Structured Review

Nikon eclipse 800 microscope
Elevated endogenous DNA damage in HCC. Immortalized hepatocyte MIHA and HCC cell lines were seeded onto 6-well culture plates with coverslips and treated (i) with or (ii) without IR (10Gy). After recovery for 6h, fixed and permeabilized cells were immunostained with (a) gamma-H2AX and 53BP1 and, (b) RAD51 and 53BP1 antibodies, followed by fluorescent-conjugated secondary antibodies. Nuclei were counterstained with DAPI. Images were captured on Nikon <t>Eclipse</t> 800 microscope.
Eclipse 800 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse 800 microscope/product/Nikon
Average 93 stars, based on 318 article reviews
Price from $9.99 to $1999.99
eclipse 800 microscope - by Bioz Stars, 2020-08
93/100 stars

Images

1) Product Images from "Preemptive Homology-Directed DNA Repair Fosters Complex Genomic Rearrangements in Hepatocellular Carcinoma"

Article Title: Preemptive Homology-Directed DNA Repair Fosters Complex Genomic Rearrangements in Hepatocellular Carcinoma

Journal: Translational Oncology

doi: 10.1016/j.tranon.2020.100796

Elevated endogenous DNA damage in HCC. Immortalized hepatocyte MIHA and HCC cell lines were seeded onto 6-well culture plates with coverslips and treated (i) with or (ii) without IR (10Gy). After recovery for 6h, fixed and permeabilized cells were immunostained with (a) gamma-H2AX and 53BP1 and, (b) RAD51 and 53BP1 antibodies, followed by fluorescent-conjugated secondary antibodies. Nuclei were counterstained with DAPI. Images were captured on Nikon Eclipse 800 microscope.
Figure Legend Snippet: Elevated endogenous DNA damage in HCC. Immortalized hepatocyte MIHA and HCC cell lines were seeded onto 6-well culture plates with coverslips and treated (i) with or (ii) without IR (10Gy). After recovery for 6h, fixed and permeabilized cells were immunostained with (a) gamma-H2AX and 53BP1 and, (b) RAD51 and 53BP1 antibodies, followed by fluorescent-conjugated secondary antibodies. Nuclei were counterstained with DAPI. Images were captured on Nikon Eclipse 800 microscope.

Techniques Used: Microscopy

2) Product Images from "Fibroblasts Expressing the Thyrotropin Receptor Overarch Thyroid and Orbit in Graves' Disease"

Article Title: Fibroblasts Expressing the Thyrotropin Receptor Overarch Thyroid and Orbit in Graves' Disease

Journal: The Journal of Clinical Endocrinology and Metabolism

doi: 10.1210/jc.2011-1249

Examination of healthy thyroid tissue, and that from patients with GD and HT reveals variable presence of CD90 + CD34 + TSHR + α-SMA + Col I + CD31 − fibrocytes. Thin tissue sections were fixed in 4% paraformaldehyde, blocked with goat serum, and incubated with primary mouse antihuman CD34, TSHR, Col I, or isotype control MoAb as described in Subjects and Methods . After washes, slides were incubated with secondary Ab. Other thin sections were stained with H E. Confocal microscopy was accomplished with a Nikon Eclipse 800 microscope interfaced with a Nikon PCM 2000 two-laser confocal system. The final magnifications were ×600 for immunostaining and ×200 for H E images.
Figure Legend Snippet: Examination of healthy thyroid tissue, and that from patients with GD and HT reveals variable presence of CD90 + CD34 + TSHR + α-SMA + Col I + CD31 − fibrocytes. Thin tissue sections were fixed in 4% paraformaldehyde, blocked with goat serum, and incubated with primary mouse antihuman CD34, TSHR, Col I, or isotype control MoAb as described in Subjects and Methods . After washes, slides were incubated with secondary Ab. Other thin sections were stained with H E. Confocal microscopy was accomplished with a Nikon Eclipse 800 microscope interfaced with a Nikon PCM 2000 two-laser confocal system. The final magnifications were ×600 for immunostaining and ×200 for H E images.

Techniques Used: Incubation, Staining, Confocal Microscopy, Microscopy, Immunostaining

3) Product Images from "Differential Expression of Thrombospondin (THBS1) in Tumorigenic and Non-Tumorigenic Prostate Epithelial Cells in Response to a Chromatin-Binding Soy Peptide"

Article Title: Differential Expression of Thrombospondin (THBS1) in Tumorigenic and Non-Tumorigenic Prostate Epithelial Cells in Response to a Chromatin-Binding Soy Peptide

Journal: Nutrition and cancer

doi: 10.1080/01635581.2011.539312

Lunasin uptake in RWPE-1 and RWPE-2 cells. RWPE-1 and RWPE-2 cells were cultured for 24 h before they were treated with 0, 20, or 50 μM lunasin. After 3 and 24 h treatment, cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin, and incubated with a lunasin polyclonal antibody (1:500) followed by an Alexa 488-conjugated goat anti-rabbit secondary antibody (1:250). Cell nuclei were stained with DAPI. Photomicrographs were obtained by a Nikon Eclipse 800 fluorescence microscope equipped with a digital camera. All images were acquired using the same integration parameters. Arrows indicate the locations of lunasin in the nucleus.
Figure Legend Snippet: Lunasin uptake in RWPE-1 and RWPE-2 cells. RWPE-1 and RWPE-2 cells were cultured for 24 h before they were treated with 0, 20, or 50 μM lunasin. After 3 and 24 h treatment, cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin, and incubated with a lunasin polyclonal antibody (1:500) followed by an Alexa 488-conjugated goat anti-rabbit secondary antibody (1:250). Cell nuclei were stained with DAPI. Photomicrographs were obtained by a Nikon Eclipse 800 fluorescence microscope equipped with a digital camera. All images were acquired using the same integration parameters. Arrows indicate the locations of lunasin in the nucleus.

Techniques Used: Cell Culture, Incubation, Staining, Fluorescence, Microscopy

Related Articles

Microscopy:

Article Title: Wnt-5a mRNA translation is suppressed by the Elav-like protein HuR in human breast epithelial cells
Article Snippet: .. Thereafter, the coverslips were washed five times in PBS, mounted in fluorescent mounting medium (DAKO), and examined and photographed in a Nikon Eclipse 800 microscope, using a 60× objective. .. Images were recorded with a scientific-grade, charge-coupled device (CCD) camera (Hamamatsu) and were analysed with HazeBuster deconvolution software (VayTek).

Article Title: Fibroblasts Expressing the Thyrotropin Receptor Overarch Thyroid and Orbit in Graves' Disease
Article Snippet: .. For confocal microscopy, images were acquired and analyzed using a Nikon Eclipse 800 microscope interfaced with a Nikon PCM 2000 two-laser confocal system as described previously ( ). ..

Article Title: Differential Expression of Thrombospondin (THBS1) in Tumorigenic and Non-Tumorigenic Prostate Epithelial Cells in Response to a Chromatin-Binding Soy Peptide
Article Snippet: .. Photomicrographs were obtained using a Nikon Eclipse 800 microscope equipped with a digital camera. ..

Article Title: Epidermal Expression of Neuropilin 1 Protects Murine keratinocytes from UVB-induced apoptosis
Article Snippet: .. Fluorescent stainings were photographed using a Nikon Eclipse 800 microscope equipped with a DXM1200 digital camera. .. TUNEL staining was performed on formalin-fixed and paraffin-embedded sections by using a staining kit (Promega, G3250) following the manufacturer's instructions.

Article Title: The function of classical and alternative non-homologous end-joining pathways in the fusion of dysfunctional telomeres
Article Snippet: .. A minimum of 100 cells with greater than four 53BP1 or γ-H2AX signals colocalized with telomere signals were imaged on a Nikon Eclipse 800 microscope. ..

