ecoo109i  (New England Biolabs)


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  • 94
    Name:
    EcoO109I
    Description:
    EcoO109I 2 000 units
    Catalog Number:
    r0503s
    Price:
    66
    Size:
    2 000 units
    Category:
    Restriction Enzymes
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    New England Biolabs ecoo109i
    EcoO109I
    EcoO109I 2 000 units
    https://www.bioz.com/result/ecoo109i/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecoo109i - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Ligation:

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

    Construct:

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

    Article Title: A construct with fluorescent indicators for conditional expression of miRNA
    Article Snippet: .. Plasmid vectors The pEGFP-U6 short hairpin expression vector (Figure ) was constructed by inserting the U6 promoter derived from BSENU6 [ ] into pEGFP-N1 (Clontech) at the EcoO109I site (all restriction enzymes were obtained from New England Biolabs unless indicated otherwise). .. The U6 promoter fragment was amplified by PCR with introduction of EcoO109I sites at both ends using a pair of PCR primers (Forward: 5'-CAAGGCCCTTTGACGTCAATGGGAGTTTGTTTTGG-3'; Reverse: 5'-TTAGGGCCCGAGCGGATAACAATTTCACACAGGAA-3').

    Mouse Assay:

    Article Title: Alternating Hemiplegia of Childhood-Related Neural and Behavioural Phenotypes in Na+,K+-ATPase ?3 Missense Mutant Mice
    Article Snippet: .. Mice used in the present study were bred from C57BL/6NCrl females (Charles River) and Myk /+ males, and were genotyped by the presence of an Eco O109I (New England BioLabs) restriction site using polymerase chain reaction (PCR) primers F, 5′-CTG CCG GAA ATA CAA TAC TGA-3′ and R, 5′-ATA AAT ACC CCA CCA CTG AGC-3′ . ..

    Electrophoresis:

    Article Title: Deficiencies of Human Complement Component C4a and C4b and Heterozygosity in Length Variants of RP-C4-CYP21-TNX (Rccx) Modules in Caucasians
    Article Snippet: .. DNA fragments were resolved by electrophoresis of 0.8% agarose gels except for EcoO109I- and NlaIV-digested DNAs, for which 1.2% gels were used. .. Southern blot analysis was performed as described previously .

    Polymerase Chain Reaction:

    Article Title: Alternating Hemiplegia of Childhood-Related Neural and Behavioural Phenotypes in Na+,K+-ATPase ?3 Missense Mutant Mice
    Article Snippet: .. Mice used in the present study were bred from C57BL/6NCrl females (Charles River) and Myk /+ males, and were genotyped by the presence of an Eco O109I (New England BioLabs) restriction site using polymerase chain reaction (PCR) primers F, 5′-CTG CCG GAA ATA CAA TAC TGA-3′ and R, 5′-ATA AAT ACC CCA CCA CTG AGC-3′ . ..

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

    Article Title: Ki-67 Contributes to Normal Cell Cycle Progression and Inactive X Heterochromatin in p21 Checkpoint-Proficient Human Cells
    Article Snippet: .. PCR products (1,523 bp) were further digested with the EcoO109I restriction enzyme (New England BioLabs). ..

    Article Title: DNA Sequence Analysis of SLC26A5, Encoding Prestin, in a Patient-Control Cohort: Identification of Fourteen Novel DNA Sequence Variations
    Article Snippet: .. PCR fragments containing position g.53884 were digested with restriction enzyme EcoO109I (New England Biolabs, Ipswich, MA, USA). .. When the consensus sequence was present, the digestion resulted in two fragments of 420 bp and 180 bp.

    Plasmid Preparation:

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

    Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
    Article Snippet: .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs). .. A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site.

    Article Title: A construct with fluorescent indicators for conditional expression of miRNA
    Article Snippet: .. Plasmid vectors The pEGFP-U6 short hairpin expression vector (Figure ) was constructed by inserting the U6 promoter derived from BSENU6 [ ] into pEGFP-N1 (Clontech) at the EcoO109I site (all restriction enzymes were obtained from New England Biolabs unless indicated otherwise). .. The U6 promoter fragment was amplified by PCR with introduction of EcoO109I sites at both ends using a pair of PCR primers (Forward: 5'-CAAGGCCCTTTGACGTCAATGGGAGTTTGTTTTGG-3'; Reverse: 5'-TTAGGGCCCGAGCGGATAACAATTTCACACAGGAA-3').

    Expressing:

    Article Title: A construct with fluorescent indicators for conditional expression of miRNA
    Article Snippet: .. Plasmid vectors The pEGFP-U6 short hairpin expression vector (Figure ) was constructed by inserting the U6 promoter derived from BSENU6 [ ] into pEGFP-N1 (Clontech) at the EcoO109I site (all restriction enzymes were obtained from New England Biolabs unless indicated otherwise). .. The U6 promoter fragment was amplified by PCR with introduction of EcoO109I sites at both ends using a pair of PCR primers (Forward: 5'-CAAGGCCCTTTGACGTCAATGGGAGTTTGTTTTGG-3'; Reverse: 5'-TTAGGGCCCGAGCGGATAACAATTTCACACAGGAA-3').

