ecori new england biolabs  (New England Biolabs)


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  • 99
    Name:
    EcoRI
    Description:
    EcoRI 50 000 units
    Catalog Number:
    r0101l
    Price:
    249
    Size:
    50 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs ecori new england biolabs
    EcoRI
    EcoRI 50 000 units
    https://www.bioz.com/result/ecori new england biolabs/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecori new england biolabs - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    endonuclease digestion
    southern blotting genomic dna

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    Related Articles

    Clone Assay:

    Article Title: Generation of recombinant Orf virus using an enhanced green fluorescent protein reporter gene as a selectable marker
    Article Snippet: .. The products were then cloned into vector pZIPPY-neo/gus [ ], which had been linearized with EcoRI and NotI (New England Biolabs, Ipswich, MA, USA) to generate the novel transfer vector pSPV-EGFP (Figure ). .. The plasmid was propagated in Escherichia coli strain Top10 (Invitrogen, Carlsbad, CA, USA).

    Amplification:

    Article Title: Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino
    Article Snippet: .. Mthylation Sensitive Amplified Polymorphism Analysis The MSAP technique was applied to the DNA pools from three diploid lines and three tetraploid lines C. lavandulifolium They were digested with either EcoR I and Hpa II or EcoR I and Msp I (NEB) at 37 °C for 12 h. The digested fragments were ligated to 5 pmol EcoR I adaptor and 50 pmol Hpa II/Msp I adaptor by incubation with 4 U T4 DNA polymerase (NEB) at 16 °C for 4 has described for the AFLP method. ..

    Modification:

    Article Title: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
    Article Snippet: .. Construction of modified pB42 vectors, and preparation for ligation to insert DNA Plasmid pB42 was digested with XhoI and EcoRI to completion, and precipitated and digested with CIP (NEB). .. This vector was then split into three reactions, where three pairs of oligos were added to form the new multiple cloning sites.

    Ligation:

    Article Title: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
    Article Snippet: .. Construction of modified pB42 vectors, and preparation for ligation to insert DNA Plasmid pB42 was digested with XhoI and EcoRI to completion, and precipitated and digested with CIP (NEB). .. This vector was then split into three reactions, where three pairs of oligos were added to form the new multiple cloning sites.

    Polymerase Chain Reaction:

    Article Title: Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates
    Article Snippet: .. EcoRI, Eco91l, HindIII and PstI, for complete digestion with the recommended RE buffers in separate PCR tubes in the following reaction volume: 7 µl nuclease-free water, 10 µl DNA, 2 µl 10× RE buffer, 1 µl (10 U) EcoRI (New England Biolabs)/1 µl (10 U) Eco91l (Fermentas) / 1 µl (10 U) HindIII (Fermentas) / 1 µl (10 U) PstI (Fermentas). ..

    Incubation:

    Article Title: Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino
    Article Snippet: .. Mthylation Sensitive Amplified Polymorphism Analysis The MSAP technique was applied to the DNA pools from three diploid lines and three tetraploid lines C. lavandulifolium They were digested with either EcoR I and Hpa II or EcoR I and Msp I (NEB) at 37 °C for 12 h. The digested fragments were ligated to 5 pmol EcoR I adaptor and 50 pmol Hpa II/Msp I adaptor by incubation with 4 U T4 DNA polymerase (NEB) at 16 °C for 4 has described for the AFLP method. ..

    Sequencing:

    Article Title: Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon
    Article Snippet: .. The authenticity of the plasmid construction was verified by EcoRI, AgeI and AscI (New England Biolabs) digestion (Fig. ) and by sequencing as previously described [ ]. .. This information indicated that eight substitutions were present in the RC coding region compared to the nucleotide sequence of passage 20 of the parental strain SAVH20/03 (Table ).

    Plasmid Preparation:

    Article Title: Deletion of the Clostridium thermocellum recA gene reveals that it is required for thermophilic plasmid replication but not plasmid integration at homologous DNA sequences
    Article Snippet: .. The colonies were picked into 10 mL LB with 50 μg/mL apramycin, and the plasmid DNA was extracted using a Miniprep kit (Qiagen, Valencia, CA, USA) and screened with restriction enzymes EcoRI and AvaI for pJGW92 and NcoI and AvaI for pJGW93 (NEB). .. The National Center for Biotechnology Information (NCBI) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to search for homologous proteins to known RecA, LexA, and DNA Pol V in the Clostridium thermocellum DSM 1313 genome.

    Article Title: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
    Article Snippet: .. Construction of modified pB42 vectors, and preparation for ligation to insert DNA Plasmid pB42 was digested with XhoI and EcoRI to completion, and precipitated and digested with CIP (NEB). .. This vector was then split into three reactions, where three pairs of oligos were added to form the new multiple cloning sites.

    Article Title: Generation of recombinant Orf virus using an enhanced green fluorescent protein reporter gene as a selectable marker
    Article Snippet: .. The products were then cloned into vector pZIPPY-neo/gus [ ], which had been linearized with EcoRI and NotI (New England Biolabs, Ipswich, MA, USA) to generate the novel transfer vector pSPV-EGFP (Figure ). .. The plasmid was propagated in Escherichia coli strain Top10 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon
    Article Snippet: .. The authenticity of the plasmid construction was verified by EcoRI, AgeI and AscI (New England Biolabs) digestion (Fig. ) and by sequencing as previously described [ ]. .. This information indicated that eight substitutions were present in the RC coding region compared to the nucleotide sequence of passage 20 of the parental strain SAVH20/03 (Table ).

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  • 99
    New England Biolabs ecorv restriction endonuclease
    pH dependence of the <t>EcoRV</t> specific-nonspecific free binding energy difference. The pH dependence of ln(K nsp-sp ) is shown for the range 5.5 to 8.0. Mixtures of EcoRV, the 310 bp specific site <t>DNA</t> fragment, and the nonspecific oligonucleotide competitor
    Ecorv Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2 article reviews
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    80
    New England Biolabs hin cii eco ri
    Diagrammatic representation of the liver-derived gp70 cDNA and viral env ) is 99% homologous to that of the infectious NZB xenotropic virus env ), except for several base changes clustered near the gp70/p15E cleavage site and in the 3′ flanking region and <t>LTR</t> ( ), which are reflected by the indicated differences in restriction sites. The SFFV env gene ( ) is a product of natural recombination between endogenous polytropic and exogenous Friend ecotropic viruses with a large in-frame deletion (▿) encompassing the 3′ portion of gp70- and the 5′ portion of p15E-encoding regions. Dashed lines represent vector-derived sequences. A, Acc I; B, Bam HI; E, Eco RI; Ha, Hae II; Hc, Hin <t>cII;</t> Hd, Hin dIII; K, Kpn I; S, Sma I; V, Eco RV; X, Bst XI; AAAAA, poly(A) tail.
    Hin Cii Eco Ri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    New England Biolabs eco ri mtase
    Continuous monitoring of methyltransferases. To perform the methyltransferase assay, a 100 μl reaction typically contained the following assay components: 3.5 μg purified recombinant Mtn, 1 μg XOD (10 units/mg), 0.2 μg HRP (300 units/mg), 0.1 mM Amplex Red, 5 mM MgCl 2 , 0.5 mM SAM (32 mM stock), 1 mM salicylic acid or ~14 nM lambda <t>DNA</t> (0.5 mg/ml), 1-3 μg SA <t>MTase</t> or 80 units Eco RI MTase, and 50 mM Tris-HCl (pH 7.5) or 50 mM potassium phosphate buffer (pH 7.5). For SA MTase, the mixture was additionally supplemented with 1 mM KCl to stimulate activity. Reactions were initiated either with addition of the substrate or enzyme. Fluorescence output was monitored in 96-well microplates at 590 nm with excitation set at 530 nm. The reactions were incubated at 30°C without shaking for up to 60 mins. (A) Methyltransferase activity of Eco RI MTase in the presence of lambda DNA and SAM. (B) Confirmation of lambda DNA methylation by agarose gel electrophoresis. (C) Methyltransferase activity of SA MTase in the presence of salicylic acid (SA) and SAM. (D) Confirmation of the synthesis of methyl salicylate by HPLC.
    Eco Ri Mtase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    pH dependence of the EcoRV specific-nonspecific free binding energy difference. The pH dependence of ln(K nsp-sp ) is shown for the range 5.5 to 8.0. Mixtures of EcoRV, the 310 bp specific site DNA fragment, and the nonspecific oligonucleotide competitor

