Structured Review

Thermo Fisher ec top10
Ec Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ec top10/product/Thermo Fisher
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ec top10 - by Bioz Stars, 2020-03
79/100 stars

Related Products / Commonly Used Together

11168 hipo locus
jl1101
pcr2.1-topo
primers jl1100

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Related Articles

Clone Assay:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications. .. A 1.9 kb Eco RI fragment containing the hipO gene was excised from the above engineered plasmid, ligated into the Eco RI site of pBluescript II SK(+) (Stratagene), and transformed into Escherichia coli DH5α (Life Technologies) to produce plasmid pDWhipO .

Amplification:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications. .. The Campylobacter aphA -3 kanamycin marker ( ) was excised from pILL600 by Sma I and blunt-end ligated into the Sph I site of pDWhipO (polished with T4 DNA polymerase), generating pDWhipO K. Both pDWhipO K and pJB23SK were amplified in E. coli DH5α before electroporation into C. jejuni .

Southern Blot:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: Recombination occurred by double-crossover of a kanamycin or chloramphenicol resistance marker ( ) with one integration per mutant as demonstrated by Southern blotting (data not shown). .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Electroporation:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: DNA was introduced into competent C. jejuni by electroporation as described by . .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Mutagenesis:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: Recombination occurred by double-crossover of a kanamycin or chloramphenicol resistance marker ( ) with one integration per mutant as demonstrated by Southern blotting (data not shown). .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Plasmid Preparation:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: The suicide vector pDWhipO K, produced by methods similar to those utilized by , was used to introduce the kanamycin marker into the C. jejuni 11168 hipO chromosomal locus. .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Introduce:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: The suicide vector pDWhipO K, produced by methods similar to those utilized by , was used to introduce the kanamycin marker into the C. jejuni 11168 hipO chromosomal locus. .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Produced:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: The suicide vector pDWhipO K, produced by methods similar to those utilized by , was used to introduce the kanamycin marker into the C. jejuni 11168 hipO chromosomal locus. .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Homologous Recombination:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: Genetically marked C. jejuni strains were derived from 11168 through homologous recombination with transposed chromosomal DNA or with a pUC-based suicide vector. .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Marker:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: The suicide vector pDWhipO K, produced by methods similar to those utilized by , was used to introduce the kanamycin marker into the C. jejuni 11168 hipO chromosomal locus. .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

Transformation Assay:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications. .. A 1.9 kb Eco RI fragment containing the hipO gene was excised from the above engineered plasmid, ligated into the Eco RI site of pBluescript II SK(+) (Stratagene), and transformed into Escherichia coli DH5α (Life Technologies) to produce plasmid pDWhipO .

Derivative Assay:

Article Title: Genetic diversity in Campylobacter jejuni is associated with differential colonization of broiler chickens and C57BL/6J IL10-deficient mice
Article Snippet: Genetically marked C. jejuni strains were derived from 11168 through homologous recombination with transposed chromosomal DNA or with a pUC-based suicide vector. .. Briefly, primers JL1100 (5′-AGAGCTAATGATACCCTACC-3′) and JL1101 (5′-ATGCTATTGTCTGGAGGAGC-3′) were used to amplify the 11168 hipO locus (1.9 kb), which was subsequently cloned into pCR2.1-Topo (Invitrogen) and electroporated into EC TOP10 (Invitrogen) according to the manufacturer's specifications.

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  • 99
    Thermo Fisher escherichia coli
    Representative SDS-PAGE, 2-dimensional electrophoresis (2DE), and Western blots showing the workflow for selection of immunoreactive spots. (A) The 1-dimensional SDS-PAGE image of Staphylococcus aureus Newbould 305 trypsinized protein (lane 1) and <t>Escherichia</t> coli trypsinized protein (lane 2). (B) 2DE gel conducted on 7 cm, pH 4 to 7, immobilized pH gradient (IPG) strips of S. aureus Newbould 305 trypsinized protein. Mr = molecular weight. (C) Immunoblot of S. aureus Newbould 305 trypsinized protein using pooled bovine mastitic milk, with immunoreactive regions highlighted in red circles. (D) 2DE gel conducted on 7 cm, pH 4 to 7, IPG strips of Escherichia coli DH10-β trypsinized protein. (E) Immunoblot of E. coli DH10-β trypsinized protein using bovine mastitic milk, with the immunoreactive regions highlighted in red circles. Color version available online.
    Escherichia Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli/product/Thermo Fisher
    Average 99 stars, based on 389 article reviews
    Price from $9.99 to $1999.99
    escherichia coli - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Representative SDS-PAGE, 2-dimensional electrophoresis (2DE), and Western blots showing the workflow for selection of immunoreactive spots. (A) The 1-dimensional SDS-PAGE image of Staphylococcus aureus Newbould 305 trypsinized protein (lane 1) and Escherichia coli trypsinized protein (lane 2). (B) 2DE gel conducted on 7 cm, pH 4 to 7, immobilized pH gradient (IPG) strips of S. aureus Newbould 305 trypsinized protein. Mr = molecular weight. (C) Immunoblot of S. aureus Newbould 305 trypsinized protein using pooled bovine mastitic milk, with immunoreactive regions highlighted in red circles. (D) 2DE gel conducted on 7 cm, pH 4 to 7, IPG strips of Escherichia coli DH10-β trypsinized protein. (E) Immunoblot of E. coli DH10-β trypsinized protein using bovine mastitic milk, with the immunoreactive regions highlighted in red circles. Color version available online.

    Journal: Journal of dairy science

    Article Title: Immunoproteomics to identify Staphylococcus aureus antigens expressed in bovine milk during mastitis

    doi: 10.3168/jds.2017-14040

    Figure Lengend Snippet: Representative SDS-PAGE, 2-dimensional electrophoresis (2DE), and Western blots showing the workflow for selection of immunoreactive spots. (A) The 1-dimensional SDS-PAGE image of Staphylococcus aureus Newbould 305 trypsinized protein (lane 1) and Escherichia coli trypsinized protein (lane 2). (B) 2DE gel conducted on 7 cm, pH 4 to 7, immobilized pH gradient (IPG) strips of S. aureus Newbould 305 trypsinized protein. Mr = molecular weight. (C) Immunoblot of S. aureus Newbould 305 trypsinized protein using pooled bovine mastitic milk, with immunoreactive regions highlighted in red circles. (D) 2DE gel conducted on 7 cm, pH 4 to 7, IPG strips of Escherichia coli DH10-β trypsinized protein. (E) Immunoblot of E. coli DH10-β trypsinized protein using bovine mastitic milk, with the immunoreactive regions highlighted in red circles. Color version available online.

    Article Snippet: Briefly, S. aureus Newbould 305, S. aureus C1, and Escherichia coli (DH10-β Top10, Thermo Fisher Scientific, Waltham, MA) were grown in LIM overnight to an optical density ( OD ) of 0.75 to 1.2 and harvested by centrifugation (6,000 × g for 10 min at 4°C), before washing 3 times with 1× PBS.

    Techniques: SDS Page, Electrophoresis, Two-Dimensional Gel Electrophoresis, Western Blot, Selection, Molecular Weight