Review



sphk1  (Echelon Biosciences)


Bioz Verified Symbol Echelon Biosciences is a verified supplier
Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Echelon Biosciences sphk1
    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of <t>SphK1</t> and SphK2. ( A , B ): ATP concentrations (µM) were measured by the <t>Sphingosine</t> <t>Kinase</t> Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).
    Sphk1, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk1/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    sphk1 - by Bioz Stars, 2025-02
    90/100 stars

    Images

    1) Product Images from "Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents"

    Article Title: Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms18112332

    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).
    Figure Legend Snippet: Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).

    Techniques Used: Kinase Assay, Enzyme Inhibition Assay

    Comparison of the molecular models of inhibitors 5h and 8f . The outline of the “J” site, typical of the SphK1 isoform active site is superimposed to both models. In the case of the 8f model, the outline is prolonged to show the good complementarity with the shape of the active site.
    Figure Legend Snippet: Comparison of the molecular models of inhibitors 5h and 8f . The outline of the “J” site, typical of the SphK1 isoform active site is superimposed to both models. In the case of the 8f model, the outline is prolonged to show the good complementarity with the shape of the active site.

    Techniques Used:



    Similar Products

    sphk1  (Echelon Biosciences)
    90
    Echelon Biosciences sphk1
    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of <t>SphK1</t> and SphK2. ( A , B ): ATP concentrations (µM) were measured by the <t>Sphingosine</t> <t>Kinase</t> Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).
    Sphk1, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk1/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    sphk1 - by Bioz Stars, 2025-02
    90/100 stars
    		  Buy from Supplier
    e-k068  (Echelon Biosciences)
    90
    Echelon Biosciences e-k068
    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of <t>SphK1</t> and SphK2. ( A , B ): ATP concentrations (µM) were measured by the <t>Sphingosine</t> <t>Kinase</t> Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).
    E K068, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e-k068/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    e-k068 - by Bioz Stars, 2025-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).

    Journal: International Journal of Molecular Sciences

    Article Title: Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents

    doi: 10.3390/ijms18112332

    Figure Lengend Snippet: Effect of the tested compounds on the adenosine triphosphate (ATP) levels in the presence of SphK1 and SphK2. ( A , B ): ATP concentrations (µM) were measured by the Sphingosine Kinase Activity Assay (Echelon Biosciences) in the presence of SphK1 ( A ) and SphK2 ( B ), and tested with PF-543, 5h , and 8f . All the compounds were evaluated at the concentrations of 10 µM. For further details, see the Materials and Methods section. Data represent the mean (±SD) of two experiments performed in triplicate. Bars show SDs. ( C , D ): Percent of enzyme inhibition by PF-543, 5h , and 8f on SphK1 ( C ) and by 5h on SphK2 ( D ), respectively, as measured by the Sphingosine Kinase Activity Assay ( Echelon Biosciences ).

    Article Snippet: SphK1 (E-K068, Echelon Biosciences) and SphK2 (E-K069, Echelon Biosciences) were added at a concentration of 500 ng/mL.

    Techniques: Kinase Assay, Enzyme Inhibition Assay

    Comparison of the molecular models of inhibitors 5h and 8f . The outline of the “J” site, typical of the SphK1 isoform active site is superimposed to both models. In the case of the 8f model, the outline is prolonged to show the good complementarity with the shape of the active site.

    Journal: International Journal of Molecular Sciences

    Article Title: Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents

    doi: 10.3390/ijms18112332

    Figure Lengend Snippet: Comparison of the molecular models of inhibitors 5h and 8f . The outline of the “J” site, typical of the SphK1 isoform active site is superimposed to both models. In the case of the 8f model, the outline is prolonged to show the good complementarity with the shape of the active site.

    Article Snippet: SphK1 (E-K068, Echelon Biosciences) and SphK2 (E-K069, Echelon Biosciences) were added at a concentration of 500 ng/mL.

    Techniques: