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    Name:
    Enterococcus faecium NCTC 7171 DSM 20477 JCM 8727 NCDO 942
    Description:

    Catalog Number:
    19434
    Price:
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    Applications:
    Testing antimicrobial handwashing formulations
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    Structured Review

    ATCC e faecium strains
    E. <t>faecium</t> in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P

    https://www.bioz.com/result/e faecium strains/product/ATCC
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    1) Product Images from "Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host"

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    Journal: Genome Biology

    doi: 10.1186/s13059-019-1879-9

    E. faecium in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P
    Figure Legend Snippet: E. faecium in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P

    Techniques Used: Mouse Assay, Transplantation Assay, Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    The presence of E. faecium is associated with disease extent and the requirement for combination therapy. The disease characteristics and treatment regimens of the 16 UC patients enrolled in this study were obtained from medical records and assessed to identify any association with the presence or absence of E. faecium in the gut microbiota as determined by PCR. a Proportions of UC patients with pancolitis or left-sided colitis are shown relative to the presence of E. faecium in the feces. b Proportions of UC patients treated with single or multiple mediations are shown relative to the presence of E. faecium in the feces. Medications included mesalazine, corticosteroids, azathioprine, mercaptopurine, tacrolimus, infliximab, and adalimumab. The number of subjects per category ( n ) is indicated. * P
    Figure Legend Snippet: The presence of E. faecium is associated with disease extent and the requirement for combination therapy. The disease characteristics and treatment regimens of the 16 UC patients enrolled in this study were obtained from medical records and assessed to identify any association with the presence or absence of E. faecium in the gut microbiota as determined by PCR. a Proportions of UC patients with pancolitis or left-sided colitis are shown relative to the presence of E. faecium in the feces. b Proportions of UC patients treated with single or multiple mediations are shown relative to the presence of E. faecium in the feces. Medications included mesalazine, corticosteroids, azathioprine, mercaptopurine, tacrolimus, infliximab, and adalimumab. The number of subjects per category ( n ) is indicated. * P

    Techniques Used: Polymerase Chain Reaction

    Subject-derived E. faecium strains lead to different pathology and cytokine expression profiles in the colon. Fecal suspensions from selected subjects (HD55 and IBD36) and E. faecium strain IB18a, IB51a, or HD26a suspensions were transplanted or inoculated into microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Mean pathology scores of mice from each treatment group. d mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. e , f Suspension of HD55 or IBD51 feces or strain IB51a was gavaged into germ-free Il10 −/− mice. e Mean pathology scores of mice from each treatment group. f mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a – f are the mean ± SE. Statistical differences between a value and the HD55 control were analyzed using the Kruskal-Wallis test followed by Dunn’s test. * P
    Figure Legend Snippet: Subject-derived E. faecium strains lead to different pathology and cytokine expression profiles in the colon. Fecal suspensions from selected subjects (HD55 and IBD36) and E. faecium strain IB18a, IB51a, or HD26a suspensions were transplanted or inoculated into microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Mean pathology scores of mice from each treatment group. d mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. e , f Suspension of HD55 or IBD51 feces or strain IB51a was gavaged into germ-free Il10 −/− mice. e Mean pathology scores of mice from each treatment group. f mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a – f are the mean ± SE. Statistical differences between a value and the HD55 control were analyzed using the Kruskal-Wallis test followed by Dunn’s test. * P

    Techniques Used: Derivative Assay, Expressing, Mouse Assay, Transplantation Assay, Real-time Polymerase Chain Reaction

