e7420  (New England Biolabs)


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  • 99
    Name:
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7420l
    Price:
    3877
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    Structured Review

    New England Biolabs e7420
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/e7420/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e7420 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Transcriptomic dataset reveals the molecular basis of genotypic variation in hexaploid wheat (T. aestivum L.) in response to Fe/Zn deficiency
    Article Snippet: .. Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol. ..

    Article Title: Whole genome screen reveals a novel relationship between Wolbachia levels and Drosophila host translation
    Article Snippet: .. After rRNA depletion libraries were prepared according to manufacturer’s instructions using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, E7420L) and NEBNext Multiplex Oligos for Illumina Index Primers Set I (Illumina, E7335). .. After adaptor ligation, the libraries were amplified by qPCR using the KAPA Real-time amplification kit (KAPA Biosystems).

    RNA Sequencing Assay:

    Article Title: Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs
    Article Snippet: .. An NEBNext ultradirectional RNA library kit (New England Biolabs, catalog no. E7420S) was used to make cDNA and RNA-seq libraries. .. Several minor modifications to the standard kit protocol were made based on (1) size (RNA fragmentation time was decreased from 15 to 5 min) and (2) the small amount of RNA (the number of PCR cycles were adjusted to 20 cycles in the USER excision and PCR library step).

    Construct:

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner
    Article Snippet: .. One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S). .. Briefly, poly(A)+ RNA was purified with oligo-d(T) beads, and fragmented to ~200 nucleotide lengths prior to cDNA synthesis using random primers.

    Purification:

    Article Title: Transcriptomic dataset reveals the molecular basis of genotypic variation in hexaploid wheat (T. aestivum L.) in response to Fe/Zn deficiency
    Article Snippet: .. Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol. ..

    Generated:

    Article Title: A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species
    Article Snippet: .. RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com ) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany. .. RNAseq analysis Illumina 100 bp paired-end reads from four biological replicates of the four internode zones were imported into CLC genomics workbench 8 (CLCbio, Aarhus, Denmark, http://www.clcbio.com ) for adapter removal, trimming, mapping, and read counting.

    other:

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification
    Article Snippet: Input sample libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on an Illumina HiSeq2000 at the Tayside Centre for Genomics Analysis, University of Dundee, with a pair-end read length of 75 bp.

    Sequencing:

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner
    Article Snippet: .. One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S). .. Briefly, poly(A)+ RNA was purified with oligo-d(T) beads, and fragmented to ~200 nucleotide lengths prior to cDNA synthesis using random primers.

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  • 99
    New England Biolabs ultra directional rna library prep kit
    Analysis of the F. novicida greA locus. ( A ) Alignment of four Gram-negative bacterial GreA amino acid sequences using the MegAlign program of the DNAstar Lasergene package (version 10). Identical amino acid residues are shown in black. The cross-link with the <t>RNA</t> 3′-terminus is shown in a red box and the conserved acidic residues (D43 and E46) required for GreA activity are shown in green boxes. ( B ) Schematic illustration of the gene arrangement at the greA locus. ( C ) Cotranscription of the greA locus genes determined with RT–PCR. The pepA-–guaB (a), guaB– FTN_0662 (b), FTN_0662 –pgi (c), pgi–fimT (d), fimT–greA (e), and greA–uvrA (f) junctions were amplified using DNA, DNA-free RNA, or <t>cDNA</t> as the template. The images were acquired by the gel imaging system (LIUYI, Beijing, China). The experiment was repeated twice. The sizes of the molecular markers are indicated at the side in kbp.
    Ultra Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra directional rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 1112 article reviews
    Price from $9.99 to $1999.99
    ultra directional rna library prep kit - by Bioz Stars, 2020-09
    99/100 stars
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    Analysis of the F. novicida greA locus. ( A ) Alignment of four Gram-negative bacterial GreA amino acid sequences using the MegAlign program of the DNAstar Lasergene package (version 10). Identical amino acid residues are shown in black. The cross-link with the RNA 3′-terminus is shown in a red box and the conserved acidic residues (D43 and E46) required for GreA activity are shown in green boxes. ( B ) Schematic illustration of the gene arrangement at the greA locus. ( C ) Cotranscription of the greA locus genes determined with RT–PCR. The pepA-–guaB (a), guaB– FTN_0662 (b), FTN_0662 –pgi (c), pgi–fimT (d), fimT–greA (e), and greA–uvrA (f) junctions were amplified using DNA, DNA-free RNA, or cDNA as the template. The images were acquired by the gel imaging system (LIUYI, Beijing, China). The experiment was repeated twice. The sizes of the molecular markers are indicated at the side in kbp.