Article Title: The function of classical and alternative non-homologous end-joining pathways in the fusion of dysfunctional telomeres
Article Snippet: .. Images were captured with SBIG and Photometrics CCD cameras on a Nikon Eclipse 800 microscope as described earlier and processed with MetaMorph Premier (Molecular Devices). .. For in-gel detection of telomeric length and G-stand overhang, a total of 2 × 106 cells were subjected to pulse-field electrophoresis.

Article Title: Prion Infection of Skeletal Muscle Cells and Papillae in the Tongue
Article Snippet: .. Images were visualized with epifluorescence using a Bio-Rad Radiance 2000 confocal system attached to a Nikon Eclipse 800 microscope. .. Inoculation of the HY TME agent into the lingual muscles of the tongue and the cerebrum resulted in incubation periods of 83 ± 3 days and 62 ± 2 days, respectively, in Golden Syrian hamsters (mean ± standard error of the mean).

Article Title: Preemptive Homology-Directed DNA Repair Fosters Complex Genomic Rearrangements in Hepatocellular Carcinoma
Article Snippet: .. Images were visualized and captured on Nikon Eclipse 800 microscope. .. Cell Fractionation and Western Blot Analyses Whole cell lysates (W) were prepared by lysing cells in NETN lysis buffer [20 mM Tris–HCL (pH 8.0), 100 mM NaCl, 0.5% NP-40, 1 mM EDTA, 0.5 mM NaF, 1 mM β-glycerophosphate] with Benzonase (Merck Millipore) and MgCl2 on ice for 30 minutes or until pellet dissolved.

Confocal Microscopy:

Article Title: Fibroblasts Expressing the Thyrotropin Receptor Overarch Thyroid and Orbit in Graves' Disease
Article Snippet: .. For confocal microscopy, images were acquired and analyzed using a Nikon Eclipse 800 microscope interfaced with a Nikon PCM 2000 two-laser confocal system as described previously ( ). ..