    Genome analysis of 10 E. faecium strains reveals inflammatory and probiotic clusters. a Three (HD26a, HD50a, and HD59a) and 4 (IB6a, IB18a, IB44a, and IB50a) E. faecium strains were isolated from the feces of HD subjects and UC patients, respectively. The genotypes of 10 E. faecium strains, including the 3 HD-derived and 4 UC-derived strains, inflammatory strain ATCC 19434, and probiotic strains NCIMB 11181 and SF68, were examined by sequencing. All 1683 identified genes (except for those coding for hypothetical proteins) were used for hierarchical clustering analysis of the 10 E. faecium strains. b LDA was performed using LEfSe to identify significant differences in KEGG-based metabolic pathways in the genomes of the 10 strains to compare between the inflammatory cluster in which ATCC 19434 was included and the probiotic cluster in which NCIMB 11181 and SF68 were included. Differentially abundant pathways for which the corresponding LDA scores indicate P
    Figure Legend Snippet: Genome analysis of 10 E. faecium strains reveals inflammatory and probiotic clusters. a Three (HD26a, HD50a, and HD59a) and 4 (IB6a, IB18a, IB44a, and IB50a) E. faecium strains were isolated from the feces of HD subjects and UC patients, respectively. The genotypes of 10 E. faecium strains, including the 3 HD-derived and 4 UC-derived strains, inflammatory strain ATCC 19434, and probiotic strains NCIMB 11181 and SF68, were examined by sequencing. All 1683 identified genes (except for those coding for hypothetical proteins) were used for hierarchical clustering analysis of the 10 E. faecium strains. b LDA was performed using LEfSe to identify significant differences in KEGG-based metabolic pathways in the genomes of the 10 strains to compare between the inflammatory cluster in which ATCC 19434 was included and the probiotic cluster in which NCIMB 11181 and SF68 were included. Differentially abundant pathways for which the corresponding LDA scores indicate P

    Techniques Used: Isolation, Derivative Assay, Sequencing

    2) Product Images from "A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci"

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    Journal: Annals of Saudi Medicine

    doi: 10.5144/0256-4947.2018.200

    PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.
    Figure Legend Snippet: PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Techniques Used: Polymerase Chain Reaction, Generated

    PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    Figure Legend Snippet: PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    3) Product Images from "A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci"

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    Journal: Annals of Saudi Medicine

    doi: 10.5144/0256-4947.2018.200

    PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    Figure Legend Snippet: PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.
    Figure Legend Snippet: PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Techniques Used: Polymerase Chain Reaction, Generated

    Related Articles

    Positive Control:

    Article Title: Antimicrobial resistance of Enterococcus faecium strains isolated from commercial probiotic products used in cattle and swine strains isolated from commercial probiotic products used in cattle and swine
    Article Snippet: .. The DNA was isolated by suspending a single colony in nuclease-free water with 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, CA) and boiled for 10 min. An ATCC strain of E. faecium (ATCC 19434; American Type Culture Collection, Manassas, VA) was used as a positive control in the assay. ..

    Isolation:

    Article Title: Antimicrobial resistance of Enterococcus faecium strains isolated from commercial probiotic products used in cattle and swine strains isolated from commercial probiotic products used in cattle and swine
    Article Snippet: .. The DNA was isolated by suspending a single colony in nuclease-free water with 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, CA) and boiled for 10 min. An ATCC strain of E. faecium (ATCC 19434; American Type Culture Collection, Manassas, VA) was used as a positive control in the assay. ..

    other:

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci
    Article Snippet: These referenced bacterial strains were as follow: Bacteroides fragilis ATCC 25285, VSE strains (E. faecalis NCTC 77, E faecium NCIMB 2699) and VRE strains (E faecium ATCC 19434, E faecalis ATCC 29212, E faecalis ATCC 51299).

    Expressing:

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host
    Article Snippet: .. Unlike isolates derived from healthy donors, E. faecium isolates from the feces of UC patients, along with E. faecium strain ATCC 19434, promotes colitis and colonic cytokine expression. .. Inflammatory E. faecium strains, including ATCC 19434 and a UC-derived strain, cluster separately from commercially available probiotic strains based on whole-genome shotgun sequencing analysis.

    Binding Assay:

    Article Title: Rapid and Accurate Diagnosis of Human Intestinal Spirochetosis by Fluorescence In Situ Hybridization ▿
    Article Snippet: .. A clinical isolate of B. pilosicoli and the nearest phylogenetic neighbors at the probe binding site, Enterococcus faecium (ATCC 19434) and Spirochaeta halophila (DSM 10522), with two mismatches each, were used as positive and negative controls, respectively, and were included throughout the study. .. Furthermore, the probe was tested against other cultivable spirochetes, i.e., Borrelia garinii (tick isolate; R. Ackermann, University Hospital of Cologne, Cologne, Germany), the oral treponeme Treponema denticola (ATCC 33521), and Leptospira biflexa and Leptospira interrogans (provided by V. Sambri, Section of Microbiology, St. Orsola Hospital, University of Bologna, Bologna, Italy).

