Structured Review

Epitomics e155
Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and <t>E155.</t> These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
E155, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e155/product/Epitomics
Average 92 stars, based on 23 article reviews
Price from $9.99 to $1999.99
e155 - by Bioz Stars, 2022-10
92/100 stars

Images

1) Product Images from "Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac"

Article Title: Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac

Journal: Cell reports

doi: 10.1016/j.celrep.2020.02.012

Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
Figure Legend Snippet: Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

Techniques Used: Western Blot, Expressing

2) Product Images from "Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac"

Article Title: Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac

Journal: Cell reports

doi: 10.1016/j.celrep.2020.02.012

Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
Figure Legend Snippet: Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

Techniques Used: Western Blot, Expressing

3) Product Images from "Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac"

Article Title: Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac

Journal: Cell reports

doi: 10.1016/j.celrep.2020.02.012

Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
Figure Legend Snippet: Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

Techniques Used: Western Blot, Expressing

4) Product Images from "Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac"

Article Title: Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac

Journal: Cell reports

doi: 10.1016/j.celrep.2020.02.012

Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
Figure Legend Snippet: Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

Techniques Used: Western Blot, Expressing

5) Product Images from "Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac"

Article Title: Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac

Journal: Cell reports

doi: 10.1016/j.celrep.2020.02.012

Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
Figure Legend Snippet: Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

Techniques Used: Western Blot, Expressing

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    Epitomics e155
    Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and <t>E155.</t> These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .
    E155, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e155/product/Epitomics
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e155 - by Bioz Stars, 2022-10
    92/100 stars
      Buy from Supplier

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    Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

    Journal: Cell reports

    Article Title: Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac

    doi: 10.1016/j.celrep.2020.02.012

    Figure Lengend Snippet: Signal from Phospho-Tyr 307 Antibodies Is Insensitive to Alkaline Phosphatase Treatment (A-F) Western blot validation of control or alkaline phosphatase (AP)-treated H358 cells expressing either LacZ or wtPP2Ac probed with (A) Pan phospho Tyrosine, (B) phospho-EGFR, and (C) phospho-SRC. Western blotsfor phospho-Tyr 307 PP2Ac antibodiesfrom (D) Abcam, (E) R D Systems, and (F) Santa Cruz for control or AP-treated H358 cells expressing either LacZ or wtPP2Ac. Images are representative of three independent experiments. The pan-phospho-tyrosine blot was stripped and cut at appropriate molecularweights and reprobed with phospho-SRC and E155. These three share the same Vinculin loading control (A, C, and D). A second blot was run, treated with AP, and cut for phospho-EGFR and R D. These two share the same vinculin loading control (B and E). A separate blot was run and treated for Santa Cruz. .

    Article Snippet: The image is representative of 4 (E155 and F-8) or 6 (R & D) independent experiments.

    Techniques: Western Blot, Expressing