ecis z θ instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z θ instrument
    Ecis Z θ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta instrument
    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® <t>Z‐Theta</t> instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta instrument - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Group A Streptococcus strains causing meningitis without distinct invasive phenotype"

    Article Title: Group A Streptococcus strains causing meningitis without distinct invasive phenotype

    Journal: MicrobiologyOpen

    doi: 10.1002/mbo3.1394

    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Figure Legend Snippet: Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.

    Techniques Used: Infection, Disruption, Lysis, Activity Assay, Generated, Bacteria, Standard Deviation, MANN-WHITNEY

    ecis z theta instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta instrument
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta instrument
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z θ instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z θ instrument
    Ecis Z θ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    instrument ecis zθ theta  (Applied BioPhysics)


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    Applied BioPhysics instrument ecis zθ theta
    Instrument Ecis Zθ Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis ztheta instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis ztheta instrument
    Ecis Ztheta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis instrument zθ  (Applied BioPhysics)


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    Applied BioPhysics ecis instrument zθ
    (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing <t>(ECIS;</t> n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).
    Ecis Instrument Zθ, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Aging-regulated TUG1 is dispensable for endothelial cell function"

    Article Title: Aging-regulated TUG1 is dispensable for endothelial cell function

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0265160

    (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing (ECIS; n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).
    Figure Legend Snippet: (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing (ECIS; n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).

    Techniques Used: Transfection, Expressing, Quantitative RT-PCR, Cell Counting, Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Injection

    ecis instrument z theta model  (Applied BioPhysics)


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    Applied BioPhysics ecis instrument z theta model
    Ecis Instrument Z Theta Model, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis zθ instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis zθ instrument
    Ecis Zθ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta instrument
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics ecis z θ instrument
    Ecis Z θ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics ecis z theta instrument
    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® <t>Z‐Theta</t> instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis z theta instrument/product/Applied BioPhysics
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    96
    Applied BioPhysics instrument ecis zθ theta
    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® <t>Z‐Theta</t> instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Instrument Ecis Zθ Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics ecis ztheta instrument
    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® <t>Z‐Theta</t> instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Ecis Ztheta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Applied BioPhysics ecis instrument zθ
    (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing <t>(ECIS;</t> n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).
    Ecis Instrument Zθ, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis instrument zθ - by Bioz Stars, 2024-07
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    96
    Applied BioPhysics ecis instrument z theta model
    (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing <t>(ECIS;</t> n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).
    Ecis Instrument Z Theta Model, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis instrument z theta model/product/Applied BioPhysics
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecis instrument z theta model - by Bioz Stars, 2024-07
    96/100 stars
      Buy from Supplier

    96
    Applied BioPhysics ecis zθ instrument
    (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing <t>(ECIS;</t> n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).
    Ecis Zθ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis zθ instrument/product/Applied BioPhysics
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecis zθ instrument - by Bioz Stars, 2024-07
    96/100 stars
      Buy from Supplier

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    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.

    Journal: MicrobiologyOpen

    Article Title: Group A Streptococcus strains causing meningitis without distinct invasive phenotype

    doi: 10.1002/mbo3.1394

    Figure Lengend Snippet: Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.

    Article Snippet: ECIS® was performed with the ECIS® Z‐Theta instrument (Applied Biophysics) to monitor the effects of the GAS strains on human brain microvascular endothelial cells (HBMECs) (Nizet et al., ) barrier integrity.

    Techniques: Infection, Disruption, Lysis, Activity Assay, Generated, Bacteria, Standard Deviation, MANN-WHITNEY

    (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing (ECIS; n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).

    Journal: PLoS ONE

    Article Title: Aging-regulated TUG1 is dispensable for endothelial cell function

    doi: 10.1371/journal.pone.0265160

    Figure Lengend Snippet: (A)–(G) HUVECs were transfected with two LNA GapmeRs against TUG1 —LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl (10 nM) and (A) expression levels were measured after 48 hours by RT-qPCR. Expression is relative to GAPDH (n = 4; SEM; RM one-way ANOVA with Greenhouse-Geisser correction and Sidak multiple comparison test). (B) Relative cell growth determined from cell count at 0 h, 24 h, 48 h and 72 h (n = 3; SEM; RM Two-way ANOVA with Tuckey multiple comparison test). (C) Caspase-3/7 activity was measured by determination of fluorescence with ELISA plate reader (n = 3; SEM; One-way ANOVA with Holm-Sidak correction). Staurosporine was taken along as a postive control. (D) Cell-cell interactions (Rb) and cell-matrix-interactions (α) were measured by Electric Cell Impedance Sensing (ECIS; n = 3; SEM; Kruskal-Wallis-test with Dunn´s correction). (E) Determination of re-establishment of monolayer after wounding using ECIS (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test). (F) Seahorse mitochondrial stress test assessing multiple mitochondrial characteristics via measurement of changes in Oxygen Consumption Rate (OCR) after serial injection of Oligomycin, Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) and Rotenone A/Antimycin (n = 3; SEM; One-way ANOVA with Holm-Sidak multiple comparison test. One representative experiment displaying the changes of OCR throughout the progress of the Seahorse mitochondrial stress test assay. (G) Assessment of monocyte adhesion with and without TNF-α stimulation. (n = 3; SEM; Two-way ANOVA with Tuckey multiple comparison test).

    Article Snippet: The resulting potential was detected by the ECIS instrument Zθ (Applied BioPhysics).

    Techniques: Transfection, Expressing, Quantitative RT-PCR, Cell Counting, Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Injection