    Derivative Assay:

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host
    Article Snippet: .. Unlike isolates derived from healthy donors, E. faecium isolates from the feces of UC patients, along with E. faecium strain ATCC 19434, promotes colitis and colonic cytokine expression. .. Inflammatory E. faecium strains, including ATCC 19434 and a UC-derived strain, cluster separately from commercially available probiotic strains based on whole-genome shotgun sequencing analysis.

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host
    Article Snippet: .. Three strains derived from the feces of UC patients, IB51a, IB6a, and IB44a, were clustered with pro-inflammatory strain ATCC 19434, while two strains derived from the feces of HD subjects, HD26a and HD50a, were clustered with probiotic strains NCIMB 11181 and SF68, although HD-derived strain HD59a was grouped into the other cluster. .. IB18a, which induced a different cytokine expression profile from that of strain IB51a, was distant in the plot from IB51a and was much closer to the probiotic strains (Fig. a and Additional file : Figure S9).

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    ATCC e faecium strains
    E. <t>faecium</t> in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P
    E Faecium Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 89/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e faecium strains/product/ATCC
    Average 89 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    e faecium strains - by Bioz Stars, 2020-07
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    E. faecium in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P

    Journal: Genome Biology

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    doi: 10.1186/s13059-019-1879-9

    Figure Lengend Snippet: E. faecium in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P

    Article Snippet: The genotypes of 10 analyzed E. faecium strains were different and could be separated into two major clusters: one containing two probiotic strains and the other containing pro-inflammatory strain ATCC 19434 (Fig. a).

    Techniques: Mouse Assay, Transplantation Assay, Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    The presence of E. faecium is associated with disease extent and the requirement for combination therapy. The disease characteristics and treatment regimens of the 16 UC patients enrolled in this study were obtained from medical records and assessed to identify any association with the presence or absence of E. faecium in the gut microbiota as determined by PCR. a Proportions of UC patients with pancolitis or left-sided colitis are shown relative to the presence of E. faecium in the feces. b Proportions of UC patients treated with single or multiple mediations are shown relative to the presence of E. faecium in the feces. Medications included mesalazine, corticosteroids, azathioprine, mercaptopurine, tacrolimus, infliximab, and adalimumab. The number of subjects per category ( n ) is indicated. * P

    Journal: Genome Biology

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    doi: 10.1186/s13059-019-1879-9

    Figure Lengend Snippet: The presence of E. faecium is associated with disease extent and the requirement for combination therapy. The disease characteristics and treatment regimens of the 16 UC patients enrolled in this study were obtained from medical records and assessed to identify any association with the presence or absence of E. faecium in the gut microbiota as determined by PCR. a Proportions of UC patients with pancolitis or left-sided colitis are shown relative to the presence of E. faecium in the feces. b Proportions of UC patients treated with single or multiple mediations are shown relative to the presence of E. faecium in the feces. Medications included mesalazine, corticosteroids, azathioprine, mercaptopurine, tacrolimus, infliximab, and adalimumab. The number of subjects per category ( n ) is indicated. * P

    Article Snippet: The genotypes of 10 analyzed E. faecium strains were different and could be separated into two major clusters: one containing two probiotic strains and the other containing pro-inflammatory strain ATCC 19434 (Fig. a).

