easy nanolc 1000 chromatograph  (Thermo Fisher)


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    Name:
    EASY nLC 1000 Liquid Chromatograph
    Description:
    Upgrade to the Thermo Scientific EASY nLC 1200 Liquid Chromatography system The EASY nLC 1000 has been discontinued and has been replaced by the optimized EASY nLC 1200 system Service spare parts and consumables for the EASY nLC 1000 system are still available through the normal channels Please contact your local representative to discuss your system requirements
    Catalog Number:
    lc120
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher easy nanolc 1000 chromatograph
    Upgrade to the Thermo Scientific EASY nLC 1200 Liquid Chromatography system The EASY nLC 1000 has been discontinued and has been replaced by the optimized EASY nLC 1200 system Service spare parts and consumables for the EASY nLC 1000 system are still available through the normal channels Please contact your local representative to discuss your system requirements
    https://www.bioz.com/result/easy nanolc 1000 chromatograph/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    easy nanolc 1000 chromatograph - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Reversed-phase Chromatography:

    Article Title: Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes
    Article Snippet: .. In-gel digests were then desalted, fractionated online by reversed-phase chromatography using a Thermo Fisher easy-nLC 1000 liquid chromatography system, and analyzed by tandem mass spectrometry on a Thermo Fisher Q-Exactive mass spectrometer ( , ). .. Database searching was performed by using the MSGF+ search algorithm and a human protein database and considered differential modification of cysteine residues by carbamidomethylation (+57.021464 atomic mass units [amu]), 2-succination (+116.010959 amu), 2-monomethyl-succination (+130.026609 amu), and 2-dimethyl-succination (+144.042259 amu) ( ).

    Flow Cytometry:

    Article Title: Spliceosome profiling visualizes operations of a dynamic RNP at nucleotide resolution
    Article Snippet: .. Samples were separated at a flow rate of 400 nL/min on a Thermo Easy-nLC 1000 (Thermo Fisher Scientific). .. Samples were injected directly onto a 30 cm, 75 μm ID column packed with BEH 1.7 μm C18 resin (Waters).

    Liquid Chromatography:

    Article Title: Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes
    Article Snippet: .. In-gel digests were then desalted, fractionated online by reversed-phase chromatography using a Thermo Fisher easy-nLC 1000 liquid chromatography system, and analyzed by tandem mass spectrometry on a Thermo Fisher Q-Exactive mass spectrometer ( , ). .. Database searching was performed by using the MSGF+ search algorithm and a human protein database and considered differential modification of cysteine residues by carbamidomethylation (+57.021464 atomic mass units [amu]), 2-succination (+116.010959 amu), 2-monomethyl-succination (+130.026609 amu), and 2-dimethyl-succination (+144.042259 amu) ( ).

    Article Title: LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores
    Article Snippet: .. LC-MS/MS analyses were performed on an Easy-nLC 1000 liquid chromatography system (Thermo) coupled to a LTQ-Orbitrap Fusion (Thermo) via a nano-electrospray ion source. .. For raw MS file from CENP-E ubiquitylation, raw file was searched against the human National Center for Biotechnology Information (NCBI) Refseq protein database in Proteome Discoverer 2.1 suited with Mascot software (version 2.3.1, Matrix Science) to achieve a false discovery rate of < 1%.

    Article Title: A multipass membrane protein interacts with the cGMP-dependent protein kinase to regulate critical calcium signals in malaria parasites
    Article Snippet: .. LC-ESI-MS/MS was performed either on a Q-Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) equipped with an Easy nLC 1000 liquid chromatography system (Thermo Fisher Scientific) or an Orbitrap Fusion Lumos Tribrid mass Spectrometer (Thermo Fisher Scientific) equipped with an Easy nLC 1200 liquid chromatography system (Thermo Fisher Scientific). .. Peptides were trapped on an Acclaim pepmap100, 3 μm C18, 75 μm × 20 mm nano trap-column (Thermo Fisher Scientific) and separated on a 75 μm × 250 mm (Q-Exactive) or 500 mm (Orbitrap Fusion Lumos), 2 μm C18, 100 Å Easy-Spray column (Thermo Fisher Scientific).

    Article Title: Proteases Underground: Analysis of the Maize Root Apoplast Identifies Organ Specific Papain-Like Cysteine Protease Activity
    Article Snippet: .. LC/MS/MS Experiments were performed on an Orbitrap Elite instrument (Thermo Fischer Scientific, Waltham, MA, United States; ) that was coupled to an EASY-nLC 1000 liquid chromatography (LC) system (Thermo Fischer Scientific, Waltham, MA, United States). ..

    Mass Spectrometry:

    Article Title: Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes
    Article Snippet: .. In-gel digests were then desalted, fractionated online by reversed-phase chromatography using a Thermo Fisher easy-nLC 1000 liquid chromatography system, and analyzed by tandem mass spectrometry on a Thermo Fisher Q-Exactive mass spectrometer ( , ). .. Database searching was performed by using the MSGF+ search algorithm and a human protein database and considered differential modification of cysteine residues by carbamidomethylation (+57.021464 atomic mass units [amu]), 2-succination (+116.010959 amu), 2-monomethyl-succination (+130.026609 amu), and 2-dimethyl-succination (+144.042259 amu) ( ).

    Article Title: Ancestral reconstruction reveals mechanisms of ERK regulatory evolution
    Article Snippet: .. Mass spectrometry analysis 1 µg of each sample was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific) with solvent A consisting of 2% acetonitrile in 0.5% acetic acid and solvent B consisting of 80% acetonitrile in 0.5% acetic acid. .. The peptides were eluted into either an Orbitrap QExactive (Thermo Fisher Scientific) or Orbitrap QExactive HF-X mass spectrometer (Thermo Fisher Scientific) respectively, using the following gradient: 5–35% in 60 min, 36–45% in 10 min, followed by 45–100% in 10 min.

    Article Title: A multipass membrane protein interacts with the cGMP-dependent protein kinase to regulate critical calcium signals in malaria parasites
    Article Snippet: .. LC-ESI-MS/MS was performed either on a Q-Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) equipped with an Easy nLC 1000 liquid chromatography system (Thermo Fisher Scientific) or an Orbitrap Fusion Lumos Tribrid mass Spectrometer (Thermo Fisher Scientific) equipped with an Easy nLC 1200 liquid chromatography system (Thermo Fisher Scientific). .. Peptides were trapped on an Acclaim pepmap100, 3 μm C18, 75 μm × 20 mm nano trap-column (Thermo Fisher Scientific) and separated on a 75 μm × 250 mm (Q-Exactive) or 500 mm (Orbitrap Fusion Lumos), 2 μm C18, 100 Å Easy-Spray column (Thermo Fisher Scientific).

    Article Title: Cylindromatosis Tumor Suppressor Protein (CYLD) Deubiquitinase is Necessary for Proper Ubiquitination and Degradation of the Epidermal Growth Factor Receptor *
    Article Snippet: .. Peptides were separated by reversed-phase in an EASY-nLC 1000 (Thermo Scientific) coupled to a mass spectrometer equipped with a nanoelectrospray ion source. .. The samples were analyzed in either an LTQ Orbitrap Velos or a Q Exactive (Thermo Scientific).

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  • 99
    Thermo Fisher easy nanolc 1000 liquid chromatograph
    Easy Nanolc 1000 Liquid Chromatograph, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher easy nanolc 1000
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