ecorv enzyme  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ecorv enzyme
    Ecorv Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv enzyme/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv enzyme - by Bioz Stars, 2021-07
    86/100 stars

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    Plasmid Preparation:

    Article Title: Pheromone Receptors Help Cnaphalocrocis medinalis Avoid Competition in Rice Fields
    Article Snippet: Gene expression levels were analyzed using the 2-ΔΔCT method, where ΔCT = CT PR gene – CT actin gene and Δ Δ CT = ΔCT different tissues – ΔCT maximum (Livak and Schmittgen 2001). .. Vector construction and cRNA synthesisThe specific primers with the Kozak consensus sequence and pT7Ts homologous sequence restricted by ECORV enzyme were designed to amplify the full open reading frames of the three CmedPRs and then be infusion into an expression vector pT7Ts The extracted plasmids were linearized by digestion with EcoRI and used as templates to synthesize cRNAs using the mMESSAGE mMACHINE T7 Kit (Ambion, USA). .. The purified cRNAs were diluted with nuclease-free water at a concentration of 2 μg/μL and stored at −80°C.

    Sequencing:

    Article Title: Pheromone Receptors Help Cnaphalocrocis medinalis Avoid Competition in Rice Fields
    Article Snippet: Gene expression levels were analyzed using the 2-ΔΔCT method, where ΔCT = CT PR gene – CT actin gene and Δ Δ CT = ΔCT different tissues – ΔCT maximum (Livak and Schmittgen 2001). .. Vector construction and cRNA synthesisThe specific primers with the Kozak consensus sequence and pT7Ts homologous sequence restricted by ECORV enzyme were designed to amplify the full open reading frames of the three CmedPRs and then be infusion into an expression vector pT7Ts The extracted plasmids were linearized by digestion with EcoRI and used as templates to synthesize cRNAs using the mMESSAGE mMACHINE T7 Kit (Ambion, USA). .. The purified cRNAs were diluted with nuclease-free water at a concentration of 2 μg/μL and stored at −80°C.

    Expressing:

    Article Title: Pheromone Receptors Help Cnaphalocrocis medinalis Avoid Competition in Rice Fields
    Article Snippet: Gene expression levels were analyzed using the 2-ΔΔCT method, where ΔCT = CT PR gene – CT actin gene and Δ Δ CT = ΔCT different tissues – ΔCT maximum (Livak and Schmittgen 2001). .. Vector construction and cRNA synthesisThe specific primers with the Kozak consensus sequence and pT7Ts homologous sequence restricted by ECORV enzyme were designed to amplify the full open reading frames of the three CmedPRs and then be infusion into an expression vector pT7Ts The extracted plasmids were linearized by digestion with EcoRI and used as templates to synthesize cRNAs using the mMESSAGE mMACHINE T7 Kit (Ambion, USA). .. The purified cRNAs were diluted with nuclease-free water at a concentration of 2 μg/μL and stored at −80°C.

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    Thermo Fisher ecorv restriction enzyme
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Ecorv Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction enzyme/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction enzyme - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher ecorv restriction enzymes
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Ecorv Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction enzymes/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction enzymes - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

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    Electrophoresis of RFLP products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the EcoRV enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans

    doi:

    Figure Lengend Snippet: Electrophoresis of RFLP products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the EcoRV enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker

    Article Snippet: After PCR and obtaining 114 bp sequences, for detecting D835 mutation based on RFLP procedure, products were incubated overnight at 37 °C with the EcoRV restriction enzyme (Fermentas).

    Techniques: Electrophoresis, Mutagenesis, Positive Control, Sequencing, Marker