    Techniques: Polymerase Chain Reaction

    Subject-derived E. faecium strains lead to different pathology and cytokine expression profiles in the colon. Fecal suspensions from selected subjects (HD55 and IBD36) and E. faecium strain IB18a, IB51a, or HD26a suspensions were transplanted or inoculated into microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Mean pathology scores of mice from each treatment group. d mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. e , f Suspension of HD55 or IBD51 feces or strain IB51a was gavaged into germ-free Il10 −/− mice. e Mean pathology scores of mice from each treatment group. f mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a – f are the mean ± SE. Statistical differences between a value and the HD55 control were analyzed using the Kruskal-Wallis test followed by Dunn’s test. * P

    Journal: Genome Biology

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    doi: 10.1186/s13059-019-1879-9

    Figure Lengend Snippet: Subject-derived E. faecium strains lead to different pathology and cytokine expression profiles in the colon. Fecal suspensions from selected subjects (HD55 and IBD36) and E. faecium strain IB18a, IB51a, or HD26a suspensions were transplanted or inoculated into microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Mean pathology scores of mice from each treatment group. d mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. e , f Suspension of HD55 or IBD51 feces or strain IB51a was gavaged into germ-free Il10 −/− mice. e Mean pathology scores of mice from each treatment group. f mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a – f are the mean ± SE. Statistical differences between a value and the HD55 control were analyzed using the Kruskal-Wallis test followed by Dunn’s test. * P

    Article Snippet: The genotypes of 10 analyzed E. faecium strains were different and could be separated into two major clusters: one containing two probiotic strains and the other containing pro-inflammatory strain ATCC 19434 (Fig. a).

    Techniques: Derivative Assay, Expressing, Mouse Assay, Transplantation Assay, Real-time Polymerase Chain Reaction

    Genome analysis of 10 E. faecium strains reveals inflammatory and probiotic clusters. a Three (HD26a, HD50a, and HD59a) and 4 (IB6a, IB18a, IB44a, and IB50a) E. faecium strains were isolated from the feces of HD subjects and UC patients, respectively. The genotypes of 10 E. faecium strains, including the 3 HD-derived and 4 UC-derived strains, inflammatory strain ATCC 19434, and probiotic strains NCIMB 11181 and SF68, were examined by sequencing. All 1683 identified genes (except for those coding for hypothetical proteins) were used for hierarchical clustering analysis of the 10 E. faecium strains. b LDA was performed using LEfSe to identify significant differences in KEGG-based metabolic pathways in the genomes of the 10 strains to compare between the inflammatory cluster in which ATCC 19434 was included and the probiotic cluster in which NCIMB 11181 and SF68 were included. Differentially abundant pathways for which the corresponding LDA scores indicate P

    Journal: Genome Biology

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    doi: 10.1186/s13059-019-1879-9

    Figure Lengend Snippet: Genome analysis of 10 E. faecium strains reveals inflammatory and probiotic clusters. a Three (HD26a, HD50a, and HD59a) and 4 (IB6a, IB18a, IB44a, and IB50a) E. faecium strains were isolated from the feces of HD subjects and UC patients, respectively. The genotypes of 10 E. faecium strains, including the 3 HD-derived and 4 UC-derived strains, inflammatory strain ATCC 19434, and probiotic strains NCIMB 11181 and SF68, were examined by sequencing. All 1683 identified genes (except for those coding for hypothetical proteins) were used for hierarchical clustering analysis of the 10 E. faecium strains. b LDA was performed using LEfSe to identify significant differences in KEGG-based metabolic pathways in the genomes of the 10 strains to compare between the inflammatory cluster in which ATCC 19434 was included and the probiotic cluster in which NCIMB 11181 and SF68 were included. Differentially abundant pathways for which the corresponding LDA scores indicate P

    Article Snippet: The genotypes of 10 analyzed E. faecium strains were different and could be separated into two major clusters: one containing two probiotic strains and the other containing pro-inflammatory strain ATCC 19434 (Fig. a).

    Techniques: Isolation, Derivative Assay, Sequencing

    a Blast atlas of 10 Enterococcus hirae strains isolated from bovine feces and E. hirae strain R17 mapped against E. hirae ATCC9790. b Blast atlas of the genomes of 3 Entercoccus faecium isolates from bovine feces and 12 complete E. faecium genomes from the NCBI database mapped against reference sequence E. faecium DO. Blast atlases were generated by GView Java package software [ 28 ] using both alignment length and percent identity cut-off values of 80%. Based on the reference genomes, phage and transposon related regions/loci are indicated on the altas diagram

