e faecium do  (ATCC)


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    Name:
    Enterococcus faecium TEX16 TX0016
    Description:

    Catalog Number:
    baa-472
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    Structured Review

    ATCC e faecium do
    PFGE analysis of E. <t>faecium</t> isolates evaluated in this study. Analysis was performed using BioNumerics software. Dendrograms were generated using the unweighted-pair group method using average linkages and band-based Dice similarity coefficients. The optimization parameter for this evaluation was set to 0.5 and band position tolerance to 1.0%. Isolate 5 results are not shown in this figure, but that isolate exhibited a restriction pattern identical to that of isolate 4.

    https://www.bioz.com/result/e faecium do/product/ATCC
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    e faecium do - by Bioz Stars, 2020-07
    90/100 stars

    Images

    1) Product Images from "Mechanisms of Resistance to Daptomycin in Enterococcus faecium ▿"

    Article Title: Mechanisms of Resistance to Daptomycin in Enterococcus faecium ▿

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00774-07

    PFGE analysis of E. faecium isolates evaluated in this study. Analysis was performed using BioNumerics software. Dendrograms were generated using the unweighted-pair group method using average linkages and band-based Dice similarity coefficients. The optimization parameter for this evaluation was set to 0.5 and band position tolerance to 1.0%. Isolate 5 results are not shown in this figure, but that isolate exhibited a restriction pattern identical to that of isolate 4.
    Figure Legend Snippet: PFGE analysis of E. faecium isolates evaluated in this study. Analysis was performed using BioNumerics software. Dendrograms were generated using the unweighted-pair group method using average linkages and band-based Dice similarity coefficients. The optimization parameter for this evaluation was set to 0.5 and band position tolerance to 1.0%. Isolate 5 results are not shown in this figure, but that isolate exhibited a restriction pattern identical to that of isolate 4.

    Techniques Used: Software, Generated

    Diameter of zone of inhibition as a function of incubation time with different E. faecium strains. No significant difference was observed between the strains tested and the control solution with daptomycin and tryptic soy broth. For isolate 1, the daptomycin MIC was 4 μg/ml; for isolate 8, the MIC was 16 μg/ml; and for E. faecium ATCC 35667, the MIC was 1 μg/ml).
    Figure Legend Snippet: Diameter of zone of inhibition as a function of incubation time with different E. faecium strains. No significant difference was observed between the strains tested and the control solution with daptomycin and tryptic soy broth. For isolate 1, the daptomycin MIC was 4 μg/ml; for isolate 8, the MIC was 16 μg/ml; and for E. faecium ATCC 35667, the MIC was 1 μg/ml).

    Techniques Used: Inhibition, Incubation

    Related Articles

    Sequencing:

    Article Title: Mechanisms of Resistance to Daptomycin in Enterococcus faecium ▿
    Article Snippet: .. Sequences from these two strains were identical to each other and to the genome sequence of E. faecium DO (ATCC BAA-472), suggesting an alternative resistance mechanism. ..

    Negative Control:

    Article Title: The Genus Enterococcus: Between Probiotic Potential and Safety Concerns—An Update
    Article Snippet: .. Both analysis should contain positive E. faecium ATCC BAA-472 (TX16) or E. faecium DSMZ 25390) and negative control ( E. faecium DSMZ 25389) strains (EFSA, ). .. For instance, the determination of antibiotic's (mainly ampicillin) minimum inhibitory concentration (MIC) should be performed according to internationally recognized standards such as European Union Committee on Antimicrobial Susceptibility Testing (EUCAST), the Clinical and Laboratory Standard Institute (CLSI), ISO standard or similar.

    Article Title: The Genus Enterococcus: Between Probiotic Potential and Safety Concerns—An Update
    Article Snippet: .. Both analysis should contain positive E. faecium ATCC BAA-472 (TX16) or E. faecium DSMZ 25390) and negative control (E. faecium DSMZ 25389) strains (EFSA, ). .. For instance, the determination of antibiotic's (mainly ampicillin) minimum inhibitory concentration (MIC) should be performed according to internationally recognized standards such as European Union Committee on Antimicrobial Susceptibility Testing (EUCAST), the Clinical and Laboratory Standard Institute (CLSI), ISO standard or similar.

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    ATCC e faecium do
    Growth curves of Enterococcus faecalis OG1RF and Enterococcus <t>faecium</t> 64/3 and their optrA transconjugants
    E Faecium Do, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e faecium do/product/ATCC
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    e faecium do - by Bioz Stars, 2020-07
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    Image Search Results


    Growth curves of Enterococcus faecalis OG1RF and Enterococcus faecium 64/3 and their optrA transconjugants

    Journal: European Journal of Clinical Microbiology & Infectious Diseases

    Article Title: Linezolid-resistant enterococci in Polish hospitals: species, clonality and determinants of linezolid resistance

    doi: 10.1007/s10096-017-2934-7

    Figure Lengend Snippet: Growth curves of Enterococcus faecalis OG1RF and Enterococcus faecium 64/3 and their optrA transconjugants

    Article Snippet: The rplC and rplD genes encoding ribosomal proteins L3 and L4, respectively, were sequenced [ ] and compared to the wild-type sequences from E. faecalis ATCC 29212 (CP008816.1), E. faecium DO (CP003583.1) and E. avium ATCC 14025 (ASWL01000001.1) strains.

