Structured Review

GenScript ecorv
Ecorv, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecorv/product/GenScript
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
ecorv - by Bioz Stars, 2020-07
92/100 stars

Images

Related Articles

Clone Assay:

Article Title: Networks of intergenic long-range enhancers and snpRNAs drive castration-resistant phenotype of prostate cancer and contribute to pathogenesis of multiple common human disorders
Article Snippet: .. Allele-specific sense and antisense variants of the rs2670660 sequence of 52 nt in length ( in ref. ; nucleotide sequence shown in shaded box) were chemically synthesized and cloned sequentially into pUC57 plasmid by EcoRV (GeneScript Corporation) and pCDH-CMV-MCS-EF1-copGFP plasmid by EcoRI and NotI (SystemsBio). .. The integrity and molecular identity of the synthetic sequences as well as designed plasmid vectors were monitored by restriction enzyme-mapping analysis and direct sequencing.

Article Title: Use of the Meganuclease I-SceI of Saccharomycescerevisiae to select for gene deletions in actinomycetes
Article Snippet: .. A fragment containing the tcp830 promoter , followed by the synthetic I-SceI gene codon optimised for actinomycetes, the Streptomyces lividans multi-copy plasmid pIJ101 terminator region , a multiple cloning site (MCS) containing the following unique sites: SacII, NotI, XbaI, SpeI, PstI, EcoRI and EcoRV and the I-SceI 18 bp recognition sequence was synthesised by GeneScript (Piscataway, USA) and flanked by HindIII and MfeI restriction sites. .. The fragment was provided on the plasmid pUC57-Simple_SceI.

Article Title: Coiled Coils Ensure the Physiological Ectodomain Shedding of Collagen XVII *
Article Snippet: .. For site-directed mutagenesis of the coiled-coil sequence, two different 1187-bp fragments spanning the EcoRV and ClaI DNA restriction sites, which contain substitution mutations of either Leu495 or both Leu502 and Leu495 into Pro, were chemically produced and cloned into pUC57 (GenScript) ( B ). .. EcoRV- and ClaI-digested DNA fragments were then inserted into the COL17-pcDNA5/FRT vector.

Article Title: Nanotopography enhances dynamic remodeling of tight junction proteins through cytosolic complexes
Article Snippet: .. Sequence of repair plasmid for HDR-based mCherry knock-in The following 2747bp gene was cloned in pUC57-Kan plasmid by EcoRV (Genscript, Inc). ..

Article Title: Rapid, field-deployable nucleobase detection and identification using FnCas9
Article Snippet: .. Around region of around 500bp including SNV/mismatches for four Mendelian disorders Glanzman, Thrombasthenia, Hemophilia A (Factor VIII deficiency), Glycogen Storage Disease Type I and X-linked myotubular myopathy were procured as synthetic DNA oligos and cloned into the pUC57 by EcoRV (GENESCRIPT). .. Similarly, a 500bp region flanking two SNVs (A2142G and A2143G) in Helicobacter pylori 23s rRNA gene were ordered as synthetic DNA cloned in pUC57 by EcoRV.

Positron Emission Tomography:

Article Title: Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5?-terminal regions of cap0-, cap1- and 5?ppp- mRNAs
Article Snippet: .. The transcription vectors for β-globin, ATF4, GCN4 and Sncb mRNAs were made by inserting DNA sequences (corresponding to their 5′-terminal 235, 442, 600 and 247 nt, respectively) flanked by a T7 promoter and HindIII, EcoRV, EcoRV and HindIII restriction sites, respectively, into pUC57 (GenScript). pET-28a(rIFIT1) for expression of rIFIT1 (NCBI Reference sequence XP_002718421.1) and pET16b(rIFT1B) for expression of rIFIT1B (NCBI Reference sequence XP_002718420.1) were made by Biomatik Inc. (Cambridge, ON, Canada) by inserting synthetic DNA sequences between Nhe1 and BamH1 sites of pET28a(+) and Nde1 and BamH1 sites of pET16b (Novagen), respectively. pET16b(hIFIT1B) for expression of hIFIT1B was made by inserting a DNA fragment amplified by polymerase chain reaction from plasmid HsCD00342660/MGC: 168989 (Genbank Acc. .. No. BC137368) from the DF/HCC DNA Resource Core (Harvard Medical School) between Nde1 and BamH1 sites of pET16b. hIFIT1 mutants were generated by NorClone Biotech Laboratories (London, ON, Canada) using a wt IFIT1 expression vector ( ). mRNAs and tRNAs were in vitro transcribed using T7 polymerase.

