human hybrid cell line ea hy 926  (ATCC)


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    ATCC human hybrid cell line ea hy 926
    Thrombin inhibitor, Argatroban didn’t inhibit C4a-induced ERK phosphorylation at the concentration that can inhibit thrombin-induced Ak-de-phosphorylation in Ea.hy 926 human ECs. A . Argatroban (4, 12, and 40 nM) reverses thrombin-induced Akt de-phosphorylation. B . Argatroban (12 nM and 40 nM) has no effects on C4a-induced ERK phosphorylation. Each experiment has been done at least three times with the same tendency.
    Human Hybrid Cell Line Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Divergent effects of C4a, C4a desArg , and thrombin on platelet aggregation and phosphorylation of ERK and Akt in human endothelial cells"

    Article Title: Divergent effects of C4a, C4a desArg , and thrombin on platelet aggregation and phosphorylation of ERK and Akt in human endothelial cells

    Journal: bioRxiv

    doi: 10.1101/2024.03.13.584877

    Thrombin inhibitor, Argatroban didn’t inhibit C4a-induced ERK phosphorylation at the concentration that can inhibit thrombin-induced Ak-de-phosphorylation in Ea.hy 926 human ECs. A . Argatroban (4, 12, and 40 nM) reverses thrombin-induced Akt de-phosphorylation. B . Argatroban (12 nM and 40 nM) has no effects on C4a-induced ERK phosphorylation. Each experiment has been done at least three times with the same tendency.
    Figure Legend Snippet: Thrombin inhibitor, Argatroban didn’t inhibit C4a-induced ERK phosphorylation at the concentration that can inhibit thrombin-induced Ak-de-phosphorylation in Ea.hy 926 human ECs. A . Argatroban (4, 12, and 40 nM) reverses thrombin-induced Akt de-phosphorylation. B . Argatroban (12 nM and 40 nM) has no effects on C4a-induced ERK phosphorylation. Each experiment has been done at least three times with the same tendency.

    Techniques Used: Concentration Assay, De-Phosphorylation Assay

    ea hy 926  (ATCC)


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    ATCC ea hy 926
    4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.
    Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Stress-induced stenotic vascular remodeling via reduction of plasma omega-3 fatty acid metabolite 4-oxoDHA by noradrenaline"

    Article Title: Stress-induced stenotic vascular remodeling via reduction of plasma omega-3 fatty acid metabolite 4-oxoDHA by noradrenaline

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-54867-3

    4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.
    Figure Legend Snippet: 4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.

    Techniques Used: Sequencing, Western Blot, Expressing, Incubation, Permeability, Labeling, Quantitative RT-PCR

    ea hy 926 cell line  (ATCC)


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    ATCC ea hy 926 cell line
    Ea Hy 926 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    endothelial cells ea hy 926 cells  (ATCC)


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    ATCC endothelial cells ea hy 926 cells
    Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.
    Endothelial Cells Ea Hy 926 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation"

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    Journal: Journal of Cancer

    doi: 10.7150/jca.91566

    Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.
    Figure Legend Snippet: Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.

    Techniques Used: Migration

    Effects of NDF on expression of EndoMT markers in EA.hy 926 cells. Expressions of VE-Cadherin (panel A) and α-SMA (panel B) were analyzed by immunofluorometric assay (×200). Panel C and panel D showed electrophoretic bands and results of densitometric analysis obtained from western blotting assay. **p<0.01 compared with TGF-β1; ##p<0.01 compared with CTRL; &p<0.05 compared with NDF(5μM).
    Figure Legend Snippet: Effects of NDF on expression of EndoMT markers in EA.hy 926 cells. Expressions of VE-Cadherin (panel A) and α-SMA (panel B) were analyzed by immunofluorometric assay (×200). Panel C and panel D showed electrophoretic bands and results of densitometric analysis obtained from western blotting assay. **p<0.01 compared with TGF-β1; ##p<0.01 compared with CTRL; &p<0.05 compared with NDF(5μM).

    Techniques Used: Expressing, Western Blot

    Effects of NDF on the Ezrin in TGF-β1-treated EA.hy 926 cells. A: the effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin axis activities by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with TGF-β1; &p<0.05 and &&p<0.01 compared with NDF(5μM). Lane 1: control; lane 2: TGF-β1; lane 3: NDF (5μM); lane 4: NDF (20μM).
    Figure Legend Snippet: Effects of NDF on the Ezrin in TGF-β1-treated EA.hy 926 cells. A: the effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin axis activities by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with TGF-β1; &p<0.05 and &&p<0.01 compared with NDF(5μM). Lane 1: control; lane 2: TGF-β1; lane 3: NDF (5μM); lane 4: NDF (20μM).

    Techniques Used: Expressing, Immunoprecipitation

    Effects of NDF on angiogenesis induced by VEGF. A: Effects of NDF on tube formation of EA.hy 926; B: Effects of NDF on rat aortic ring angiogenesis. C: Effects of NDF on expression of several angiogenic markers in EA.hy 926 cells. **p<0.01 compared with VEGF group; ##p<0.01 compared with CTRL, &&p<0.01 compared with NDF(5μM).
    Figure Legend Snippet: Effects of NDF on angiogenesis induced by VEGF. A: Effects of NDF on tube formation of EA.hy 926; B: Effects of NDF on rat aortic ring angiogenesis. C: Effects of NDF on expression of several angiogenic markers in EA.hy 926 cells. **p<0.01 compared with VEGF group; ##p<0.01 compared with CTRL, &&p<0.01 compared with NDF(5μM).

    Techniques Used: Expressing

    Effects of NDF on Ezrin in VEGF-treated EA.hy 926 cells. A: Effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with VEGF. Lane 1: control; lane 2: VEGF; lane 3: NDF (5μM); lane 4: NDF (20μM).
    Figure Legend Snippet: Effects of NDF on Ezrin in VEGF-treated EA.hy 926 cells. A: Effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with VEGF. Lane 1: control; lane 2: VEGF; lane 3: NDF (5μM); lane 4: NDF (20μM).

    Techniques Used: Expressing, Immunoprecipitation

    Effects of NDF on TGF-β1-induced EndoMT and VEGF-induced angiogenesis when Ezrin was knocked-down in EA.hy 926 cells. Effects of NDF on invasion (A) and expression of EndoMT markers (B, C and D) in Ezrin-knocking-down EA.hy 926 cells at presence of TGF-β1; Effects of NDF on tube formation (E and F) , expression of angiogenic markers (G, H and I) in Ezrin-knocking-down EA.hy 926 cells at presence of VEGF. ##p<0.01 compared with CTRL; *p<0.05 and **p<0.01 compared with siRNA.
    Figure Legend Snippet: Effects of NDF on TGF-β1-induced EndoMT and VEGF-induced angiogenesis when Ezrin was knocked-down in EA.hy 926 cells. Effects of NDF on invasion (A) and expression of EndoMT markers (B, C and D) in Ezrin-knocking-down EA.hy 926 cells at presence of TGF-β1; Effects of NDF on tube formation (E and F) , expression of angiogenic markers (G, H and I) in Ezrin-knocking-down EA.hy 926 cells at presence of VEGF. ##p<0.01 compared with CTRL; *p<0.05 and **p<0.01 compared with siRNA.

    Techniques Used: Expressing

    ea hy 926  (ATCC)


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    ATCC ea hy 926
    (a) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. (b) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6h at 37°C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean±s.e. (n=3). **** indicates p<0.0001. (c) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6h at 37°C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean±s.e. (n=5). ** indicates p<0.01. (d) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2h at 37°C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean±s.e. (n=3) **, *** indicate p<0.01, p<0.001. (e) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1h, followed by stimulation of H 2 O 2 (500 µM) for 2h at 37°C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean±s.e. (n=3–4). ** indicates p<0.01. (f, g) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 (f) and E-selectin (g) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean±s.e. (n=7). *, ** indicate p<0.05, p<0.01.
    Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ea hy 926 - by Bioz Stars, 2024-04
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    1) Product Images from "Stress-induced vascular remodeling: novel insight into the role of omega-3 fatty acid metabolite, 4-oxoDHA"

    Article Title: Stress-induced vascular remodeling: novel insight into the role of omega-3 fatty acid metabolite, 4-oxoDHA