    Journal: BMC Microbiology

    Article Title: Comparative genomics of Enterococcus spp. isolated from bovine feces

    doi: 10.1186/s12866-017-0962-1

    Figure Lengend Snippet: a Blast atlas of 10 Enterococcus hirae strains isolated from bovine feces and E. hirae strain R17 mapped against E. hirae ATCC9790. b Blast atlas of the genomes of 3 Entercoccus faecium isolates from bovine feces and 12 complete E. faecium genomes from the NCBI database mapped against reference sequence E. faecium DO. Blast atlases were generated by GView Java package software [ 28 ] using both alignment length and percent identity cut-off values of 80%. Based on the reference genomes, phage and transposon related regions/loci are indicated on the altas diagram

    Article Snippet: The 27 compete genomes from NCBI included: E. hirae (2 strains; ATCC 9790, R17), E. faecium (13 strains; Aus0004, Aus0085, T110, 6E6, VRE001, E1, E745, E39, UW8175, NRRL B2354, ATCC 700221, EFE10021), E. faecalis (9 strains; LD33, L12, KB1, 62, D32, V583, DENG1, OG1RF, ATCC 29212), E. durans (1 strain; KLDS6_0933), E. gallinarum (1 strain; FDAARGOS163), and E. casseliflavus (1 strain; EC20).

    Techniques: Isolation, Sequencing, Generated, Software

    a Phylogenetic tree of Entercoccus faecium genome sequences from the present study and complete genome sequences from the NCBI database based on analysis of single-nucleotide varients (SNVs) of the core genes. b Relatedness tree of E. faecium genome sequences from present study and complete genome sequences from the NCBI database based on Pearson correlation similarity matrix analysis of accessory genes. Origin of isolates are as indicated in the figures

    Journal: BMC Microbiology

    Article Title: Comparative genomics of Enterococcus spp. isolated from bovine feces

    doi: 10.1186/s12866-017-0962-1

    Figure Lengend Snippet: a Phylogenetic tree of Entercoccus faecium genome sequences from the present study and complete genome sequences from the NCBI database based on analysis of single-nucleotide varients (SNVs) of the core genes. b Relatedness tree of E. faecium genome sequences from present study and complete genome sequences from the NCBI database based on Pearson correlation similarity matrix analysis of accessory genes. Origin of isolates are as indicated in the figures

    Article Snippet: The 27 compete genomes from NCBI included: E. hirae (2 strains; ATCC 9790, R17), E. faecium (13 strains; Aus0004, Aus0085, T110, 6E6, VRE001, E1, E745, E39, UW8175, NRRL B2354, ATCC 700221, EFE10021), E. faecalis (9 strains; LD33, L12, KB1, 62, D32, V583, DENG1, OG1RF, ATCC 29212), E. durans (1 strain; KLDS6_0933), E. gallinarum (1 strain; FDAARGOS163), and E. casseliflavus (1 strain; EC20).

    Techniques:

    Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis , Entercoccus faecium , Enterococcus hirae , Entercoccus durans , Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain T110 as a reference

    Journal: BMC Microbiology

    Article Title: Comparative genomics of Enterococcus spp. isolated from bovine feces

    doi: 10.1186/s12866-017-0962-1

    Figure Lengend Snippet: Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis , Entercoccus faecium , Enterococcus hirae , Entercoccus durans , Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain T110 as a reference

    Article Snippet: The 27 compete genomes from NCBI included: E. hirae (2 strains; ATCC 9790, R17), E. faecium (13 strains; Aus0004, Aus0085, T110, 6E6, VRE001, E1, E745, E39, UW8175, NRRL B2354, ATCC 700221, EFE10021), E. faecalis (9 strains; LD33, L12, KB1, 62, D32, V583, DENG1, OG1RF, ATCC 29212), E. durans (1 strain; KLDS6_0933), E. gallinarum (1 strain; FDAARGOS163), and E. casseliflavus (1 strain; EC20).

