econo pac 10 dg disposable chromatography column  (Bio-Rad)

 
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    Name:
    Econo Pac 10DG Desalting Columns
    Description:
    Pkg of 30 10 ml packed resin 20 ml reservoir prepacked gravity flow chromatography column includes upper frit
    Catalog Number:
    7322010
    Price:
    None
    Category:
    Bio Gel P Polyacrylamide Gel
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    Structured Review

    Bio-Rad econo pac 10 dg disposable chromatography column
    Econo Pac 10DG Desalting Columns
    Pkg of 30 10 ml packed resin 20 ml reservoir prepacked gravity flow chromatography column includes upper frit
    https://www.bioz.com/result/econo pac 10 dg disposable chromatography column/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    econo pac 10 dg disposable chromatography column - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Structural Basis for Iron-mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases ‡
    Article Snippet: Paragraph title: Cloning, Overexpression and Purification ... The eluted samples were buffer exchanged into 10 mM Tris, 50 mM NaCl, pH 8.0 using an Econo-Pac 10DG desalting column (Bio-Rad).

    Centrifugation:

    Article Title: A Cytosolic Serine Endopeptidase from Trypanosoma cruzi Is Required for the Generation of Ca2+ Signaling in Mammalian Cells
    Article Snippet: .. Precipitated protein was recovered by centrifugation at 15,000 g for 30 min, and pellets were resuspended in PBS2+ containing 20 μM E-64, filtered through a 0.22-μm filter unit ( Millipore Products Division, Bedford, MA) and desalted on an Econo-Pac® 10DG column (BioRad Laboratories, Hercules, CA) equilibrated in PBS2+ . ..

    Amplification:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: After amplification of the pCRII-TOPO-TRAIL vector DNA, the TRAIL gene was cut out with NotI and inserted into the NotI site of the vector pAdtet to create pAdtet-TRAIL. .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Autoradiography:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13. .. 10 μL of each elution fraction was electrophoresed on a 12% SDS-PAGE gel under reducing conditions and evaluated by autoradiography using a BAS-5000 phosphorimager (Fujifilm).

    Construct:

    Article Title: Efficient one-step radiolabeling of monoclonal antibodies to high specific activity with Actinium-225 for alpha-particle radioimmunotherapy of cancer
    Article Snippet: To this were added 2 M tetramethyl ammonium acetate (TMAA) buffer (25 μl), 150 g/l L-ascorbic acid (10 μl), and the appropriate antibody construct (100 μg). pH of the reaction was determined by spotting 1 μl of the reaction mixture onto Hydrion pH paper range 5.0-9.0 (Sigma-Aldrich); pH of a typical reaction was 5.8. .. The reaction was then quenched with 50 mM DTPA (20 μl) and purified using an Econo-Pac 10DG desalting column (Bio-Rad) that had been equilibrated previously with 1% Human Serum Albumin (HSA).

    Incubation:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: The supernatant was incubated with 6 mL Strep-tag Tactin slurry (50% Strep-tag Tactin in 50 mM potassium phosphate buffer, pH 8, 150 mM NaCl, 10% glycerol), for one hour on a rotary shaker at 4°C. .. LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min).

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: After adding A-overhangs by incubation with Taq polymerase, the PCR fragment was inserted into the expression vector pCRII-TOPO, which possesses T-overhangs, to create pCRII-TOPO-TRAIL. .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Article Title: Structural Basis for Iron-mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases ‡
    Article Snippet: The eluted samples were buffer exchanged into 10 mM Tris, 50 mM NaCl, pH 8.0 using an Econo-Pac 10DG desalting column (Bio-Rad). .. The purified protein was concentrated to 10 mg/mL using an Amicon Ultra-0.5 concentrator (10 kDa MWCO filter, Millipore) and incubated at room temperature for 1 h with 2 mM NAD, 2 mM glycine, 0.5 mM ferrous ammonium sulfate (FeAS), and 2 mM dithiothreitol.

    Article Title: The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis
    Article Snippet: The protein solution was applied to an Econo-Pac 10DG desalting column (Bio-Rad) and exchanged into 50 mM EDTA, 50 mM Tris–Cl, pH 8.0 buffer. .. The de-metalated protein was again concentrated to 2.0 ml by ultracentrifugation and stored at 4 o C. PntC activity was monitored by 31 P NMR after 2 h incubation at room temperature in 50 mM Tris–Cl pH 8.0 containing 7.0 mM CTP, 3 mM AEP, 5.5 µM Tde1415, and 7.0 mM of divalent metal (MgCl2 , CaCl2 , or ZnCl2 ).

