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Proteintech rabbit polyclonal anti e1b ap5
MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and <t>E1B-AP5</t> antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.
Rabbit Polyclonal Anti E1b Ap5, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti e1b ap5/product/Proteintech
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti e1b ap5 - by Bioz Stars, 2020-09
99/100 stars

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1) Product Images from "Stability of a Long Noncoding Viral RNA Depends on a 9-nt Core Element at the RNA 5' End to Interact with Viral ORF57 and Cellular PABPC1"

Article Title: Stability of a Long Noncoding Viral RNA Depends on a 9-nt Core Element at the RNA 5' End to Interact with Viral ORF57 and Cellular PABPC1

Journal: International Journal of Biological Sciences

doi:

MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and E1B-AP5 antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.
Figure Legend Snippet: MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and E1B-AP5 antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.

Techniques Used: In Vitro, Binding Assay, Sequencing, Derivative Assay, Positive Control, Western Blot, Plasmid Preparation

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    Proteintech rabbit polyclonal anti e1b ap5
    MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and <t>E1B-AP5</t> antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.
    Rabbit Polyclonal Anti E1b Ap5, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti e1b ap5/product/Proteintech
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti e1b ap5 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Proteintech e1b ap5 polyclonal antibody
    MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and <t>E1B-AP5</t> antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.
    E1b Ap5 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e1b ap5 polyclonal antibody/product/Proteintech
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e1b ap5 polyclonal antibody - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and E1B-AP5 antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.

    Journal: International Journal of Biological Sciences

    Article Title: Stability of a Long Noncoding Viral RNA Depends on a 9-nt Core Element at the RNA 5' End to Interact with Viral ORF57 and Cellular PABPC1

    doi:

    Figure Lengend Snippet: MRE-II RNA interacts in vitro with ORF57 and other cellular proteins. ( A ) Mapping of an MRE binding site for ORF57. Shown on the top is a PAN MRE sequence lacking the MRE-I region in Fig. 4 A, with the 9-nt core of MRE-II loop sequence bolded and underlined and the nt positions of biotinylated RNA oligomers employed in RNA pull-down assays. A cell lysate prepared from TREx BCBL-1-Rta cells 39 induced with Dox for 24 h was used for the RNA pulldown assays with an indicated RNA oligomer. The RNA oligo oNP42 derived from vIL-6 RNA 27 which binds ORF57 was used as a positive control. ORF57 associated with RNA oligos in the pulldowns was immunoblotted using an anti-ORF57 antibody. The cell lysate (10%) before the pulldown was loaded as a Western blot control. ( B ) Biotinylated RNA affinity pulldown analysis by Western blot using anti-ORF57, PABPC1 and E1B-AP5 antibodies. Total cell extract from TREx BCBL-1-Rta (R) or -vector (V) cells induced by Dox for 24 h was used for the RNA pulldown assays with each biotinylated RNA oligomer. RNA oligos oNP41 and oNP42 derived from vIL-6 RNA 27 were used, respectively, as a negative and positive oligomer control for ORF57 interaction and 10% of the cell lysate from each cell line before the pulldown was loaded for Western blotting control. RNA oligo oJM68 is a copy of oJM35 with the point mutations in the MRE-II loop described in Fig. 4 B. Control beads indicate no RNA oligomer on the beads.

    Article Snippet: The following antibodies were used in Western blot analyses: a rabbit polyclonal anti-ORF57 antibody (1:3,000 dilution), mouse monoclonal anti-ORF57 antibody (unpublished data, used at a dilution of 1:1,000), rabbit polyclonal anti-PABPC1 (1:700, Abcam ab21060), and rabbit polyclonal anti-E1B-AP5 (1:1,000, ProteinTech Group 0578-1-AP, Chicago, IL), together with corresponding peroxidase-conjugated secondary antibodies (1:10,000, Sigma).

    Techniques: In Vitro, Binding Assay, Sequencing, Derivative Assay, Positive Control, Western Blot, Plasmid Preparation