    Journal: bioRxiv

    doi: 10.1101/2023.07.25.550603

    (a) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. (b) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6h at 37°C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean±s.e. (n=3). **** indicates p<0.0001. (c) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6h at 37°C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean±s.e. (n=5). ** indicates p<0.01. (d) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2h at 37°C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean±s.e. (n=3) **, *** indicate p<0.01, p<0.001. (e) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1h, followed by stimulation of H 2 O 2 (500 µM) for 2h at 37°C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean±s.e. (n=3–4). ** indicates p<0.01. (f, g) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 (f) and E-selectin (g) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean±s.e. (n=7). *, ** indicate p<0.05, p<0.01.
    Figure Legend Snippet: (a) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. (b) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6h at 37°C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean±s.e. (n=3). **** indicates p<0.0001. (c) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6h at 37°C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean±s.e. (n=5). ** indicates p<0.01. (d) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2h at 37°C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean±s.e. (n=3) **, *** indicate p<0.01, p<0.001. (e) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1h, followed by stimulation of H 2 O 2 (500 µM) for 2h at 37°C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean±s.e. (n=3–4). ** indicates p<0.01. (f, g) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 (f) and E-selectin (g) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean±s.e. (n=7). *, ** indicate p<0.05, p<0.01.

    Techniques Used: Sequencing, Western Blot, Expressing, Incubation, Permeability, Labeling, Quantitative RT-PCR

    human endothelial cells ea hy 926  (ATCC)


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    ATCC human endothelial cells ea hy 926
    Human Endothelial Cells Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human endothelial cells ea hy 926  (ATCC)


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    ATCC human endothelial cells ea hy 926
    Human Endothelial Cells Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human endothelial cells ea hy 926  (ATCC)


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    ATCC human endothelial cells ea hy 926
    Characterization of human endothelial cells EA.hy 926. Immunocytochemical staining (A + B) and in-vitro-angiogenesis assay (C + D). Cells showed a strong expression of CD-31 ( A ) and vWF ( B ). After being seeded on extracellular matrix solution ( C ) they formed a fine network of tubules within 18 h ( D ). Scale bar represents 100 μm
    Human Endothelial Cells Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat"

    Article Title: Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-022-00767-1

    Characterization of human endothelial cells EA.hy 926. Immunocytochemical staining (A + B) and in-vitro-angiogenesis assay (C + D). Cells showed a strong expression of CD-31 ( A ) and vWF ( B ). After being seeded on extracellular matrix solution ( C ) they formed a fine network of tubules within 18 h ( D ). Scale bar represents 100 μm
    Figure Legend Snippet: Characterization of human endothelial cells EA.hy 926. Immunocytochemical staining (A + B) and in-vitro-angiogenesis assay (C + D). Cells showed a strong expression of CD-31 ( A ) and vWF ( B ). After being seeded on extracellular matrix solution ( C ) they formed a fine network of tubules within 18 h ( D ). Scale bar represents 100 μm

    Techniques Used: Staining, In Vitro, Angiogenesis Assay, Expressing

    Representative microscopic images after 5 days of rat kidney scaffold recellularization with human endothelial cells EA.hy 926. The left column displays multiple attached endothelial cells covering the vascular basement membrane as a monolayer after 0.66% SDS while after 3% SDS there was only a small number of detectable cells (right column). Evaluation of recellularization success was primarily obtained with HE-staining ( A , B ). Staining for CD-31 ( C , D ) confirms preservation of endothelial phenotype and staining for PCNA ( E , F ) indicates proliferative behavior. Apoptotic cells were detected by ISNT ( G , H , arrows). Scale bar represents 100 μm. N = 3 for each column
    Figure Legend Snippet: Representative microscopic images after 5 days of rat kidney scaffold recellularization with human endothelial cells EA.hy 926. The left column displays multiple attached endothelial cells covering the vascular basement membrane as a monolayer after 0.66% SDS while after 3% SDS there was only a small number of detectable cells (right column). Evaluation of recellularization success was primarily obtained with HE-staining ( A , B ). Staining for CD-31 ( C , D ) confirms preservation of endothelial phenotype and staining for PCNA ( E , F ) indicates proliferative behavior. Apoptotic cells were detected by ISNT ( G , H , arrows). Scale bar represents 100 μm. N = 3 for each column