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: Approximately 100 µl of 0.05% sodium azide/PBS was added and allowed to flow through, after which the column was closed at the bottom, 500 µl of 0.05% sodium azide/PBS added, the top capped and incubated at room temperature for 1 h. Next, the column was washed with 12 ml of PBS and antiserum eluted (in 0.5 ml fractions) by addition of 4 ml of 100 mM glycine-HCl (pH 2.7). .. The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Stripping Membranes:

    Article Title: Efficient one-step radiolabeling of monoclonal antibodies to high specific activity with Actinium-225 for alpha-particle radioimmunotherapy of cancer
    Article Snippet: After this a small aliquot was spotted on a strip of instant thin layer chromatography-silica gel (iTLC-SG) paper to determine the extent of incorporation of actinium onto protein (details in ). .. The reaction was then quenched with 50 mM DTPA (20 μl) and purified using an Econo-Pac 10DG desalting column (Bio-Rad) that had been equilibrated previously with 1% Human Serum Albumin (HSA).

    Activity Assay:

    Article Title: A Cytosolic Serine Endopeptidase from Trypanosoma cruzi Is Required for the Generation of Ca2+ Signaling in Mammalian Cells
    Article Snippet: Precipitated protein was recovered by centrifugation at 15,000 g for 30 min, and pellets were resuspended in PBS2+ containing 20 μM E-64, filtered through a 0.22-μm filter unit ( Millipore Products Division, Bedford, MA) and desalted on an Econo-Pac® 10DG column (BioRad Laboratories, Hercules, CA) equilibrated in PBS2+ . .. Peptidase activity was assayed using the synthetic substrate Z-phenylalanyl-arginyl-7amino-4-methyl coumarin (Z-Phe-Arg-AMC) (Enzyme Systems Products, Livermore, CA) as described previously ( ).

    Article Title: The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis
    Article Snippet: The protein solution was applied to an Econo-Pac 10DG desalting column (Bio-Rad) and exchanged into 50 mM EDTA, 50 mM Tris–Cl, pH 8.0 buffer. .. The de-metalated protein was again concentrated to 2.0 ml by ultracentrifugation and stored at 4 o C. PntC activity was monitored by 31 P NMR after 2 h incubation at room temperature in 50 mM Tris–Cl pH 8.0 containing 7.0 mM CTP, 3 mM AEP, 5.5 µM Tde1415, and 7.0 mM of divalent metal (MgCl2 , CaCl2 , or ZnCl2 ).

    Article Title: Efficient one-step radiolabeling of monoclonal antibodies to high specific activity with Actinium-225 for alpha-particle radioimmunotherapy of cancer
    Article Snippet: In a typical one-step procedure, 225 Ac -nitrate (3.7 MBq) dissolved in 0.2 M HCl was added to a 1.0 ml Nunc vial and the activity was determined exactly using a dose calibrator. .. The reaction was then quenched with 50 mM DTPA (20 μl) and purified using an Econo-Pac 10DG desalting column (Bio-Rad) that had been equilibrated previously with 1% Human Serum Albumin (HSA).

    Expressing:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: After adding A-overhangs by incubation with Taq polymerase, the PCR fragment was inserted into the expression vector pCRII-TOPO, which possesses T-overhangs, to create pCRII-TOPO-TRAIL. .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: Protein Expression and Crystallization —IsdG-N7A and IsdI were expressed in E. coli BL21 (DE3) and purified as reported previously ( ). .. DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8.

    Crystallization Assay:

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: Protein Expression and Crystallization —IsdG-N7A and IsdI were expressed in E. coli BL21 (DE3) and purified as reported previously ( ). .. DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8.

    Over Expression:

    Article Title: Disruption of the Proton Relay Network in the Class 2 Dihydroorotate Dehydrogenase from Escherichia coli †
    Article Snippet: Paragraph title: Overexpression and Purification ... Before use, enzymes were exchanged into the appropriate buffer using Econo-Pac 10DG disposable desalting columns (Bio-Rad).

    Article Title: Structural Basis for Iron-mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases ‡
    Article Snippet: Paragraph title: Cloning, Overexpression and Purification ... The eluted samples were buffer exchanged into 10 mM Tris, 50 mM NaCl, pH 8.0 using an Econo-Pac 10DG desalting column (Bio-Rad).

