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alomone tamapin  (Alomone Labs)
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a-b Representative ventral root recordings showing the effect of the broad-spectrum SK <t>activator</t> <t>1-EBIO</t> ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor <t>tamapin</t> ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.
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a-b Representative ventral root recordings showing the effect of the broad-spectrum SK <t>activator</t> <t>1-EBIO</t> ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor <t>tamapin</t> ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.
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growth conditions 150 s cerevisiae atcc 13007  (ATCC)
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a-b Representative ventral root recordings showing the effect of the broad-spectrum SK <t>activator</t> <t>1-EBIO</t> ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor <t>tamapin</t> ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.
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a-b Representative ventral root recordings showing the effect of the broad-spectrum SK <t>activator</t> <t>1-EBIO</t> ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor <t>tamapin</t> ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.
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filter  (Sartorius AG)
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a-b Representative ventral root recordings showing the effect of the broad-spectrum SK <t>activator</t> <t>1-EBIO</t> ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor <t>tamapin</t> ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.
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e coli atcc 35 150  (ATCC)
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ATCC e coli atcc 35 150
a-b Representative ventral root recordings showing the effect of the broad-spectrum SK <t>activator</t> <t>1-EBIO</t> ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor <t>tamapin</t> ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.
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Image Search Results


a-b Representative ventral root recordings showing the effect of the broad-spectrum SK activator 1-EBIO ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor tamapin ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.

Journal: bioRxiv

Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

doi: 10.64898/2026.03.19.712770

Figure Lengend Snippet: a-b Representative ventral root recordings showing the effect of the broad-spectrum SK activator 1-EBIO ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor tamapin ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.

Article Snippet: Alomone: tamapin (5-10 nM), 1-EBIO (200 μM), CyPPA (1-10 μM), and Tram-34 (5 μM).

Techniques: Isolation, Activity Assay