    Techniques Used: Staining, Preserving

    Semiquantitative analysis of relative cell count after 5 days of dynamic culture with endothelial cells EA.hy 926. For cell counting, 20 microscopic images in 200 times magnification from 4 different section levels of each kidney were used. Representative images are shown in A for 3%SDS and B for 0.66% SDS, respectively. Arrows indicate adherent cells. For improved comparison of recellularization procedures, the relative quantity of adherent cells was then calculated by setting the counted cells in relation to 1 × 10 6 inserted cells. The mean (± SD) of the relative cell count was 0.62 (± 0.07) and 1.39 (± 0.26) after decellularization at 3% SDS and 0.66% SDS, respectively ( p = 0.1, two-tailed Mann–Whitney U test). Relative cell count could be more than doubled after using the gentle decellularization protocol at 0.66% SDS (4C). n = 3 for each SDS concentration
    Figure Legend Snippet: Semiquantitative analysis of relative cell count after 5 days of dynamic culture with endothelial cells EA.hy 926. For cell counting, 20 microscopic images in 200 times magnification from 4 different section levels of each kidney were used. Representative images are shown in A for 3%SDS and B for 0.66% SDS, respectively. Arrows indicate adherent cells. For improved comparison of recellularization procedures, the relative quantity of adherent cells was then calculated by setting the counted cells in relation to 1 × 10 6 inserted cells. The mean (± SD) of the relative cell count was 0.62 (± 0.07) and 1.39 (± 0.26) after decellularization at 3% SDS and 0.66% SDS, respectively ( p = 0.1, two-tailed Mann–Whitney U test). Relative cell count could be more than doubled after using the gentle decellularization protocol at 0.66% SDS (4C). n = 3 for each SDS concentration

    Techniques Used: Cell Counting, Two Tailed Test, MANN-WHITNEY, Concentration Assay

    endothelial ea hy 926  (ATCC)


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    ATCC endothelial ea hy 926
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    endothelial ea hy 926  (ATCC)


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    ATCC human hybrid cell line ea hy 926
    Thrombin inhibitor, Argatroban didn’t inhibit C4a-induced ERK phosphorylation at the concentration that can inhibit thrombin-induced Ak-de-phosphorylation in Ea.hy 926 human ECs. A . Argatroban (4, 12, and 40 nM) reverses thrombin-induced Akt de-phosphorylation. B . Argatroban (12 nM and 40 nM) has no effects on C4a-induced ERK phosphorylation. Each experiment has been done at least three times with the same tendency.
    Human Hybrid Cell Line Ea Hy 926, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ea hy 926
    4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.
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    ATCC ea hy 926 cell line
    4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.
    Ea Hy 926 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC endothelial cells ea hy 926 cells
    Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.
    Endothelial Cells Ea Hy 926 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human endothelial cells ea hy 926
    Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.
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    ATCC endothelial ea hy 926
    Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.
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    Thrombin inhibitor, Argatroban didn’t inhibit C4a-induced ERK phosphorylation at the concentration that can inhibit thrombin-induced Ak-de-phosphorylation in Ea.hy 926 human ECs. A . Argatroban (4, 12, and 40 nM) reverses thrombin-induced Akt de-phosphorylation. B . Argatroban (12 nM and 40 nM) has no effects on C4a-induced ERK phosphorylation. Each experiment has been done at least three times with the same tendency.

    Journal: bioRxiv

    Article Title: Divergent effects of C4a, C4a desArg , and thrombin on platelet aggregation and phosphorylation of ERK and Akt in human endothelial cells

    doi: 10.1101/2024.03.13.584877

    Figure Lengend Snippet: Thrombin inhibitor, Argatroban didn’t inhibit C4a-induced ERK phosphorylation at the concentration that can inhibit thrombin-induced Ak-de-phosphorylation in Ea.hy 926 human ECs. A . Argatroban (4, 12, and 40 nM) reverses thrombin-induced Akt de-phosphorylation. B . Argatroban (12 nM and 40 nM) has no effects on C4a-induced ERK phosphorylation. Each experiment has been done at least three times with the same tendency.

    Article Snippet: The human hybrid cell line EA.hy 926 (ATCC), made via fusion of HUVEC with A549 lung adenocarcinoma cells, was maintained in DMEM with 10% conditioned FBS, penicillin (100 units/mL), streptomycin (100 mg/mL), and L-glutamine (2 mM).

    Techniques: Concentration Assay, De-Phosphorylation Assay

    4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.

