Structured Review

GE Healthcare ecl prime western blotting detection reagent
Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying <t>Amersham™</t> <t>ECL™</t> Prime Western Blotting Detection Reagent.
Ecl Prime Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecl prime western blotting detection reagent/product/GE Healthcare
Average 99 stars, based on 1789 article reviews
Price from $9.99 to $1999.99
ecl prime western blotting detection reagent - by Bioz Stars, 2020-09
99/100 stars

Images

1) Product Images from "C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells"

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0153723

Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.
Figure Legend Snippet: Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.

Techniques Used: Binding Assay, Incubation, Western Blot

2) Product Images from "Functional identification of alginate lyase from the brown alga Saccharina japonica"

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica

Journal: Scientific Reports

doi: 10.1038/s41598-019-41351-6

Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.
Figure Legend Snippet: Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.

Techniques Used: Western Blot, Electrophoresis, Staining, Purification, Recombinant, Marker

3) Product Images from "Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study"

Article Title: Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study

Journal: DARU Journal of Pharmaceutical Sciences

doi: 10.1007/s40199-018-0233-1

Specificity analysis of the selected scFvs using Immunoblotting. AGS cell lysate containing CCK2R protein was separated on 12% SDS-PAGE and then transferred onto the membrane. The membranes were incubated with purified scFv fragments: JA2 ( a ), JE2 ( b ), JC2 ( c ), JC1 ( d ), JC8 ( e ), JB3 ( f ), JA4 ( g ), JD9 ( h ), as well as, a commercial anti-CCK2R antibody as a control ( i ). Then, the membranes were stained with anti-c-myc tag and HRP-conjugated goat anti-mouse IgG antibodies and subsequently staining was developed by ECL. Arrowhead shows the location of CCK2R protein band (48 kDa) detected by scFv and control antibodies
Figure Legend Snippet: Specificity analysis of the selected scFvs using Immunoblotting. AGS cell lysate containing CCK2R protein was separated on 12% SDS-PAGE and then transferred onto the membrane. The membranes were incubated with purified scFv fragments: JA2 ( a ), JE2 ( b ), JC2 ( c ), JC1 ( d ), JC8 ( e ), JB3 ( f ), JA4 ( g ), JD9 ( h ), as well as, a commercial anti-CCK2R antibody as a control ( i ). Then, the membranes were stained with anti-c-myc tag and HRP-conjugated goat anti-mouse IgG antibodies and subsequently staining was developed by ECL. Arrowhead shows the location of CCK2R protein band (48 kDa) detected by scFv and control antibodies

Techniques Used: SDS Page, Incubation, Purification, Staining

Related Articles

Western Blot:

Article Title: The Molecular and Structural Characterization of Two Vitellogenins from the Free-Living Nematode Oscheius tipulae
Article Snippet: .. After another three washes in TBS, the proteins were detected using the ECL Plus™ Western Blotting Detection System (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA) and exposed to X-ray film at 25°C. ..

Article Title: Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study
Article Snippet: .. The membranes were washing (×3) with PBST and incubated with HRP-conjugated goat anti-mouse IgG (Abcam, Cambridge, UK) at a 1:4000 dilution for 1 h. The positive reactivity of the scFvs was visualized by an enhanced chemiluminescence system, ECL™ Prime Western blotting detection reagent (GE Healthcare, Little Chalfont, UK) [ ]. .. Specific binding of the selected scFvs was characterized through immunoblotting of the lysate of the CCK2R-positive AGS cells.

Article Title: Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae
Article Snippet: .. Proteins fused with CFP, GFP, and YFP were detected using monoclonal rabbit primary antibodies against GFP [E385] (ab32146) (Abcam, Great Britain) and the Amersham ECL Prime Western Blotting Detection Reagent kit (GE Healthcare, USA). .. Real-time PCR RNA extraction was performed with “Trizol” reagent (“Invitrogene”, USA).

Article Title: Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae
Article Snippet: .. Next, proteins were transferred onto Immobilon-P PVDF membrane (GE Healthcare, USA), reacted to antibodies against GFP [E385] (ab32146) (Abcam, Great Britain), and detected by Amersham ECL Prime Western Blotting Detection Reagent kit (GE Healthcare, USA). .. Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) [ , ] was performed with 1% agarose gel.

Article Title: Cadm1 Is a Metastasis Susceptibility Gene That Suppresses Metastasis by Modifying Tumor Interaction with the Cell-Mediated ImmunityThe Long Path from QTL to Gene
Article Snippet: .. Immunoblot was visualized using Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare). .. Densitometry data were obtained and analyzed with a ChemiDoc-It Imaging System and VisionWorksLS software (UVP).

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae
Article Snippet: .. The chemiluminescence signal was detected with an ECL Plus Western Blotting Detection System (GE Healthcare, Freiburg, Germany) and a G:BOX imaging system (Syngene, Cambridge, UK). .. Extrachromosomal rDNA circles (Southern blot analysis) To create an rDNA specific probe, the pNOY373 plasmid, a derivative of the high copy number plasmid YEp351 carrying rDNA with a promoter starting from –206 with a XhoI –NotI flanked enhancer, LEU2 , 2µ, amp , was used.

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: .. Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection .. Immunofluorescence staining Following the binding assay described above, cells were fixed with methanol for 5min at -20°C.

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica
Article Snippet: .. The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA). .. Computational analysis of SjAly SignalP 4.1 server ( www.cbs.dtu.dk/services/SignalP ) was used to predict the sequence of the signal peptide for secretion .

Incubation:

Article Title: Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study
Article Snippet: .. The membranes were washing (×3) with PBST and incubated with HRP-conjugated goat anti-mouse IgG (Abcam, Cambridge, UK) at a 1:4000 dilution for 1 h. The positive reactivity of the scFvs was visualized by an enhanced chemiluminescence system, ECL™ Prime Western blotting detection reagent (GE Healthcare, Little Chalfont, UK) [ ]. .. Specific binding of the selected scFvs was characterized through immunoblotting of the lysate of the CCK2R-positive AGS cells.

Imaging:

Article Title: Links between nucleolar activity, rDNA stability, aneuploidy and chronological aging in the yeast Saccharomyces cerevisiae
Article Snippet: .. The chemiluminescence signal was detected with an ECL Plus Western Blotting Detection System (GE Healthcare, Freiburg, Germany) and a G:BOX imaging system (Syngene, Cambridge, UK). .. Extrachromosomal rDNA circles (Southern blot analysis) To create an rDNA specific probe, the pNOY373 plasmid, a derivative of the high copy number plasmid YEp351 carrying rDNA with a promoter starting from –206 with a XhoI –NotI flanked enhancer, LEU2 , 2µ, amp , was used.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    GE Healthcare ecl prime western blotting detection reagent
    Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying <t>Amersham™</t> <t>ECL™</t> Prime Western Blotting Detection Reagent.
    Ecl Prime Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime western blotting detection reagent/product/GE Healthcare
    Average 99 stars, based on 1789 article reviews
    Price from $9.99 to $1999.99
    ecl prime western blotting detection reagent - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.

    Journal: PLoS ONE

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

    doi: 10.1371/journal.pone.0153723

    Figure Lengend Snippet: Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.

    Article Snippet: Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

    Techniques: Binding Assay, Incubation, Western Blot

    Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.

    Journal: Scientific Reports

    Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica

    doi: 10.1038/s41598-019-41351-6

    Figure Lengend Snippet: Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.

    Article Snippet: The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA).

    Techniques: Western Blot, Electrophoresis, Staining, Purification, Recombinant, Marker

    Specificity analysis of the selected scFvs using Immunoblotting. AGS cell lysate containing CCK2R protein was separated on 12% SDS-PAGE and then transferred onto the membrane. The membranes were incubated with purified scFv fragments: JA2 ( a ), JE2 ( b ), JC2 ( c ), JC1 ( d ), JC8 ( e ), JB3 ( f ), JA4 ( g ), JD9 ( h ), as well as, a commercial anti-CCK2R antibody as a control ( i ). Then, the membranes were stained with anti-c-myc tag and HRP-conjugated goat anti-mouse IgG antibodies and subsequently staining was developed by ECL. Arrowhead shows the location of CCK2R protein band (48 kDa) detected by scFv and control antibodies

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study

    doi: 10.1007/s40199-018-0233-1

    Figure Lengend Snippet: Specificity analysis of the selected scFvs using Immunoblotting. AGS cell lysate containing CCK2R protein was separated on 12% SDS-PAGE and then transferred onto the membrane. The membranes were incubated with purified scFv fragments: JA2 ( a ), JE2 ( b ), JC2 ( c ), JC1 ( d ), JC8 ( e ), JB3 ( f ), JA4 ( g ), JD9 ( h ), as well as, a commercial anti-CCK2R antibody as a control ( i ). Then, the membranes were stained with anti-c-myc tag and HRP-conjugated goat anti-mouse IgG antibodies and subsequently staining was developed by ECL. Arrowhead shows the location of CCK2R protein band (48 kDa) detected by scFv and control antibodies

    Article Snippet: The membranes were washing (×3) with PBST and incubated with HRP-conjugated goat anti-mouse IgG (Abcam, Cambridge, UK) at a 1:4000 dilution for 1 h. The positive reactivity of the scFvs was visualized by an enhanced chemiluminescence system, ECL™ Prime Western blotting detection reagent (GE Healthcare, Little Chalfont, UK) [ ].

    Techniques: SDS Page, Incubation, Purification, Staining