Structured Review

Nikon eclipse 80i fluorescence microscope
Dynamics of immune cells infiltrating the tumors of ricolinostat and/or JQ1-treated KP mice Cell suspensions generated from tumor nodules of KP mice that were treated with vehicle, ricolinostat or JQ1 for 5–6 weeks were subjected to FACS analysis to assess proportions of CD45+ T lymphocyte subsets. (A) Proportion of CD4+, CD8+ conventional T cells, or CD4+Foxp3+ Tregs and (B) ratio of CD8 to CD4+Foxp3+ Treg cells within the tumors. Frozen sections of fresh tumor nodules from these treated KP mice were stained for TAMs (CD11c+; red) and T cells (CD3+; green), and counter stained with DAPI (nuclei; blue). (C) Representative immunofluorescent staining of tumor sections from mice treated as indicated. Images were captured on a Nikon Eclipse <t>80i</t> fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Scale bar; 25μm. * indicates p-value ˂ 0.05, ** indicates p-value ˂0.01, *** indicates p-value ˂0.001.
Eclipse 80i Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse 80i fluorescence microscope/product/Nikon
Average 94 stars, based on 631 article reviews
Price from $9.99 to $1999.99
eclipse 80i fluorescence microscope - by Bioz Stars, 2020-08
94/100 stars

Images

1) Product Images from "Synergistic immunostimulatory effects and therapeutic benefit of combined histone deacetylase and bromodomain inhibition in non-small cell lung cancer"

Article Title: Synergistic immunostimulatory effects and therapeutic benefit of combined histone deacetylase and bromodomain inhibition in non-small cell lung cancer

Journal: Cancer discovery

doi: 10.1158/2159-8290.CD-16-1020

Dynamics of immune cells infiltrating the tumors of ricolinostat and/or JQ1-treated KP mice Cell suspensions generated from tumor nodules of KP mice that were treated with vehicle, ricolinostat or JQ1 for 5–6 weeks were subjected to FACS analysis to assess proportions of CD45+ T lymphocyte subsets. (A) Proportion of CD4+, CD8+ conventional T cells, or CD4+Foxp3+ Tregs and (B) ratio of CD8 to CD4+Foxp3+ Treg cells within the tumors. Frozen sections of fresh tumor nodules from these treated KP mice were stained for TAMs (CD11c+; red) and T cells (CD3+; green), and counter stained with DAPI (nuclei; blue). (C) Representative immunofluorescent staining of tumor sections from mice treated as indicated. Images were captured on a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Scale bar; 25μm. * indicates p-value ˂ 0.05, ** indicates p-value ˂0.01, *** indicates p-value ˂0.001.
Figure Legend Snippet: Dynamics of immune cells infiltrating the tumors of ricolinostat and/or JQ1-treated KP mice Cell suspensions generated from tumor nodules of KP mice that were treated with vehicle, ricolinostat or JQ1 for 5–6 weeks were subjected to FACS analysis to assess proportions of CD45+ T lymphocyte subsets. (A) Proportion of CD4+, CD8+ conventional T cells, or CD4+Foxp3+ Tregs and (B) ratio of CD8 to CD4+Foxp3+ Treg cells within the tumors. Frozen sections of fresh tumor nodules from these treated KP mice were stained for TAMs (CD11c+; red) and T cells (CD3+; green), and counter stained with DAPI (nuclei; blue). (C) Representative immunofluorescent staining of tumor sections from mice treated as indicated. Images were captured on a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Scale bar; 25μm. * indicates p-value ˂ 0.05, ** indicates p-value ˂0.01, *** indicates p-value ˂0.001.

Techniques Used: Mouse Assay, Generated, FACS, Staining, Fluorescence, Microscopy, Imaging, Software

2) Product Images from "Effects of 5′-3′ Exonuclease Xrn1 on Cell Size, Proliferation and Division, and mRNA Levels of Periodic Genes in Cryptococcus neoformans"

Article Title: Effects of 5′-3′ Exonuclease Xrn1 on Cell Size, Proliferation and Division, and mRNA Levels of Periodic Genes in Cryptococcus neoformans

Journal: Genes

doi: 10.3390/genes11040430

Measurement of the cell size. ( a ) Yeast strains were cultured in YPD liquid medium at 28 °C or 37 °C, with 180 rpm shaking for 48 h. Cells were harvested and observed with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ( b ). Forty cells were removed randomly from each sample to measure cell size. Data were processed using GraphPad Prism 5 software. Unpaired t -test was used for statistical analysis.
Figure Legend Snippet: Measurement of the cell size. ( a ) Yeast strains were cultured in YPD liquid medium at 28 °C or 37 °C, with 180 rpm shaking for 48 h. Cells were harvested and observed with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ( b ). Forty cells were removed randomly from each sample to measure cell size. Data were processed using GraphPad Prism 5 software. Unpaired t -test was used for statistical analysis.

