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GenScript ecorv site
Ecorv Site, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecorv site/product/GenScript
Average 93 stars, based on 24 article reviews
Price from $9.99 to $1999.99
ecorv site - by Bioz Stars, 2020-08
93/100 stars

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Clone Assay:

Article Title: Genetically Programmable Thermoresponsive Plasmonic Gold/Silk-Elastin Protein Core/Shell Nanoparticles
Article Snippet: .. The monomer DNA sequence was purchased as synthetic gene that was cloned into EcoRV site of the vector pUC57 from GenScript. .. The BanII restriction sites were designed to flank the monomer DNA sequence.

Article Title: Expression of EZH2 is associated with poor outcome in colorectal cancer
Article Snippet: .. The donor sequences were presented in and cloned into the EcoRV site of pUC57 (GenScript). .. To generate EZH2 tyrosine 641 mutant cell lines, two gRNA constructs along with the donor sequence construct were co-transfected into the HCEC cell line using Lipofectamine (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol ( ).

Article Title: The Spleen Tyrosine Kinase (Syk) Regulates Alzheimer Amyloid-β Production and Tau Hyperphosphorylation *
Article Snippet: .. The synthesized gene (SykA6H) was blunt-end cloned into pUC57 using an EcoRV site to give pSykAvi6H; the gene sequence was verified by DNA sequencing (GenScript). .. The pSykAvi6H plasmid was digested with BamHI and EcoRI, and the SykAvi6H gene was ligated into pBac-1 that had been digested with the same restriction enzymes to give pBacSykAvi6H.

Article Title: CRISPR Gene Editing in Yeast: An Experimental Protocol for an Upper-Division Undergraduate Laboratory Course
Article Snippet: .. The gap repair DNA fragments for ADE2 and STE12 ( ) were commercially synthesized and cloned into the EcoRV site of pUC57 by Genscript. .. These plasmids were also transformed in to DH5α cells, followed by plasmid mini prep from one single colony.

Article Title: Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family
Article Snippet: .. Three putative AcXE-encoding genes were codon optimized, synthesized, and cloned into the EcoRV site of a pUC57 vector (2,710 bp; GenScript, Piscataway, NJ, USA). .. Putative AcXE-encoding genes were PCR amplified using specific primers ( ) and Dream Taq DNA polymerase (ThermoScientific, MA, USA).

Article Title: A Transgenic Mouse Model to Selectively Identify α3 Na,K-ATPase Expressing Cells in the Nervous System
Article Snippet: .. A portion of the mouse Atp1a3 gene (promoter and entire 5’-untranslated region) linked to the expression cassette from pZsGreen1–1 (Clontech, Palo Alto, CA, USA) was synthesized and cloned into the EcoRV site of pUC57 by GenScript (Piscataway, NJ, USA) to generate the Atp1a3-ZsGreen1 plasmid. .. The Atp1a3 sequence spans positions −1578 to +177 (relative to the transcription start site), and is highly conserved between human, rat and mouse.

Synthesized:

Article Title: Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase
Article Snippet: .. The DNA sequence encoding a 10x His tag followed by maltose-binding protein (MBP) and a HRV 3C site was codon optimized for expression in insect cells, synthesized, and sub-cloned into the EcoRV site of the pUC57 vector by GenScript (Piscataway, NJ), yielding the vector pUC57-10His-MBP-3C. .. The 10His-MBP-3C sequence was then PCR amplified using pUC57-10His-MBP-3C as the template, and the PCR product was cloned immediately 5΄ of the BamHI site of the pKL vector via the SLIC method , yielding the pKL-10His-MBP-3C vector.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Article Title: The Spleen Tyrosine Kinase (Syk) Regulates Alzheimer Amyloid-β Production and Tau Hyperphosphorylation *
Article Snippet: .. The synthesized gene (SykA6H) was blunt-end cloned into pUC57 using an EcoRV site to give pSykAvi6H; the gene sequence was verified by DNA sequencing (GenScript). .. The pSykAvi6H plasmid was digested with BamHI and EcoRI, and the SykAvi6H gene was ligated into pBac-1 that had been digested with the same restriction enzymes to give pBacSykAvi6H.

Article Title: CRISPR Gene Editing in Yeast: An Experimental Protocol for an Upper-Division Undergraduate Laboratory Course
Article Snippet: .. The gap repair DNA fragments for ADE2 and STE12 ( ) were commercially synthesized and cloned into the EcoRV site of pUC57 by Genscript. .. These plasmids were also transformed in to DH5α cells, followed by plasmid mini prep from one single colony.