    Journal: Scientific Reports

    Article Title: Stress-induced stenotic vascular remodeling via reduction of plasma omega-3 fatty acid metabolite 4-oxoDHA by noradrenaline

    doi: 10.1038/s41598-024-54867-3

    Figure Lengend Snippet: 4-oxoDHA augments Nrf2-HO-1 pathways and anti-inflammatory properties in endothelial cells. ( a ) Pathway analysis of RNA-sequence data from the control (N/C) and 4-oxoDHA treated (4-oxoDHA) HUVECs. ( b ) HUVECs were treated with DHA (10 µM), 4-HDHA (0.1–10 µM), and 4-oxoDHA (0.1–10 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine heme oxygenase-1 (HO-1) expression. β-actin was employed as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 3). ****Indicates p < 0.0001. ( c ) Endothelial cell line (EA.hy 926) was treated with DHA (1 µM), 4-HDHA (0.01–1 µM), and 4-oxoDHA (0.01–1 µM) for 6 h at 37 °C. Whole-cell lysates were processed for western blot analysis to determine HO-1 expression. β-actin was used as the internal standard. Data are expressed as fold induction of HO-1 compared to control, mean ± s.e. (n = 5). **Indicates p < 0.01. ( d ) HUVECs were treated with 10 µM of DHA, 4-HDHA, and 4-oxoDHA for 2 h at 37 °C. Nuclear fractions were processed for western blot analysis to determine Nrf2 expression. PCNA was used as the internal standard. Data are expressed as fold induction of Nrf2 compared to control (N/C), mean ± s.e. (n = 3) **,***Indicate p < 0.01, p < 0.001. ( e ) EA.hy 926 cells were treated with 1 µM of DHA, 4-HDHA, and 4-oxoDHA for 1 h, followed by stimulation of H 2 O 2 (500 µM) for 2 h at 37 °C. After incubation, endothelial permeability was investigated with FITC-labeled dextran (MW: 10,000). Data are expressed as percent reduction compared to control, mean ± s.e. (n = 3–4). **Indicates p < 0.01. ( f,g ) HUVECs were treated with 10 µM DHA or 4-oxoDHA after stimulation with Kdo2 (0.5 µg/mL). ICAM-1 ( f ) and E-selectin ( g ) mRNA were analyzed using real-time RT-PCR. Data are expressed as fold change compared to control, mean ± s.e. (n = 7). *,**Indicate p < 0.05, p < 0.01.

    Article Snippet: RAW 264.7, HL-60, EA.hy 926, and HUVEC were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), Riken BRC (BioResource Research Center, JP), and Lonza (Basel, Switzerland).

    Techniques: Sequencing, Western Blot, Expressing, Incubation, Permeability, Labeling, Quantitative RT-PCR

    Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.

    Journal: Journal of Cancer

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    doi: 10.7150/jca.91566

    Figure Lengend Snippet: Effects of NDF on TGF-β1-induced cell motility. A: Effects of NDF on cell migration (×100); B: numbers of migrated cells; C: Effects of NDF on cell invasion (×40); D: Effects of NDF on F-actin assembly in EA.hy 926 cells (×400 and ×630). *p<0.05 and **p<0.01 compared with TGF-β1; #p<0.05 compared with CTRL.

    Article Snippet: Another human endothelial cells EA.hy 926 cells, obtained from American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 1% pen/strep in 5% CO 2 at 37 °C.

    Techniques: Migration

    Effects of NDF on expression of EndoMT markers in EA.hy 926 cells. Expressions of VE-Cadherin (panel A) and α-SMA (panel B) were analyzed by immunofluorometric assay (×200). Panel C and panel D showed electrophoretic bands and results of densitometric analysis obtained from western blotting assay. **p<0.01 compared with TGF-β1; ##p<0.01 compared with CTRL; &p<0.05 compared with NDF(5μM).

    Journal: Journal of Cancer

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    doi: 10.7150/jca.91566

    Figure Lengend Snippet: Effects of NDF on expression of EndoMT markers in EA.hy 926 cells. Expressions of VE-Cadherin (panel A) and α-SMA (panel B) were analyzed by immunofluorometric assay (×200). Panel C and panel D showed electrophoretic bands and results of densitometric analysis obtained from western blotting assay. **p<0.01 compared with TGF-β1; ##p<0.01 compared with CTRL; &p<0.05 compared with NDF(5μM).

    Article Snippet: Another human endothelial cells EA.hy 926 cells, obtained from American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 1% pen/strep in 5% CO 2 at 37 °C.

    Techniques: Expressing, Western Blot

    Effects of NDF on the Ezrin in TGF-β1-treated EA.hy 926 cells. A: the effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin axis activities by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with TGF-β1; &p<0.05 and &&p<0.01 compared with NDF(5μM). Lane 1: control; lane 2: TGF-β1; lane 3: NDF (5μM); lane 4: NDF (20μM).