Techniques Used: Cell Culture, Fluorescence, Microscopy, Software

3) Product Images from "Twist Modulates Breast Cancer Stem Cells by Transcriptional Regulation of CD24 Expression 1"

Article Title: Twist Modulates Breast Cancer Stem Cells by Transcriptional Regulation of CD24 Expression 1

Journal: Neoplasia (New York, N.Y.)

doi:

Efflux studies in MCF-7 and MCF-7/Twist cells. (A) Representative photomicrographs of Hoechst efflux staining in MCF-7/Twist cells compared with parental MCF-7 cells. The cells, after efflux, were photographed using a Nikon Eclipse 80i fluorescence microscope. (B) Histogram showing quantification of fluorescence intensity per cell. Cell fluorescence ( n = 6) in the blue channel was analyzed using dedicated software developed in IDL programming environment that provides an operator-free segmentation of images and determines the average relative fluorescence per cell. Three images were analyzed per sample. (C) Histogram showing expression of drug transporters ABCG2, ABCC1 , and ABCA1 in MCF-7/Twist and parental MCF-7 cells. (D) Histogram showing efflux of Rhodamine 123 stain in MCF-7/Twist and in MCF-7 cells. The amount of Rhodamine 123 dye in the cells was determined by flow cytometry. Results are representative of three separate experiments.
Figure Legend Snippet: Efflux studies in MCF-7 and MCF-7/Twist cells. (A) Representative photomicrographs of Hoechst efflux staining in MCF-7/Twist cells compared with parental MCF-7 cells. The cells, after efflux, were photographed using a Nikon Eclipse 80i fluorescence microscope. (B) Histogram showing quantification of fluorescence intensity per cell. Cell fluorescence ( n = 6) in the blue channel was analyzed using dedicated software developed in IDL programming environment that provides an operator-free segmentation of images and determines the average relative fluorescence per cell. Three images were analyzed per sample. (C) Histogram showing expression of drug transporters ABCG2, ABCC1 , and ABCA1 in MCF-7/Twist and parental MCF-7 cells. (D) Histogram showing efflux of Rhodamine 123 stain in MCF-7/Twist and in MCF-7 cells. The amount of Rhodamine 123 dye in the cells was determined by flow cytometry. Results are representative of three separate experiments.

Techniques Used: Staining, Fluorescence, Microscopy, Software, Expressing, Flow Cytometry, Cytometry

4) Product Images from "Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells"

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Journal: Cell Death Discovery

doi: 10.1038/s41420-018-0053-6

Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p
Figure Legend Snippet: Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p

Techniques Used: Transfection, Staining, Fluorescence, Microscopy, Labeling

5) Product Images from "Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells"

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Journal: Cell Death Discovery

doi: 10.1038/s41420-018-0053-6

Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p
Figure Legend Snippet: Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p

Techniques Used: Transfection, Staining, Fluorescence, Microscopy, Labeling

6) Product Images from "Biodistribution and Molecular Studies on Orally Administered Nanoparticle-AON Complexes Encapsulated with Alginate Aiming at Inducing Dystrophin Rescue in mdx Mice"

Article Title: Biodistribution and Molecular Studies on Orally Administered Nanoparticle-AON Complexes Encapsulated with Alginate Aiming at Inducing Dystrophin Rescue in mdx Mice

Journal: BioMed Research International

doi: 10.1155/2013/527418

Immunofluorescence analysis of intestinal smooth muscle of wild type (WT), untreated ( mdx ) and alginate-ZM2-M23D orally treated (Treated) mdx mice. The sections of small intestine were labeled with antidystrophin antibody. Serial sections of intestine were labeled with a polyclonal antibody for desmin (green). All samples were observed with a Nikon Eclipse 80i fluorescence microscope. Dystrophin (red) is clearly visible in the intestinal smooth muscle of WT mice, absent in untreated mdx , and rescued in treated mdx mice. (Scale bar = 50 μ m).
Figure Legend Snippet: Immunofluorescence analysis of intestinal smooth muscle of wild type (WT), untreated ( mdx ) and alginate-ZM2-M23D orally treated (Treated) mdx mice. The sections of small intestine were labeled with antidystrophin antibody. Serial sections of intestine were labeled with a polyclonal antibody for desmin (green). All samples were observed with a Nikon Eclipse 80i fluorescence microscope. Dystrophin (red) is clearly visible in the intestinal smooth muscle of WT mice, absent in untreated mdx , and rescued in treated mdx mice. (Scale bar = 50 μ m).

Techniques Used: Immunofluorescence, Mouse Assay, Labeling, Fluorescence, Microscopy

7) Product Images from "Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells"

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Journal: Cell Death Discovery

doi: 10.1038/s41420-018-0053-6

Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p
Figure Legend Snippet: Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p

Techniques Used: Transfection, Staining, Fluorescence, Microscopy, Labeling

8) Product Images from "Activation of Type I and III Interferon Signalling Pathways Occurs in Lung Epithelial Cells Infected with Low Pathogenic Avian Influenza Viruses"

Article Title: Activation of Type I and III Interferon Signalling Pathways Occurs in Lung Epithelial Cells Infected with Low Pathogenic Avian Influenza Viruses

Journal: PLoS ONE

doi: 10.1371/journal.pone.0033732

Influenza virus induced changes in STAT1 signalling during virus infection. (A) A549 cells were infected with either the H1N1/WSN, H9N2, H5N2/F118 or H5N3 viruses or (B) the pH1N1/471, pH1N1/478 or pH1N1/527 virus using an MOI = 4. Then cells were harvested at between 0.2 and 18 hpi in SDS-PAGE boiling mix as described in methods . The proteins were transferred on to PVDF membranes by western blotting, and the membranes probed with the appropriate primary and secondary antibodies. The phosphorylated STAT-1 (pSTAT1) and total STAT-1(STAT1) are shown. (A) β-catenin or (B) β-actin provides a loading control. (C) A549 cells were either mock-infected or were infected with the H1N1/WSN, H5N2, H9N2, H5N3, pH1N1/471 or pH1N1/527 viruses using an MOI = 4. At 16 hpi the cells were labelled using anti-MX and goat anti-mouse conjugated to Alexa555. The stained cells were visualised using a Nikon Eclipse 80i Microscope at ×20 magnification using appropriate machine settings.
Figure Legend Snippet: Influenza virus induced changes in STAT1 signalling during virus infection. (A) A549 cells were infected with either the H1N1/WSN, H9N2, H5N2/F118 or H5N3 viruses or (B) the pH1N1/471, pH1N1/478 or pH1N1/527 virus using an MOI = 4. Then cells were harvested at between 0.2 and 18 hpi in SDS-PAGE boiling mix as described in methods . The proteins were transferred on to PVDF membranes by western blotting, and the membranes probed with the appropriate primary and secondary antibodies. The phosphorylated STAT-1 (pSTAT1) and total STAT-1(STAT1) are shown. (A) β-catenin or (B) β-actin provides a loading control. (C) A549 cells were either mock-infected or were infected with the H1N1/WSN, H5N2, H9N2, H5N3, pH1N1/471 or pH1N1/527 viruses using an MOI = 4. At 16 hpi the cells were labelled using anti-MX and goat anti-mouse conjugated to Alexa555. The stained cells were visualised using a Nikon Eclipse 80i Microscope at ×20 magnification using appropriate machine settings.

Techniques Used: Infection, SDS Page, Western Blot, Staining, Microscopy

9) Product Images from "Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis"

Article Title: Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis

Journal: Journal of Breast Cancer

doi: 10.4048/jbc.2016.19.4.358

Release of cytochrome-c (cyt c) from alkyl cinnamate treated MDAMB-231 breast cancer cells. MDAMB-231 breast cancer cells were treated with alkyl cinnamates (IC 50 ) in serum-free media for 24 hours. After treatment, cells were immune-stained with anti-cyt c antibodies and location of mitochondria in MDAMB-231 cells is determined by loading the cells with MitoTracker (BD-Biosciences; 200 nM for 45 minutes) as given in material and methods. Labelled cells were observed in a fluorescent microscope 80i (Nikon). In each panel, bright field, cyt c (green), MitoTracker Red (red) is given. High resolution fluorescence microscopy detected release of cyt c from DM2-3, DM2-4, DM2-5 and curcumin treated cells. Untreated cells seem healthy with the cyt c signal overlapping with MitoTracker signal.
Figure Legend Snippet: Release of cytochrome-c (cyt c) from alkyl cinnamate treated MDAMB-231 breast cancer cells. MDAMB-231 breast cancer cells were treated with alkyl cinnamates (IC 50 ) in serum-free media for 24 hours. After treatment, cells were immune-stained with anti-cyt c antibodies and location of mitochondria in MDAMB-231 cells is determined by loading the cells with MitoTracker (BD-Biosciences; 200 nM for 45 minutes) as given in material and methods. Labelled cells were observed in a fluorescent microscope 80i (Nikon). In each panel, bright field, cyt c (green), MitoTracker Red (red) is given. High resolution fluorescence microscopy detected release of cyt c from DM2-3, DM2-4, DM2-5 and curcumin treated cells. Untreated cells seem healthy with the cyt c signal overlapping with MitoTracker signal.

Techniques Used: Staining, Microscopy, Fluorescence

10) Product Images from "Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells"

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Journal: Cell Death Discovery

doi: 10.1038/s41420-018-0053-6

Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p
Figure Legend Snippet: Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p

Techniques Used: Transfection, Staining, Fluorescence, Microscopy, Labeling

11) Product Images from "Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis"