Article Title: Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family
Article Snippet: .. Three putative AcXE-encoding genes were codon optimized, synthesized, and cloned into the EcoRV site of a pUC57 vector (2,710 bp; GenScript, Piscataway, NJ, USA). .. Putative AcXE-encoding genes were PCR amplified using specific primers ( ) and Dream Taq DNA polymerase (ThermoScientific, MA, USA).

Article Title: A Transgenic Mouse Model to Selectively Identify α3 Na,K-ATPase Expressing Cells in the Nervous System
Article Snippet: .. A portion of the mouse Atp1a3 gene (promoter and entire 5’-untranslated region) linked to the expression cassette from pZsGreen1–1 (Clontech, Palo Alto, CA, USA) was synthesized and cloned into the EcoRV site of pUC57 by GenScript (Piscataway, NJ, USA) to generate the Atp1a3-ZsGreen1 plasmid. .. The Atp1a3 sequence spans positions −1578 to +177 (relative to the transcription start site), and is highly conserved between human, rat and mouse.

DNA Sequencing:

Article Title: The Spleen Tyrosine Kinase (Syk) Regulates Alzheimer Amyloid-β Production and Tau Hyperphosphorylation *
Article Snippet: .. The synthesized gene (SykA6H) was blunt-end cloned into pUC57 using an EcoRV site to give pSykAvi6H; the gene sequence was verified by DNA sequencing (GenScript). .. The pSykAvi6H plasmid was digested with BamHI and EcoRI, and the SykAvi6H gene was ligated into pBac-1 that had been digested with the same restriction enzymes to give pBacSykAvi6H.

Expressing:

Article Title: Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase
Article Snippet: .. The DNA sequence encoding a 10x His tag followed by maltose-binding protein (MBP) and a HRV 3C site was codon optimized for expression in insect cells, synthesized, and sub-cloned into the EcoRV site of the pUC57 vector by GenScript (Piscataway, NJ), yielding the vector pUC57-10His-MBP-3C. .. The 10His-MBP-3C sequence was then PCR amplified using pUC57-10His-MBP-3C as the template, and the PCR product was cloned immediately 5΄ of the BamHI site of the pKL vector via the SLIC method , yielding the pKL-10His-MBP-3C vector.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Article Title: A Transgenic Mouse Model to Selectively Identify α3 Na,K-ATPase Expressing Cells in the Nervous System
Article Snippet: .. A portion of the mouse Atp1a3 gene (promoter and entire 5’-untranslated region) linked to the expression cassette from pZsGreen1–1 (Clontech, Palo Alto, CA, USA) was synthesized and cloned into the EcoRV site of pUC57 by GenScript (Piscataway, NJ, USA) to generate the Atp1a3-ZsGreen1 plasmid. .. The Atp1a3 sequence spans positions −1578 to +177 (relative to the transcription start site), and is highly conserved between human, rat and mouse.

Sequencing:

Article Title: Genetically Programmable Thermoresponsive Plasmonic Gold/Silk-Elastin Protein Core/Shell Nanoparticles
Article Snippet: .. The monomer DNA sequence was purchased as synthetic gene that was cloned into EcoRV site of the vector pUC57 from GenScript. .. The BanII restriction sites were designed to flank the monomer DNA sequence.

Article Title: Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase
Article Snippet: .. The DNA sequence encoding a 10x His tag followed by maltose-binding protein (MBP) and a HRV 3C site was codon optimized for expression in insect cells, synthesized, and sub-cloned into the EcoRV site of the pUC57 vector by GenScript (Piscataway, NJ), yielding the vector pUC57-10His-MBP-3C. .. The 10His-MBP-3C sequence was then PCR amplified using pUC57-10His-MBP-3C as the template, and the PCR product was cloned immediately 5΄ of the BamHI site of the pKL vector via the SLIC method , yielding the pKL-10His-MBP-3C vector.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Article Title: The Spleen Tyrosine Kinase (Syk) Regulates Alzheimer Amyloid-β Production and Tau Hyperphosphorylation *
Article Snippet: .. The synthesized gene (SykA6H) was blunt-end cloned into pUC57 using an EcoRV site to give pSykAvi6H; the gene sequence was verified by DNA sequencing (GenScript). .. The pSykAvi6H plasmid was digested with BamHI and EcoRI, and the SykAvi6H gene was ligated into pBac-1 that had been digested with the same restriction enzymes to give pBacSykAvi6H.

