eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig . (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and <t>two</t> <t>Eag</t> <t>I</t> sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate"

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.02568

    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig . (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Figure Legend Snippet: Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig . (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Techniques Used: Produced

    PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.
    Figure Legend Snippet: PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Techniques Used: Marker

    eag i hf  (New England Biolabs)


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    New England Biolabs eag i hf
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs eag i hf
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