Article Title: Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis

Journal: Journal of Breast Cancer

doi: 10.4048/jbc.2016.19.4.358

Release of cytochrome-c (cyt c) from alkyl cinnamate treated MDAMB-231 breast cancer cells. MDAMB-231 breast cancer cells were treated with alkyl cinnamates (IC 50 ) in serum-free media for 24 hours. After treatment, cells were immune-stained with anti-cyt c antibodies and location of mitochondria in MDAMB-231 cells is determined by loading the cells with MitoTracker (BD-Biosciences; 200 nM for 45 minutes) as given in material and methods. Labelled cells were observed in a fluorescent microscope 80i (Nikon). In each panel, bright field, cyt c (green), MitoTracker Red (red) is given. High resolution fluorescence microscopy detected release of cyt c from DM2-3, DM2-4, DM2-5 and curcumin treated cells. Untreated cells seem healthy with the cyt c signal overlapping with MitoTracker signal.
Figure Legend Snippet: Release of cytochrome-c (cyt c) from alkyl cinnamate treated MDAMB-231 breast cancer cells. MDAMB-231 breast cancer cells were treated with alkyl cinnamates (IC 50 ) in serum-free media for 24 hours. After treatment, cells were immune-stained with anti-cyt c antibodies and location of mitochondria in MDAMB-231 cells is determined by loading the cells with MitoTracker (BD-Biosciences; 200 nM for 45 minutes) as given in material and methods. Labelled cells were observed in a fluorescent microscope 80i (Nikon). In each panel, bright field, cyt c (green), MitoTracker Red (red) is given. High resolution fluorescence microscopy detected release of cyt c from DM2-3, DM2-4, DM2-5 and curcumin treated cells. Untreated cells seem healthy with the cyt c signal overlapping with MitoTracker signal.

Techniques Used: Staining, Microscopy, Fluorescence

12) Product Images from "Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells"

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Journal: Cell Death Discovery

doi: 10.1038/s41420-018-0053-6

Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p
Figure Legend Snippet: Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p

Techniques Used: Transfection, Staining, Fluorescence, Microscopy, Labeling

13) Product Images from "Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells"

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells

Journal: Cell Death Discovery

doi: 10.1038/s41420-018-0053-6

Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p
Figure Legend Snippet: Effect of siHSP90 and siLamin A/C on self-aggregation formation of DPCs. a The siControl-, siHSP90-, and siLamin A/C-transfected DPCs were seeded at a high-density of 3 × 10 5 cells in each of 6-well plate and maintained for 72 h to allow self-aggregation formation. Thereafter, the cell nuclei were stained by Hoechst dye (in blue) and the cells were examined under an ECLIPSE 80i fluorescence microscope (Nikon) (Original magnification = ×100). The DPCs’ cell clumps indicating self-aggregation formation are labeled with white dotted circle. b Number of the cell aggregates in each condition was counted from at least 25 low-power fields (LPF) per well. Each bar represents mean ± SEM of the data obtained from three independent experiments. * p

Techniques Used: Transfection, Staining, Fluorescence, Microscopy, Labeling

Related Articles

Staining:

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. The stained cells were then examined under an ECLIPSE 80i fluorescence microscope (Nikon) and the number of DPCs aggregates in each condition was counted from at least 25 fields per well. ..

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. For single staining, the cells were incubated with mouse monoclonal anti-vimentin antibody or mouse monoclonal anti-fibronectin (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. For co-staining, the cells were incubated with both rabbit polyclonal anti-HSP90 antibody and mouse monoclonal anti-lamin A/C (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. The cells were rinsed with PBS three times and further incubated with Alexa Flour® 555-conjugated or Alexa Fluor® 488-conjugated secondary antibody (both were from Invitrogen-Molecular Probes, Burlinton, ON, Canada; and were diluted 1:2000 in 1%BSA/PBS) mixed with 0.1 µg/ml Hoechst dye (DNA staining for nuclear localization) (Sigma, St. Louis, MO) at 37 °C for 1 h. Thereafter, the stained cells were washed with PBS and mounted with 50% glycerol/PBS for subsequent examination using an ECLIPSE 80i fluorescence microscope (Nikon). .. Mean fluorescence intensity representing protein level was analyzed from 10 random high-power fields (at least 100 cells) in each well using NIS-Elements D V.4.11 (Nikon) , .

Article Title: Activation of Type I and III Interferon Signalling Pathways Occurs in Lung Epithelial Cells Infected with Low Pathogenic Avian Influenza Viruses
Article Snippet: .. The stained cells were mounted on slides using Dakocytomation (Dako, USA) and visualized either using a Nikon eclipse 80i fluorescence microscope, or a Zeiss Axioplan 2 LSM510 confocal microscope using appropriate machine settings. .. Confocal microscopy images were processed using LSM510 software.

Incubation:

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. For single staining, the cells were incubated with mouse monoclonal anti-vimentin antibody or mouse monoclonal anti-fibronectin (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. For co-staining, the cells were incubated with both rabbit polyclonal anti-HSP90 antibody and mouse monoclonal anti-lamin A/C (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. The cells were rinsed with PBS three times and further incubated with Alexa Flour® 555-conjugated or Alexa Fluor® 488-conjugated secondary antibody (both were from Invitrogen-Molecular Probes, Burlinton, ON, Canada; and were diluted 1:2000 in 1%BSA/PBS) mixed with 0.1 µg/ml Hoechst dye (DNA staining for nuclear localization) (Sigma, St. Louis, MO) at 37 °C for 1 h. Thereafter, the stained cells were washed with PBS and mounted with 50% glycerol/PBS for subsequent examination using an ECLIPSE 80i fluorescence microscope (Nikon). .. Mean fluorescence intensity representing protein level was analyzed from 10 random high-power fields (at least 100 cells) in each well using NIS-Elements D V.4.11 (Nikon) , .

