eco32i  (Thermo Fisher)


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    Name:
    Eco32I EcoRV
    Description:
    5 G A T ↓A T C 3 3 C T A ↑T A G 5 Thermo Scientific Eco32I EcoRV restriction enzyme recognizes GAT ATC sites and cuts best at 37°C in R buffer Isoschizomers EcoRV See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    ER0303
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Cloning|Restriction Enzyme Cloning
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    Structured Review

    Thermo Fisher eco32i
    Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, <t>Eco32I,</t> HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler
    5 G A T ↓A T C 3 3 C T A ↑T A G 5 Thermo Scientific Eco32I EcoRV restriction enzyme recognizes GAT ATC sites and cuts best at 37°C in R buffer Isoschizomers EcoRV See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/eco32i/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eco32i - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "Molecular Characterization of a Novel Temperate Sinorhizobium Bacteriophage, ФLM21, Encoding DNA Methyltransferase with CcrM-Like Specificity"

    Article Title: Molecular Characterization of a Novel Temperate Sinorhizobium Bacteriophage, ФLM21, Encoding DNA Methyltransferase with CcrM-Like Specificity

    Journal: Journal of Virology

    doi: 10.1128/JVI.01875-14

    Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, Eco32I, HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler
    Figure Legend Snippet: Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, Eco32I, HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler

    Techniques Used: In Vitro, Methylation, Generated

    2) Product Images from "Two Inducible Prophages of an Antarctic Pseudomonas sp. ANT_H14 Use the Same Capsid for Packaging Their Genomes – Characterization of a Novel Phage Helper-Satellite System"

    Article Title: Two Inducible Prophages of an Antarctic Pseudomonas sp. ANT_H14 Use the Same Capsid for Packaging Their Genomes – Characterization of a Novel Phage Helper-Satellite System

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0158889

    Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), Eco32I (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.
    Figure Legend Snippet: Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), Eco32I (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.

    Techniques Used: Purification, Marker

    Related Articles

    Electrophoresis:

    Article Title: Targeted Chromosomal Insertion of Large DNA into the Human Genome by a Fiber-Modified High-Capacity Adenovirus-Based Vector System
    Article Snippet: Chromosomal DNA extraction and analysis The purification of chromosomal DNA was carried essentially as previously described except for the use of a different lysis buffer consisting of 100 mM Tris-HCl (pH 8.5), 5 mM EDTA (pH 8.0), 0.2% SDS and 200 mM NaCl. .. After restriction enzyme digestion with Eco32I (Fermentas) and electrophoresis through 0.8% agarose gels in 1× Tris-acetate-EDTA buffer, the DNA was transferred by capillary action onto Amersham Hybond-XL membranes. .. These membranes were subsequently incubated with blocking solution, exposed to the DNA probes specified below and repeatedly washed using a standard Southern hybridization procedure.

    Polymerase Chain Reaction:

    Article Title: Polymorphisms in the Mn-SOD and EC-SOD Genes and Their Relationship to Diabetic Neuropathy in Type 1 Diabetes Mellitus
    Article Snippet: For the Ile58Thr polymorphism, the amplified fragment was digested with Eco 32I (both enzymes from Fermentas, Lithuania). .. For digestion, 17 μl of PCR product, 2 μl of buffer O+ /Tango™ (BshTI) or R+ /Tango™ (Eco32I) (all buffers from Fermentas, Lithuania), and 1 μl of restriction endonuclease (1 unit/μl) were mixed and incubated for 12 h at 37°C. .. Digested DNA products were separated by electrophoresis in a 3% agarose gel with ethidium bromide or in an 8% polyacrylamide gel stained with silver [ ].

    Incubation:

    Article Title: Polymorphisms in the Mn-SOD and EC-SOD Genes and Their Relationship to Diabetic Neuropathy in Type 1 Diabetes Mellitus
    Article Snippet: For the Ile58Thr polymorphism, the amplified fragment was digested with Eco 32I (both enzymes from Fermentas, Lithuania). .. For digestion, 17 μl of PCR product, 2 μl of buffer O+ /Tango™ (BshTI) or R+ /Tango™ (Eco32I) (all buffers from Fermentas, Lithuania), and 1 μl of restriction endonuclease (1 unit/μl) were mixed and incubated for 12 h at 37°C. .. Digested DNA products were separated by electrophoresis in a 3% agarose gel with ethidium bromide or in an 8% polyacrylamide gel stained with silver [ ].

