easy nlc nanohplc  (Thermo Fisher)


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    Structured Review

    Thermo Fisher easy nlc nanohplc
    Easy Nlc Nanohplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easy nlc nanohplc/product/Thermo Fisher
    Average 90 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    easy nlc nanohplc - by Bioz Stars, 2020-09
    90/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Mll3 and Mll4 facilitate enhancer RNA synthesis and transcription from promoters independently of H3K4 monomethylation
    Article Snippet: .. For LC-MS analysis, samples were then separated using a 75 μm ID × 17 cm Reprosil-Pur C18-AQ (3 μm; Dr. Maisch GmbH, Germany) nano-column mounted on an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, Ca, USA) in a HPLC gradient of 2% to 28% solvent B (A = 0.1% formic acid; B = 95% MeCN, 0.1% formic acid) over 45 minutes, followed by 28% to 80% solvent B in 5 minutes and 80% B for 10 more minutes at a flow-rate of 300 nL/min. nLC was coupled online to an LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific) and data were acquired using data-independent acquisition (DIA) ( ). .. Briefly, full scan MS ( m/z 300-1100) was acquired in the Orbitrap with a resolution of 120,000 (at 200 m/z) and an AGC target of 5×10e5.

    Flow Cytometry:

    Article Title: Mll3 and Mll4 facilitate enhancer RNA synthesis and transcription from promoters independently of H3K4 monomethylation
    Article Snippet: .. For LC-MS analysis, samples were then separated using a 75 μm ID × 17 cm Reprosil-Pur C18-AQ (3 μm; Dr. Maisch GmbH, Germany) nano-column mounted on an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, Ca, USA) in a HPLC gradient of 2% to 28% solvent B (A = 0.1% formic acid; B = 95% MeCN, 0.1% formic acid) over 45 minutes, followed by 28% to 80% solvent B in 5 minutes and 80% B for 10 more minutes at a flow-rate of 300 nL/min. nLC was coupled online to an LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific) and data were acquired using data-independent acquisition (DIA) ( ). .. Briefly, full scan MS ( m/z 300-1100) was acquired in the Orbitrap with a resolution of 120,000 (at 200 m/z) and an AGC target of 5×10e5.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Mll3 and Mll4 facilitate enhancer RNA synthesis and transcription from promoters independently of H3K4 monomethylation
    Article Snippet: .. For LC-MS analysis, samples were then separated using a 75 μm ID × 17 cm Reprosil-Pur C18-AQ (3 μm; Dr. Maisch GmbH, Germany) nano-column mounted on an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, Ca, USA) in a HPLC gradient of 2% to 28% solvent B (A = 0.1% formic acid; B = 95% MeCN, 0.1% formic acid) over 45 minutes, followed by 28% to 80% solvent B in 5 minutes and 80% B for 10 more minutes at a flow-rate of 300 nL/min. nLC was coupled online to an LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific) and data were acquired using data-independent acquisition (DIA) ( ). .. Briefly, full scan MS ( m/z 300-1100) was acquired in the Orbitrap with a resolution of 120,000 (at 200 m/z) and an AGC target of 5×10e5.

    Mass Spectrometry:

    Article Title: Mll3 and Mll4 facilitate enhancer RNA synthesis and transcription from promoters independently of H3K4 monomethylation
    Article Snippet: .. For LC-MS analysis, samples were then separated using a 75 μm ID × 17 cm Reprosil-Pur C18-AQ (3 μm; Dr. Maisch GmbH, Germany) nano-column mounted on an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, Ca, USA) in a HPLC gradient of 2% to 28% solvent B (A = 0.1% formic acid; B = 95% MeCN, 0.1% formic acid) over 45 minutes, followed by 28% to 80% solvent B in 5 minutes and 80% B for 10 more minutes at a flow-rate of 300 nL/min. nLC was coupled online to an LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific) and data were acquired using data-independent acquisition (DIA) ( ). .. Briefly, full scan MS ( m/z 300-1100) was acquired in the Orbitrap with a resolution of 120,000 (at 200 m/z) and an AGC target of 5×10e5.

