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Promega ecorv
Southern blot analysis of the transgenic chickens. Genomic <t>DNA</t> samples were extracted from whole blood samples from wild-type and transgenic chickens (G1 and G2), digested with <t>EcoRV,</t> electrophoresed, blotted, and hybridized with a probe for the 3D8 scFv gene. M, DIG-labeled DNA marker; lane 1, wild-type chicken; lane 2, P-54; lane 3, P-54 offspring; lane 4, N-82; lane 5, N-95; lane 6, N-165; lane 7, N-183; lane 8, P-196; lane 9, N-238.
Ecorv, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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1) Product Images from "Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission"

Article Title: Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission

Journal: Scientific Reports

doi: 10.1038/s41598-017-05270-8

Southern blot analysis of the transgenic chickens. Genomic DNA samples were extracted from whole blood samples from wild-type and transgenic chickens (G1 and G2), digested with EcoRV, electrophoresed, blotted, and hybridized with a probe for the 3D8 scFv gene. M, DIG-labeled DNA marker; lane 1, wild-type chicken; lane 2, P-54; lane 3, P-54 offspring; lane 4, N-82; lane 5, N-95; lane 6, N-165; lane 7, N-183; lane 8, P-196; lane 9, N-238.
Figure Legend Snippet: Southern blot analysis of the transgenic chickens. Genomic DNA samples were extracted from whole blood samples from wild-type and transgenic chickens (G1 and G2), digested with EcoRV, electrophoresed, blotted, and hybridized with a probe for the 3D8 scFv gene. M, DIG-labeled DNA marker; lane 1, wild-type chicken; lane 2, P-54; lane 3, P-54 offspring; lane 4, N-82; lane 5, N-95; lane 6, N-165; lane 7, N-183; lane 8, P-196; lane 9, N-238.

Techniques Used: Southern Blot, Transgenic Assay, Labeling, Marker

Related Articles

Construct:

Article Title: Heterogeneous Nuclear Ribonucleoprotein A2 Is a Common Transcriptional Coactivator in the Nuclear Transcription Response to Mitochondrial Respiratory Stress
Article Snippet: The cRel , C/EBP δ, NFAT , and CREB genes were cloned into the pCMV4 expression vector. hnRNP A2 cDNA was also subcloned from pET28a (+) vector into pCI for transfections of C2C12 cells. .. The gal4 fusion constructs were generated by cloning the full-length (1-342 aa) and the deletion constructs (1-180 aa, 90-242 aa, 178-342 aa, and 240-342 aa) in frame into the EcoRV and HindIII sites of the pBIND gal4 dbd (Checkmate Mammalian 2-hybrid system, Promega). .. Murine C2C12 skeletal myoblasts (CRL1772; American Type Culture Collection, Manassas, VA) were grown in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum and 0.1% gentamicin. mtDNA-depleted clones containing ∼80% reduced mtDNA contents were generated as described previously ( ) and grown in the presence of 1 mM sodium pyruvate and 50 μg/ml uridine.

Generated:

Article Title: Heterogeneous Nuclear Ribonucleoprotein A2 Is a Common Transcriptional Coactivator in the Nuclear Transcription Response to Mitochondrial Respiratory Stress
Article Snippet: The cRel , C/EBP δ, NFAT , and CREB genes were cloned into the pCMV4 expression vector. hnRNP A2 cDNA was also subcloned from pET28a (+) vector into pCI for transfections of C2C12 cells. .. The gal4 fusion constructs were generated by cloning the full-length (1-342 aa) and the deletion constructs (1-180 aa, 90-242 aa, 178-342 aa, and 240-342 aa) in frame into the EcoRV and HindIII sites of the pBIND gal4 dbd (Checkmate Mammalian 2-hybrid system, Promega). .. Murine C2C12 skeletal myoblasts (CRL1772; American Type Culture Collection, Manassas, VA) were grown in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum and 0.1% gentamicin. mtDNA-depleted clones containing ∼80% reduced mtDNA contents were generated as described previously ( ) and grown in the presence of 1 mM sodium pyruvate and 50 μg/ml uridine.

Clone Assay:

Article Title: Heterogeneous Nuclear Ribonucleoprotein A2 Is a Common Transcriptional Coactivator in the Nuclear Transcription Response to Mitochondrial Respiratory Stress
Article Snippet: The cRel , C/EBP δ, NFAT , and CREB genes were cloned into the pCMV4 expression vector. hnRNP A2 cDNA was also subcloned from pET28a (+) vector into pCI for transfections of C2C12 cells. .. The gal4 fusion constructs were generated by cloning the full-length (1-342 aa) and the deletion constructs (1-180 aa, 90-242 aa, 178-342 aa, and 240-342 aa) in frame into the EcoRV and HindIII sites of the pBIND gal4 dbd (Checkmate Mammalian 2-hybrid system, Promega). .. Murine C2C12 skeletal myoblasts (CRL1772; American Type Culture Collection, Manassas, VA) were grown in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum and 0.1% gentamicin. mtDNA-depleted clones containing ∼80% reduced mtDNA contents were generated as described previously ( ) and grown in the presence of 1 mM sodium pyruvate and 50 μg/ml uridine.

Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
Article Snippet: Sequences were prepared with DYEnamic ET dye terminator cycle sequencing kit for MegaBACE DNA Analysis Systems (GE Healthcare, Chalfont St. Giles, United Kingdom) and run on a MegaBACE 1000 (GE Healthcare) in the Genome Sequencing Service Laboratory at the University of Florida. .. To prepare for mutagenesis, a 1.7 kb gag-pol fragment was digested from the pLAI.4-posttherapy gag-pol recombinant virus using the restriction enzymes SphI (NEB) and EcoRV (NEB) and ligated into the pGEM5Zf+ cloning vector (Promega, Madison, WI) that was previously digested with SphI and EcoRV and treated with calf intestinal alkaline phosphatase (CIAP, Promega). .. Following transformation of DH5α cells (Invitrogen, Carlsbad, CA), ten clones were picked, grown overnight at 37°C, and plasmid DNA was extracted using a QIAprep™ Miniprep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol.

Isolation:

Article Title: Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission
Article Snippet: Transgene insertions in individual G1 and G2 chickens were analyzed first by PCR and subsequently by Southern blot analysis. .. Genomic DNA (10 μg) was isolated from whole blood, digested with EcoRV (Promega), resolved on a 1.0% (w/v) agarose gel, and then transferred to a nylon membrane (Roche). ..

Agarose Gel Electrophoresis:

Article Title: Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission
Article Snippet: Transgene insertions in individual G1 and G2 chickens were analyzed first by PCR and subsequently by Southern blot analysis. .. Genomic DNA (10 μg) was isolated from whole blood, digested with EcoRV (Promega), resolved on a 1.0% (w/v) agarose gel, and then transferred to a nylon membrane (Roche). ..

Article Title: Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality
Article Snippet: DNA from the spleen was prepared with DNeasy kits (Qiagen, Valencia, CA). .. DNA was digested with EcoR V (Promega), fractionated by agarose gel electrophoresis, and transferred to a nylon membrane (Bio-Rad). ..

Polymerase Chain Reaction:

Article Title: Sialylation of Group B Streptococcal Capsular Polysaccharide Is Mediated by cpsK and Is Required for Optimal Capsule Polymerization and Expression
Article Snippet: The insert DNA containing the cat replacement and flanking cps sequences was amplified from pDC147 by PCR using primers cps 44 and cps 71a and high-fidelity Taq polymerase. .. The cat containing PCR fragment from pDC147 was ligated to a pHY304 T-vector created by EcoRV digestion, followed by addition of deoxyribosylthymine 3′ overhangs using terminal deoxynucleotidyl transferase and dTTP (Promega). .. The ligation reaction mixture was introduced into DH5α by electroporation.

Purification:

Article Title: A dehydrin gene isolated from feral olive enhances drought tolerance in Arabidopsis transgenic plants
Article Snippet: .. Briefly, after extraction and purification, gDNA (10 μg) was digested overnight at 37°C with EcoRV and XbaI endonucleases (Promega, Italy), which do not cut in the probe. .. The digested DNA fragments were separated on agarose gel 0.8% (w/v) and blotted onto a nylon membrane Hybond-N+ (Amersham Pharmacia Biotech, Milan, Italy), using the Vacuum Blotting System (BioRad, Milan, Italy).

Article Title: Aromaticity at the water-hydrocarbon core interface of the membrane
Article Snippet: The resultant digestion was transformed in TOP10 Escherichia coli ). .. The cDNA/plasmid for each subunit was linearized by restriction enzyme EcoRV for α, δ and ε and Hind III for β, and purified by Wizard DNA CleanUp System (Promega, Madison, WI, USA). .. One microgram of linearized cDNA/plasmid was used as template for in vitro-transcription reaction by mMessenger mMachine kit (Ambion, Austin, TX, USA).

Plasmid Preparation:

Article Title: Functional evaluation of a homologue of plant rapid alkalinisation factor (RALF) peptides in Fusarium graminearum
Article Snippet: The resulting amplicon was gel purified using Qiagen gel extraction kit QIAstock. .. The fragments were inserted into the EcoRV restriction site of pGEM®-T Easy Vector (Promega) using Gibson assembly Master Mix kit (New England BioLabs Inc.) according to the manufacturer’s protocol to generate vectors pAMFg5.1 and pAMFg5.2. ..

Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
Article Snippet: Sequences were prepared with DYEnamic ET dye terminator cycle sequencing kit for MegaBACE DNA Analysis Systems (GE Healthcare, Chalfont St. Giles, United Kingdom) and run on a MegaBACE 1000 (GE Healthcare) in the Genome Sequencing Service Laboratory at the University of Florida. .. To prepare for mutagenesis, a 1.7 kb gag-pol fragment was digested from the pLAI.4-posttherapy gag-pol recombinant virus using the restriction enzymes SphI (NEB) and EcoRV (NEB) and ligated into the pGEM5Zf+ cloning vector (Promega, Madison, WI) that was previously digested with SphI and EcoRV and treated with calf intestinal alkaline phosphatase (CIAP, Promega). .. Following transformation of DH5α cells (Invitrogen, Carlsbad, CA), ten clones were picked, grown overnight at 37°C, and plasmid DNA was extracted using a QIAprep™ Miniprep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol.

Mutagenesis:

Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
Article Snippet: Sequences were prepared with DYEnamic ET dye terminator cycle sequencing kit for MegaBACE DNA Analysis Systems (GE Healthcare, Chalfont St. Giles, United Kingdom) and run on a MegaBACE 1000 (GE Healthcare) in the Genome Sequencing Service Laboratory at the University of Florida. .. To prepare for mutagenesis, a 1.7 kb gag-pol fragment was digested from the pLAI.4-posttherapy gag-pol recombinant virus using the restriction enzymes SphI (NEB) and EcoRV (NEB) and ligated into the pGEM5Zf+ cloning vector (Promega, Madison, WI) that was previously digested with SphI and EcoRV and treated with calf intestinal alkaline phosphatase (CIAP, Promega). .. Following transformation of DH5α cells (Invitrogen, Carlsbad, CA), ten clones were picked, grown overnight at 37°C, and plasmid DNA was extracted using a QIAprep™ Miniprep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol.

Recombinant:

Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
Article Snippet: Sequences were prepared with DYEnamic ET dye terminator cycle sequencing kit for MegaBACE DNA Analysis Systems (GE Healthcare, Chalfont St. Giles, United Kingdom) and run on a MegaBACE 1000 (GE Healthcare) in the Genome Sequencing Service Laboratory at the University of Florida. .. To prepare for mutagenesis, a 1.7 kb gag-pol fragment was digested from the pLAI.4-posttherapy gag-pol recombinant virus using the restriction enzymes SphI (NEB) and EcoRV (NEB) and ligated into the pGEM5Zf+ cloning vector (Promega, Madison, WI) that was previously digested with SphI and EcoRV and treated with calf intestinal alkaline phosphatase (CIAP, Promega). .. Following transformation of DH5α cells (Invitrogen, Carlsbad, CA), ten clones were picked, grown overnight at 37°C, and plasmid DNA was extracted using a QIAprep™ Miniprep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol.

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    Promega ecorv
    Southern blot analysis of the transgenic chickens. Genomic <t>DNA</t> samples were extracted from whole blood samples from wild-type and transgenic chickens (G1 and G2), digested with <t>EcoRV,</t> electrophoresed, blotted, and hybridized with a probe for the 3D8 scFv gene. M, DIG-labeled DNA marker; lane 1, wild-type chicken; lane 2, P-54; lane 3, P-54 offspring; lane 4, N-82; lane 5, N-95; lane 6, N-165; lane 7, N-183; lane 8, P-196; lane 9, N-238.
    Ecorv, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv/product/Promega
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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    Southern blot analysis of the transgenic chickens. Genomic DNA samples were extracted from whole blood samples from wild-type and transgenic chickens (G1 and G2), digested with EcoRV, electrophoresed, blotted, and hybridized with a probe for the 3D8 scFv gene. M, DIG-labeled DNA marker; lane 1, wild-type chicken; lane 2, P-54; lane 3, P-54 offspring; lane 4, N-82; lane 5, N-95; lane 6, N-165; lane 7, N-183; lane 8, P-196; lane 9, N-238.

    Journal: Scientific Reports

    Article Title: Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission

    doi: 10.1038/s41598-017-05270-8

    Figure Lengend Snippet: Southern blot analysis of the transgenic chickens. Genomic DNA samples were extracted from whole blood samples from wild-type and transgenic chickens (G1 and G2), digested with EcoRV, electrophoresed, blotted, and hybridized with a probe for the 3D8 scFv gene. M, DIG-labeled DNA marker; lane 1, wild-type chicken; lane 2, P-54; lane 3, P-54 offspring; lane 4, N-82; lane 5, N-95; lane 6, N-165; lane 7, N-183; lane 8, P-196; lane 9, N-238.

    Article Snippet: Genomic DNA (10 μg) was isolated from whole blood, digested with EcoRV (Promega), resolved on a 1.0% (w/v) agarose gel, and then transferred to a nylon membrane (Roche).

    Techniques: Southern Blot, Transgenic Assay, Labeling, Marker