    Journal: Scientific Reports

    Article Title: Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida

    doi: 10.1038/s41598-018-25271-5

    Figure Lengend Snippet: Analysis of the F. novicida greA locus. ( A ) Alignment of four Gram-negative bacterial GreA amino acid sequences using the MegAlign program of the DNAstar Lasergene package (version 10). Identical amino acid residues are shown in black. The cross-link with the RNA 3′-terminus is shown in a red box and the conserved acidic residues (D43 and E46) required for GreA activity are shown in green boxes. ( B ) Schematic illustration of the gene arrangement at the greA locus. ( C ) Cotranscription of the greA locus genes determined with RT–PCR. The pepA-–guaB (a), guaB– FTN_0662 (b), FTN_0662 –pgi (c), pgi–fimT (d), fimT–greA (e), and greA–uvrA (f) junctions were amplified using DNA, DNA-free RNA, or cDNA as the template. The images were acquired by the gel imaging system (LIUYI, Beijing, China). The experiment was repeated twice. The sizes of the molecular markers are indicated at the side in kbp.

    Article Snippet: The Ribo-Zero™ Magnetic Kit (Epicentre, Madison, WI, USA) was used to remove the rRNA, and the rRNA-depleted RNA was used to generate cDNA libraries with the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB).

    Techniques: Activity Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Imaging

    Verification of RNA-seq data. ( A ) Detection of gene transcription in the wild-type U112 strain and the Δ greA mutant. Transcription levels of genes by RNA-seq were shown with solid bars. Relative level of each target gene (open bars) by qRT-PCR was normalized to that of the 16S rRNA gene. Data are presented as mean fold changes relative to the wild-type U112 strain ± SD of the results from triplicate samples. The experiment was repeated twice. ( B ) Detection of protein expression in the wild-type U112 strain and the Δ greA mutant. Mid-log bacteria were resuspended in PBS to OD 600 = 1.0. The suspensions were concentrated 10-fold, separated with SDS-PAGE, and detected with western blotting, using antiserum specific for each target protein. The left panel is a section of a coomassie stained gel as a loading control. The coomassie stained gel image was acquired with the digital camera (Canon, Janpan). The experiment was repeated twice. Size of each protein is indicated on the left in kDa.

    Journal: Scientific Reports

    Article Title: Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida

    doi: 10.1038/s41598-018-25271-5

    Figure Lengend Snippet: Verification of RNA-seq data. ( A ) Detection of gene transcription in the wild-type U112 strain and the Δ greA mutant. Transcription levels of genes by RNA-seq were shown with solid bars. Relative level of each target gene (open bars) by qRT-PCR was normalized to that of the 16S rRNA gene. Data are presented as mean fold changes relative to the wild-type U112 strain ± SD of the results from triplicate samples. The experiment was repeated twice. ( B ) Detection of protein expression in the wild-type U112 strain and the Δ greA mutant. Mid-log bacteria were resuspended in PBS to OD 600 = 1.0. The suspensions were concentrated 10-fold, separated with SDS-PAGE, and detected with western blotting, using antiserum specific for each target protein. The left panel is a section of a coomassie stained gel as a loading control. The coomassie stained gel image was acquired with the digital camera (Canon, Janpan). The experiment was repeated twice. Size of each protein is indicated on the left in kDa.

    Article Snippet: The Ribo-Zero™ Magnetic Kit (Epicentre, Madison, WI, USA) was used to remove the rRNA, and the rRNA-depleted RNA was used to generate cDNA libraries with the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB).

    Techniques: RNA Sequencing Assay, Mutagenesis, Quantitative RT-PCR, Expressing, SDS Page, Western Blot, Staining

    Schematic work flow of library preparation and sequencing using NEBNext Ultra Directional RNA Library Preparation kit.

    Journal: Data in Brief

    Article Title: Transcriptomic dataset reveals the molecular basis of genotypic variation in hexaploid wheat (T. aestivum L.) in response to Fe/Zn deficiency

    doi: 10.1016/j.dib.2020.105995

    Figure Lengend Snippet: Schematic work flow of library preparation and sequencing using NEBNext Ultra Directional RNA Library Preparation kit.

    Article Snippet: Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol.

    Techniques: Sequencing

    RNA-seq analysis of BioTAP-XL pull-downs. ( A ) Enrichment of repeat-derived RNA in HP1a-BioTAP cross-linked complexes from S2 cells compared with MSL3-BioTAP complexes from S2 cells detected using a random-priming approach for cDNA synthesis and Illumina

    Journal: Genes & Development

    Article Title: Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs

    doi: 10.1101/gad.241950.114

    Figure Lengend Snippet: RNA-seq analysis of BioTAP-XL pull-downs. ( A ) Enrichment of repeat-derived RNA in HP1a-BioTAP cross-linked complexes from S2 cells compared with MSL3-BioTAP complexes from S2 cells detected using a random-priming approach for cDNA synthesis and Illumina

    Article Snippet: An NEBNext ultradirectional RNA library kit (New England Biolabs, catalog no. E7420S) was used to make cDNA and RNA-seq libraries.

    Techniques: RNA Sequencing Assay, Derivative Assay

    Internode zones have unique RNAseq profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from RNA sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )

    Journal: Biotechnology for Biofuels

    Article Title: A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species

    doi: 10.1186/s13068-016-0457-6

    Figure Lengend Snippet: Internode zones have unique RNAseq profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from RNA sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )

    Article Snippet: RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com ) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany.

    Techniques: RNA Sequencing Assay