Fluorescence:

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. The stained cells were then examined under an ECLIPSE 80i fluorescence microscope (Nikon) and the number of DPCs aggregates in each condition was counted from at least 25 fields per well. ..

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. For single staining, the cells were incubated with mouse monoclonal anti-vimentin antibody or mouse monoclonal anti-fibronectin (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. For co-staining, the cells were incubated with both rabbit polyclonal anti-HSP90 antibody and mouse monoclonal anti-lamin A/C (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. The cells were rinsed with PBS three times and further incubated with Alexa Flour® 555-conjugated or Alexa Fluor® 488-conjugated secondary antibody (both were from Invitrogen-Molecular Probes, Burlinton, ON, Canada; and were diluted 1:2000 in 1%BSA/PBS) mixed with 0.1 µg/ml Hoechst dye (DNA staining for nuclear localization) (Sigma, St. Louis, MO) at 37 °C for 1 h. Thereafter, the stained cells were washed with PBS and mounted with 50% glycerol/PBS for subsequent examination using an ECLIPSE 80i fluorescence microscope (Nikon). .. Mean fluorescence intensity representing protein level was analyzed from 10 random high-power fields (at least 100 cells) in each well using NIS-Elements D V.4.11 (Nikon) , .

Article Title: Synergistic immunostimulatory effects and therapeutic benefit of combined histone deacetylase and bromodomain inhibition in non-small cell lung cancer
Article Snippet: .. Sections were imaged using a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera (Roper Scientific). .. NIS elements Imaging software was used to create merged images.

Article Title: Effects of 5′-3′ Exonuclease Xrn1 on Cell Size, Proliferation and Division, and mRNA Levels of Periodic Genes in Cryptococcus neoformans
Article Snippet: .. Determining the Cell Size, Growth Curves and Budding Rate For cell size measurement, yeast strains were cultured in the YPD liquid medium at 28 °C or 37 °C, with shaking speed of 180 rpm for 48 h. Cells were harvested and the cell size was measured with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ..

Article Title: Activation of Type I and III Interferon Signalling Pathways Occurs in Lung Epithelial Cells Infected with Low Pathogenic Avian Influenza Viruses
Article Snippet: .. The stained cells were mounted on slides using Dakocytomation (Dako, USA) and visualized either using a Nikon eclipse 80i fluorescence microscope, or a Zeiss Axioplan 2 LSM510 confocal microscope using appropriate machine settings. .. Confocal microscopy images were processed using LSM510 software.

Article Title: Biodistribution and Molecular Studies on Orally Administered Nanoparticle-AON Complexes Encapsulated with Alginate Aiming at Inducing Dystrophin Rescue in mdx Mice
Article Snippet: .. All images were observed with a Nikon Eclipse 80i fluorescence microscope (Nikon Instruments, Firenze, Italy) connected to at a high-resolution CCD camera (Nikon Instruments, Firenze, Italy) at 20x magnification. .. Dystrophin Analysis by Western Blotting Western blot analysis was performed as previously described [ ].

Article Title: Twist Modulates Breast Cancer Stem Cells by Transcriptional Regulation of CD24 Expression 1
Article Snippet: .. Sections were mounted using Permount (Thermo-Fisher Scientific, Waltham, MA) and photographed on a Nikon Eclipse 80i fluorescence microscope using a CoolSnap ES camera (Nikon Instruments). .. Recent studies have demonstrated that the cell surface expression pattern of CD44 and CD24 constitutes a major identifying marker of breast cancer stem cells.

Microscopy:

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. The stained cells were then examined under an ECLIPSE 80i fluorescence microscope (Nikon) and the number of DPCs aggregates in each condition was counted from at least 25 fields per well. ..

Article Title: Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Article Snippet: .. For single staining, the cells were incubated with mouse monoclonal anti-vimentin antibody or mouse monoclonal anti-fibronectin (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. For co-staining, the cells were incubated with both rabbit polyclonal anti-HSP90 antibody and mouse monoclonal anti-lamin A/C (both were from Santa Cruz Biotechnology and diluted 1:50 in 1% BSA/PBS) at 37 °C for 1 h. The cells were rinsed with PBS three times and further incubated with Alexa Flour® 555-conjugated or Alexa Fluor® 488-conjugated secondary antibody (both were from Invitrogen-Molecular Probes, Burlinton, ON, Canada; and were diluted 1:2000 in 1%BSA/PBS) mixed with 0.1 µg/ml Hoechst dye (DNA staining for nuclear localization) (Sigma, St. Louis, MO) at 37 °C for 1 h. Thereafter, the stained cells were washed with PBS and mounted with 50% glycerol/PBS for subsequent examination using an ECLIPSE 80i fluorescence microscope (Nikon). .. Mean fluorescence intensity representing protein level was analyzed from 10 random high-power fields (at least 100 cells) in each well using NIS-Elements D V.4.11 (Nikon) , .

Article Title: Synergistic immunostimulatory effects and therapeutic benefit of combined histone deacetylase and bromodomain inhibition in non-small cell lung cancer
Article Snippet: .. Sections were imaged using a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera (Roper Scientific). .. NIS elements Imaging software was used to create merged images.