    Article Title: Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells
    Article Snippet: .. Reversed phase nano chromatography coupled to mass spectrometry Samples were analyzed by using a nanoLC-MS/MS setup. nanoLC was configured with a 75 μm ID × 17 cm Reprosil-Pur C18-AQ (3 μm; Dr. Maisch GmbH, Germany) nano-column using an EASY-nLC nanoHPLC (Thermo Scientific, Odense, Denmark). .. The HPLC gradient was 2-35 % solvent B (A = 0.1 % formic acid; B = 95 % MeCN, 0.1 % formic acid) over 30 min and from 34 % to 100 % solvent B in 30 minutes at a flow-rate of 300 nL/min.

    Chromatography:

    Article Title: Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells
    Article Snippet: .. Reversed phase nano chromatography coupled to mass spectrometry Samples were analyzed by using a nanoLC-MS/MS setup. nanoLC was configured with a 75 μm ID × 17 cm Reprosil-Pur C18-AQ (3 μm; Dr. Maisch GmbH, Germany) nano-column using an EASY-nLC nanoHPLC (Thermo Scientific, Odense, Denmark). .. The HPLC gradient was 2-35 % solvent B (A = 0.1 % formic acid; B = 95 % MeCN, 0.1 % formic acid) over 30 min and from 34 % to 100 % solvent B in 30 minutes at a flow-rate of 300 nL/min.

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    Thermo Fisher nanohplc
    LC-ESI-MS/MS analysis of fengycins from Bacillus amyloliquefaciens MEP 2 18. Elution profile of fengycins (A) , obtained from a HPLC-purified peak after separation of CLP from B. amyloliquefaciens MEP 2 18, using <t>nanoHPLC</t> technology (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific). ESI-MS/MS spectra of protonated fengycin ions m/z 1463.8 (B) obtained from peak 1, the spectrum of precursor ion of m/z 1463.8 (C) ; m/z 1477.8 (D) obtained from peak 2, the spectrum of precursor ion of m/z 1477.8 (E) ; and ions m/z 1477.8, 1491.8, and 1505.8 (F) obtained from peak 3, the spectrum of precursor ion of m/z 1477.8 (G) , 1491.8 (H) , and 1505.8 (I) were acquired in a mass spectrometer (Thermo Scientific, Q-Exactive) with a high collision dissociation (HCD) cell and an Orbitrap analyzer.
    Nanohplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanohplc/product/Thermo Fisher
    Average 89 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    nanohplc - by Bioz Stars, 2020-09
    89/100 stars
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    93
    Thermo Fisher nanohplc system easy nlc ii proxeon system
    LC-ESI-MS/MS analysis of fengycins from Bacillus amyloliquefaciens MEP 2 18. Elution profile of fengycins (A) , obtained from a HPLC-purified peak after separation of CLP from B. amyloliquefaciens MEP 2 18, using <t>nanoHPLC</t> technology (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific). ESI-MS/MS spectra of protonated fengycin ions m/z 1463.8 (B) obtained from peak 1, the spectrum of precursor ion of m/z 1463.8 (C) ; m/z 1477.8 (D) obtained from peak 2, the spectrum of precursor ion of m/z 1477.8 (E) ; and ions m/z 1477.8, 1491.8, and 1505.8 (F) obtained from peak 3, the spectrum of precursor ion of m/z 1477.8 (G) , 1491.8 (H) , and 1505.8 (I) were acquired in a mass spectrometer (Thermo Scientific, Q-Exactive) with a high collision dissociation (HCD) cell and an Orbitrap analyzer.
    Nanohplc System Easy Nlc Ii Proxeon System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanohplc system easy nlc ii proxeon system/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nanohplc system easy nlc ii proxeon system - by Bioz Stars, 2020-09
    93/100 stars
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    90
    Thermo Fisher easy nlc 1000 nanouhplc
    LC-ESI-MS/MS analysis of fengycins from Bacillus amyloliquefaciens MEP 2 18. Elution profile of fengycins (A) , obtained from a HPLC-purified peak after separation of CLP from B. amyloliquefaciens MEP 2 18, using <t>nanoHPLC</t> technology (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific). ESI-MS/MS spectra of protonated fengycin ions m/z 1463.8 (B) obtained from peak 1, the spectrum of precursor ion of m/z 1463.8 (C) ; m/z 1477.8 (D) obtained from peak 2, the spectrum of precursor ion of m/z 1477.8 (E) ; and ions m/z 1477.8, 1491.8, and 1505.8 (F) obtained from peak 3, the spectrum of precursor ion of m/z 1477.8 (G) , 1491.8 (H) , and 1505.8 (I) were acquired in a mass spectrometer (Thermo Scientific, Q-Exactive) with a high collision dissociation (HCD) cell and an Orbitrap analyzer.
    Easy Nlc 1000 Nanouhplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easy nlc 1000 nanouhplc/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    easy nlc 1000 nanouhplc - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher easy nlc nanohplc
    LC-ESI-MS/MS analysis of fengycins from Bacillus amyloliquefaciens MEP 2 18. Elution profile of fengycins (A) , obtained from a HPLC-purified peak after separation of CLP from B. amyloliquefaciens MEP 2 18, using <t>nanoHPLC</t> technology (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific). ESI-MS/MS spectra of protonated fengycin ions m/z 1463.8 (B) obtained from peak 1, the spectrum of precursor ion of m/z 1463.8 (C) ; m/z 1477.8 (D) obtained from peak 2, the spectrum of precursor ion of m/z 1477.8 (E) ; and ions m/z 1477.8, 1491.8, and 1505.8 (F) obtained from peak 3, the spectrum of precursor ion of m/z 1477.8 (G) , 1491.8 (H) , and 1505.8 (I) were acquired in a mass spectrometer (Thermo Scientific, Q-Exactive) with a high collision dissociation (HCD) cell and an Orbitrap analyzer.
    Easy Nlc Nanohplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easy nlc nanohplc/product/Thermo Fisher
    Average 90 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    easy nlc nanohplc - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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    LC-ESI-MS/MS analysis of fengycins from Bacillus amyloliquefaciens MEP 2 18. Elution profile of fengycins (A) , obtained from a HPLC-purified peak after separation of CLP from B. amyloliquefaciens MEP 2 18, using nanoHPLC technology (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific). ESI-MS/MS spectra of protonated fengycin ions m/z 1463.8 (B) obtained from peak 1, the spectrum of precursor ion of m/z 1463.8 (C) ; m/z 1477.8 (D) obtained from peak 2, the spectrum of precursor ion of m/z 1477.8 (E) ; and ions m/z 1477.8, 1491.8, and 1505.8 (F) obtained from peak 3, the spectrum of precursor ion of m/z 1477.8 (G) , 1491.8 (H) , and 1505.8 (I) were acquired in a mass spectrometer (Thermo Scientific, Q-Exactive) with a high collision dissociation (HCD) cell and an Orbitrap analyzer.

    Journal: Frontiers in Microbiology

    Article Title: Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

    doi: 10.3389/fmicb.2019.03107

    Figure Lengend Snippet: LC-ESI-MS/MS analysis of fengycins from Bacillus amyloliquefaciens MEP 2 18. Elution profile of fengycins (A) , obtained from a HPLC-purified peak after separation of CLP from B. amyloliquefaciens MEP 2 18, using nanoHPLC technology (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific). ESI-MS/MS spectra of protonated fengycin ions m/z 1463.8 (B) obtained from peak 1, the spectrum of precursor ion of m/z 1463.8 (C) ; m/z 1477.8 (D) obtained from peak 2, the spectrum of precursor ion of m/z 1477.8 (E) ; and ions m/z 1477.8, 1491.8, and 1505.8 (F) obtained from peak 3, the spectrum of precursor ion of m/z 1477.8 (G) , 1491.8 (H) , and 1505.8 (I) were acquired in a mass spectrometer (Thermo Scientific, Q-Exactive) with a high collision dissociation (HCD) cell and an Orbitrap analyzer.

    Article Snippet: Fengycins were purified with ZipTips C18 and analyzed by nanoHPLC (Thermo Scientific, EASY-nLC 1000) equipped with a reverse-phase C18 column (Easy-Spray ColumnPepMap RSLC, P/N ES803, 2 μm particle size, 75 μm × 500 mm, Thermo Scientific) at 35°C.

    Techniques: Tandem Mass Spectroscopy, High Performance Liquid Chromatography, Purification, Mass Spectrometry