Structured Review

Genewiz e1b mutation
Construction and characterization of the <t>E1B</t> 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair
E1b Mutation, supplied by Genewiz, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
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e1b mutation - by Bioz Stars, 2020-09
88/100 stars

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1) Product Images from "Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53"

Article Title: Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53

Journal: Journal of Virology

doi: 10.1128/JVI.03072-14

Construction and characterization of the E1B 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair
Figure Legend Snippet: Construction and characterization of the E1B 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair

Techniques Used: Mutagenesis

Related Articles

Mutagenesis:

Article Title: Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53
Article Snippet: .. To assay for the E4 mutation, the region of the genome between bp 34353 and 34382 was amplified by PCR of viral DNAs containing the E1B mutation and sequenced (Genewiz). .. Once the presence of both mutations was confirmed, the mutant was subjected to an additional cycle of plaque purification and verification and was then propagated in 293 cells infected at 1 PFU/cell and maintained at 39°C for 44 h.

Polymerase Chain Reaction:

Article Title: Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53
Article Snippet: .. To assay for the E4 mutation, the region of the genome between bp 34353 and 34382 was amplified by PCR of viral DNAs containing the E1B mutation and sequenced (Genewiz). .. Once the presence of both mutations was confirmed, the mutant was subjected to an additional cycle of plaque purification and verification and was then propagated in 293 cells infected at 1 PFU/cell and maintained at 39°C for 44 h.

Amplification:

Article Title: Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53
Article Snippet: .. To assay for the E4 mutation, the region of the genome between bp 34353 and 34382 was amplified by PCR of viral DNAs containing the E1B mutation and sequenced (Genewiz). .. Once the presence of both mutations was confirmed, the mutant was subjected to an additional cycle of plaque purification and verification and was then propagated in 293 cells infected at 1 PFU/cell and maintained at 39°C for 44 h.

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    Genewiz e1b mutation
    Construction and characterization of the <t>E1B</t> 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair
    E1b Mutation, supplied by Genewiz, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e1b mutation/product/Genewiz
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e1b mutation - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    Construction and characterization of the E1B 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair

    Journal: Journal of Virology

    Article Title: Impact of the Adenoviral E4 Orf3 Protein on the Activity and Posttranslational Modification of p53

    doi: 10.1128/JVI.03072-14

    Figure Lengend Snippet: Construction and characterization of the E1B 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair

    Article Snippet: To assay for the E4 mutation, the region of the genome between bp 34353 and 34382 was amplified by PCR of viral DNAs containing the E1B mutation and sequenced (Genewiz).

    Techniques: Mutagenesis