Encoding DNA Methyltransferase with CcrM-Like Specificity

    doi: 10.1128/JVI.01875-14

    Figure Lengend Snippet: Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, Eco32I, HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler

    Article Snippet: The test for the presence of cohesive ends of the phage genome was performed as previously described , using the restriction enzymes HindIII, XhoI, Eco32I, SalI, and SmaI (Thermo Scientific).

    Techniques: In Vitro, Methylation, Generated

    Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.

    Journal: Antibiotics

    Article Title: Protective Effects of Bacteriophages against Aeromonas hydrophila Causing Motile Aeromonas Septicemia (MAS) in Striped Catfish

    doi: 10.3390/antibiotics7010016

    Figure Lengend Snippet: Restriction enzyme-digested fragments of the genomic DNA of A. hydrophila -phage 2. Footnote: Lane M: 1kb Plus Opti-DNA Marker (ABM, Canada); Lane L1: genomic DNA of Φ2; Lanes L2–L8: genomic DNA of Φ2 digested with EcoRV; EcoRI; Ncol; SalI; MspI; XmnI; KpnI, restriction enzymes respectively.

    Article Snippet: The genomic DNA phages were digested using the restriction enzymes: EcoRV, EcoRI, Ncol, SalI, MspI, XmnI, and KpnI, as per the manufacturer’s instruction (ThermoFisher Scientific).

    Techniques: Marker

    Phage vB_SflS-ISF001 genomic DNA size. Lane M: X-large DNA marker (SinaClon, Iran); lane 1: Untreated genomic DNA; lane 2: EcoRI; lane 3: EcoRV; lane 4: HindIII; lane 5: BamHI

    Journal: Journal of Food Science and Technology

    Article Title: Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods

    doi: 10.1007/s13197-017-2964-2

    Figure Lengend Snippet: Phage vB_SflS-ISF001 genomic DNA size. Lane M: X-large DNA marker (SinaClon, Iran); lane 1: Untreated genomic DNA; lane 2: EcoRI; lane 3: EcoRV; lane 4: HindIII; lane 5: BamHI

    Article Snippet: Phage’s nucleic acid was treated with the restriction enzymes including EcoRI , EcoRV , HindIII and BamHI (Thermo Fisher Scientific, US).

    Techniques: Marker