eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two <t>Eag</t> I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eag i hf/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    eag i hf - by Bioz Stars, 2022-08
    93/100 stars

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    1) Product Images from "Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate"

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.02568

    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Figure Legend Snippet: Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Techniques Used: Produced

    PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.
    Figure Legend Snippet: PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Techniques Used: Marker

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    New England Biolabs eag i hf
    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two <t>Eag</t> I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eag i hf/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eag i hf - by Bioz Stars, 2022-08
    93/100 stars
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    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Journal: Frontiers in Microbiology

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    doi: 10.3389/fmicb.2019.02568

    Figure Lengend Snippet: Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Article Snippet: To determine the physical map of pELF1, the excised plugs containing pELF1 DNAs were separately digested at 37°C for 24 h with 20 U of Sal I-HF (New England BioLabs, Ipswich, MA, United States), at 25°C for 24 h with 100 U of Sma I (New England BioLabs), at 37°C for 24 h with 150 U of Eag I-HF (New England BioLabs), and at 37°C for 24 h with 150 U of Sac I-HF (New England BioLabs).

    Techniques: Produced

    PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Journal: Frontiers in Microbiology

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    doi: 10.3389/fmicb.2019.02568

    Figure Lengend Snippet: PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Article Snippet: To determine the physical map of pELF1, the excised plugs containing pELF1 DNAs were separately digested at 37°C for 24 h with 20 U of Sal I-HF (New England BioLabs, Ipswich, MA, United States), at 25°C for 24 h with 100 U of Sma I (New England BioLabs), at 37°C for 24 h with 150 U of Eag I-HF (New England BioLabs), and at 37°C for 24 h with 150 U of Sac I-HF (New England BioLabs).

    Techniques: Marker