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  • 94
    Nikon epifluorescence microscope
    Neuronal MSI2 oligomers in AD Brain ( a ) Representative <t>epifluorescence</t> images of AD cortical section showing co-localization between α-MSI2 (red), NeuN (green) and α-Oligomer antibody (F11G3, blue) (white scale bar: 10 μm, magnification: 40X). ( b ) Twice zoomed image of ROI (green dashed line) highlighting localization of MSI2 oligomers in neurons (white scale bar: 5 μm, magnification 40X). ( c ) PCC of 5 ROIs in AD cortex section represented in bar graphs showing the levels of co-localization between MSI2 and F11G3 ( PCC : 0.9768 ± 0.01763), MSI2 and NeuN ( PCC : 0.7581 ± 0.1237) and F11G3 and NeuN ( PCC : 0.8004 ± 0.1149) signals. ( d ) LUT Fire image of selected ROI between MSI2 and α-Oligomer antibody (F11G3) confirming the presence of MSI2 oligomers in neurons with heterogeneous distribution in the cytoplasm (color scale bar represents pixel intensity)
    Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 1577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epifluorescence microscope/product/Nikon
    Average 94 stars, based on 1577 article reviews
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    92
    Nikon model eclipse 800 microscope
    PtdCho and PtdEtn impact on neurosphere-derived cells differentiation. ( a ) Neurosphere-derived cells cultured during 3 days under differentiation condition (control) or in the presence of liposomes of PtdCho or PtdEtn were immunostained with antibodies against βIII-tubulin (green), glial fibrillary acid protein (GFAP) (red) or Olig-2 (green). Nuclei were counterstained with DAPI (blue). Pictures were taken with Nikon Model <t>Eclipse</t> 800 microscope and are representative of independent experiments conditions. ( b – d ) Graphs represent the percentage of neuronal (βIII-tubulin), astroglial (GFAP) and oligodendroglial (nuclear-Olig2) cells after 3 days under the indicated condition of differentiation. ( e ) Western blot analysis was performed for βIII-tubulin and γ-tubulin as a control. The gels/blots displayed here are cropped, and without high-contrast (overexposure). The full-length gels and blots are included in a Supplementary Information file. ( f ) Neurosphere-derived cells were treated with 50 μM of PtdCho for 1 hour and then the media was replaced for phospholipid-free media and incubated for 3 days. ( g ) Percentage of neuronal cells (βIII-tubulin positive cells) when PtdCho was added later on, after 24 h of culture and incubated for 3 days. Immunostained were performed after 3 days of culture in each assayed conditions. Graphs are representative of at least three independent experiments. Data were presented as mean ± SEM. ***p
    Model Eclipse 800 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/model eclipse 800 microscope/product/Nikon
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    model eclipse 800 microscope - by Bioz Stars, 2020-08
    92/100 stars
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    88
    Nikon eclipse e 800 fluorescence microscope
    Emetine is an early inhibitor of HCMV replication. A)  Emetine does not inhibit HCMV entry. Cells were treated with emetine (75 nM), GCV (10 μM), and CpG 2006 (10 μM) 24 h prior to infection. Cells were infected with HCMV and treated with compounds for 90 min. Immunofluorescence staining was performed with mouse monoclonal anti-pp65 antibody. The fluorescence of rhodamine anti-mouse IgG and DAPI was visualized and merged using a Nikon Eclipse E-800 fluorescence microscope.  B)  Emetine has an early activity against HCMV. Cells were infected with HCMV Towne, and compounds were added at 0, 6, 12, 24, 36, and 48 hpi (Add on). Culture supernatants (10%) were collected at 72 hpi for a plaque assay after 14 days.  C)  Cells were infected with HCMV Towne and treated with compounds immediately after virus adsorption. Compounds were removed at 0, 6, 12, 24, 36, and 48 hpi (Removal). Culture supernatants were collected at 72 hpi for titration by plaque assay. Data represent mean ± SE of triplicate determinations from a representative of two independent experiments.
    Eclipse E 800 Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse e 800 fluorescence microscope/product/Nikon
    Average 88 stars, based on 26 article reviews
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    Image Search Results


    Neuronal MSI2 oligomers in AD Brain ( a ) Representative epifluorescence images of AD cortical section showing co-localization between α-MSI2 (red), NeuN (green) and α-Oligomer antibody (F11G3, blue) (white scale bar: 10 μm, magnification: 40X). ( b ) Twice zoomed image of ROI (green dashed line) highlighting localization of MSI2 oligomers in neurons (white scale bar: 5 μm, magnification 40X). ( c ) PCC of 5 ROIs in AD cortex section represented in bar graphs showing the levels of co-localization between MSI2 and F11G3 ( PCC : 0.9768 ± 0.01763), MSI2 and NeuN ( PCC : 0.7581 ± 0.1237) and F11G3 and NeuN ( PCC : 0.8004 ± 0.1149) signals. ( d ) LUT Fire image of selected ROI between MSI2 and α-Oligomer antibody (F11G3) confirming the presence of MSI2 oligomers in neurons with heterogeneous distribution in the cytoplasm (color scale bar represents pixel intensity)

    Journal: Acta Neuropathologica Communications

    Article Title: Formation of Toxic Oligomeric Assemblies of RNA-binding Protein: Musashi in Alzheimer’s disease