    Derivative Assay:

    Article Title: Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
    Article Snippet: In the samples derived from the flask production trials, all buffer exchanges were conducted using the Spin-X UF concentrators (Corning, Corning, NY, USA) with a 5 kDa molecular weight cut-off PES (polyethersulfone) membrane. .. In the case of the bioreactor production trials, all buffer exchanges were conducted with the use of Econo-Pac 10DG columns (Bio Rad, Hercules, CA, USA) as per the manufacturer’s instructions.

    High Performance Liquid Chromatography:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: The HPLC-purified [18 F]SFB was concentrated to dryness on a rotary evaporator set at 35°C. .. 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13.

    Flow Cytometry:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: Subsequently, the material was loaded on a gravity flow column, and washed 5 times with two column volumes of resuspension buffer. .. LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min).

    Article Title: A Cytosolic Serine Endopeptidase from Trypanosoma cruzi Is Required for the Generation of Ca2+ Signaling in Mammalian Cells
    Article Snippet: The flow-through fraction from Con A–Sepharose (50 ml) was applied to a 10ml column of DEAE-cellulose equilibrated in PBS2+ . .. Precipitated protein was recovered by centrifugation at 15,000 g for 30 min, and pellets were resuspended in PBS2+ containing 20 μM E-64, filtered through a 0.22-μm filter unit ( Millipore Products Division, Bedford, MA) and desalted on an Econo-Pac® 10DG column (BioRad Laboratories, Hercules, CA) equilibrated in PBS2+ .

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: Approximately 100 µl of 0.05% sodium azide/PBS was added and allowed to flow through, after which the column was closed at the bottom, 500 µl of 0.05% sodium azide/PBS added, the top capped and incubated at room temperature for 1 h. Next, the column was washed with 12 ml of PBS and antiserum eluted (in 0.5 ml fractions) by addition of 4 ml of 100 mM glycine-HCl (pH 2.7). .. The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Chromatography:

    Article Title: Efficient one-step radiolabeling of monoclonal antibodies to high specific activity with Actinium-225 for alpha-particle radioimmunotherapy of cancer
    Article Snippet: After this a small aliquot was spotted on a strip of instant thin layer chromatography-silica gel (iTLC-SG) paper to determine the extent of incorporation of actinium onto protein (details in ). .. The reaction was then quenched with 50 mM DTPA (20 μl) and purified using an Econo-Pac 10DG desalting column (Bio-Rad) that had been equilibrated previously with 1% Human Serum Albumin (HSA).

    Buffer Exchange:

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: Using a buffer exchange column (Bio-Rad) they were then moved to coupling buffer (4 M Guanidine HCl, 100 mM Na phosphate pH 6.4, 0.05% Na-azide), protein concentration determined (Bio-Rad microassay) and coupled to beads (Aminolink resin; Pierce) to form an affinity column. .. The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Transferring:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: 100 µg of anti-CA125 scFv (2 mg/mL) in 50 mM borate buffer pH 8.3 was added to the dried [18 F]SFB and mixed well by adding an additional 450 μL of borate buffer to dissolve the two reactants prior to transferring the reaction mixture into a Protein LoBind 1.5 mL tube (Eppendorf, 13698794). .. 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13.

    SDS Page:

    Article Title: Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
    Article Snippet: Then, the binding buffer was changed to the elution buffer (20 mM, 500 mM NaCl, 500 mM imidazole pH 7.4) and 5 mL fractions were collected for SDS-PAGE analysis. .. In the case of the bioreactor production trials, all buffer exchanges were conducted with the use of Econo-Pac 10DG columns (Bio Rad, Hercules, CA, USA) as per the manufacturer’s instructions.

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13. .. 10 μL of each elution fraction was electrophoresed on a 12% SDS-PAGE gel under reducing conditions and evaluated by autoradiography using a BAS-5000 phosphorimager (Fujifilm).

    other:

    Article Title: Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide
    Article Snippet: Removal of Hydroxyprolyl Peptides from FBS-1 An Econo-Pac 10DG column (Bio-Rad Laboratories, Hercules, CA, USA) was pre-equilibrated with the DMEM.

    Protein Concentration:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min). .. Fractions were analyzed on a 12% SDS polyacrylamide gel, stained with Coomassie Brilliant Blue, and the protein concentration and purity was assessed using a UV-VIS spectrometer.