    Journal: Journal of Cancer

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    doi: 10.7150/jca.91566

    Figure Lengend Snippet: Effects of NDF on the Ezrin in TGF-β1-treated EA.hy 926 cells. A: the effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin axis activities by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with TGF-β1; &p<0.05 and &&p<0.01 compared with NDF(5μM). Lane 1: control; lane 2: TGF-β1; lane 3: NDF (5μM); lane 4: NDF (20μM).

    Article Snippet: Another human endothelial cells EA.hy 926 cells, obtained from American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 1% pen/strep in 5% CO 2 at 37 °C.

    Techniques: Expressing, Immunoprecipitation

    Effects of NDF on angiogenesis induced by VEGF. A: Effects of NDF on tube formation of EA.hy 926; B: Effects of NDF on rat aortic ring angiogenesis. C: Effects of NDF on expression of several angiogenic markers in EA.hy 926 cells. **p<0.01 compared with VEGF group; ##p<0.01 compared with CTRL, &&p<0.01 compared with NDF(5μM).

    Journal: Journal of Cancer

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    doi: 10.7150/jca.91566

    Figure Lengend Snippet: Effects of NDF on angiogenesis induced by VEGF. A: Effects of NDF on tube formation of EA.hy 926; B: Effects of NDF on rat aortic ring angiogenesis. C: Effects of NDF on expression of several angiogenic markers in EA.hy 926 cells. **p<0.01 compared with VEGF group; ##p<0.01 compared with CTRL, &&p<0.01 compared with NDF(5μM).

    Article Snippet: Another human endothelial cells EA.hy 926 cells, obtained from American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 1% pen/strep in 5% CO 2 at 37 °C.

    Techniques: Expressing

    Effects of NDF on Ezrin in VEGF-treated EA.hy 926 cells. A: Effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with VEGF. Lane 1: control; lane 2: VEGF; lane 3: NDF (5μM); lane 4: NDF (20μM).

    Journal: Journal of Cancer

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    doi: 10.7150/jca.91566

    Figure Lengend Snippet: Effects of NDF on Ezrin in VEGF-treated EA.hy 926 cells. A: Effects of NDF on expression of Ezrin and p-Ezrin; B: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-Ezrin antibody was used as sedimental protein; C: Effects of NDF on PODXL-Ezrin interaction by immunoprecipitation while anti-PODXL antibody was used as sedimental protein. *p<0.05 and **p<0.01 compared with control; #p<0.05 and ##p<0.01 compared with VEGF. Lane 1: control; lane 2: VEGF; lane 3: NDF (5μM); lane 4: NDF (20μM).

    Article Snippet: Another human endothelial cells EA.hy 926 cells, obtained from American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 1% pen/strep in 5% CO 2 at 37 °C.

    Techniques: Expressing, Immunoprecipitation

    Effects of NDF on TGF-β1-induced EndoMT and VEGF-induced angiogenesis when Ezrin was knocked-down in EA.hy 926 cells. Effects of NDF on invasion (A) and expression of EndoMT markers (B, C and D) in Ezrin-knocking-down EA.hy 926 cells at presence of TGF-β1; Effects of NDF on tube formation (E and F) , expression of angiogenic markers (G, H and I) in Ezrin-knocking-down EA.hy 926 cells at presence of VEGF. ##p<0.01 compared with CTRL; *p<0.05 and **p<0.01 compared with siRNA.

    Journal: Journal of Cancer

    Article Title: Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation

    doi: 10.7150/jca.91566

    Figure Lengend Snippet: Effects of NDF on TGF-β1-induced EndoMT and VEGF-induced angiogenesis when Ezrin was knocked-down in EA.hy 926 cells. Effects of NDF on invasion (A) and expression of EndoMT markers (B, C and D) in Ezrin-knocking-down EA.hy 926 cells at presence of TGF-β1; Effects of NDF on tube formation (E and F) , expression of angiogenic markers (G, H and I) in Ezrin-knocking-down EA.hy 926 cells at presence of VEGF. ##p<0.01 compared with CTRL; *p<0.05 and **p<0.01 compared with siRNA.

    Article Snippet: Another human endothelial cells EA.hy 926 cells, obtained from American Type Culture Collection (Manassas, VA, USA), were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 1% pen/strep in 5% CO 2 at 37 °C.

    Techniques: Expressing