Article Title: Effects of 5′-3′ Exonuclease Xrn1 on Cell Size, Proliferation and Division, and mRNA Levels of Periodic Genes in Cryptococcus neoformans
Article Snippet: .. Determining the Cell Size, Growth Curves and Budding Rate For cell size measurement, yeast strains were cultured in the YPD liquid medium at 28 °C or 37 °C, with shaking speed of 180 rpm for 48 h. Cells were harvested and the cell size was measured with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ..

Article Title: Activation of Type I and III Interferon Signalling Pathways Occurs in Lung Epithelial Cells Infected with Low Pathogenic Avian Influenza Viruses
Article Snippet: .. The stained cells were mounted on slides using Dakocytomation (Dako, USA) and visualized either using a Nikon eclipse 80i fluorescence microscope, or a Zeiss Axioplan 2 LSM510 confocal microscope using appropriate machine settings. .. Confocal microscopy images were processed using LSM510 software.

Article Title: Biodistribution and Molecular Studies on Orally Administered Nanoparticle-AON Complexes Encapsulated with Alginate Aiming at Inducing Dystrophin Rescue in mdx Mice
Article Snippet: .. All images were observed with a Nikon Eclipse 80i fluorescence microscope (Nikon Instruments, Firenze, Italy) connected to at a high-resolution CCD camera (Nikon Instruments, Firenze, Italy) at 20x magnification. .. Dystrophin Analysis by Western Blotting Western blot analysis was performed as previously described [ ].

Article Title: Twist Modulates Breast Cancer Stem Cells by Transcriptional Regulation of CD24 Expression 1
Article Snippet: .. Sections were mounted using Permount (Thermo-Fisher Scientific, Waltham, MA) and photographed on a Nikon Eclipse 80i fluorescence microscope using a CoolSnap ES camera (Nikon Instruments). .. Recent studies have demonstrated that the cell surface expression pattern of CD44 and CD24 constitutes a major identifying marker of breast cancer stem cells.

Cell Culture:

Article Title: Effects of 5′-3′ Exonuclease Xrn1 on Cell Size, Proliferation and Division, and mRNA Levels of Periodic Genes in Cryptococcus neoformans
Article Snippet: .. Determining the Cell Size, Growth Curves and Budding Rate For cell size measurement, yeast strains were cultured in the YPD liquid medium at 28 °C or 37 °C, with shaking speed of 180 rpm for 48 h. Cells were harvested and the cell size was measured with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Nikon eclipse 80i fluorescence microscope
    Dynamics of immune cells infiltrating the tumors of ricolinostat and/or JQ1-treated KP mice Cell suspensions generated from tumor nodules of KP mice that were treated with vehicle, ricolinostat or JQ1 for 5–6 weeks were subjected to FACS analysis to assess proportions of CD45+ T lymphocyte subsets. (A) Proportion of CD4+, CD8+ conventional T cells, or CD4+Foxp3+ Tregs and (B) ratio of CD8 to CD4+Foxp3+ Treg cells within the tumors. Frozen sections of fresh tumor nodules from these treated KP mice were stained for TAMs (CD11c+; red) and T cells (CD3+; green), and counter stained with DAPI (nuclei; blue). (C) Representative immunofluorescent staining of tumor sections from mice treated as indicated. Images were captured on a Nikon Eclipse <t>80i</t> fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Scale bar; 25μm. * indicates p-value ˂ 0.05, ** indicates p-value ˂0.01, *** indicates p-value ˂0.001.
    Eclipse 80i Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse 80i fluorescence microscope/product/Nikon
    Average 94 stars, based on 641 article reviews
    Price from $9.99 to $1999.99
    eclipse 80i fluorescence microscope - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Dynamics of immune cells infiltrating the tumors of ricolinostat and/or JQ1-treated KP mice Cell suspensions generated from tumor nodules of KP mice that were treated with vehicle, ricolinostat or JQ1 for 5–6 weeks were subjected to FACS analysis to assess proportions of CD45+ T lymphocyte subsets. (A) Proportion of CD4+, CD8+ conventional T cells, or CD4+Foxp3+ Tregs and (B) ratio of CD8 to CD4+Foxp3+ Treg cells within the tumors. Frozen sections of fresh tumor nodules from these treated KP mice were stained for TAMs (CD11c+; red) and T cells (CD3+; green), and counter stained with DAPI (nuclei; blue). (C) Representative immunofluorescent staining of tumor sections from mice treated as indicated. Images were captured on a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Scale bar; 25μm. * indicates p-value ˂ 0.05, ** indicates p-value ˂0.01, *** indicates p-value ˂0.001.