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: Using a buffer exchange column (Bio-Rad) they were then moved to coupling buffer (4 M Guanidine HCl, 100 mM Na phosphate pH 6.4, 0.05% Na-azide), protein concentration determined (Bio-Rad microassay) and coupled to beads (Aminolink resin; Pierce) to form an affinity column. .. The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: White plaques of recombinant viruses were expanded and screened by PCR using TRAIL-specific primers. .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Sonication:

    Article Title: Structural Basis for Iron-mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases ‡
    Article Snippet: Cell pellets from 2 L of culture were lysed by sonication on ice in 20 mM Tris, 500 mM NaCl, 10 mM imidazole, pH 8.0. .. The eluted samples were buffer exchanged into 10 mM Tris, 50 mM NaCl, pH 8.0 using an Econo-Pac 10DG desalting column (Bio-Rad).

    Injection:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: 1 Gigabequerel (GBq) of the ASU-synthesized [18 F]SFB was injected into the semi-preparative HPLC column and the purification was initiated with an acetonitrile:water elution gradient of 15:85 reaching 50:50 (v/v) in 12 min. .. 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13.

    Recombinant:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: Paragraph title: Generation of a Recombinant TRAIL Virus. ... TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Article Title: The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis
    Article Snippet: Metal use assays 140 µl of 400 µM recombinant wildtype Tde1415 was diluted to a volume of 3.0 ml with 50 mM EDTA, 50 mM Tris–HCl, pH 8.0 buffer. .. The protein solution was applied to an Econo-Pac 10DG desalting column (Bio-Rad) and exchanged into 50 mM EDTA, 50 mM Tris–Cl, pH 8.0 buffer.

    Molecular Weight:

    Article Title: Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
    Article Snippet: In the samples derived from the flask production trials, all buffer exchanges were conducted using the Spin-X UF concentrators (Corning, Corning, NY, USA) with a 5 kDa molecular weight cut-off PES (polyethersulfone) membrane. .. In the case of the bioreactor production trials, all buffer exchanges were conducted with the use of Econo-Pac 10DG columns (Bio Rad, Hercules, CA, USA) as per the manufacturer’s instructions.

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8. .. The protein was concentrated by Amicon centrifugal filters (molecular weight cut-off 10,000, Millipore) to a final concentration of ∼15 mg/ml, as determined by the Bradford method using bovine serum albumin as a standard (Sigma).

    Radioactivity:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13. .. 350 μL elution fractions were collected from the desalting column and the associated radioactivity was measured on an Atomlab 400 dose calibrator (Biodex).

    Article Title: Efficient one-step radiolabeling of monoclonal antibodies to high specific activity with Actinium-225 for alpha-particle radioimmunotherapy of cancer
    Article Snippet: Paragraph title: Radiolabeling Procedures ... The reaction was then quenched with 50 mM DTPA (20 μl) and purified using an Econo-Pac 10DG desalting column (Bio-Rad) that had been equilibrated previously with 1% Human Serum Albumin (HSA).

    Mutagenesis:

    Article Title: Disruption of the Proton Relay Network in the Class 2 Dihydroorotate Dehydrogenase from Escherichia coli †
    Article Snippet: All mutant enzymes were overexpressed from the pAG-1 plasmid in SØ6645 E. coli cells ( ). .. Before use, enzymes were exchanged into the appropriate buffer using Econo-Pac 10DG disposable desalting columns (Bio-Rad).

    Isolation:

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: As the overexpressed proteins are isolated in the apo-form, IsdG and IsdI at 10 mg/ml were reconstituted with metalloporphyrins ( ). .. DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8.

    Labeling:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: Paragraph title: SFB labeling of scFv-B43.13 ... 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13.

    Purification:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: Paragraph title: Protein overproduction and purification ... LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min).

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad). .. In vitro, TRAIL-Ad induced apoptosis of several tumor cell lines including Rat-1 cells and 293 cells; addition of sDR5 to the culture prevented TRAIL-Ad–induced apoptosis (Göke, A., and Y. Chen, unpublished results).

    Article Title: Disruption of the Proton Relay Network in the Class 2 Dihydroorotate Dehydrogenase from Escherichia coli †
    Article Snippet: Paragraph title: Overexpression and Purification ... Before use, enzymes were exchanged into the appropriate buffer using Econo-Pac 10DG disposable desalting columns (Bio-Rad).

    Article Title: Structural Basis for Iron-mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases ‡
    Article Snippet: Paragraph title: Cloning, Overexpression and Purification ... The eluted samples were buffer exchanged into 10 mM Tris, 50 mM NaCl, pH 8.0 using an Econo-Pac 10DG desalting column (Bio-Rad).