    doi: 10.1186/s40478-018-0615-0

    Figure Lengend Snippet: Neuronal MSI2 oligomers in AD Brain ( a ) Representative epifluorescence images of AD cortical section showing co-localization between α-MSI2 (red), NeuN (green) and α-Oligomer antibody (F11G3, blue) (white scale bar: 10 μm, magnification: 40X). ( b ) Twice zoomed image of ROI (green dashed line) highlighting localization of MSI2 oligomers in neurons (white scale bar: 5 μm, magnification 40X). ( c ) PCC of 5 ROIs in AD cortex section represented in bar graphs showing the levels of co-localization between MSI2 and F11G3 ( PCC : 0.9768 ± 0.01763), MSI2 and NeuN ( PCC : 0.7581 ± 0.1237) and F11G3 and NeuN ( PCC : 0.8004 ± 0.1149) signals. ( d ) LUT Fire image of selected ROI between MSI2 and α-Oligomer antibody (F11G3) confirming the presence of MSI2 oligomers in neurons with heterogeneous distribution in the cytoplasm (color scale bar represents pixel intensity)

    Article Snippet: Image analysis The slides were imaged with an epifluorescence microscope (Nikon Eclipse 800) equipped with a CoolSnap-FX monochrome CCD camera (Photometrics) using standard Nikon GFP, FITC and DAPI filters.

    Techniques: Periodic Counter-current Chromatography

    Co-localization of MSI1 and MSI2 with tau in AD Brains ( a ) Representative epifluorescence image of AD cortex section stained with α-MSI1 and Pan-tau (Tau5) antibodies (white scale bar: 50 μm, magnification: 10X). ( b ) Representative epifluorescence image of AD cortex section stained with α-MSI2 and Pan-tau (Tau5) antibodies (white scale bar: 50 μm, magnification: 10X). ( c ) Inset 1: ten times zoomed image (green square in a ) showed diffuse cytoplasmic co-localization of MSI1 and tau (white scale bar: 5 μm). Inset 2: ten times zoomed image (green square in c ) showed cytoplasmic co-localization of MSI2 and tau (white scale bar: 5 μm). ( d ) PCC Graph represent colocalization coefficient between MSI1/Tau ( PCC : 0.84 ± 0.07) and MSI2/Tau ( PCC : 0.80 ± 0.06) in positive cells to both proteins showing high association in AD brain

    Journal: Acta Neuropathologica Communications

    Article Title: Formation of Toxic Oligomeric Assemblies of RNA-binding Protein: Musashi in Alzheimer’s disease

    doi: 10.1186/s40478-018-0615-0

    Figure Lengend Snippet: Co-localization of MSI1 and MSI2 with tau in AD Brains ( a ) Representative epifluorescence image of AD cortex section stained with α-MSI1 and Pan-tau (Tau5) antibodies (white scale bar: 50 μm, magnification: 10X). ( b ) Representative epifluorescence image of AD cortex section stained with α-MSI2 and Pan-tau (Tau5) antibodies (white scale bar: 50 μm, magnification: 10X). ( c ) Inset 1: ten times zoomed image (green square in a ) showed diffuse cytoplasmic co-localization of MSI1 and tau (white scale bar: 5 μm). Inset 2: ten times zoomed image (green square in c ) showed cytoplasmic co-localization of MSI2 and tau (white scale bar: 5 μm). ( d ) PCC Graph represent colocalization coefficient between MSI1/Tau ( PCC : 0.84 ± 0.07) and MSI2/Tau ( PCC : 0.80 ± 0.06) in positive cells to both proteins showing high association in AD brain

    Article Snippet: Image analysis The slides were imaged with an epifluorescence microscope (Nikon Eclipse 800) equipped with a CoolSnap-FX monochrome CCD camera (Photometrics) using standard Nikon GFP, FITC and DAPI filters.