    Journal: Cancer discovery

    Article Title: Synergistic immunostimulatory effects and therapeutic benefit of combined histone deacetylase and bromodomain inhibition in non-small cell lung cancer

    doi: 10.1158/2159-8290.CD-16-1020

    Figure Lengend Snippet: Dynamics of immune cells infiltrating the tumors of ricolinostat and/or JQ1-treated KP mice Cell suspensions generated from tumor nodules of KP mice that were treated with vehicle, ricolinostat or JQ1 for 5–6 weeks were subjected to FACS analysis to assess proportions of CD45+ T lymphocyte subsets. (A) Proportion of CD4+, CD8+ conventional T cells, or CD4+Foxp3+ Tregs and (B) ratio of CD8 to CD4+Foxp3+ Treg cells within the tumors. Frozen sections of fresh tumor nodules from these treated KP mice were stained for TAMs (CD11c+; red) and T cells (CD3+; green), and counter stained with DAPI (nuclei; blue). (C) Representative immunofluorescent staining of tumor sections from mice treated as indicated. Images were captured on a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Scale bar; 25μm. * indicates p-value ˂ 0.05, ** indicates p-value ˂0.01, *** indicates p-value ˂0.001.

    Article Snippet: Sections were imaged using a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera (Roper Scientific).

    Techniques: Mouse Assay, Generated, FACS, Staining, Fluorescence, Microscopy, Imaging, Software

    Measurement of the cell size. ( a ) Yeast strains were cultured in YPD liquid medium at 28 °C or 37 °C, with 180 rpm shaking for 48 h. Cells were harvested and observed with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ( b ). Forty cells were removed randomly from each sample to measure cell size. Data were processed using GraphPad Prism 5 software. Unpaired t -test was used for statistical analysis.

    Journal: Genes

    Article Title: Effects of 5′-3′ Exonuclease Xrn1 on Cell Size, Proliferation and Division, and mRNA Levels of Periodic Genes in Cryptococcus neoformans

    doi: 10.3390/genes11040430

    Figure Lengend Snippet: Measurement of the cell size. ( a ) Yeast strains were cultured in YPD liquid medium at 28 °C or 37 °C, with 180 rpm shaking for 48 h. Cells were harvested and observed with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan). ( b ). Forty cells were removed randomly from each sample to measure cell size. Data were processed using GraphPad Prism 5 software. Unpaired t -test was used for statistical analysis.

    Article Snippet: Determining the Cell Size, Growth Curves and Budding Rate For cell size measurement, yeast strains were cultured in the YPD liquid medium at 28 °C or 37 °C, with shaking speed of 180 rpm for 48 h. Cells were harvested and the cell size was measured with a Nikon Eclipse 80i fluorescence microscope (Nikon, Tokyo, Japan).

    Techniques: Cell Culture, Fluorescence, Microscopy, Software

    Fluorescence staining of GD3 and cholesterol and ABCA1 gene expression. ( A – B , E – F ): LM micrographs of PBLs following different treatments and labeled with anti-GD3 (A–B, GD3, green) or filipin (E–F, cholesterol, blue), taken with a fluorescence LM Eclipse 80i equipped with an illuminator Hg-C HGFIE of 130 W and DXM 1200F digital camera (Nikon), by setting a bright-field or a green (A–B, GD3)/blue (E–F, cholesterol) filter. ( C – D , G – H ): Density integrated in the green (C–D, GD3)/blue (G–I, cholesterol) channel fluorescence of LM micrographs quantified by using the image software ImageJ (US NIH) (left). In each experiment, at least 500 cells were scored. ( I ): ABCA1 gene expression levels (RT-qPCR) of PBLs following different treatments than control at the baseline time-point (0 h), by considering the 18S rRNA housekeeping gene as an internal control. The error bar represents the SE of five independent experiments, each performed in duplicate. All values plotted relate to the value of control PBLs at time 0 h (T0), taken as 100%. × indicates a significant value than control ( p

    Journal: Molecules

    Article Title: Moderate Static Magnetic Field (6 mT)-Induced Lipid Rafts Rearrangement Increases Silver NPs Uptake in Human Lymphocytes

    doi: 10.3390/molecules25061398

    Figure Lengend Snippet: Fluorescence staining of GD3 and cholesterol and ABCA1 gene expression. ( A – B , E – F ): LM micrographs of PBLs following different treatments and labeled with anti-GD3 (A–B, GD3, green) or filipin (E–F, cholesterol, blue), taken with a fluorescence LM Eclipse 80i equipped with an illuminator Hg-C HGFIE of 130 W and DXM 1200F digital camera (Nikon), by setting a bright-field or a green (A–B, GD3)/blue (E–F, cholesterol) filter. ( C – D , G – H ): Density integrated in the green (C–D, GD3)/blue (G–I, cholesterol) channel fluorescence of LM micrographs quantified by using the image software ImageJ (US NIH) (left). In each experiment, at least 500 cells were scored. ( I ): ABCA1 gene expression levels (RT-qPCR) of PBLs following different treatments than control at the baseline time-point (0 h), by considering the 18S rRNA housekeeping gene as an internal control. The error bar represents the SE of five independent experiments, each performed in duplicate. All values plotted relate to the value of control PBLs at time 0 h (T0), taken as 100%. × indicates a significant value than control ( p

    Article Snippet: Early apoptotic and apoptotic cells were recognized as annexin-V-positive using a fluorescence microscope Eclipse 80i (Nikon, Kawasaki, Kanagawa Prefecture, Japan).