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: pH dependence of the EcoRV specific-nonspecific free binding energy difference. The pH dependence of ln(K nsp-sp ) is shown for the range 5.5 to 8.0. Mixtures of EcoRV, the 310 bp specific site DNA fragment, and the nonspecific oligonucleotide competitor

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Binding Assay

    Kinetics of the EcoRV-DNA complex formation. The kinetics of DNA-protein complex formation was measured using the self-cleavage assay at different conditions of pH: pH 6.3 (▲); pH 7.6 (■). The binding of the EcoRV proceeds in at least

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: Kinetics of the EcoRV-DNA complex formation. The kinetics of DNA-protein complex formation was measured using the self-cleavage assay at different conditions of pH: pH 6.3 (▲); pH 7.6 (■). The binding of the EcoRV proceeds in at least

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Cleavage Assay, Binding Assay

    A direct comparison of EcoRV-DNA binding analyzed by the gel mobility shift assay and by the self-cleavage assay. (A ) A gel image is shown illustrating a direct comparison of the EcoRV-DNA binding by the gel mobility shift assay (left), and by the self-cleavage

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: A direct comparison of EcoRV-DNA binding analyzed by the gel mobility shift assay and by the self-cleavage assay. (A ) A gel image is shown illustrating a direct comparison of the EcoRV-DNA binding by the gel mobility shift assay (left), and by the self-cleavage

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Binding Assay, Mobility Shift, Cleavage Assay

    Equilibrium competition between specific and nonspecific DNA sequences for the EcoRV binding. Mixtures of EcoRV, the 310 bp DNA fragment with a specific recognition site, and nonspecific oligonucleotide competitor were incubated at 20 °C overnight

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: Equilibrium competition between specific and nonspecific DNA sequences for the EcoRV binding. Mixtures of EcoRV, the 310 bp DNA fragment with a specific recognition site, and nonspecific oligonucleotide competitor were incubated at 20 °C overnight

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Binding Assay, Incubation

    The dependence of the EcoRV specific-nonspecific binding free energy difference, ln(K nsp-sp ) in the units of kT, on solute osmolal concentration is shown for four neutral osmolytes. Mixtures of the specific site DNA fragment, nonspecific oligonucleotide,

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: The dependence of the EcoRV specific-nonspecific binding free energy difference, ln(K nsp-sp ) in the units of kT, on solute osmolal concentration is shown for four neutral osmolytes. Mixtures of the specific site DNA fragment, nonspecific oligonucleotide,

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Binding Assay, Concentration Assay

    The pH dependence of Knsp-sp for EcoRV-DNA binding

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: The pH dependence of Knsp-sp for EcoRV-DNA binding

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Binding Assay

    The dependence of the EcoRV specific-nonspecific binding free energy difference on triethylene glycol concentration is shown for different pH values. Mixtures of EcoRV, the 310 bp specific site DNA fragment, and the nonspecific oligonucleotide competitor

    Journal: The FEBS journal

    Article Title: Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV

    doi: 10.1111/j.1742-4658.2011.08198.x

    Figure Lengend Snippet: The dependence of the EcoRV specific-nonspecific binding free energy difference on triethylene glycol concentration is shown for different pH values. Mixtures of EcoRV, the 310 bp specific site DNA fragment, and the nonspecific oligonucleotide competitor

    Article Snippet: DNA binding and cleavage experiments were performed with highly purified EcoRV restriction endonuclease (described below) or with commercial EcoRV sample purchased from New England Biolabs.