    Techniques: Staining, Periodic Counter-current Chromatography

    Co-localization of MSI1 and MSI2 with tau oligomers in AD Brain ( a ) Epifluorescence images of AD cortex section stained with α-MSI2 (red) and α-tau oligomeric antibody, T22 (green), DAPI (Nuclei, blue). Co-localization was observed in large portion of the tissue. Different distribution pattern was observed. Area positive to MSI1 spot distribution and negative signal of T22 (top graph) and strong co-localization was observed in same cells between MSI1 and T22 in the cytoplasm (bottom graph). The fluorescence intensity was measured and represented as intensity plot profile (DAPI, blue; MSI2, red and TauO, green). These signals have been observed in different sections of cortices (white scale bar: 10 μm magnification, 20X zoomed twice). ( b ) Epifluorescence images of AD cortex section stained with α-MSI2 (red) and α-tau oligomeric antibody, T22 (green). Different Co-localization grades were evaluated in 3 different ROIs (green squares) (white scale bar: 50 μm, magnification: 40X). Image 1 (MSI2+/TauO-), Image 2 (MSI2+/TauO+) and Image 3 (MSI2+/TauO+) showed different distribution patterns of MSI2 and tau oligomers (white scale bar: 20 μm, magnification: 40X). One ROI from each image (1–3) was selected and the fluorescence intensity was measured which is represented as intensity plot profile (DAPI, blue; MSI2, red and TauO, green)

    Journal: Acta Neuropathologica Communications

    Article Title: Formation of Toxic Oligomeric Assemblies of RNA-binding Protein: Musashi in Alzheimer’s disease

    doi: 10.1186/s40478-018-0615-0

    Figure Lengend Snippet: Co-localization of MSI1 and MSI2 with tau oligomers in AD Brain ( a ) Epifluorescence images of AD cortex section stained with α-MSI2 (red) and α-tau oligomeric antibody, T22 (green), DAPI (Nuclei, blue). Co-localization was observed in large portion of the tissue. Different distribution pattern was observed. Area positive to MSI1 spot distribution and negative signal of T22 (top graph) and strong co-localization was observed in same cells between MSI1 and T22 in the cytoplasm (bottom graph). The fluorescence intensity was measured and represented as intensity plot profile (DAPI, blue; MSI2, red and TauO, green). These signals have been observed in different sections of cortices (white scale bar: 10 μm magnification, 20X zoomed twice). ( b ) Epifluorescence images of AD cortex section stained with α-MSI2 (red) and α-tau oligomeric antibody, T22 (green). Different Co-localization grades were evaluated in 3 different ROIs (green squares) (white scale bar: 50 μm, magnification: 40X). Image 1 (MSI2+/TauO-), Image 2 (MSI2+/TauO+) and Image 3 (MSI2+/TauO+) showed different distribution patterns of MSI2 and tau oligomers (white scale bar: 20 μm, magnification: 40X). One ROI from each image (1–3) was selected and the fluorescence intensity was measured which is represented as intensity plot profile (DAPI, blue; MSI2, red and TauO, green)

    Article Snippet: Image analysis The slides were imaged with an epifluorescence microscope (Nikon Eclipse 800) equipped with a CoolSnap-FX monochrome CCD camera (Photometrics) using standard Nikon GFP, FITC and DAPI filters.

    Techniques: Staining, Fluorescence

    Musashi aggregates in AD ( a ) Representative Epifluorescence images of AD and control cortices immunolabeled with α-MSI1, DAPI (blue, for nuclei) (top) and α-Msi1 (green) and α-Oligomer antibody (F11G3, red) (bottom). An increased MSI1 oligomeric signal was noticed in the cytoplasm of AD brains cortices (white arrows) ( b ) The quantified fluorescence intensity, represented as bar graph demonstrates significantly elevated level of MSI1 signal in AD cortices compared to the controls ( N = 3 sections per condition , p = 0.007 , ** ). ( c ) α-Oligomer antibody (F11G3, red), α-MSI2 antibody (green) and DAPI (nuclei, blue) immunostaining revealed MSI2 oligomers, mostly in the cytoplasm of AD brains cortices (white arrows) ( N = 3 sections per condition and magnification: 40X, scale bar: 10 μm). ( d ) The fluorescence intensity of MSI2, represented as bar graph reveal significantly elevated levels of MSI2 signal in AD cortex compared to the control ( N = 3 sections per condition, p = 0.0001, ****)

    Journal: Acta Neuropathologica Communications

    Article Title: Formation of Toxic Oligomeric Assemblies of RNA-binding Protein: Musashi in Alzheimer’s disease

    doi: 10.1186/s40478-018-0615-0

    Figure Lengend Snippet: Musashi aggregates in AD ( a ) Representative Epifluorescence images of AD and control cortices immunolabeled with α-MSI1, DAPI (blue, for nuclei) (top) and α-Msi1 (green) and α-Oligomer antibody (F11G3, red) (bottom). An increased MSI1 oligomeric signal was noticed in the cytoplasm of AD brains cortices (white arrows) ( b ) The quantified fluorescence intensity, represented as bar graph demonstrates significantly elevated level of MSI1 signal in AD cortices compared to the controls ( N = 3 sections per condition , p = 0.007 , ** ). ( c ) α-Oligomer antibody (F11G3, red), α-MSI2 antibody (green) and DAPI (nuclei, blue) immunostaining revealed MSI2 oligomers, mostly in the cytoplasm of AD brains cortices (white arrows) ( N = 3 sections per condition and magnification: 40X, scale bar: 10 μm). ( d ) The fluorescence intensity of MSI2, represented as bar graph reveal significantly elevated levels of MSI2 signal in AD cortex compared to the control ( N = 3 sections per condition, p = 0.0001, ****)

    Article Snippet: Image analysis The slides were imaged with an epifluorescence microscope (Nikon Eclipse 800) equipped with a CoolSnap-FX monochrome CCD camera (Photometrics) using standard Nikon GFP, FITC and DAPI filters.

    Techniques: Immunolabeling, Fluorescence, Immunostaining

    PtdCho and PtdEtn impact on neurosphere-derived cells differentiation. ( a ) Neurosphere-derived cells cultured during 3 days under differentiation condition (control) or in the presence of liposomes of PtdCho or PtdEtn were immunostained with antibodies against βIII-tubulin (green), glial fibrillary acid protein (GFAP) (red) or Olig-2 (green). Nuclei were counterstained with DAPI (blue). Pictures were taken with Nikon Model Eclipse 800 microscope and are representative of independent experiments conditions. ( b – d ) Graphs represent the percentage of neuronal (βIII-tubulin), astroglial (GFAP) and oligodendroglial (nuclear-Olig2) cells after 3 days under the indicated condition of differentiation. ( e ) Western blot analysis was performed for βIII-tubulin and γ-tubulin as a control. The gels/blots displayed here are cropped, and without high-contrast (overexposure). The full-length gels and blots are included in a Supplementary Information file. ( f ) Neurosphere-derived cells were treated with 50 μM of PtdCho for 1 hour and then the media was replaced for phospholipid-free media and incubated for 3 days. ( g ) Percentage of neuronal cells (βIII-tubulin positive cells) when PtdCho was added later on, after 24 h of culture and incubated for 3 days. Immunostained were performed after 3 days of culture in each assayed conditions. Graphs are representative of at least three independent experiments. Data were presented as mean ± SEM. ***p

    Journal: Scientific Reports

    Article Title: Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells

    doi: 10.1038/s41598-017-18700-4

    Figure Lengend Snippet: PtdCho and PtdEtn impact on neurosphere-derived cells differentiation. ( a ) Neurosphere-derived cells cultured during 3 days under differentiation condition (control) or in the presence of liposomes of PtdCho or PtdEtn were immunostained with antibodies against βIII-tubulin (green), glial fibrillary acid protein (GFAP) (red) or Olig-2 (green). Nuclei were counterstained with DAPI (blue). Pictures were taken with Nikon Model Eclipse 800 microscope and are representative of independent experiments conditions. ( b – d ) Graphs represent the percentage of neuronal (βIII-tubulin), astroglial (GFAP) and oligodendroglial (nuclear-Olig2) cells after 3 days under the indicated condition of differentiation. ( e ) Western blot analysis was performed for βIII-tubulin and γ-tubulin as a control. The gels/blots displayed here are cropped, and without high-contrast (overexposure). The full-length gels and blots are included in a Supplementary Information file. ( f ) Neurosphere-derived cells were treated with 50 μM of PtdCho for 1 hour and then the media was replaced for phospholipid-free media and incubated for 3 days. ( g ) Percentage of neuronal cells (βIII-tubulin positive cells) when PtdCho was added later on, after 24 h of culture and incubated for 3 days. Immunostained were performed after 3 days of culture in each assayed conditions. Graphs are representative of at least three independent experiments. Data were presented as mean ± SEM. ***p

    Article Snippet: Microscopic analysis were carried out using confocal microscope (Zeiss LSM 880) or the Nikon Model Eclipse 800 microscope and quantitative analyzes were performed with Nikon EZ-C1 3.70 Free Viewer Software or Zen image acquisition software (Carl Zeiss).

    Techniques: Derivative Assay, Cell Culture, Microscopy, Western Blot, Incubation

    Emetine is an early inhibitor of HCMV replication. A)  Emetine does not inhibit HCMV entry. Cells were treated with emetine (75 nM), GCV (10 μM), and CpG 2006 (10 μM) 24 h prior to infection. Cells were infected with HCMV and treated with compounds for 90 min. Immunofluorescence staining was performed with mouse monoclonal anti-pp65 antibody. The fluorescence of rhodamine anti-mouse IgG and DAPI was visualized and merged using a Nikon Eclipse E-800 fluorescence microscope.  B)  Emetine has an early activity against HCMV. Cells were infected with HCMV Towne, and compounds were added at 0, 6, 12, 24, 36, and 48 hpi (Add on). Culture supernatants (10%) were collected at 72 hpi for a plaque assay after 14 days.  C)  Cells were infected with HCMV Towne and treated with compounds immediately after virus adsorption. Compounds were removed at 0, 6, 12, 24, 36, and 48 hpi (Removal). Culture supernatants were collected at 72 hpi for titration by plaque assay. Data represent mean ± SE of triplicate determinations from a representative of two independent experiments.

    Journal: PLoS Pathogens

    Article Title: Efficacy and Mechanism of Action of Low Dose Emetine against Human Cytomegalovirus

    doi: 10.1371/journal.ppat.1005717

    Figure Lengend Snippet: Emetine is an early inhibitor of HCMV replication. A) Emetine does not inhibit HCMV entry. Cells were treated with emetine (75 nM), GCV (10 μM), and CpG 2006 (10 μM) 24 h prior to infection. Cells were infected with HCMV and treated with compounds for 90 min. Immunofluorescence staining was performed with mouse monoclonal anti-pp65 antibody. The fluorescence of rhodamine anti-mouse IgG and DAPI was visualized and merged using a Nikon Eclipse E-800 fluorescence microscope. B) Emetine has an early activity against HCMV. Cells were infected with HCMV Towne, and compounds were added at 0, 6, 12, 24, 36, and 48 hpi (Add on). Culture supernatants (10%) were collected at 72 hpi for a plaque assay after 14 days. C) Cells were infected with HCMV Towne and treated with compounds immediately after virus adsorption. Compounds were removed at 0, 6, 12, 24, 36, and 48 hpi (Removal). Culture supernatants were collected at 72 hpi for titration by plaque assay. Data represent mean ± SE of triplicate determinations from a representative of two independent experiments.

    Article Snippet: A drop of mount oil containing DAPI (4,6-diamidino-2-phenylindole) (Santa Cruz) was added to the slides before visualization with a Nikon Eclipse E-800 fluorescence microscope.

    Techniques: Infection, Immunofluorescence, Staining, Fluorescence, Microscopy, Activity Assay, Plaque Assay, Adsorption, Titration