Synthesized:

Article Title: Networks of intergenic long-range enhancers and snpRNAs drive castration-resistant phenotype of prostate cancer and contribute to pathogenesis of multiple common human disorders
Article Snippet: .. Allele-specific sense and antisense variants of the rs2670660 sequence of 52 nt in length ( in ref. ; nucleotide sequence shown in shaded box) were chemically synthesized and cloned sequentially into pUC57 plasmid by EcoRV (GeneScript Corporation) and pCDH-CMV-MCS-EF1-copGFP plasmid by EcoRI and NotI (SystemsBio). .. The integrity and molecular identity of the synthetic sequences as well as designed plasmid vectors were monitored by restriction enzyme-mapping analysis and direct sequencing.

Mutagenesis:

Article Title: Coiled Coils Ensure the Physiological Ectodomain Shedding of Collagen XVII *
Article Snippet: .. For site-directed mutagenesis of the coiled-coil sequence, two different 1187-bp fragments spanning the EcoRV and ClaI DNA restriction sites, which contain substitution mutations of either Leu495 or both Leu502 and Leu495 into Pro, were chemically produced and cloned into pUC57 (GenScript) ( B ). .. EcoRV- and ClaI-digested DNA fragments were then inserted into the COL17-pcDNA5/FRT vector.

Sequencing:

Article Title: Gene editing in clinical isolates of Candida parapsilosis using CRISPR/Cas9
Article Snippet: .. The 5′ ends of gBlock-1 and -4 and the 3′ ends of gBlocks-3 and -6 include 25–50 bp that overlap with the sequence surrounding EcoRV in plasmid pUC57 (GenScript). .. Each gBlock includes 25–50 bp overlap with the adjacent gBlock. gBlocks were amplified by PCR using primers A-L (Supplementary Table ). gBlocks-1/2/3 and gBlocks-4/5/6 were independently cloned into EcoRV-digested pUC57 by Gibson assembly (NEB) , generating plasmids pUC57-CAS9fr1 and pUC57-CAS9fr2.

Article Title: Networks of intergenic long-range enhancers and snpRNAs drive castration-resistant phenotype of prostate cancer and contribute to pathogenesis of multiple common human disorders
Article Snippet: .. Allele-specific sense and antisense variants of the rs2670660 sequence of 52 nt in length ( in ref. ; nucleotide sequence shown in shaded box) were chemically synthesized and cloned sequentially into pUC57 plasmid by EcoRV (GeneScript Corporation) and pCDH-CMV-MCS-EF1-copGFP plasmid by EcoRI and NotI (SystemsBio). .. The integrity and molecular identity of the synthetic sequences as well as designed plasmid vectors were monitored by restriction enzyme-mapping analysis and direct sequencing.

Article Title: Use of the Meganuclease I-SceI of Saccharomycescerevisiae to select for gene deletions in actinomycetes
Article Snippet: .. A fragment containing the tcp830 promoter , followed by the synthetic I-SceI gene codon optimised for actinomycetes, the Streptomyces lividans multi-copy plasmid pIJ101 terminator region , a multiple cloning site (MCS) containing the following unique sites: SacII, NotI, XbaI, SpeI, PstI, EcoRI and EcoRV and the I-SceI 18 bp recognition sequence was synthesised by GeneScript (Piscataway, USA) and flanked by HindIII and MfeI restriction sites. .. The fragment was provided on the plasmid pUC57-Simple_SceI.