    Techniques: Binding Assay, Concentration Assay

    Diagrammatic representation of the liver-derived gp70 cDNA and viral env ) is 99% homologous to that of the infectious NZB xenotropic virus env ), except for several base changes clustered near the gp70/p15E cleavage site and in the 3′ flanking region and LTR ( ), which are reflected by the indicated differences in restriction sites. The SFFV env gene ( ) is a product of natural recombination between endogenous polytropic and exogenous Friend ecotropic viruses with a large in-frame deletion (▿) encompassing the 3′ portion of gp70- and the 5′ portion of p15E-encoding regions. Dashed lines represent vector-derived sequences. A, Acc I; B, Bam HI; E, Eco RI; Ha, Hae II; Hc, Hin cII; Hd, Hin dIII; K, Kpn I; S, Sma I; V, Eco RV; X, Bst XI; AAAAA, poly(A) tail.

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Diagrammatic representation of the liver-derived gp70 cDNA and viral env ) is 99% homologous to that of the infectious NZB xenotropic virus env ), except for several base changes clustered near the gp70/p15E cleavage site and in the 3′ flanking region and LTR ( ), which are reflected by the indicated differences in restriction sites. The SFFV env gene ( ) is a product of natural recombination between endogenous polytropic and exogenous Friend ecotropic viruses with a large in-frame deletion (▿) encompassing the 3′ portion of gp70- and the 5′ portion of p15E-encoding regions. Dashed lines represent vector-derived sequences. A, Acc I; B, Bam HI; E, Eco RI; Ha, Hae II; Hc, Hin cII; Hd, Hin dIII; K, Kpn I; S, Sma I; V, Eco RV; X, Bst XI; AAAAA, poly(A) tail.

    Article Snippet: The Hin cII- Sma I fragment harboring the entire env gene and portions of the pol and LTR from pNZB9-1 was reconstructed in pBluescript-KS(+) vector from purified Hin cII- Eco RI and Eco RI- Sma I fragments (Fig. ), and the unique Acc I site was replaced with a Bam HI linker (New England Biolabs).

    Techniques: Derivative Assay, Plasmid Preparation

    Continuous monitoring of methyltransferases. To perform the methyltransferase assay, a 100 μl reaction typically contained the following assay components: 3.5 μg purified recombinant Mtn, 1 μg XOD (10 units/mg), 0.2 μg HRP (300 units/mg), 0.1 mM Amplex Red, 5 mM MgCl 2 , 0.5 mM SAM (32 mM stock), 1 mM salicylic acid or ~14 nM lambda DNA (0.5 mg/ml), 1-3 μg SA MTase or 80 units Eco RI MTase, and 50 mM Tris-HCl (pH 7.5) or 50 mM potassium phosphate buffer (pH 7.5). For SA MTase, the mixture was additionally supplemented with 1 mM KCl to stimulate activity. Reactions were initiated either with addition of the substrate or enzyme. Fluorescence output was monitored in 96-well microplates at 590 nm with excitation set at 530 nm. The reactions were incubated at 30°C without shaking for up to 60 mins. (A) Methyltransferase activity of Eco RI MTase in the presence of lambda DNA and SAM. (B) Confirmation of lambda DNA methylation by agarose gel electrophoresis. (C) Methyltransferase activity of SA MTase in the presence of salicylic acid (SA) and SAM. (D) Confirmation of the synthesis of methyl salicylate by HPLC.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases

    doi: 10.3389/fbioe.2018.00146

    Figure Lengend Snippet: Continuous monitoring of methyltransferases. To perform the methyltransferase assay, a 100 μl reaction typically contained the following assay components: 3.5 μg purified recombinant Mtn, 1 μg XOD (10 units/mg), 0.2 μg HRP (300 units/mg), 0.1 mM Amplex Red, 5 mM MgCl 2 , 0.5 mM SAM (32 mM stock), 1 mM salicylic acid or ~14 nM lambda DNA (0.5 mg/ml), 1-3 μg SA MTase or 80 units Eco RI MTase, and 50 mM Tris-HCl (pH 7.5) or 50 mM potassium phosphate buffer (pH 7.5). For SA MTase, the mixture was additionally supplemented with 1 mM KCl to stimulate activity. Reactions were initiated either with addition of the substrate or enzyme. Fluorescence output was monitored in 96-well microplates at 590 nm with excitation set at 530 nm. The reactions were incubated at 30°C without shaking for up to 60 mins. (A) Methyltransferase activity of Eco RI MTase in the presence of lambda DNA and SAM. (B) Confirmation of lambda DNA methylation by agarose gel electrophoresis. (C) Methyltransferase activity of SA MTase in the presence of salicylic acid (SA) and SAM. (D) Confirmation of the synthesis of methyl salicylate by HPLC.