    Techniques: Construct

    Adoptive transfer of bacterial consortia containing C. bolteae and B. producta prevents VRE colonization (A–B) Ampicillin-treated mice were orally gavaged with PBS or a mixture of C. bolteae , B. producta , Blautia_unclassified, E. dolichum , A. muciniphila , P. distasonis and B. sartorii (7-mix) for three consecutive days beginning on day 2 of antibiotic treatment. Mice were challenged with VRE the day following the third gavage and fecal samples were collected at the indicated time points. (A) VRE CFUs in stool samples 1, 3 and 6/8 days post inoculation (p.i.) (**** P

    Journal: Cell host & microbe

    Article Title: Cooperating commensals restore colonization resistance to vancomycin- resistant Enterococcus faecium

    doi: 10.1016/j.chom.2017.04.002

    Figure Lengend Snippet: Adoptive transfer of bacterial consortia containing C. bolteae and B. producta prevents VRE colonization (A–B) Ampicillin-treated mice were orally gavaged with PBS or a mixture of C. bolteae , B. producta , Blautia_unclassified, E. dolichum , A. muciniphila , P. distasonis and B. sartorii (7-mix) for three consecutive days beginning on day 2 of antibiotic treatment. Mice were challenged with VRE the day following the third gavage and fecal samples were collected at the indicated time points. (A) VRE CFUs in stool samples 1, 3 and 6/8 days post inoculation (p.i.) (**** P

    Article Snippet: For VRE challenge, 6–8 week old female C57BL/6 mice from Jackson Laboratories were treated with 0.5 g/L ampicillin (Fisher) in their drinking water and inoculated with 5x104 VRE CFUs ( E. faecium , ATCC 700221) in 200μl by gavage.

    Techniques: Adoptive Transfer Assay, Mouse Assay

    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min

    Journal: BMC Microbiology

    Article Title: Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains

    doi: 10.1186/s12866-018-1321-6

    Figure Lengend Snippet: Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min

    Article Snippet: Both E. faecium strains had the lowest biofilm mass in MHB compared with other tested strains, with an average of 0.149 for E. faecium ATCC 35667 (Fig. ) and 0.109 for vancomycin resistant E. faecium (VRE) ATCC 700221 (Fig. ).

    Techniques: Activity Assay, Incubation, Fluorescence

    Assessment of biofilm biomass by crystal violet staining and cell enumeration by colony count. Biofilm production analysis through crystal violet staining (OD 570 nm, bars) and cell enumeration (log 10 CFU/mL, lines) of six bacterial strains: ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212 and ( f ) Enterococcus faecalis VRE ATCC 51575, incubated in six different media: Mueller Hinton (MH), Tryptic Soy (TS), Tryptic Soy supplemented with 1% glucose (TSG), Tryptic Soy supplemented with 2% glucose (TS2G), Brain Heart Infusion (BHI) and Brain Heart Infusion supplemented with 1% glucose (BHIG). Error bars represent standard deviation of two independent experiments in triplicate. Lowercase letters indicate significant differences amongst media used for biofilm production. Similar letters denote no significant difference ( P > 0.05)

    Journal: BMC Microbiology

    Article Title: Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains

    doi: 10.1186/s12866-018-1321-6

    Figure Lengend Snippet: Assessment of biofilm biomass by crystal violet staining and cell enumeration by colony count. Biofilm production analysis through crystal violet staining (OD 570 nm, bars) and cell enumeration (log 10 CFU/mL, lines) of six bacterial strains: ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212 and ( f ) Enterococcus faecalis VRE ATCC 51575, incubated in six different media: Mueller Hinton (MH), Tryptic Soy (TS), Tryptic Soy supplemented with 1% glucose (TSG), Tryptic Soy supplemented with 2% glucose (TS2G), Brain Heart Infusion (BHI) and Brain Heart Infusion supplemented with 1% glucose (BHIG). Error bars represent standard deviation of two independent experiments in triplicate. Lowercase letters indicate significant differences amongst media used for biofilm production. Similar letters denote no significant difference ( P > 0.05)

    Article Snippet: Both E. faecium strains had the lowest biofilm mass in MHB compared with other tested strains, with an average of 0.149 for E. faecium ATCC 35667 (Fig. ) and 0.109 for vancomycin resistant E. faecium (VRE) ATCC 700221 (Fig. ).

    Techniques: Staining, Incubation, Standard Deviation