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GE Healthcare ecl prime western blotting detection reagent
Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying <t>Amersham™</t> <t>ECL™</t> Prime Western Blotting Detection Reagent.
Ecl Prime Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells"

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0153723

Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.
Figure Legend Snippet: Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.

Techniques Used: Binding Assay, Incubation, Western Blot

2) Product Images from "Functional identification of alginate lyase from the brown alga Saccharina japonica"

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica

Journal: Scientific Reports

doi: 10.1038/s41598-019-41351-6

Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.
Figure Legend Snippet: Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.

Techniques Used: Western Blot, Electrophoresis, Staining, Purification, Recombinant, Marker

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Centrifugation:

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Blocking Assay:

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Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: Additionally, blocking of ORF2-3 binding to LMH cells was performed following the same binding condition as applied above, except that ORF2-3 was pre-incubated with 1:50 diluted chicken serum raised against recombinant ORF2-3 or a negative serum as control for 1h at RT. .. Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

Electrophoresis:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
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Article Title: A Sortase A programmable phage display format for improved panning of Fab antibody libraries
Article Snippet: Prior to this, reduced phage samples were mixed with 4% (v/v) β-mercaptoethanol and heated at 95°C for 5 min. After electrophoresis, resolved phage proteins were transferred to a PVDF membrane (EMD Millipore). .. After washing with TBST, immunoreactive bands were developed with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and then visualized using CL-XPosure Film (Thermo Fisher Scientific).

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Incubation:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: .. Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad). .. The molecular weight and intensity of each band was determined with the Image Lab Software v5 (Bio-Rad).

Article Title: A Sortase A programmable phage display format for improved panning of Fab antibody libraries
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Activity Assay:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad). .. Additionally, the activity index of each protease-corresponding band was estimated by the calculation of a ratio between the volumetric densitometry of the fluorescent signal generated by untreated vs AEBSF-pretreated duplicates (−/+AEBSF).

Expressing:

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
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Article Title: Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells
Article Snippet: Paragraph title: Western blot assay for CALB1 protein expression ... Following washing with TBST, the blots were incubated with goat anti-rabbit immunoglobulin G horseradish peroxidase-conjugated secondary antibody (cat. no. sc-2054; 1:5,000 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at room temperature for 2 h and developed with an Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, Shanghai, China).

BIA-KA:

Article Title: Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine, et al. Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine
Article Snippet: Protein concentration was determined using the BCA Protein Assay Reagent Kit (Thermo Fisher Scientific). .. The membranes were treated with ECL Prime Western Blotting Detection Reagent (GE Healthcare), and the protein bands were detected using the ChemiDoc Imaging System (Bio‐Rad Laboratories).

Article Title: Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells
Article Snippet: The precipitated protein was collected through centrifugation at 4°C for 10 min at 12,000 × g. The protein concentrations were then determined using the bicinchoninic BCA protein assay kit (Thermo Fisher Scientific, Inc.). .. Following washing with TBST, the blots were incubated with goat anti-rabbit immunoglobulin G horseradish peroxidase-conjugated secondary antibody (cat. no. sc-2054; 1:5,000 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at room temperature for 2 h and developed with an Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, Shanghai, China).

Western Blot:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: .. Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad). .. The molecular weight and intensity of each band was determined with the Image Lab Software v5 (Bio-Rad).

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Article Snippet: .. The membranes were treated with ECL Prime Western Blotting Detection Reagent (GE Healthcare), and the protein bands were detected using the ChemiDoc Imaging System (Bio‐Rad Laboratories). .. Tumor‐bearing mice were injected via the tail vein with 150 kBq/100 μL 18 F‐FBPA.

Article Title: Colitogenic Bacteroides thetaiotaomicron antigens access host immune cells in a sulfatase-dependent manner via outer membrane vesicles
Article Snippet: .. Samples were loaded on gels for SDS page and western blotting using the Amersham ECL Prime Western Blotting Detection Reagent following manufacturer’s instructions. ..

Article Title: A Sortase A programmable phage display format for improved panning of Fab antibody libraries
Article Snippet: .. After washing with TBST, immunoreactive bands were developed with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and then visualized using CL-XPosure Film (Thermo Fisher Scientific). .. The membrane was stripped with Restore Western Blot Stripping Buffer (Thermo Fisher Scientific) per the manufacturer’s protocol, and then probed using a solution of ExtrAvidin-Peroxidase (Sigma-Aldrich) diluted 10,000-fold in 0.5× Western Blocking Reagent in TBS.

Article Title: Development of novel SUV39H2 inhibitors that exhibit growth suppressive effects in mouse xenograft models and regulate the phosphorylation of H2AX
Article Snippet: .. Nitrocellulose membrane was first incubated with each primary antibody as described in the "Antibodies" section and then protein bands were detected by incubating the secondary antibody, horseradish peroxidase (HRP)-conjugated antibodies and visualized with Amersham™ ECL™ Western Blotting Detection Reagents (GE Healthcare, GE Healthcare, Little Chalfont, UK) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, GE Healthcare, Little Chalfont, UK). ..

Article Title: Soluble urokinase-type plasminogen activator receptor and urokinase-type plasminogen activator receptor contribute to chemoresistance in leukemia
Article Snippet: .. The signals were visualized using an LAS-3000 Image Analyzer (Fujifilm, Tokyo, Japan) subsequent to the membranes being incubated with the ECL Prime Western Blotting Detection reagent (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) for 5 min at room temperature. .. Total RNA was extracted with TRIzol reagent supplied by Sigma-Aldrich (Merck KGaA; no. T9424) according to the manufacturer's protocol, then the RNA was removed using RNases supplied by Sigma-Aldrich (Merck KGaA; no. R6513) and reverse transcribed using a SuperScript III First-strand synthesis system (Invitrogen; Thermo Fisher Scientific, Inc.) to generate cDNA according to the manufacturer's protocol.

Article Title: Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
Article Snippet: .. Detection was achieved using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) on an ImageQuant LAS 400 apparatus (GE Healthcare Life Sciences). .. Total Xcc bacterial RNA was isolated using the MasterPure™ ) was used to design primers for PCR products of 100–125 bp (Table ).

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
Article Snippet: .. Specific signal was detected using Amersham ECL Prime Western Blotting Detection Reagent (Ge Healthcare, UK). .. For immunoprecipitation Immunoprecipitated proteins, 500 ng rPEDF or 140 ng pPEDF were fractionated by SDS-PAGE on a 10% gel, transferred to nitrocellulose membrane, blocked in PBS-3%BSA and incubated with either anti-PEDF antibodies (D-10, 1:500 dilution, for immunoprecipitation with anti-Phosphoserine antibodies) or anti-Phosphoserine antibodies (1:100 dilution, for immunoprecipitation with anti-PEDF antibodies).

Article Title: An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system
Article Snippet: .. Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) was used to visualize antibody-antigen complexes. .. The following primary antibodies were used in western blot analysis: Oct-4 (sc-5279; Santa Cruz Biotechnology); GATA6 (#5851), Brachyury (#81694), Nanog (#3580), phospho γ-H2A.X (#9718), and β-actin (#3700; all from Cell Signaling); GDF15 (07-217; Millipore); α-tubulin (11-250-C100; Exbio); mouse monoclonal antibody against p53 (DO-1) and mouse monoclonal antibody against MDM2 (2A9) were generously provided by Bořivoj Vojtěšek (Masaryk Memorial Cancer Institute, Brno, Czech Republic) and Stjepan Uldrijan (Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic), respectively.

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: .. Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection .. Immunofluorescence staining Following the binding assay described above, cells were fixed with methanol for 5min at -20°C.

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica
Article Snippet: .. The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA). .. Computational analysis of SjAly SignalP 4.1 server ( www.cbs.dtu.dk/services/SignalP ) was used to predict the sequence of the signal peptide for secretion .

Article Title: Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells
Article Snippet: .. Following washing with TBST, the blots were incubated with goat anti-rabbit immunoglobulin G horseradish peroxidase-conjugated secondary antibody (cat. no. sc-2054; 1:5,000 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at room temperature for 2 h and developed with an Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, Shanghai, China). .. The lentivirus-infected osteosarcoma U2OS cells were seeded into 96-well tissue culture plates at a density of 2×103 cells/well and incubated at 37°C for between 1 and 5 days.

Article Title: MicroRNA-101 inhibits the expression of Rhes, a striatal-enriched small G-protein, at the post-transcriptional level in vitro
Article Snippet: .. The protein bands on the membrane were detected by a chemiluminescence method using an Immobilon™ Western Chemiluminescent HRP substrate (Millipore) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare; Buckinghamshire, UK) and analyzed using the ImageQuant™ LAS 4000 biomolecular imager (GE Healthcare). .. Total RNA, including miRNA, was extracted from cultured cells using the mirVana miRNA isolation kit (Ambion, Austin, TX, USA) according to the manufacturer’s instructions.

Protease Inhibitor:

Article Title: LPS Induces mTORC1 and mTORC2 Activation During Monocyte Adhesion
Article Snippet: LPS (055: B5), HEPES, tris, phosphate-buffered saline (PBS), sodium bicarbonate, phenylmethane sulfonyl fluoride (PMSF), sodium orthovanadate, sodium fluoride, sodium β-glycerophosphate, sodium pyrophosphate, sodium azide, protease inhibitor, adenosine triphosphate (ATP), magnesium chloride, EDTA, histone type II-S, calphostin C and rapamycin were acquired from Sigma-Aldrich (St. Louis, M. O., USA). .. The ECL Prime Western Blotting Detection Reagent was acquired from GE Healthcare Life Sciences (Little Chalfont, Buckinghamshire, UK).

Article Title: Development of novel SUV39H2 inhibitors that exhibit growth suppressive effects in mouse xenograft models and regulate the phosphorylation of H2AX
Article Snippet: Nuclear extracts were prepared using the Nuclear Extract kit (Active Motif) to examine protein levels of SUV39H2 and H3K9me3; whole cell lysates were prepared using CelLytic™ M mammalian cell lysis reagent (Sigma-Aldrich, St. Louis, MO, USA) supplemented with a complete protease inhibitor cocktail (Roche Applied Science) and a phosphatase inhibitor cocktail (Roche Applied Science) to examine levels of apoptotic markers. .. Nitrocellulose membrane was first incubated with each primary antibody as described in the "Antibodies" section and then protein bands were detected by incubating the secondary antibody, horseradish peroxidase (HRP)-conjugated antibodies and visualized with Amersham™ ECL™ Western Blotting Detection Reagents (GE Healthcare, GE Healthcare, Little Chalfont, UK) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, GE Healthcare, Little Chalfont, UK).

Article Title: MicroRNA-101 inhibits the expression of Rhes, a striatal-enriched small G-protein, at the post-transcriptional level in vitro
Article Snippet: Cells were disrupted in cell lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 0.1% Triton, 50 mM NaF) containing a protease inhibitor cocktail (Nacalai Tesque). .. The protein bands on the membrane were detected by a chemiluminescence method using an Immobilon™ Western Chemiluminescent HRP substrate (Millipore) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare; Buckinghamshire, UK) and analyzed using the ImageQuant™ LAS 4000 biomolecular imager (GE Healthcare).

Generated:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad). .. Additionally, the activity index of each protease-corresponding band was estimated by the calculation of a ratio between the volumetric densitometry of the fluorescent signal generated by untreated vs AEBSF-pretreated duplicates (−/+AEBSF).

Imaging:

Article Title: Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine, et al. Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine
Article Snippet: .. The membranes were treated with ECL Prime Western Blotting Detection Reagent (GE Healthcare), and the protein bands were detected using the ChemiDoc Imaging System (Bio‐Rad Laboratories). .. Tumor‐bearing mice were injected via the tail vein with 150 kBq/100 μL 18 F‐FBPA.

Protein Concentration:

Article Title: Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine, et al. Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine
Article Snippet: Protein concentration was determined using the BCA Protein Assay Reagent Kit (Thermo Fisher Scientific). .. The membranes were treated with ECL Prime Western Blotting Detection Reagent (GE Healthcare), and the protein bands were detected using the ChemiDoc Imaging System (Bio‐Rad Laboratories).

Sonication:

Article Title: Colitogenic Bacteroides thetaiotaomicron antigens access host immune cells in a sulfatase-dependent manner via outer membrane vesicles
Article Snippet: Bacteria or OMVs were resuspended in PBS and sonicated. .. Samples were loaded on gels for SDS page and western blotting using the Amersham ECL Prime Western Blotting Detection Reagent following manufacturer’s instructions.

Binding Assay:

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: Paragraph title: Binding assay ... Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

Immunofluorescence:

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: For immunofluorescence staining cells were grown in ibidi 96-well μ-plates (Ibidi GmbH, Munich, Germany) and for Western blotting in 24-well plates (Cellstar ® , Greiner Bio-One GmbH, Austria). .. Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

Molecular Weight:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad). .. The molecular weight and intensity of each band was determined with the Image Lab Software v5 (Bio-Rad).

DC Protein Assay:

Article Title: An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system
Article Snippet: The protein concentration was determined using the DC Protein Assay Kit (Bio-Rad) and lysates were supplemented with bromophenol blue (0.01%) and β-2 mercaptoethanol (1%). .. Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) was used to visualize antibody-antigen complexes.

Nucleic Acid Electrophoresis:

Article Title: An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system
Article Snippet: Equal amounts of total proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) followed by transfer to a polyvinylidene fluoride (PVDF) membrane (Millipore). .. Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) was used to visualize antibody-antigen complexes.

Purification:

Article Title: Development of novel SUV39H2 inhibitors that exhibit growth suppressive effects in mouse xenograft models and regulate the phosphorylation of H2AX
Article Snippet: Nuclear extracts, whole cell lysates and purified histone extracts were separated by SDS-PAGE and blotted to nitrocellulose membrane. .. Nitrocellulose membrane was first incubated with each primary antibody as described in the "Antibodies" section and then protein bands were detected by incubating the secondary antibody, horseradish peroxidase (HRP)-conjugated antibodies and visualized with Amersham™ ECL™ Western Blotting Detection Reagents (GE Healthcare, GE Healthcare, Little Chalfont, UK) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, GE Healthcare, Little Chalfont, UK).

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica
Article Snippet: The anti-SjAly antibody was purified by affinity chromatography using an immobilized antigen formyl-cellulofine column (Seikagaku Kogyo, Tokyo, Japan). .. The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA).

Stripping:

Article Title: A Sortase A programmable phage display format for improved panning of Fab antibody libraries
Article Snippet: After washing with TBST, immunoreactive bands were developed with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and then visualized using CL-XPosure Film (Thermo Fisher Scientific). .. The membrane was stripped with Restore Western Blot Stripping Buffer (Thermo Fisher Scientific) per the manufacturer’s protocol, and then probed using a solution of ExtrAvidin-Peroxidase (Sigma-Aldrich) diluted 10,000-fold in 0.5× Western Blocking Reagent in TBS.

Polyacrylamide Gel Electrophoresis:

Article Title: An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system
Article Snippet: Equal amounts of total proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) followed by transfer to a polyvinylidene fluoride (PVDF) membrane (Millipore). .. Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) was used to visualize antibody-antigen complexes.

Staining:

Article Title: Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
Article Snippet: Similar amounts of protein were loaded into each lane of the gels, corroborated by Coomassie blue staining. .. Detection was achieved using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) on an ImageQuant LAS 400 apparatus (GE Healthcare Life Sciences).

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: For immunofluorescence staining cells were grown in ibidi 96-well μ-plates (Ibidi GmbH, Munich, Germany) and for Western blotting in 24-well plates (Cellstar ® , Greiner Bio-One GmbH, Austria). .. Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

SDS Page:

Article Title: Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine, et al. Non‐invasive estimation of 10B‐4‐borono‐L‐phenylalanine‐derived boron concentration in tumors by PET using 4‐borono‐2‐18F‐fluoro‐phenylalanine
Article Snippet: Lysates were separated by 4%‐20% SDS‐PAGE and transferred onto a PVDF membrane (Bio‐Rad Laboratories, Tokyo, Japan). .. The membranes were treated with ECL Prime Western Blotting Detection Reagent (GE Healthcare), and the protein bands were detected using the ChemiDoc Imaging System (Bio‐Rad Laboratories).

Article Title: Colitogenic Bacteroides thetaiotaomicron antigens access host immune cells in a sulfatase-dependent manner via outer membrane vesicles
Article Snippet: .. Samples were loaded on gels for SDS page and western blotting using the Amersham ECL Prime Western Blotting Detection Reagent following manufacturer’s instructions. ..

Article Title: Development of novel SUV39H2 inhibitors that exhibit growth suppressive effects in mouse xenograft models and regulate the phosphorylation of H2AX
Article Snippet: Nuclear extracts, whole cell lysates and purified histone extracts were separated by SDS-PAGE and blotted to nitrocellulose membrane. .. Nitrocellulose membrane was first incubated with each primary antibody as described in the "Antibodies" section and then protein bands were detected by incubating the secondary antibody, horseradish peroxidase (HRP)-conjugated antibodies and visualized with Amersham™ ECL™ Western Blotting Detection Reagents (GE Healthcare, GE Healthcare, Little Chalfont, UK) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, GE Healthcare, Little Chalfont, UK).

Article Title: Soluble urokinase-type plasminogen activator receptor and urokinase-type plasminogen activator receptor contribute to chemoresistance in leukemia
Article Snippet: Subsequent to each sample being mixed 1:1 with 5X Laemmli sample buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and boiled for 5 min, 20 µg of protein were separated using 7% SDS-PAGE. .. The signals were visualized using an LAS-3000 Image Analyzer (Fujifilm, Tokyo, Japan) subsequent to the membranes being incubated with the ECL Prime Western Blotting Detection reagent (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) for 5 min at room temperature.

Article Title: Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
Article Snippet: Bacterial extracts normalized by OD600 were loaded and separated in 12.5% SDS–PAGE electrophoresis. .. Detection was achieved using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) on an ImageQuant LAS 400 apparatus (GE Healthcare Life Sciences).

Article Title: Malignant ascites: a source of therapeutic protein against ovarian cancer?
Article Snippet: Proteins (10 μl FBS or ascites equivalent) were fractionated by SDS-PAGE and transferred to nitrocellulose membrane for immunoblot analysis using rabbit anti-PEDF antibodies (H-125, 1:1000 dilution from Santa Cruz Biotechnology, Germany) as primary antibodies and goat anti-rabbit IgG (H+L)-HRP conjugate (170-6515, 1:3000 dilution from Bio-Rad, Basel, Switzerland) as secondary antibodies. .. Specific signal was detected using Amersham ECL Prime Western Blotting Detection Reagent (Ge Healthcare, UK).

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica
Article Snippet: After separation by SDS-PAGE, each protein sample was transferred by electroblotting onto a nitrocellulose membrane (Atto, Tokyo, Japan). .. The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA).

Article Title: Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells
Article Snippet: Subsequently, equal amounts of total protein (30 µg/lane) were separated by 10% SDS-PAGE and transferred onto a polyvinylidene difluoride membrane. .. Following washing with TBST, the blots were incubated with goat anti-rabbit immunoglobulin G horseradish peroxidase-conjugated secondary antibody (cat. no. sc-2054; 1:5,000 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at room temperature for 2 h and developed with an Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, Shanghai, China).

Software:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad). .. The molecular weight and intensity of each band was determined with the Image Lab Software v5 (Bio-Rad).

Functional Assay:

Article Title: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Article Snippet: Paragraph title: Functional proteomic profiling ... Membranes were incubated with streptavidin-HRP (Life Technologies), and bands were visualized with ECL Prime Western Blot Detection Reagent (GE Healthcare Life Sciences) and quantified by chemiluminescence yield (Chemidoc XRS; Bio-Rad).

Recombinant:

Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells
Article Snippet: The chicken sera were obtained either from a non-inoculated specific pathogen free (SPF) bird or from an SPF bird inoculated with recombinant ORF2-3 together with GERBU Adjuvant LQ no. 3000 (GERBU Biotechnik GmbH, Heidelberg, Germany). .. Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

Affinity Chromatography:

Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica
Article Snippet: The anti-SjAly antibody was purified by affinity chromatography using an immobilized antigen formyl-cellulofine column (Seikagaku Kogyo, Tokyo, Japan). .. The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA).

Produced:

Article Title: Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
Article Snippet: Membranes were blocked with non‐fat milk in PBS and incubated with an anti‐ Xcc BphP polyclonal antibody (1:1,000) and then with anti‐mouse IgG (Fc specific)‐peroxidase antibody produced in goat (Sigma) (1:5,000). .. Detection was achieved using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) on an ImageQuant LAS 400 apparatus (GE Healthcare Life Sciences).

Lysis:

Article Title: Development of novel SUV39H2 inhibitors that exhibit growth suppressive effects in mouse xenograft models and regulate the phosphorylation of H2AX
Article Snippet: Nuclear extracts were prepared using the Nuclear Extract kit (Active Motif) to examine protein levels of SUV39H2 and H3K9me3; whole cell lysates were prepared using CelLytic™ M mammalian cell lysis reagent (Sigma-Aldrich, St. Louis, MO, USA) supplemented with a complete protease inhibitor cocktail (Roche Applied Science) and a phosphatase inhibitor cocktail (Roche Applied Science) to examine levels of apoptotic markers. .. Nitrocellulose membrane was first incubated with each primary antibody as described in the "Antibodies" section and then protein bands were detected by incubating the secondary antibody, horseradish peroxidase (HRP)-conjugated antibodies and visualized with Amersham™ ECL™ Western Blotting Detection Reagents (GE Healthcare, GE Healthcare, Little Chalfont, UK) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, GE Healthcare, Little Chalfont, UK).

Article Title: An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system
Article Snippet: For western blotting, harvested cells were washed three times with PBS, lysed in lysis buffer [50 mM Tris-HCl (pH 6.8), 1% sodium dodecyl sulfate (SDS), 10% glycerol] and stored at −80°C until use. .. Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) was used to visualize antibody-antigen complexes.

Article Title: MicroRNA-101 inhibits the expression of Rhes, a striatal-enriched small G-protein, at the post-transcriptional level in vitro
Article Snippet: Cells were disrupted in cell lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 0.1% Triton, 50 mM NaF) containing a protease inhibitor cocktail (Nacalai Tesque). .. The protein bands on the membrane were detected by a chemiluminescence method using an Immobilon™ Western Chemiluminescent HRP substrate (Millipore) or Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare; Buckinghamshire, UK) and analyzed using the ImageQuant™ LAS 4000 biomolecular imager (GE Healthcare).

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    GE Healthcare ecl prime western blotting detection reagent
    Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying <t>Amersham™</t> <t>ECL™</t> Prime Western Blotting Detection Reagent.
    Ecl Prime Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.

    Journal: PLoS ONE

    Article Title: C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells

    doi: 10.1371/journal.pone.0153723

    Figure Lengend Snippet: Semi-quantification of ORF2-3 binding to LMH cells. Different concentrations of ORF2-3 were incubated with cells for 1h. Binding was analyzed by Western blotting applying Amersham™ ECL™ Prime Western Blotting Detection Reagent.

    Article Snippet: Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, Bukinghamshire, UK) was applied for semi-quantitative analysis and SuperSignal™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA) was used for qualitative detection

    Techniques: Binding Assay, Incubation, Western Blot

    Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.

    Journal: Scientific Reports

    Article Title: Functional identification of alginate lyase from the brown alga Saccharina japonica

    doi: 10.1038/s41598-019-41351-6

    Figure Lengend Snippet: Western blot analysis of SjAly from Saccharina japonica . Each sample was applied to one gel in order of symmetry. After electrophoresis, the gel was cut in the middle, one stained with Coomassie Brilliant Blue and the other transferred to a nitrocellulose filter for western blotting. The primary antibody was diluted 1,000 times with 1 × TBS (20 mM Tris-HCl (pH 7.5) and 150 mM NaCl) and used, and the secondary antibody was similarly diluted 10,000 times and used. Protein bands were visualized using an ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA) and chemi-luminescence imager (EZ-capture MG, ATTO, Tokyo, Japan) in an auto exposure mode. An overexposed image is represented in Supplementary Fig. S8 . ( a ) SDS-polyacrylamide gel stained using Coomassie Brilliant Blue. ( b ) Western blot analysis using anti-SjAly antibodies. rSjAly , purified recombinant SjAly; Marker , protein ladder; Blade , protein extract from the blade; Stipe , protein extract from the stipe; Rhizoid , protein extract from the rhizoid. Full-length gel and blot are presented here.

    Article Snippet: The reaction signal was detected using ECL prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA).

    Techniques: Western Blot, Electrophoresis, Staining, Purification, Recombinant, Marker

    Specificity analysis of the selected scFvs using Immunoblotting. AGS cell lysate containing CCK2R protein was separated on 12% SDS-PAGE and then transferred onto the membrane. The membranes were incubated with purified scFv fragments: JA2 ( a ), JE2 ( b ), JC2 ( c ), JC1 ( d ), JC8 ( e ), JB3 ( f ), JA4 ( g ), JD9 ( h ), as well as, a commercial anti-CCK2R antibody as a control ( i ). Then, the membranes were stained with anti-c-myc tag and HRP-conjugated goat anti-mouse IgG antibodies and subsequently staining was developed by ECL. Arrowhead shows the location of CCK2R protein band (48 kDa) detected by scFv and control antibodies

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study

    doi: 10.1007/s40199-018-0233-1

    Figure Lengend Snippet: Specificity analysis of the selected scFvs using Immunoblotting. AGS cell lysate containing CCK2R protein was separated on 12% SDS-PAGE and then transferred onto the membrane. The membranes were incubated with purified scFv fragments: JA2 ( a ), JE2 ( b ), JC2 ( c ), JC1 ( d ), JC8 ( e ), JB3 ( f ), JA4 ( g ), JD9 ( h ), as well as, a commercial anti-CCK2R antibody as a control ( i ). Then, the membranes were stained with anti-c-myc tag and HRP-conjugated goat anti-mouse IgG antibodies and subsequently staining was developed by ECL. Arrowhead shows the location of CCK2R protein band (48 kDa) detected by scFv and control antibodies

    Article Snippet: The membranes were washing (×3) with PBST and incubated with HRP-conjugated goat anti-mouse IgG (Abcam, Cambridge, UK) at a 1:4000 dilution for 1 h. The positive reactivity of the scFvs was visualized by an enhanced chemiluminescence system, ECL™ Prime Western blotting detection reagent (GE Healthcare, Little Chalfont, UK) [ ].

    Techniques: SDS Page, Incubation, Purification, Staining