    Techniques:

    NeighborNet phylogenetic network to visualize the relationships between 176 E. faecium isolates. The distance matrix underlying the network was built from all pairwise allelic profile comparisons. Allelic profiles were extracted from SeqSphere+. E. faecium

    Journal: Journal of Clinical Microbiology

    Article Title: Core Genome Multilocus Sequence Typing Scheme for High-Resolution Typing of Enterococcus faecium

    doi: 10.1128/JCM.01946-15

    Figure Lengend Snippet: NeighborNet phylogenetic network to visualize the relationships between 176 E. faecium isolates. The distance matrix underlying the network was built from all pairwise allelic profile comparisons. Allelic profiles were extracted from SeqSphere+. E. faecium

    Article Snippet: These plasmids came from the following strains (with GenBank accession numbers): Enterococcus faecalis D32 ( and ), E. faecalis V583 ( , , and ), E. faecium Aus0004 ( , , and ), E. faecium Aus0085 ( , , , , , and ), E. faecium DO ( , , and ), E. faecium NRRL B-2354 , Enterococcus hirae ATCC 9790 , and Enterococcus mundtii QU 25 ( , , , , and ).

    Techniques:

    Minimum spanning network built from the core genome allelic profiles of 103 clinical E. faecium isolates. The core genome allelic profiles were determined by loading de novo assemblies into the E. faecium cgMLST scheme developed in this study using the

    Journal: Journal of Clinical Microbiology

    Article Title: Core Genome Multilocus Sequence Typing Scheme for High-Resolution Typing of Enterococcus faecium

    doi: 10.1128/JCM.01946-15

    Figure Lengend Snippet: Minimum spanning network built from the core genome allelic profiles of 103 clinical E. faecium isolates. The core genome allelic profiles were determined by loading de novo assemblies into the E. faecium cgMLST scheme developed in this study using the

    Article Snippet: These plasmids came from the following strains (with GenBank accession numbers): Enterococcus faecalis D32 ( and ), E. faecalis V583 ( , , and ), E. faecium Aus0004 ( , , and ), E. faecium Aus0085 ( , , , , , and ), E. faecium DO ( , , and ), E. faecium NRRL B-2354 , Enterococcus hirae ATCC 9790 , and Enterococcus mundtii QU 25 ( , , , , and ).

    Techniques:

    Allele-based neighbor-joining (NJ) and SNP-based maximum likelihood (ML) trees of 103 clinical E. faecium isolates. The allele-based NJ tree (left) was built using the E. faecium cgMLST scheme developed in this study using the SeqSphere+ software. The

    Journal: Journal of Clinical Microbiology

    Article Title: Core Genome Multilocus Sequence Typing Scheme for High-Resolution Typing of Enterococcus faecium

    doi: 10.1128/JCM.01946-15

    Figure Lengend Snippet: Allele-based neighbor-joining (NJ) and SNP-based maximum likelihood (ML) trees of 103 clinical E. faecium isolates. The allele-based NJ tree (left) was built using the E. faecium cgMLST scheme developed in this study using the SeqSphere+ software. The

    Article Snippet: These plasmids came from the following strains (with GenBank accession numbers): Enterococcus faecalis D32 ( and ), E. faecalis V583 ( , , and ), E. faecium Aus0004 ( , , and ), E. faecium Aus0085 ( , , , , , and ), E. faecium DO ( , , and ), E. faecium NRRL B-2354 , Enterococcus hirae ATCC 9790 , and Enterococcus mundtii QU 25 ( , , , , and ).

    Techniques: Software

    Distribution of allele differences (A) and recombination-filtered SNPs (B) for pairs of E. faecium isolates with the same ST and isolated from the same hospital. The distributions represent 1,073 pairwise comparisons. Allelic profiles were extracted from

    Journal: Journal of Clinical Microbiology

    Article Title: Core Genome Multilocus Sequence Typing Scheme for High-Resolution Typing of Enterococcus faecium

    doi: 10.1128/JCM.01946-15

    Figure Lengend Snippet: Distribution of allele differences (A) and recombination-filtered SNPs (B) for pairs of E. faecium isolates with the same ST and isolated from the same hospital. The distributions represent 1,073 pairwise comparisons. Allelic profiles were extracted from

    Article Snippet: These plasmids came from the following strains (with GenBank accession numbers): Enterococcus faecalis D32 ( and ), E. faecalis V583 ( , , and ), E. faecium Aus0004 ( , , and ), E. faecium Aus0085 ( , , , , , and ), E. faecium DO ( , , and ), E. faecium NRRL B-2354 , Enterococcus hirae ATCC 9790 , and Enterococcus mundtii QU 25 ( , , , , and ).