    Article Snippet: During the assay, a linear increase in fluorescence intensity was observed upon addition of Eco RI MTAse with lambda DNA as substrate at a concentration of 96 nM (Figure ).

    Techniques: Purification, Recombinant, Lambda DNA Preparation, Activity Assay, Fluorescence, Incubation, Methylation, Agarose Gel Electrophoresis, High Performance Liquid Chromatography

    Continuous monitoring of methyltransferases. To perform the methyltransferase assay, a 100 μl reaction typically contained the following assay components: 3.5 μg purified recombinant Mtn, 1 μg XOD (10 units/mg), 0.2 μg HRP (300 units/mg), 0.1 mM Amplex Red, 5 mM MgCl 2 , 0.5 mM SAM (32 mM stock), 1 mM salicylic acid or ~14 nM lambda DNA (0.5 mg/ml), 1-3 μg SA MTase or 80 units Eco RI MTase, and 50 mM Tris-HCl (pH 7.5) or 50 mM potassium phosphate buffer (pH 7.5). For SA MTase, the mixture was additionally supplemented with 1 mM KCl to stimulate activity. Reactions were initiated either with addition of the substrate or enzyme. Fluorescence output was monitored in 96-well microplates at 590 nm with excitation set at 530 nm. The reactions were incubated at 30°C without shaking for up to 60 mins. (A) Methyltransferase activity of Eco RI MTase in the presence of lambda DNA and SAM. (B) Confirmation of lambda DNA methylation by agarose gel electrophoresis. (C) Methyltransferase activity of SA MTase in the presence of salicylic acid (SA) and SAM. (D) Confirmation of the synthesis of methyl salicylate by HPLC.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases

    doi: 10.3389/fbioe.2018.00146

    Figure Lengend Snippet: Continuous monitoring of methyltransferases. To perform the methyltransferase assay, a 100 μl reaction typically contained the following assay components: 3.5 μg purified recombinant Mtn, 1 μg XOD (10 units/mg), 0.2 μg HRP (300 units/mg), 0.1 mM Amplex Red, 5 mM MgCl 2 , 0.5 mM SAM (32 mM stock), 1 mM salicylic acid or ~14 nM lambda DNA (0.5 mg/ml), 1-3 μg SA MTase or 80 units Eco RI MTase, and 50 mM Tris-HCl (pH 7.5) or 50 mM potassium phosphate buffer (pH 7.5). For SA MTase, the mixture was additionally supplemented with 1 mM KCl to stimulate activity. Reactions were initiated either with addition of the substrate or enzyme. Fluorescence output was monitored in 96-well microplates at 590 nm with excitation set at 530 nm. The reactions were incubated at 30°C without shaking for up to 60 mins. (A) Methyltransferase activity of Eco RI MTase in the presence of lambda DNA and SAM. (B) Confirmation of lambda DNA methylation by agarose gel electrophoresis. (C) Methyltransferase activity of SA MTase in the presence of salicylic acid (SA) and SAM. (D) Confirmation of the synthesis of methyl salicylate by HPLC.

    Article Snippet: For this purpose, we employed Eco RI MTase which is known to methylate the DNA sequence, GAATTC, of double-stranded linear DNA (Rubin and Modrich, ).

    Techniques: Purification, Recombinant, Lambda DNA Preparation, Activity Assay, Fluorescence, Incubation, Methylation, Agarose Gel Electrophoresis, High Performance Liquid Chromatography