Journal: FASEB BioAdvances

Article Title: Mapping Intestinal Paracellular Perm Eability in Mice: Regional and Cellular Variability Under Physiological and Stimulated Conditions

doi: 10.1096/fba.2025-00325

Figure Lengend Snippet: Localization of FM distribution in jejunal villi (A) and colon (B) using confocal microscopy. FM dye appears green, WGA appears cyan in the glycoproteins of plasma membranes and mucus on the surface or within GC cells, and the nucleus is stained blue with DAPI. FM is visible on the apical surface of the intestinal epithelium, but has also penetrated around certain epithelial cells, including GC. The GC appears either full (white arrows) or depleted of mucus (cyan arrows). Cr = crypt as indicated with orange arrows, E = enterocytes, L = lumen, LP = lamina propria. Bars 50 μm.

Article Snippet: After three washings of 5 min, the sections were finally mounted with fluorescent mounting medium with DAPI (4‐6 diamino‐2‐phenylindole, Origen E1918).

Techniques: Confocal Microscopy, Clinical Proteomics, Staining

Journal: FASEB BioAdvances

Article Title: Mapping Intestinal Paracellular Perm Eability in Mice: Regional and Cellular Variability Under Physiological and Stimulated Conditions

doi: 10.1096/fba.2025-00325

Figure Lengend Snippet: Immunodetection was used to identify permeable epithelial cells in the jejunum or colon that were stained by the FM dye (green). This revealed cell expressing GC mucin 2 (A), chromogranin A‐containing enteroendocrine cells (B), DCAMLCK1‐containing tuft cells (C) and caspase 3‐expressing apoptotic cells (D), which were stained red. The mucin 2 and the WGA colocalized in the tissue surface and into CG (A). Bars = 20 μm. FM (green), WGA (cyan), immunodetection (red) and nucleus (DAPI blue). Bars = 20 μm.

Article Snippet: After three washings of 5 min, the sections were finally mounted with fluorescent mounting medium with DAPI (4‐6 diamino‐2‐phenylindole, Origen E1918).

Techniques: Immunodetection, Staining, Expressing

Journal: FASEB BioAdvances

Article Title: Mapping Intestinal Paracellular Perm Eability in Mice: Regional and Cellular Variability Under Physiological and Stimulated Conditions

doi: 10.1096/fba.2025-00325

Figure Lengend Snippet: Effect of chronic psychological stress in corticoid treated mice (CORT) versus controls (CTRL). Compared to controls, CORT mice showed significative differences either in weight gain (A), increased in permeability in vivo (B) or ex vivo in the colon (C). The confocal imaging of FM uptake in the jejunum (Jej, D for CTRL, E for CORT) does not shown increased permeability whereas such increase is clearly visible in the CORT (G) versus CTRL (F) colon. FM (green) and DAPI (blue). Cr = crypt, E = enterocytes, L = lumen, LP = lamina propria. Bars 50 μm. Significative differences: *** p < 0.002; ** p < 0.02.

Article Snippet: After three washings of 5 min, the sections were finally mounted with fluorescent mounting medium with DAPI (4‐6 diamino‐2‐phenylindole, Origen E1918).

Techniques: Permeability, In Vivo, Ex Vivo, Imaging

Journal: FASEB BioAdvances

Article Title: Mapping Intestinal Paracellular Perm Eability in Mice: Regional and Cellular Variability Under Physiological and Stimulated Conditions

doi: 10.1096/fba.2025-00325

Figure Lengend Snippet: Detection of the uptake FM and lipids in the jejunum after gavage with control solution lipid free (A, D), or emulsions containing olive (B, E) or palm oil (D, F). (A–C) Staining with FM (green), WGA (magenta) and DAPI (blue) show an important uptake of the FM around enterocytes showing increased permeability after olive or palm oil nutrition. In control mice, the villus is devoid of lipid droplets (D), while gavage with olive (E) or palm oil (F) show the presence of numerous lipid droplets stained with LD540 (red), mainly in enterocytes. DAPI stained nucleus in blue. L = lumen, LP = lamina propria. Bars 50 μm.

Article Snippet: After three washings of 5 min, the sections were finally mounted with fluorescent mounting medium with DAPI (4‐6 diamino‐2‐phenylindole, Origen E1918).

Techniques: Control, Staining, Permeability

Journal: FASEB BioAdvances

Article Title: Mapping Intestinal Paracellular Perm Eability in Mice: Regional and Cellular Variability Under Physiological and Stimulated Conditions

doi: 10.1096/fba.2025-00325

Figure Lengend Snippet: Confocal detection of the uptake of FM (green) or lipids in the jejunum 3.5 h after ingestion of conventional kibble (6% fat W/W) (A–C) or kibble enriched to 46% fat (D–F). Low fat diet provides no increase in permeability (B) compared to controls (A) and moderate content of lipid droplets (E) labeled with LD540 in red. Fat enriched kibble diet induces moderate increase in permeability across enterocytes (C) and shows the presence of lipid droplets (F). WGA (magenta) DAPI (blue). L = lumen, LP = lamina propria. Bars 50 μm.

Article Snippet: After three washings of 5 min, the sections were finally mounted with fluorescent mounting medium with DAPI (4‐6 diamino‐2‐phenylindole, Origen E1918).

Techniques: Permeability, Labeling