    Article Title: A Cytosolic Serine Endopeptidase from Trypanosoma cruzi Is Required for the Generation of Ca2+ Signaling in Mammalian Cells
    Article Snippet: Paragraph title: Peptidase Purification and Amino Acid Sequencing ... Precipitated protein was recovered by centrifugation at 15,000 g for 30 min, and pellets were resuspended in PBS2+ containing 20 μM E-64, filtered through a 0.22-μm filter unit ( Millipore Products Division, Bedford, MA) and desalted on an Econo-Pac® 10DG column (BioRad Laboratories, Hercules, CA) equilibrated in PBS2+ .

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: .. 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13. .. 350 μL elution fractions were collected from the desalting column and the associated radioactivity was measured on an Atomlab 400 dose calibrator (Biodex).

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: Protein Expression and Crystallization —IsdG-N7A and IsdI were expressed in E. coli BL21 (DE3) and purified as reported previously ( ). .. DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8.

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: Paragraph title: Purification of RAD51 and RAD51 paralogues, and antiserum generation ... The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Article Title: Efficient one-step radiolabeling of monoclonal antibodies to high specific activity with Actinium-225 for alpha-particle radioimmunotherapy of cancer
    Article Snippet: .. The reaction was then quenched with 50 mM DTPA (20 μl) and purified using an Econo-Pac 10DG desalting column (Bio-Rad) that had been equilibrated previously with 1% Human Serum Albumin (HSA). ..

    Sequencing:

    Article Title: A Cytosolic Serine Endopeptidase from Trypanosoma cruzi Is Required for the Generation of Ca2+ Signaling in Mammalian Cells
    Article Snippet: Paragraph title: Peptidase Purification and Amino Acid Sequencing ... Precipitated protein was recovered by centrifugation at 15,000 g for 30 min, and pellets were resuspended in PBS2+ containing 20 μM E-64, filtered through a 0.22-μm filter unit ( Millipore Products Division, Bedford, MA) and desalted on an Econo-Pac® 10DG column (BioRad Laboratories, Hercules, CA) equilibrated in PBS2+ .

    Affinity Column:

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: Using a buffer exchange column (Bio-Rad) they were then moved to coupling buffer (4 M Guanidine HCl, 100 mM Na phosphate pH 6.4, 0.05% Na-azide), protein concentration determined (Bio-Rad microassay) and coupled to beads (Aminolink resin; Pierce) to form an affinity column. .. The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Thin Layer Chromatography:

    Article Title: Synthesis and pre-clinical evaluation of an 18F-labeled single-chain antibody fragment for PET imaging of epithelial ovarian cancer
    Article Snippet: 18 F-labeled scFv was further purified by passing the reaction mixture through an Econo-Pac® 10DG desalting column (Bio-Rad, 732-2010) pre-equilibrated with 0.9% saline (Becton Dickinson, 306572), which was also used as the eluent for purification of the 18 F-labeled scFv-B43.13. .. Radiochemical yields and purity were determined by thin layer chromatography on TLC Silica Gel 60 F254 (Merck KGaA, Germany) using a 5:1 (v/v) mixture of ethyl acetate: hexane as the eluent.

    Affinity Purification:

    Article Title: Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
    Article Snippet: Paragraph title: 2.5. Nickel Affinity Purification of Hydrophobins ... In the case of the bioreactor production trials, all buffer exchanges were conducted with the use of Econo-Pac 10DG columns (Bio Rad, Hercules, CA, USA) as per the manufacturer’s instructions.

    Article Title: Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Article Snippet: For affinity purification, the resin was washed with 5 ml 0.05% sodium azide/PBS (Sigma) and antiserum added. .. The buffer of fractions to be used was exchanged into PBS using a Bio-Rad Econo-Pac 10DG column, following manufacturer's instructions.

    Plasmid Preparation:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: After amplification of the pCRII-TOPO-TRAIL vector DNA, the TRAIL gene was cut out with NotI and inserted into the NotI site of the vector pAdtet to create pAdtet-TRAIL. .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Article Title: Disruption of the Proton Relay Network in the Class 2 Dihydroorotate Dehydrogenase from Escherichia coli †
    Article Snippet: All mutant enzymes were overexpressed from the pAG-1 plasmid in SØ6645 E. coli cells ( ). .. Before use, enzymes were exchanged into the appropriate buffer using Econo-Pac 10DG disposable desalting columns (Bio-Rad).