    Techniques: Fluorescence, Staining, Expressing, Labeling, Software, Quantitative RT-PCR

    Triple-fluorescence staining of GD3, cholesterol and nucleus. LM micrographs of PBLs following different treatments and labeled with anti-GD3 (GD3, green); filipin (cholesterol, blue) or propidium iodide (DNA, red) taken with a fluorescence LM Eclipse 80i equipped with an illuminator Hg-C HGFIE of 130 W and DXM 1200F digital camera (Nikon), by setting a bright-field or a green (GD3)/blue (cholesterol)/red (double-stranded DNA) filter. Abbreviations: Ctrl = control PBLs, SMF = PBLs exposed to 6-mT SMF, CHX = PBLs treated with 10-mM CHX, SMF+CHX = PBLs exposed to SMF and treated simultaneously with CHX, 18-h CHX + 24-h SMF = PBLs exposed for 24 h to 6-mT SMF following treatment with CHX (10 mM) for 18 h, 24-h SMF + 18-h CHX = PBLs treated with CHX (10 mM) for 18 h following exposure for 24 h to 6-mT SMF, h = hours and bars = 10 μm.

    Journal: Molecules

    Article Title: Moderate Static Magnetic Field (6 mT)-Induced Lipid Rafts Rearrangement Increases Silver NPs Uptake in Human Lymphocytes

    doi: 10.3390/molecules25061398

    Figure Lengend Snippet: Triple-fluorescence staining of GD3, cholesterol and nucleus. LM micrographs of PBLs following different treatments and labeled with anti-GD3 (GD3, green); filipin (cholesterol, blue) or propidium iodide (DNA, red) taken with a fluorescence LM Eclipse 80i equipped with an illuminator Hg-C HGFIE of 130 W and DXM 1200F digital camera (Nikon), by setting a bright-field or a green (GD3)/blue (cholesterol)/red (double-stranded DNA) filter. Abbreviations: Ctrl = control PBLs, SMF = PBLs exposed to 6-mT SMF, CHX = PBLs treated with 10-mM CHX, SMF+CHX = PBLs exposed to SMF and treated simultaneously with CHX, 18-h CHX + 24-h SMF = PBLs exposed for 24 h to 6-mT SMF following treatment with CHX (10 mM) for 18 h, 24-h SMF + 18-h CHX = PBLs treated with CHX (10 mM) for 18 h following exposure for 24 h to 6-mT SMF, h = hours and bars = 10 μm.

    Article Snippet: Early apoptotic and apoptotic cells were recognized as annexin-V-positive using a fluorescence microscope Eclipse 80i (Nikon, Kawasaki, Kanagawa Prefecture, Japan).

    Techniques: Fluorescence, Staining, Labeling

    Efflux studies in MCF-7 and MCF-7/Twist cells. (A) Representative photomicrographs of Hoechst efflux staining in MCF-7/Twist cells compared with parental MCF-7 cells. The cells, after efflux, were photographed using a Nikon Eclipse 80i fluorescence microscope. (B) Histogram showing quantification of fluorescence intensity per cell. Cell fluorescence ( n = 6) in the blue channel was analyzed using dedicated software developed in IDL programming environment that provides an operator-free segmentation of images and determines the average relative fluorescence per cell. Three images were analyzed per sample. (C) Histogram showing expression of drug transporters ABCG2, ABCC1 , and ABCA1 in MCF-7/Twist and parental MCF-7 cells. (D) Histogram showing efflux of Rhodamine 123 stain in MCF-7/Twist and in MCF-7 cells. The amount of Rhodamine 123 dye in the cells was determined by flow cytometry. Results are representative of three separate experiments.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Twist Modulates Breast Cancer Stem Cells by Transcriptional Regulation of CD24 Expression 1

    doi:

    Figure Lengend Snippet: Efflux studies in MCF-7 and MCF-7/Twist cells. (A) Representative photomicrographs of Hoechst efflux staining in MCF-7/Twist cells compared with parental MCF-7 cells. The cells, after efflux, were photographed using a Nikon Eclipse 80i fluorescence microscope. (B) Histogram showing quantification of fluorescence intensity per cell. Cell fluorescence ( n = 6) in the blue channel was analyzed using dedicated software developed in IDL programming environment that provides an operator-free segmentation of images and determines the average relative fluorescence per cell. Three images were analyzed per sample. (C) Histogram showing expression of drug transporters ABCG2, ABCC1 , and ABCA1 in MCF-7/Twist and parental MCF-7 cells. (D) Histogram showing efflux of Rhodamine 123 stain in MCF-7/Twist and in MCF-7 cells. The amount of Rhodamine 123 dye in the cells was determined by flow cytometry. Results are representative of three separate experiments.

    Article Snippet: Sections were mounted using Permount (Thermo-Fisher Scientific, Waltham, MA) and photographed on a Nikon Eclipse 80i fluorescence microscope using a CoolSnap ES camera (Nikon Instruments).

    Techniques: Staining, Fluorescence, Microscopy, Software, Expressing, Flow Cytometry, Cytometry