    Construct:

    Article Title: Precise and broad scope genome editing based on high-specificity Cas9 nickases
    Article Snippet: .. Constructs AW01_pU.CAG.Cas9-eSp(1.1).rBGpA ( ) and BB36_pCAG.Cas9eSp(1.1)-D10A.bGHpA were digested with BshTI and Eco32I. .. Subsequently, the 7378-bp backbone fragment from AW01_pU.CAG.Cas9-eSp(1.1).rBGpA ( ) and the 1982-bp insert fragment from BB36_pCAG.Cas9eSp(1.1)-D10A.bGHpA were extracted from agarose gel and ligated together, leading to the generation of construct AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS encoding eSpCas9(1.1)D10A .

    Article Title: Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer
    Article Snippet: ) canonical notation sequence # –1. .. For generating the mutants, the PLEX307 plasmid containing the SMARCA4 gene (V5-BRG1) was co-digested with FspAI and Eco32I (Thermofisher) and the mutations were inserted by ligating synthetic constructs (gblocks by IDT DNA Technologies) containing the desire mutations using the Gibson assembly method ( ). ..

    Plasmid Preparation:

    Article Title: Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer
    Article Snippet: ) canonical notation sequence # –1. .. For generating the mutants, the PLEX307 plasmid containing the SMARCA4 gene (V5-BRG1) was co-digested with FspAI and Eco32I (Thermofisher) and the mutations were inserted by ligating synthetic constructs (gblocks by IDT DNA Technologies) containing the desire mutations using the Gibson assembly method ( ). ..

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  • 93
    Thermo Fisher eco32i
    Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, <t>Eco32I,</t> HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler
    Eco32i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eco32i/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eco32i - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher fastdigest eco32i
    Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, <t>Eco32I,</t> HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler
    Fastdigest Eco32i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest eco32i/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fastdigest eco32i - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, Eco32I, HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler

    Journal: Journal of Virology

    Article Title: Molecular Characterization of a Novel Temperate Sinorhizobium Bacteriophage, ФLM21, Encoding DNA Methyltransferase with CcrM-Like Specificity

    doi: 10.1128/JVI.01875-14

    Figure Lengend Snippet: Comparison of restriction patterns of λ dam − dcm − DNA (controls, lanes C) and Orf27 in vitro -methylated λ DNA (lanes Orf27) generated with MboI, DpnI, Eco32I, HinfI, and MspI REases. ND, undigested λ DNA. M, GeneRuler

    Article Snippet: The test for the presence of cohesive ends of the phage genome was performed as previously described , using the restriction enzymes HindIII, XhoI, Eco32I, SalI, and SmaI (Thermo Scientific).

    Techniques: In Vitro, Methylation, Generated

    Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), Eco32I (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.

    Journal: PLoS ONE

    Article Title: Two Inducible Prophages of an Antarctic Pseudomonas sp. ANT_H14 Use the Same Capsid for Packaging Their Genomes – Characterization of a Novel Phage Helper-Satellite System

    doi: 10.1371/journal.pone.0158889

    Figure Lengend Snippet: Restriction patterns of DNA extracted from purified virions cleaved with the selected REases. Panel A: HindIII (H), Eco32I (E32), SalI (S) and EcoRI (EI). ND, undigested DNA. M, GeneRuler 100- to 10,000-bp size marker. λ/H, λ DNA cleaved with HindIII. ~14 kb restriction fragment of EcoR32I digested virion DNA is marked with a triangle. Panel B: Arrows indicate restriction fragments assigned to ФAH14a (left) and ФAH14b (right) genomic DNA, obtained by SalI digestion.

    Article Snippet: The test for the presence of cohesive ends of the phage genome was performed as previously described [ ], using the following REases: HindIII, SalI, EcoRI, Eco32I and PstI (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Purification, Marker