    Binding Assay:

    Article Title: Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
    Article Snippet: Then, the binding buffer was changed to the elution buffer (20 mM, 500 mM NaCl, 500 mM imidazole pH 7.4) and 5 mL fractions were collected for SDS-PAGE analysis. .. In the case of the bioreactor production trials, all buffer exchanges were conducted with the use of Econo-Pac 10DG columns (Bio Rad, Hercules, CA, USA) as per the manufacturer’s instructions.

    In Vitro:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad). .. In vitro, TRAIL-Ad induced apoptosis of several tumor cell lines including Rat-1 cells and 293 cells; addition of sDR5 to the culture prevented TRAIL-Ad–induced apoptosis (Göke, A., and Y. Chen, unpublished results).

    Nuclear Magnetic Resonance:

    Article Title: The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis
    Article Snippet: The protein solution was applied to an Econo-Pac 10DG desalting column (Bio-Rad) and exchanged into 50 mM EDTA, 50 mM Tris–Cl, pH 8.0 buffer. .. The de-metalated protein was again concentrated to 2.0 ml by ultracentrifugation and stored at 4 o C. PntC activity was monitored by 31 P NMR after 2 h incubation at room temperature in 50 mM Tris–Cl pH 8.0 containing 7.0 mM CTP, 3 mM AEP, 5.5 µM Tde1415, and 7.0 mM of divalent metal (MgCl2 , CaCl2 , or ZnCl2 ).

    Produced:

    Article Title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Is an Inhibitor of Autoimmune Inflammation and Cell Cycle Progression
    Article Snippet: The recombinant virus was produced by cotransfecting 293 cells with pAdtet-TRAIL and ClaI-digested Ad5 genomic DNA. .. TRAIL-Ad was then propagated, purified through a cesium chloride gradient, and desalted on an Econo-Pac 10DG column (Bio-Rad).

    Concentration Assay:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: Following the cell disruption, MgCl2 (final concentration 10 mM) and 1 mg/ml DNaseI was added and the lysate was incubated for 1 hour at 30°C. .. LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min).

    Article Title: A Cytosolic Serine Endopeptidase from Trypanosoma cruzi Is Required for the Generation of Ca2+ Signaling in Mammalian Cells
    Article Snippet: Briefly, epimastigotes, grown and harvested as described above, were resuspended in PBS2+ at a concentration of 5 × 108 /ml, heated to 56°C for 5 min, and then immediately frozen at −80°C. .. Precipitated protein was recovered by centrifugation at 15,000 g for 30 min, and pellets were resuspended in PBS2+ containing 20 μM E-64, filtered through a 0.22-μm filter unit ( Millipore Products Division, Bedford, MA) and desalted on an Econo-Pac® 10DG column (BioRad Laboratories, Hercules, CA) equilibrated in PBS2+ .

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8. .. The protein was concentrated by Amicon centrifugal filters (molecular weight cut-off 10,000, Millipore) to a final concentration of ∼15 mg/ml, as determined by the Bradford method using bovine serum albumin as a standard (Sigma).

    Diffusion-based Assay:

    Article Title: Ruffling of Metalloporphyrins Bound to IsdG and IsdI, Two Heme-degrading Enzymes in Staphylococcus aureus * * S⃞
    Article Snippet: DMSO and excess metalloporphyrins were removed using an Econo-Pac 10DG desalting column (Bio-Rad) equilibrated with 200 m m NaCl and 20 m m Tris-HCl, pH 8. .. Deeply colored crystals were obtained by mixing equal volumes of protein solution with crystallization buffers in a hanging drop vapor diffusion setup.

    Strep-tag:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: The supernatant was incubated with 6 mL Strep-tag Tactin slurry (50% Strep-tag Tactin in 50 mM potassium phosphate buffer, pH 8, 150 mM NaCl, 10% glycerol), for one hour on a rotary shaker at 4°C. .. LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min).

    Staining:

    Article Title: Binding of the Lactococcal Drug Dependent Transcriptional Regulator LmrR to Its Ligands and Responsive Promoter Regions
    Article Snippet: LmrR-Strep containing fractions were desalted to 50 mM PB, pH 8 using a Econo-PAC 10DG column (Bio-Rad) and applied to a 5 mL Heparin column (pre-equilibrated with 50 mM PB, pH 8), LmrR was eluted using a gradient of 2 M NaCl (0 to 100%, 10 min). .. Fractions were analyzed on a 12% SDS polyacrylamide gel, stained with Coomassie Brilliant Blue, and the protein concentration and purity was assessed using a UV-VIS spectrometer.

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    Bio-Rad econo pac 10 dg disposable chromatography columns
    Econo Pac 10 Dg Disposable Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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