    CTG Assay:

    Article Title: Alternating Hemiplegia of Childhood-Related Neural and Behavioural Phenotypes in Na+,K+-ATPase ?3 Missense Mutant Mice
    Article Snippet: .. Mice used in the present study were bred from C57BL/6NCrl females (Charles River) and Myk /+ males, and were genotyped by the presence of an Eco O109I (New England BioLabs) restriction site using polymerase chain reaction (PCR) primers F, 5′-CTG CCG GAA ATA CAA TAC TGA-3′ and R, 5′-ATA AAT ACC CCA CCA CTG AGC-3′ . ..

    Cellular Antioxidant Activity Assay:

    Article Title: Alternating Hemiplegia of Childhood-Related Neural and Behavioural Phenotypes in Na+,K+-ATPase ?3 Missense Mutant Mice
    Article Snippet: .. Mice used in the present study were bred from C57BL/6NCrl females (Charles River) and Myk /+ males, and were genotyped by the presence of an Eco O109I (New England BioLabs) restriction site using polymerase chain reaction (PCR) primers F, 5′-CTG CCG GAA ATA CAA TAC TGA-3′ and R, 5′-ATA AAT ACC CCA CCA CTG AGC-3′ . ..

    Derivative Assay:

    Article Title: A construct with fluorescent indicators for conditional expression of miRNA
    Article Snippet: .. Plasmid vectors The pEGFP-U6 short hairpin expression vector (Figure ) was constructed by inserting the U6 promoter derived from BSENU6 [ ] into pEGFP-N1 (Clontech) at the EcoO109I site (all restriction enzymes were obtained from New England Biolabs unless indicated otherwise). .. The U6 promoter fragment was amplified by PCR with introduction of EcoO109I sites at both ends using a pair of PCR primers (Forward: 5'-CAAGGCCCTTTGACGTCAATGGGAGTTTGTTTTGG-3'; Reverse: 5'-TTAGGGCCCGAGCGGATAACAATTTCACACAGGAA-3').

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  • 94
    New England Biolabs ecoo109i restriction enzyme
    Validation of specificity and effectiveness of Ki-67 depletion reagents. (A) CRISPR/Cas9-based strategy for generating siRNA-resistant mutations in the endogenous Ki-67 gene. The si-Ki-67 target (green letters) and sgRNA-directed cleavage site (triangle) are indicated in the upper diagram of the endogenous locus. Altered nucleotides (red) and the novel <t>EcoO109I</t> restriction site are shown in the lower diagram of the repair template. (B) DNA sequence analysis of PCR products from wild-type hTERT-RPE1 cells and si-Ki-67-resistant clone 7. (C) EcoO109I digestion of the same PCR products sequenced for panel B. (D) Immunoblot analysis of clone 7 treated with the indicated reagents. (E) RT-qPCR analysis of clone 7. (F to L) Immunoblot analyses of the indicated cell lines treated with the indicated RNA depletion reagents.
    Ecoo109i Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecoo109i restriction enzyme/product/New England Biolabs
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ecoo109i restriction enzyme - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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    Validation of specificity and effectiveness of Ki-67 depletion reagents. (A) CRISPR/Cas9-based strategy for generating siRNA-resistant mutations in the endogenous Ki-67 gene. The si-Ki-67 target (green letters) and sgRNA-directed cleavage site (triangle) are indicated in the upper diagram of the endogenous locus. Altered nucleotides (red) and the novel EcoO109I restriction site are shown in the lower diagram of the repair template. (B) DNA sequence analysis of PCR products from wild-type hTERT-RPE1 cells and si-Ki-67-resistant clone 7. (C) EcoO109I digestion of the same PCR products sequenced for panel B. (D) Immunoblot analysis of clone 7 treated with the indicated reagents. (E) RT-qPCR analysis of clone 7. (F to L) Immunoblot analyses of the indicated cell lines treated with the indicated RNA depletion reagents.

    Journal: Molecular and Cellular Biology

    Article Title: Ki-67 Contributes to Normal Cell Cycle Progression and Inactive X Heterochromatin in p21 Checkpoint-Proficient Human Cells

    doi: 10.1128/MCB.00569-16

    Figure Lengend Snippet: Validation of specificity and effectiveness of Ki-67 depletion reagents. (A) CRISPR/Cas9-based strategy for generating siRNA-resistant mutations in the endogenous Ki-67 gene. The si-Ki-67 target (green letters) and sgRNA-directed cleavage site (triangle) are indicated in the upper diagram of the endogenous locus. Altered nucleotides (red) and the novel EcoO109I restriction site are shown in the lower diagram of the repair template. (B) DNA sequence analysis of PCR products from wild-type hTERT-RPE1 cells and si-Ki-67-resistant clone 7. (C) EcoO109I digestion of the same PCR products sequenced for panel B. (D) Immunoblot analysis of clone 7 treated with the indicated reagents. (E) RT-qPCR analysis of clone 7. (F to L) Immunoblot analyses of the indicated cell lines treated with the indicated RNA depletion reagents.

    Article Snippet: PCR products (1,523 bp) were further digested with the EcoO109I restriction enzyme (New England BioLabs).

    Techniques: CRISPR, Sequencing, Polymerase Chain Reaction, Quantitative RT-PCR