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Oncogene Science Inc e1b 19 kda
HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in <t>kDa.</t> ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and <t>E1B-19-kDa.</t> HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.
E1b 19 Kda, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins"

Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in kDa. ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.
Figure Legend Snippet: HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in kDa. ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.

Techniques Used: Transformation Assay, Expressing, Immunoprecipitation, Labeling, Transfection, Plasmid Preparation, Infection, Marker, Southern Blot, Software

The IE1 and IE2 gene products are expressed transiently during the transformation of BRK cells. BRK cells seeded on coverslips were transfected with expression plasmids for E1A and E1B-19-kDa ( Left ) or plasmids for E1A and IE1 and IE2 ( Right ). At 2, 5, 13, and 19 days after transfection, two coverslips from each transfection were fixed and kept in phosphate-buffered saline until coverslips for all time points were collected. Expression of the transfected genes was examined by indirect immunofluorescent staining with appropriate monoclonal antibodies. Anti-E1A, anti-E1B-19-kDa, and anti-IE1/2 identify the antibodies used for the immunofluorescent staining. Images are from independent coverslips. The images from days 13 and 19 display transformed foci whose cells were positive for E1A and E1B-19-kDa proteins, but completely negative for IE1 and IE2 protein expression. (×50).
Figure Legend Snippet: The IE1 and IE2 gene products are expressed transiently during the transformation of BRK cells. BRK cells seeded on coverslips were transfected with expression plasmids for E1A and E1B-19-kDa ( Left ) or plasmids for E1A and IE1 and IE2 ( Right ). At 2, 5, 13, and 19 days after transfection, two coverslips from each transfection were fixed and kept in phosphate-buffered saline until coverslips for all time points were collected. Expression of the transfected genes was examined by indirect immunofluorescent staining with appropriate monoclonal antibodies. Anti-E1A, anti-E1B-19-kDa, and anti-IE1/2 identify the antibodies used for the immunofluorescent staining. Images are from independent coverslips. The images from days 13 and 19 display transformed foci whose cells were positive for E1A and E1B-19-kDa proteins, but completely negative for IE1 and IE2 protein expression. (×50).

Techniques Used: Transformation Assay, Transfection, Expressing, Staining

Related Articles

Staining:

Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins
Article Snippet: .. For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science). .. Coverslips with cells transfected with vectors expressing E1A, IE1, and IE2 proteins were allowed to react with either anti-E1A (M73) or anti-HCMV IE1/2 (mAB810) antibody.

Transfection:

Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins
Article Snippet: .. For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science). .. Coverslips with cells transfected with vectors expressing E1A, IE1, and IE2 proteins were allowed to react with either anti-E1A (M73) or anti-HCMV IE1/2 (mAB810) antibody.

Expressing:

Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins
Article Snippet: .. For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science). .. Coverslips with cells transfected with vectors expressing E1A, IE1, and IE2 proteins were allowed to react with either anti-E1A (M73) or anti-HCMV IE1/2 (mAB810) antibody.

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    Oncogene Science Inc e1b 19 kda
    HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in <t>kDa.</t> ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and <t>E1B-19-kDa.</t> HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.
    E1b 19 Kda, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e1b 19 kda/product/Oncogene Science Inc
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    80
    Oncogene Science Inc e1b 19 kda proteins
    HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in <t>kDa.</t> ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and <t>E1B-19-kDa.</t> HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.
    E1b 19 Kda Proteins, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e1b 19 kda proteins/product/Oncogene Science Inc
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e1b 19 kda proteins - by Bioz Stars, 2020-09
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    HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in kDa. ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins

    doi:

    Figure Lengend Snippet: HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in kDa. ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.

    Article Snippet: For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science).

    Techniques: Transformation Assay, Expressing, Immunoprecipitation, Labeling, Transfection, Plasmid Preparation, Infection, Marker, Southern Blot, Software

    The IE1 and IE2 gene products are expressed transiently during the transformation of BRK cells. BRK cells seeded on coverslips were transfected with expression plasmids for E1A and E1B-19-kDa ( Left ) or plasmids for E1A and IE1 and IE2 ( Right ). At 2, 5, 13, and 19 days after transfection, two coverslips from each transfection were fixed and kept in phosphate-buffered saline until coverslips for all time points were collected. Expression of the transfected genes was examined by indirect immunofluorescent staining with appropriate monoclonal antibodies. Anti-E1A, anti-E1B-19-kDa, and anti-IE1/2 identify the antibodies used for the immunofluorescent staining. Images are from independent coverslips. The images from days 13 and 19 display transformed foci whose cells were positive for E1A and E1B-19-kDa proteins, but completely negative for IE1 and IE2 protein expression. (×50).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins

    doi:

    Figure Lengend Snippet: The IE1 and IE2 gene products are expressed transiently during the transformation of BRK cells. BRK cells seeded on coverslips were transfected with expression plasmids for E1A and E1B-19-kDa ( Left ) or plasmids for E1A and IE1 and IE2 ( Right ). At 2, 5, 13, and 19 days after transfection, two coverslips from each transfection were fixed and kept in phosphate-buffered saline until coverslips for all time points were collected. Expression of the transfected genes was examined by indirect immunofluorescent staining with appropriate monoclonal antibodies. Anti-E1A, anti-E1B-19-kDa, and anti-IE1/2 identify the antibodies used for the immunofluorescent staining. Images are from independent coverslips. The images from days 13 and 19 display transformed foci whose cells were positive for E1A and E1B-19-kDa proteins, but completely negative for IE1 and IE2 protein expression. (×50).

    Article Snippet: For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science).

    Techniques: Transformation Assay, Transfection, Expressing, Staining

    HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in kDa. ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins

    doi:

    Figure Lengend Snippet: HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. ( A ) Lack of expression of the IE1 or IE2 protein in transformed BRK cell lines when assayed by immunoprecipitation of 35 S-labeled cell extracts using monoclonal antibody mAB810. A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2–1 to A/1/2–10 are cell lines transformed by E1A plus IE1 and IE2. Lanes designated HCMV INF display immunoprecipitates from HCMV-infected human foreskin fibroblasts; lane 13 is an exposure 1/8 as long as that presented for lanes 1–12. Numbers to the left of the gel mark the positions of marker proteins whose sizes are indicated in kDa. ( B ) Southern blot analysis showing the lack of IE1 - or IE2 -specific DNA in E1A/IE1/IE2-induced transformants (lanes 1–10). A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing IE1) are included as controls. pCGN-IE1 and pCGN-IE2 are IE1 and IE2 expression plasmids, respectively, in amounts equivalent to the rest of the samples, assuming they contain one copy of each of the HCMV genes per cell. Numbers to the left of the gel are marker DNA sizes in kb. ( C ) Expression of E1A protein as determined by immunoprecipitation followed by immunoblot analysis, both with monoclonal antibody M73 to E1A. Lanes are labeled as in A and B . Numbers to the left of the gel are marker protein sizes in kDa. Autoradiograms were scanned and cropped using Photoshop and figures were prepared using Freehand software.

    Article Snippet: For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science).

    Techniques: Transformation Assay, Expressing, Immunoprecipitation, Labeling, Transfection, Plasmid Preparation, Infection, Marker, Southern Blot, Software

    The IE1 and IE2 gene products are expressed transiently during the transformation of BRK cells. BRK cells seeded on coverslips were transfected with expression plasmids for E1A and E1B-19-kDa ( Left ) or plasmids for E1A and IE1 and IE2 ( Right ). At 2, 5, 13, and 19 days after transfection, two coverslips from each transfection were fixed and kept in phosphate-buffered saline until coverslips for all time points were collected. Expression of the transfected genes was examined by indirect immunofluorescent staining with appropriate monoclonal antibodies. Anti-E1A, anti-E1B-19-kDa, and anti-IE1/2 identify the antibodies used for the immunofluorescent staining. Images are from independent coverslips. The images from days 13 and 19 display transformed foci whose cells were positive for E1A and E1B-19-kDa proteins, but completely negative for IE1 and IE2 protein expression. (×50).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins

    doi:

    Figure Lengend Snippet: The IE1 and IE2 gene products are expressed transiently during the transformation of BRK cells. BRK cells seeded on coverslips were transfected with expression plasmids for E1A and E1B-19-kDa ( Left ) or plasmids for E1A and IE1 and IE2 ( Right ). At 2, 5, 13, and 19 days after transfection, two coverslips from each transfection were fixed and kept in phosphate-buffered saline until coverslips for all time points were collected. Expression of the transfected genes was examined by indirect immunofluorescent staining with appropriate monoclonal antibodies. Anti-E1A, anti-E1B-19-kDa, and anti-IE1/2 identify the antibodies used for the immunofluorescent staining. Images are from independent coverslips. The images from days 13 and 19 display transformed foci whose cells were positive for E1A and E1B-19-kDa proteins, but completely negative for IE1 and IE2 protein expression. (×50).

    Article Snippet: For the two sets of coverslips collected from cells transfected with vectors expressing the E1A plus E1B-19-kDa proteins, one set was stained with anti-E1A antibody (M73), and the other was stained with monoclonal antibody to E1B-19-kDa (Oncogene Science).

    Techniques: Transformation Assay, Transfection, Expressing, Staining