Article Title: Coiled Coils Ensure the Physiological Ectodomain Shedding of Collagen XVII *
Article Snippet: .. For site-directed mutagenesis of the coiled-coil sequence, two different 1187-bp fragments spanning the EcoRV and ClaI DNA restriction sites, which contain substitution mutations of either Leu495 or both Leu502 and Leu495 into Pro, were chemically produced and cloned into pUC57 (GenScript) ( B ). .. EcoRV- and ClaI-digested DNA fragments were then inserted into the COL17-pcDNA5/FRT vector.

Article Title: Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5?-terminal regions of cap0-, cap1- and 5?ppp- mRNAs
Article Snippet: .. The transcription vectors for β-globin, ATF4, GCN4 and Sncb mRNAs were made by inserting DNA sequences (corresponding to their 5′-terminal 235, 442, 600 and 247 nt, respectively) flanked by a T7 promoter and HindIII, EcoRV, EcoRV and HindIII restriction sites, respectively, into pUC57 (GenScript). pET-28a(rIFIT1) for expression of rIFIT1 (NCBI Reference sequence XP_002718421.1) and pET16b(rIFT1B) for expression of rIFIT1B (NCBI Reference sequence XP_002718420.1) were made by Biomatik Inc. (Cambridge, ON, Canada) by inserting synthetic DNA sequences between Nhe1 and BamH1 sites of pET28a(+) and Nde1 and BamH1 sites of pET16b (Novagen), respectively. pET16b(hIFIT1B) for expression of hIFIT1B was made by inserting a DNA fragment amplified by polymerase chain reaction from plasmid HsCD00342660/MGC: 168989 (Genbank Acc. .. No. BC137368) from the DF/HCC DNA Resource Core (Harvard Medical School) between Nde1 and BamH1 sites of pET16b. hIFIT1 mutants were generated by NorClone Biotech Laboratories (London, ON, Canada) using a wt IFIT1 expression vector ( ). mRNAs and tRNAs were in vitro transcribed using T7 polymerase.

Article Title: Nanotopography enhances dynamic remodeling of tight junction proteins through cytosolic complexes
Article Snippet: .. Sequence of repair plasmid for HDR-based mCherry knock-in The following 2747bp gene was cloned in pUC57-Kan plasmid by EcoRV (Genscript, Inc). ..

Produced:

Article Title: Coiled Coils Ensure the Physiological Ectodomain Shedding of Collagen XVII *
Article Snippet: .. For site-directed mutagenesis of the coiled-coil sequence, two different 1187-bp fragments spanning the EcoRV and ClaI DNA restriction sites, which contain substitution mutations of either Leu495 or both Leu502 and Leu495 into Pro, were chemically produced and cloned into pUC57 (GenScript) ( B ). .. EcoRV- and ClaI-digested DNA fragments were then inserted into the COL17-pcDNA5/FRT vector.

Polymerase Chain Reaction:

Article Title: Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5?-terminal regions of cap0-, cap1- and 5?ppp- mRNAs
Article Snippet: .. The transcription vectors for β-globin, ATF4, GCN4 and Sncb mRNAs were made by inserting DNA sequences (corresponding to their 5′-terminal 235, 442, 600 and 247 nt, respectively) flanked by a T7 promoter and HindIII, EcoRV, EcoRV and HindIII restriction sites, respectively, into pUC57 (GenScript). pET-28a(rIFIT1) for expression of rIFIT1 (NCBI Reference sequence XP_002718421.1) and pET16b(rIFT1B) for expression of rIFIT1B (NCBI Reference sequence XP_002718420.1) were made by Biomatik Inc. (Cambridge, ON, Canada) by inserting synthetic DNA sequences between Nhe1 and BamH1 sites of pET28a(+) and Nde1 and BamH1 sites of pET16b (Novagen), respectively. pET16b(hIFIT1B) for expression of hIFIT1B was made by inserting a DNA fragment amplified by polymerase chain reaction from plasmid HsCD00342660/MGC: 168989 (Genbank Acc. .. No. BC137368) from the DF/HCC DNA Resource Core (Harvard Medical School) between Nde1 and BamH1 sites of pET16b. hIFIT1 mutants were generated by NorClone Biotech Laboratories (London, ON, Canada) using a wt IFIT1 expression vector ( ). mRNAs and tRNAs were in vitro transcribed using T7 polymerase.