    Techniques: Isolation

    Analysis of the Enterococcus mundtii QU 25 complete genome. (A) Linearized Nco I-digested whole-genome optical map of QU 25 compared with the in silico -derived Nco I-digest map, demonstrating correct chromosome assembly. (B) Genome map of the QU 25 strain. In the outermost circle, three prophages of phiEmqu1, phiEmqu2, and phiEmqu3, replication origin ( dnaA ), and terminus ( dif ) are shown. In the second circle, the ORFs transcribed in a clockwise manner are shown as bars. The third circle shows ORFS transcribed in a counter-clockwise manner. The fourth to ninth circles depict the results of ortholog analyses (BLASTP E -value ≤1 × 10 −10 ) with E. mundtii ATCC 882, E. faecium DO, E. faecium Aus0004, E. hirae ATCC 9790, E. casseliflavus EC20, and E. faecalis V583, respectively. The extent of homology relative to QU 25 is depicted using a heat map of arbitrarily chosen bins. The colour scheme and percentage identity for orthologs are as follows: red, orthologs with > 90% identity; green, 70–90% identity; blue, 50–70% identity; and black,

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: Complete Genome Sequence of Enterococcus mundtii QU 25, an Efficient l-(+)-Lactic Acid-Producing Bacterium

    doi: 10.1093/dnares/dsu003

    Figure Lengend Snippet: Analysis of the Enterococcus mundtii QU 25 complete genome. (A) Linearized Nco I-digested whole-genome optical map of QU 25 compared with the in silico -derived Nco I-digest map, demonstrating correct chromosome assembly. (B) Genome map of the QU 25 strain. In the outermost circle, three prophages of phiEmqu1, phiEmqu2, and phiEmqu3, replication origin ( dnaA ), and terminus ( dif ) are shown. In the second circle, the ORFs transcribed in a clockwise manner are shown as bars. The third circle shows ORFS transcribed in a counter-clockwise manner. The fourth to ninth circles depict the results of ortholog analyses (BLASTP E -value ≤1 × 10 −10 ) with E. mundtii ATCC 882, E. faecium DO, E. faecium Aus0004, E. hirae ATCC 9790, E. casseliflavus EC20, and E. faecalis V583, respectively. The extent of homology relative to QU 25 is depicted using a heat map of arbitrarily chosen bins. The colour scheme and percentage identity for orthologs are as follows: red, orthologs with > 90% identity; green, 70–90% identity; blue, 50–70% identity; and black,

    Article Snippet: The relatively closed species, such as E. mundtii ATCC 882, E. faecium DO and Aus0004 strains, and E. hirae ATCC 9790, have 21, 180, 76, and 14 ISEs and transposase-related genes, respectively.

    Techniques: In Silico, Derivative Assay

    Circular map of the Enterococcus hirae R17 genome. Circles from outside to inside are as follows: (1) scale marks of the E. hirae R17 genome. The blue rectangles represent genomic islands ( GI 1 through GI 12), gray rectangles represent genomic regions unique in E. hirae R17 compared to E. hirae ATCC ™ 9790 (R1 through R9), and red rectangles represent genomic regions unique in E. hirae R17 compared to E. hirae ATCC ™ 9790, Enterococcus faecium DO , and Enterococcus faecalis V583 (R#1 through R#5); (2) and (3) predicted proteins encoded by genes on the forward and reverse strands; (4), (5), and (6): common genes shared by E. hirae R17 with those of reference genomes E. hirae ATCC ™ 9790, E. faecium DO , and E. faecalis V583, respectively; (7) GC content percentage, above median GC content (red), less than median (blue); (8) GC skew [(G‐C)/(G+C)], values > 0 (red), values

    Journal: MicrobiologyOpen

    Article Title: Genomic insights into the pathogenicity and environmental adaptability of Enterococcus hirae R17 isolated from pork offered for retail sale, et al. Genomic insights into the pathogenicity and environmental adaptability of Enterococcus hirae R17 isolated from pork offered for retail sale

    doi: 10.1002/mbo3.514

    Figure Lengend Snippet: Circular map of the Enterococcus hirae R17 genome. Circles from outside to inside are as follows: (1) scale marks of the E. hirae R17 genome. The blue rectangles represent genomic islands ( GI 1 through GI 12), gray rectangles represent genomic regions unique in E. hirae R17 compared to E. hirae ATCC ™ 9790 (R1 through R9), and red rectangles represent genomic regions unique in E. hirae R17 compared to E. hirae ATCC ™ 9790, Enterococcus faecium DO , and Enterococcus faecalis V583 (R#1 through R#5); (2) and (3) predicted proteins encoded by genes on the forward and reverse strands; (4), (5), and (6): common genes shared by E. hirae R17 with those of reference genomes E. hirae ATCC ™ 9790, E. faecium DO , and E. faecalis V583, respectively; (7) GC content percentage, above median GC content (red), less than median (blue); (8) GC skew [(G‐C)/(G+C)], values > 0 (red), values

    Article Snippet: 2.4 Comparative genomic analysis Three complete and annotated genomes of E. faecium DO (accession number from CP003583 to CP003586), E. faecalis V583 (accession number from AE016830 to AE016833), and E. hirae ATCC™ 9790 (accession number CP003504.1 and HQ724512.1) were available from GenBank and used as reference sequences in this study.

    Techniques: