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MAL expression in EEC <t>and</t> <t>ECC-1</t> cells. A , B Expression of MAL protein in EEC and ECC-1 cells. C , D Validation of MAL protein expression knockdown and overexpression in ECC-1 cells. E , F EdU assay results for ECC-1 cell proliferation after MAL silencing or overexpression. G , H Transwell invasion assay after MAL silencing or overexpression. *, **, and ***: p < 0.05, p < 0.01, and p < 0.0001, respectively
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MAL expression in EEC and ECC-1 cells. A , B Expression of MAL protein in EEC and ECC-1 cells. C , D Validation of MAL protein expression knockdown and overexpression in ECC-1 cells. E , F EdU assay results for ECC-1 cell proliferation after MAL silencing or overexpression. G , H Transwell invasion assay after MAL silencing or overexpression. *, **, and ***: p < 0.05, p < 0.01, and p < 0.0001, respectively

Journal: BMC Cancer

Article Title: Identification of endometrial cancer biomarkers using weighted gene coexpression network analysis and machine learning

doi: 10.1186/s12885-026-15573-7

Figure Lengend Snippet: MAL expression in EEC and ECC-1 cells. A , B Expression of MAL protein in EEC and ECC-1 cells. C , D Validation of MAL protein expression knockdown and overexpression in ECC-1 cells. E , F EdU assay results for ECC-1 cell proliferation after MAL silencing or overexpression. G , H Transwell invasion assay after MAL silencing or overexpression. *, **, and ***: p < 0.05, p < 0.01, and p < 0.0001, respectively

Article Snippet: EEC and ECC-1 cells were obtained from Procell Life Science and cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37 °C in a humidified 5% CO2 incubator.

Techniques: Expressing, Biomarker Discovery, Knockdown, Over Expression, EdU Assay, Transwell Invasion Assay