Plasmid Preparation:

Article Title: Genetically Programmable Thermoresponsive Plasmonic Gold/Silk-Elastin Protein Core/Shell Nanoparticles
Article Snippet: .. The monomer DNA sequence was purchased as synthetic gene that was cloned into EcoRV site of the vector pUC57 from GenScript. .. The BanII restriction sites were designed to flank the monomer DNA sequence.

Article Title: Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase
Article Snippet: .. The DNA sequence encoding a 10x His tag followed by maltose-binding protein (MBP) and a HRV 3C site was codon optimized for expression in insect cells, synthesized, and sub-cloned into the EcoRV site of the pUC57 vector by GenScript (Piscataway, NJ), yielding the vector pUC57-10His-MBP-3C. .. The 10His-MBP-3C sequence was then PCR amplified using pUC57-10His-MBP-3C as the template, and the PCR product was cloned immediately 5΄ of the BamHI site of the pKL vector via the SLIC method , yielding the pKL-10His-MBP-3C vector.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Article Title: Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family
Article Snippet: .. Three putative AcXE-encoding genes were codon optimized, synthesized, and cloned into the EcoRV site of a pUC57 vector (2,710 bp; GenScript, Piscataway, NJ, USA). .. Putative AcXE-encoding genes were PCR amplified using specific primers ( ) and Dream Taq DNA polymerase (ThermoScientific, MA, USA).

Article Title: A Transgenic Mouse Model to Selectively Identify α3 Na,K-ATPase Expressing Cells in the Nervous System
Article Snippet: .. A portion of the mouse Atp1a3 gene (promoter and entire 5’-untranslated region) linked to the expression cassette from pZsGreen1–1 (Clontech, Palo Alto, CA, USA) was synthesized and cloned into the EcoRV site of pUC57 by GenScript (Piscataway, NJ, USA) to generate the Atp1a3-ZsGreen1 plasmid. .. The Atp1a3 sequence spans positions −1578 to +177 (relative to the transcription start site), and is highly conserved between human, rat and mouse.

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  • 91
    GenScript ecori site
    Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified <t>DHBV</t> rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The <t>EcoRI-linearized</t> DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.
    Ecori Site, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori site/product/GenScript
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ecori site - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    GenScript ecorv site
    Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified <t>DHBV</t> rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The <t>EcoRI-linearized</t> DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.
    Ecorv Site, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv site/product/GenScript
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    ecorv site - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    GenScript nhe1 ecorv restriction sites
    Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified <t>DHBV</t> rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The <t>EcoRI-linearized</t> DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.
    Nhe1 Ecorv Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhe1 ecorv restriction sites/product/GenScript
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhe1 ecorv restriction sites - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified DHBV rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The EcoRI-linearized DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.

    Journal: PLoS Pathogens

    Article Title: The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation

    doi: 10.1371/journal.ppat.1006784

    Figure Lengend Snippet: Ligase inhibitor treatment blocked cccDNA formation in nuclear extract. (A) Demonstration of the in vitro cccDNA formation assay. The indicated amount of purified DHBV rcDNA were incubated with HepG2 nuclear extract as described in Materials and Methods. After the in vitro cccDNA formation reaction, total DNA were extracted and subjected to DHBV Southern blot analysis (top panel) and cccDNA-specific PCR assay (bottom panel). The EcoRI-linearized DHBV unit-length DNA was used as marker in Southern blot and as PCR positive template in cccDNA PCR assay (lane 2). 1 ng of rcDNA alone (lane 3) and nuclear extract alone (lane 8) served as PCR negative controls. (B) The in vitro DHBV cccDNA formation reaction was treated with DNA ligases inhibitor L1 (20 μM), L25 (20 μM), or L189 (50 μM), or DMSO solvent, and cccDNA was detected by PCR (lanes 3–6). DHBV cccDNA purified from Dstet5 cells was used as PCR positive control (lane 1). 1 ng of rcDNA without incubation with nuclear extract (lane 7) or without PCR reaction (lane 8) served as negative controls.

    Article Snippet: Next, another DNA fragment containing nt 425–468 sequence from pBI, a spacer sequence (5’-GCAGAGCTCGTTTGATC-3’), and DHBV sequence (nt 2524-3021/1), with unique KpnI and EcoRI site at 5’ and 3’ end, respectively, was chemically synthesized (Genescript), and the fragment was inserted into the KpnI/EcoRI sites of pTRE-GFP-HBV to generate pTRE-GFP-DHBV-EcoRI-HBV.

    Techniques: In Vitro, Tube Formation Assay, Purification, Incubation, Southern Blot, Polymerase Chain Reaction, Marker, Positive Control