Amplification:

Article Title: Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5?-terminal regions of cap0-, cap1- and 5?ppp- mRNAs
Article Snippet: .. The transcription vectors for β-globin, ATF4, GCN4 and Sncb mRNAs were made by inserting DNA sequences (corresponding to their 5′-terminal 235, 442, 600 and 247 nt, respectively) flanked by a T7 promoter and HindIII, EcoRV, EcoRV and HindIII restriction sites, respectively, into pUC57 (GenScript). pET-28a(rIFIT1) for expression of rIFIT1 (NCBI Reference sequence XP_002718421.1) and pET16b(rIFT1B) for expression of rIFIT1B (NCBI Reference sequence XP_002718420.1) were made by Biomatik Inc. (Cambridge, ON, Canada) by inserting synthetic DNA sequences between Nhe1 and BamH1 sites of pET28a(+) and Nde1 and BamH1 sites of pET16b (Novagen), respectively. pET16b(hIFIT1B) for expression of hIFIT1B was made by inserting a DNA fragment amplified by polymerase chain reaction from plasmid HsCD00342660/MGC: 168989 (Genbank Acc. .. No. BC137368) from the DF/HCC DNA Resource Core (Harvard Medical School) between Nde1 and BamH1 sites of pET16b. hIFIT1 mutants were generated by NorClone Biotech Laboratories (London, ON, Canada) using a wt IFIT1 expression vector ( ). mRNAs and tRNAs were in vitro transcribed using T7 polymerase.

Expressing:

Article Title: Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5?-terminal regions of cap0-, cap1- and 5?ppp- mRNAs
Article Snippet: .. The transcription vectors for β-globin, ATF4, GCN4 and Sncb mRNAs were made by inserting DNA sequences (corresponding to their 5′-terminal 235, 442, 600 and 247 nt, respectively) flanked by a T7 promoter and HindIII, EcoRV, EcoRV and HindIII restriction sites, respectively, into pUC57 (GenScript). pET-28a(rIFIT1) for expression of rIFIT1 (NCBI Reference sequence XP_002718421.1) and pET16b(rIFT1B) for expression of rIFIT1B (NCBI Reference sequence XP_002718420.1) were made by Biomatik Inc. (Cambridge, ON, Canada) by inserting synthetic DNA sequences between Nhe1 and BamH1 sites of pET28a(+) and Nde1 and BamH1 sites of pET16b (Novagen), respectively. pET16b(hIFIT1B) for expression of hIFIT1B was made by inserting a DNA fragment amplified by polymerase chain reaction from plasmid HsCD00342660/MGC: 168989 (Genbank Acc. .. No. BC137368) from the DF/HCC DNA Resource Core (Harvard Medical School) between Nde1 and BamH1 sites of pET16b. hIFIT1 mutants were generated by NorClone Biotech Laboratories (London, ON, Canada) using a wt IFIT1 expression vector ( ). mRNAs and tRNAs were in vitro transcribed using T7 polymerase.

Knock-In:

Article Title: Nanotopography enhances dynamic remodeling of tight junction proteins through cytosolic complexes
Article Snippet: .. Sequence of repair plasmid for HDR-based mCherry knock-in The following 2747bp gene was cloned in pUC57-Kan plasmid by EcoRV (Genscript, Inc). ..

Plasmid Preparation:

Article Title: Gene editing in clinical isolates of Candida parapsilosis using CRISPR/Cas9
Article Snippet: .. The 5′ ends of gBlock-1 and -4 and the 3′ ends of gBlocks-3 and -6 include 25–50 bp that overlap with the sequence surrounding EcoRV in plasmid pUC57 (GenScript). .. Each gBlock includes 25–50 bp overlap with the adjacent gBlock. gBlocks were amplified by PCR using primers A-L (Supplementary Table ). gBlocks-1/2/3 and gBlocks-4/5/6 were independently cloned into EcoRV-digested pUC57 by Gibson assembly (NEB) , generating plasmids pUC57-CAS9fr1 and pUC57-CAS9fr2.

Article Title: Networks of intergenic long-range enhancers and snpRNAs drive castration-resistant phenotype of prostate cancer and contribute to pathogenesis of multiple common human disorders
Article Snippet: .. Allele-specific sense and antisense variants of the rs2670660 sequence of 52 nt in length ( in ref. ; nucleotide sequence shown in shaded box) were chemically synthesized and cloned sequentially into pUC57 plasmid by EcoRV (GeneScript Corporation) and pCDH-CMV-MCS-EF1-copGFP plasmid by EcoRI and NotI (SystemsBio). .. The integrity and molecular identity of the synthetic sequences as well as designed plasmid vectors were monitored by restriction enzyme-mapping analysis and direct sequencing.

Article Title: Use of the Meganuclease I-SceI of Saccharomycescerevisiae to select for gene deletions in actinomycetes
Article Snippet: .. A fragment containing the tcp830 promoter , followed by the synthetic I-SceI gene codon optimised for actinomycetes, the Streptomyces lividans multi-copy plasmid pIJ101 terminator region , a multiple cloning site (MCS) containing the following unique sites: SacII, NotI, XbaI, SpeI, PstI, EcoRI and EcoRV and the I-SceI 18 bp recognition sequence was synthesised by GeneScript (Piscataway, USA) and flanked by HindIII and MfeI restriction sites. .. The fragment was provided on the plasmid pUC57-Simple_SceI.

Article Title: Inhibition of translation by IFIT family members is determined by their ability to interact selectively with the 5?-terminal regions of cap0-, cap1- and 5?ppp- mRNAs
Article Snippet: .. The transcription vectors for β-globin, ATF4, GCN4 and Sncb mRNAs were made by inserting DNA sequences (corresponding to their 5′-terminal 235, 442, 600 and 247 nt, respectively) flanked by a T7 promoter and HindIII, EcoRV, EcoRV and HindIII restriction sites, respectively, into pUC57 (GenScript). pET-28a(rIFIT1) for expression of rIFIT1 (NCBI Reference sequence XP_002718421.1) and pET16b(rIFT1B) for expression of rIFIT1B (NCBI Reference sequence XP_002718420.1) were made by Biomatik Inc. (Cambridge, ON, Canada) by inserting synthetic DNA sequences between Nhe1 and BamH1 sites of pET28a(+) and Nde1 and BamH1 sites of pET16b (Novagen), respectively. pET16b(hIFIT1B) for expression of hIFIT1B was made by inserting a DNA fragment amplified by polymerase chain reaction from plasmid HsCD00342660/MGC: 168989 (Genbank Acc. .. No. BC137368) from the DF/HCC DNA Resource Core (Harvard Medical School) between Nde1 and BamH1 sites of pET16b. hIFIT1 mutants were generated by NorClone Biotech Laboratories (London, ON, Canada) using a wt IFIT1 expression vector ( ). mRNAs and tRNAs were in vitro transcribed using T7 polymerase.

Article Title: Nanotopography enhances dynamic remodeling of tight junction proteins through cytosolic complexes
Article Snippet: .. Sequence of repair plasmid for HDR-based mCherry knock-in The following 2747bp gene was cloned in pUC57-Kan plasmid by EcoRV (Genscript, Inc). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    GenScript ecorv site
    Ecorv Site, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv site/product/GenScript
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    ecorv site - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    GenScript nhe1 ecorv restriction sites
    Nhe1 Ecorv Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhe1 ecorv restriction sites/product/GenScript
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhe1 ecorv restriction sites - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    GenScript puc57 ecorv cspba myc3 spei plasmid
    Puc57 Ecorv Cspba Myc3 Spei Plasmid, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc57 ecorv cspba myc3 spei plasmid/product/GenScript
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    puc57 ecorv cspba myc3 spei plasmid - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results